FlyBase Allele Report: Dmel\ex[697]

General Information

Symbol

Dmel\ex⁶⁹⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0031223

Feature type

allele

Associated gene

Dmel\ex

Associated Insertion(s)

P{lacW}ex⁶⁹⁷

Carried in Construct

Also Known As

ex-lacZ, Ex-Z, expanded-lacZ, ex-lacZ⁶⁹⁷, ex⁶⁹⁷-lacZ

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Boedigheimer and Laughon, 1993)

Progenitor genotype

Associated Insertion(s)

P{lacW}ex⁶⁹⁷

Cytology

Description

Insertion of P{lacW} at the 5'end of the ex transcription unit.

(Boedigheimer and Laughon, 1993)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:431,266..431,266 [+]

(Maitra et al., 2006, Boedigheimer and Laughon, 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates carcinoma

modeled by Csk^Q156Stop, Ras85D^V12.UAS

(Hirabayashi and Cagan, 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

anterior crossvein

(McCartney et al., 2000)

eye-antennal disc | third instar larval stage

(Maitra et al., 2006)

interommatidial bristle & eye disc & pupa

(Maitra et al., 2006)

leg

(Boedigheimer and Laughon, 1993)

posterior crossvein

(McCartney et al., 2000)

thorax

(Boedigheimer and Laughon, 1993)

wing

(Yang and Choi, 2021, McCartney et al., 2000, Boedigheimer and Laughon, 1993)

wing disc

(Maitra et al., 2006)

Detailed Description

Statement

Reference

In ex⁶⁹⁷ third instar eye-antennal discs, many cells posterior to the second mitotic wave fail to exit the cell cycle.

In ex⁶⁹⁷ pupal eye discs (24hrs after pupariation) exhibit extra interommatidial cells, often forming double rows surrounding the ommatidia. The number of cone cells does not change in the mutants.

ex⁶⁹⁷ mutants exhibit overgrown wing imaginal discs.

(Maitra et al., 2006)

ex⁶⁹⁷ mutants do not exhibit any defect in photoreceptor differentiation.

(Maitra et al., 2006)

Homozygous females have a broad wing phenotype characterised by disruption of the posterior and anterior crossveins. The increased wing size appears to be due to an increase in cell number rather than an increase in cell size and in fact cell size is slightly decreased compared to wild type.

(McCartney et al., 2000)

Mild phenotype.

(Boedigheimer et al., 1997)

Broad, arced wings. Mutant wings have undergone at least one extra cell division. Legs, thorax and head may also be affected.

(Boedigheimer and Laughon, 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

ex⁶⁹⁷ has visible | recessive phenotype, enhanceable by Mer¹/Mer[+]

(McCartney et al., 2000)

Enhancer of

Statement

Reference

ex⁶⁹⁷/ex[+] is an enhancer of partially lethal - majority die | larval stage phenotype of wts^P2

(Hamaratoglu et al., 2006)

ex⁶⁹⁷ is an enhancer of visible | recessive phenotype of Mer³

(McCartney et al., 2000)

NOT Enhancer of

Statement

Reference

ex⁶⁹⁷/ex[+] is a non-enhancer of visible | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Suppressor of

Statement

Reference

ex⁶⁹⁷ is a suppressor of decreased size | adult stage phenotype of Patronin^GD11946, Scer\GAL4^en.PU

(Yang and Choi, 2021)

ex⁶⁹⁷ is a suppressor of visible | adult stage phenotype of Patronin^GD11946, Scer\GAL4^en.PU

(Yang and Choi, 2021)

NOT Suppressor of

Statement

Reference

ex⁶⁹⁷/ex[+] is a non-suppressor of visible | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Other

Statement

Reference

dpp^hr56, ex⁶⁹⁷ has visible phenotype

(McCartney et al., 2000)

dpp^hr56, ex⁶⁹⁷ has visible | recessive phenotype

(McCartney et al., 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

ex⁶⁹⁷ has wing phenotype, enhanceable by Mer¹/Mer[+]

(McCartney et al., 2000)

Enhancer of

Statement

Reference

ex⁶⁹⁷/ex[+] is an enhancer of wing vein | ectopic phenotype of Snr1^E1

(Marenda et al., 2004)

ex⁶⁹⁷ is an enhancer of eye phenotype of Mer³

(McCartney et al., 2000)

ex⁶⁹⁷ is an enhancer of vibrissal bristle | ectopic phenotype of Mer³

(McCartney et al., 2000)

ex⁶⁹⁷ is an enhancer of adult cuticle & head phenotype of Mer³

(McCartney et al., 2000)

ex⁶⁹⁷ is an enhancer of interommatidial bristle phenotype of Mer³

(McCartney et al., 2000)

ex⁶⁹⁷ is an enhancer of interommatidial bristle | ectopic phenotype of Mer³

(McCartney et al., 2000)

NOT Enhancer of

Statement

Reference

ex⁶⁹⁷/ex[+] is a non-enhancer of eye | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Suppressor of

Statement

Reference

ex⁶⁹⁷ is a suppressor of wing phenotype of Patronin^GD11946, Scer\GAL4^en.PU

(Yang and Choi, 2021)

NOT Suppressor of

Statement

Reference

ex⁶⁹⁷/ex[+] is a non-suppressor of eye | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Other

Statement

Reference

Mer⁴, ex⁶⁹⁷ has wing disc phenotype

(Maitra et al., 2006)

Mer⁴, ex⁶⁹⁷ has eye photoreceptor cell | somatic clone phenotype

(Maitra et al., 2006)

Mer⁴, ex⁶⁹⁷ has antennal disc | somatic clone phenotype

(Maitra et al., 2006)

Mer⁴, ex⁶⁹⁷ has antennal disc | ectopic | somatic clone phenotype

(Maitra et al., 2006)

Mer⁴, ex⁶⁹⁷ has embryonic primordium of adult eye | somatic clone phenotype

(Maitra et al., 2006)

dpp^hr56, ex⁶⁹⁷ has tarsal segment phenotype

(McCartney et al., 2000)

dpp^hr56, ex⁶⁹⁷ has leg phenotype

(McCartney et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The rough eye phenotype observed in adults expressing Magi^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PU is not modified by combination with single copy of ex⁶⁹⁷.

(Zaessinger et al., 2015)

Mer⁴; ex⁶⁹⁷ double mutants exhibit overgrown wing imaginal discs.

(Maitra et al., 2006)

Mer⁴; ex⁶⁹⁷ double mutant eye-antennal imaginal discs have severely reduced eye primordia with a substantial reduction in or total absence of photoreceptors. In these mutants, photoreceptor development and overall eye field size are severely reduced. The antennal portion is normal or slightly larger than normal and in some cases is duplicated. Apoptosis does not seem to be affected.

Mer⁴; ex⁶⁹⁷ somatic clones in contact with the posterior or lateral margin of the eye fail to produce photoreceptors. Those somatic clones located in the middle of the eye field can produce photoreceptors.

(Maitra et al., 2006)

The broad wing phenotype of ex⁶⁹⁷ homozygotes is dominantly enhanced by Mer¹. Dominantly enhances the head and broad wing phenotypes of Mer³ hemizygotes. ex⁶⁹⁷ dpp^hr56/ex⁶⁹⁷ flies have truncated legs in which the distal tarsal segments are absent (significant leg defects are not seen in either dpp^hr56/+ or ex⁶⁹⁷ single homozygote flies).

(McCartney et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

44248

w^*; P{lacW}ex⁶⁹⁷/SM6a

Notes on Origin

Discoverer

Comments

Dysgenesis induced excisions of the P element can be accompanied by reversion of the mutant phenotype. An allelic series can be defined for ex alleles with respect to viability, eclosion rate and penetrance of ex wing phenotype. Going from most to least severe: ex^e1 >= ex^l2ey > ex^e2 > ex^e6 > ex⁶⁹⁷ > ex¹.

(Boedigheimer and Laughon, 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ex⁶⁹⁷

(Wang et al., 2022, Yang and Choi, 2021, Wang and Baker, 2019, Yee et al., 2019, Anderson et al., 2017, Hirabayashi and Cagan, 2015, Vrabioiu and Struhl, 2015, Zaessinger et al., 2015, Grusche et al., 2011, Hamaratoglu et al., 2006, Hamaratoglu et al., 2006, Maitra et al., 2006, Willecke et al., 2006)

ex⁹

(Hamaratoglu et al., 2006)

ex^Z

(Vrabioiu and Struhl, 2015)

Name Synonyms

Secondary FlyBase IDs

References (18)

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