FlyBase Allele Report: Dmel\Magi[UAS.GFP]

General Information

Symbol

Dmel\Magi^UAS.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0322790

Feature type

allele

Associated gene

Dmel\Magi

Associated Insertion(s)

Carried in Construct

M{UAS-Magi.GFP}

Key Links

Transgenic product class

cDNA

(Zaessinger et al., 2015)

wild_type

(Zaessinger et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Zaessinger et al., 2015)

wild_type

(Zaessinger et al., 2015)

Mutagen

in vitro construct

(Zaessinger et al., 2015)

Progenitor genotype

Carried in construct

M{UAS-Magi.GFP}

Cytology

Description

UASt regulatory sequences drive expression of the full-length Magi coding sequence (derived from the LD27118 cDNA clone) tagged at the N-terminal end with GFP.

(Zaessinger et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Magi

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible | adult stage, with Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Phenotype Manifest In

eye | adult stage, with Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

interommatidial cell | pharate adult stage, with Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Detailed Description

Statement

Reference

Expression of Magi^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PU results in a loss of inter-ommatidial cells in pharate adults (not due to increased apoptosis however) and a mild rough eye phenotype in the adults.

(Zaessinger et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by RASSF8^UASp.cLa, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by RASSF8⁴, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by sav[+]/sav³

(Zaessinger et al., 2015)

NOT Enhanced by

Statement

Reference

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by Akt1[+]/Akt⁰⁴²²⁶

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by Df(1)BSC538/+

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by ex¹/ex[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by ex⁶⁹⁷/ex[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by kibra[+]/kibra^del

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by sav[+]/sav⁴

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by wts^x1/wts[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by wts[+]/wts^M541

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by yki[+]/yki^B5

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by ASPP^d/ASPP[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by ASPP[+]/ASPP⁸

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by Csk^j1D8/Csk[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by puc^A251.1F3/puc[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by pyd^tam/pyd[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by pyd^ex147/pyd[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by aPKC^k06403/aPKC[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by Pten^DS189/Pten[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by Pten^c494/Pten[+]

(Zaessinger et al., 2015)

Suppressed by

Statement

Reference

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible by RASSF8[+]/RASSF8⁶

(Zaessinger et al., 2015)

NOT suppressed by

Statement

Reference

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by Pten^DS189/Pten[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by Pten^c494/Pten[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by yki[+]/yki^B5

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by ASPP^d/ASPP[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by ASPP[+]/ASPP⁸

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by Csk^j1D8/Csk[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by puc^A251.1F3/puc[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by pyd^tam/pyd[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by pyd^ex147/pyd[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by aPKC^k06403/aPKC[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by Akt1[+]/Akt⁰⁴²²⁶

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by Df(1)BSC538/+

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by ex¹/ex[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by ex⁶⁹⁷/ex[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by kibra[+]/kibra^del

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by sav[+]/sav⁴

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by wts^x1/wts[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by wts[+]/wts^M541

(Zaessinger et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, enhanceable by RASSF8^UASp.cLa, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, enhanceable by RASSF8⁴, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, enhanceable by sav[+]/sav³

(Zaessinger et al., 2015)

NOT Enhanced by

Statement

Reference

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-enhanceable by Akt1[+]/Akt⁰⁴²²⁶

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-enhanceable by Df(1)BSC538/+

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-enhanceable by ex¹/ex[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-enhanceable by ex⁶⁹⁷/ex[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-enhanceable by kibra[+]/kibra^del

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-enhanceable by sav[+]/sav⁴

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-enhanceable by wts^x1/wts[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-enhanceable by wts[+]/wts^M541

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-enhanceable by yki[+]/yki^B5

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-enhanceable by ASPP^d/ASPP[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-enhanceable by ASPP[+]/ASPP⁸

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-enhanceable by Csk^j1D8/Csk[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-enhanceable by puc^A251.1F3/puc[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-enhanceable by pyd^tam/pyd[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-enhanceable by pyd^ex147/pyd[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-enhanceable by aPKC^k06403/aPKC[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-enhanceable by Pten^DS189/Pten[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-enhanceable by Pten^c494/Pten[+]

(Zaessinger et al., 2015)

Suppressed by

Statement

Reference

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, suppressible by RASSF8[+]/RASSF8⁶

(Zaessinger et al., 2015)

NOT suppressed by

Statement

Reference

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-suppressible by Pten^DS189/Pten[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-suppressible by Pten^c494/Pten[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-suppressible by Akt1[+]/Akt⁰⁴²²⁶

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-suppressible by Df(1)BSC538/+

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-suppressible by ex¹/ex[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-suppressible by ex⁶⁹⁷/ex[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-suppressible by kibra[+]/kibra^del

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-suppressible by sav[+]/sav⁴

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-suppressible by wts^x1/wts[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-suppressible by wts[+]/wts^M541

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-suppressible by yki[+]/yki^B5

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-suppressible by ASPP^d/ASPP[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-suppressible by ASPP[+]/ASPP⁸

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-suppressible by Csk^j1D8/Csk[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-suppressible by puc^A251.1F3/puc[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-suppressible by pyd^tam/pyd[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-suppressible by pyd^ex147/pyd[+]

(Zaessinger et al., 2015)

Magi^UAS.GFP, Scer\GAL4^GMR.PU has eye | adult stage phenotype, non-suppressible by aPKC^k06403/aPKC[+]

(Zaessinger et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The rough eye phenotype in adult flies induced by expression of Magi^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PU is significantly suppressed by combination with a single copy of RASSF8⁶ and strongly exacerbated by co-expression of RASSF8^{Scer\UAS.P\T.cLa} or RASSF8⁴, the phenotype is also slightly worsened by combination with single copy of sav³/+.

The rough eye phenotype observed in adults expressing Magi^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PU is not modified by combination with single copy of any of the following alleles or deficiencies: Pten^DS189, Pten^c494, Df(2L)ED729, Akt1⁰⁴²²⁶, Df(1)BSC538, ex¹, ex⁶⁹⁷, kibra^del, sav⁴, wts^x1, wts^M541, yki^B5, ASPP^d, ASPP⁸, Csk^j1D8, puc^A251.1F3, pyd^tam, pyd^ex147 or aPKC^k06403.

(Zaessinger et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Magi^{Scer\UAS.T:Avic\GFP}

Magi^UAS.GFP

Name Synonyms

Secondary FlyBase IDs

References (1)

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