FlyBase Allele Report: Dmel\pins[193]

General Information

Symbol

Dmel\pins¹⁹³

Species

D. melanogaster

Name

FlyBase ID

FBal0117396

Feature type

allele

Associated gene

Dmel\pins

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Parmentier et al., 2000)

Mutagen

Delta2-3 transposase

(Parmentier et al., 2000)

Progenitor genotype

P{lacW}wdb^S031807a

(Parmentier et al., 2000)

Caused by aberration

Df(3R)raps¹⁹³

Cytology

Description

Imprecise excision of the P{lacW} element, resulting in a 2658bp deletion affecting both the wdb and raps transcription units.

(Parmentier et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(Katanaev and Tomlinson, 2006)

abnormal mitotic cell cycle (with pins^Δ50)

(Katanaev and Tomlinson, 2006)

lethal - all die before end of pupal stage | recessive

(Parmentier et al., 2000)

some die during pupal stage | recessive

(Parmentier et al., 2000)

Phenotype Manifest In

neuroblast | larval stage

(Parmentier et al., 2000)

wing margin (with pins^Δ50)

(Katanaev and Tomlinson, 2006)

Detailed Description

Statement

Reference

raps¹⁹³ homozygous mutants exhibit extensive asymmetric division phenotypes in all tissues analysed. raps^Δ50/raps¹⁹³ transheterozygous mutants exhibit extensive asymmetric division phenotypes in all tissues analysed. These phenotypes can be rescued through expression of the raps^hs.PP transgene.

(Katanaev and Tomlinson, 2006)

Homozygotes die as young pupae. 28% of neuroblast divisions are morphologically symmetrical in third instar mutant larvae, in contrast to wild type, where all neuroblast divisions are morphologically asymmetric. The neuroblasts of third-instar larvae are smaller than in wild type, and their size approaches that of ganglion mother cells.

(Parmentier et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

pins¹⁹³ has abnormal mitotic cell cycle phenotype, enhanceable by Gαo^UAS.cKa/Scer\GAL4^Bx-MS1096

(Katanaev and Tomlinson, 2006)

Enhancer of

Statement

Reference

raps[+]/pins¹⁹³ is an enhancer of abnormal planar polarity phenotype of Gαo^UAS.cKa, Scer\GAL4^Bx-MS1096

(Katanaev and Tomlinson, 2006)

Suppressor of

Statement

Reference

raps[+]/pins¹⁹³ is a suppressor of increased cell death | heat sensitive phenotype of aPKC^ts/aPKC^k06403

(Guilgur et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

raps[+]/pins¹⁹³ is a suppressor of wing disc | third instar larval stage | heat sensitive phenotype of aPKC^ts/aPKC^k06403

(Guilgur et al., 2012)

raps[+]/pins¹⁹³ is a suppressor of embryo | dorsal closure stage | heat sensitive phenotype of aPKC^ts/aPKC^k06403

(Guilgur et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

raps¹⁹³ dominantly suppresses the temperature sensitive induction of apoptosis seen in aPKC^ts/aPKC^k06403 third instar larval wing discs at the semi-permissive temperature (28[o]C). However, this suppression does not occur at the restrictive temperature (30[o]C).

raps¹⁹³ dominantly suppresses the abdominal dorsal closure defects seen in aPKC^ts/aPKC^k06403 mutant flies at the semi-permissive temperature (28[o]C). However, this suppression did not occur at the restrictive temperature (30[o]C).

(Guilgur et al., 2012)

Overexpression of G-oα47A^Scer\UAS.cKa, under the control of Scer\GAL4^Bx-MS1096 in a raps¹⁹³ heterozygous background results in a significant enhancement of the asymmetric division defects observed in G-oα47A^Scer\UAS.cKa Scer\GAL4^Bx-MS1096 mutants.

(Katanaev and Tomlinson, 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

pins¹⁹³ is rescued by pins^hs.PP

(Katanaev and Tomlinson, 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6491

w^*; pins¹⁹³/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

193

(Bell et al., 2015)

pins¹⁹³

(Bergstralh et al., 2016, Guilgur et al., 2012)

pins^raps193

(Katanaev and Tomlinson, 2006)

raps¹⁹³

(Schulman et al., 2014, Cook et al., 2009.2.28, Christensen et al., 2008.4.15, Christensen et al., 2008.4.15, Cook et al., 2008.12.28)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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