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General Information
Symbol
Dmel\Cskj1D8
Species
D. melanogaster
Name
l(3)j1D8j1D8
FlyBase ID
FBal0176300
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dCskj1D8, l(3)j1D8
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Consists of 12kb insertion of a FB element, and a crippled P{lacW} element. The insertion also marks the site of a 1.5kb genomic deletion.

Insertion components
P{3'lacW}j1D8
FB{}j1D8
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Cskc04256/Cskj1D8 third instar larvae do not show significant electrophysiological defects (mEPSPs, EPSPs and quantal content similar to wild type).

Wing discs of homozygous third instar larvae show considerable apoptosis.

Mutant larvae die within 6-8 hours after pupariation. The larval body mass of mutants increases up to 180% of the wild-type mass during the wandering stage. The brain ventral ganglion and salivary glands of mutants are enlarged. Pupariation is delayed. The body length of mutant pupae are about 121% the size of wild-type. Imaginal discs of mutant larvae are also larger than wild-type. The larval body mass of Cskj1D8/CskS030003 mutants increases up to 150% of the wild-type mass during the wandering stage. In the rare cases mutants survived to pharate adult stage, the head and eyes are enlarged. The posterior ommatidia are sometimes misaligned, though individual ommatidia are morphologically normal. Mutant eyes contain more ommatidia than wild-type. Rarely the eyes are replaced with duplicated antennae. In addition the wings and legs are severely malformed, the notum is sometimes split, and the head, legs and notum often contain cuticle overgrowths. In Cskj1D8/CskS030003 and homozygous Cskj1D8 mutant developing eye discs, an increase is seen in the number of mitotic cells in the morphogenetic furrow. Mutant pupal retinas contain excess mitotic cells in the interommatidial lattice. Cskj1D8/CskS030003 eye antennal disc cells exhibit a decrease in the proportion of cells in G0-G1 phase, and a consequent increase in cells in G2-M phase when compared to wild-type controls. Homozygous mutant clones in the eye produced severely overgrown eyes. Eyes have more than 1200 ommatidia, compared to about 687 in wild-type. Eyes can become malformed as a result. The extra ommatidia are occasionally inverted in their planar polarity but are otherwise normal in size and morphology. Occasionally mutant clones result in antennal duplication and cuticle overgrowth.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

Cskj1D8, pucE69/puc[+] has visible phenotype, enhanceable by ASPP[+]/ASPP8

Suppressed by
NOT suppressed by
Statement
Reference

Cskj1D8/CskS030003 has lethal | pupal stage phenotype, non-suppressible by Src42Ak10108

Cskj1D8/CskS030003 has lethal | pupal stage phenotype, non-suppressible by chico1

Cskj1D8/CskS030003 has lethal | pupal stage phenotype, non-suppressible by ftG-rv

Cskj1D8/CskS030003 has lethal | pupal stage phenotype, non-suppressible by scb2

Cskj1D8/CskS030003 has lethal | pupal stage phenotype, non-suppressible by shgk03401

Cskj1D8/CskS030003 has lethal | pupal stage phenotype, non-suppressible by CadNM12

Cskj1D8/CskS030003 has lethal | pupal stage phenotype, non-suppressible by Egfrf2

Cskj1D8/CskS030003 has lethal | pupal stage phenotype, non-suppressible by JraIA109

Cskj1D8/CskS030003 has lethal | pupal stage phenotype, non-suppressible by Sos34Ea-6

Enhancer of
Statement
Reference

Cskj1D8/Csk[+] is an enhancer of visible phenotype of RetMEN2B.GMR

Cskj1D8/Csk[+] is an enhancer of visible phenotype of RetGMR.PR

NOT Enhancer of
Statement
Reference

Cskj1D8/Csk[+] is a non-enhancer of visible | adult stage phenotype of MagiUAS.GFP, Scer\GAL4GMR.PU

Cskj1D8/Csk[+] is a non-enhancer of visible | dominant phenotype of EgfrE1

Cskj1D8/Csk[+] is a non-enhancer of visible phenotype of RetMEN2A.GMR

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

Cskj1D8/Csk[+] is a non-suppressor of visible | adult stage phenotype of MagiUAS.GFP, Scer\GAL4GMR.PU

Cskj1D8/Csk[+] is a non-suppressor of visible phenotype of RetMEN2A.GMR

Cskj1D8/Csk[+] is a non-suppressor of visible | dominant phenotype of EgfrE1

Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Cskj1D8, pucE69/puc[+] has wing phenotype, enhanceable by ASPP[+]/ASPP8

Cskj1D8 has eye | somatic clone phenotype, enhanceable by Stat92Ej6C8

Suppressed by
Statement
Reference

ASPPd/ASPP8, Cskj1D8 has wing phenotype, suppressible by ASPPUbi-p63E.GFP

ASPP8, Cskj1D8/Csk[+] has wing phenotype, suppressible by Btk29A[+]/Btk29Ak00206

ASPP8, Cskj1D8/Csk[+] has wing disc phenotype, suppressible by Btk29A[+]/Btk29Ak00206

Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference

Cskj1D8/Csk[+] is a non-enhancer of eye | adult stage phenotype of MagiUAS.GFP, Scer\GAL4GMR.PU

Cskj1D8/Csk[+] is a non-enhancer of eye phenotype of EgfrE1

Cskj1D8/Csk[+] is a non-enhancer of eye phenotype of RetMEN2A.GMR

Suppressor of
Statement
Reference

Cskj1D8 is a suppressor of ommatidium phenotype of wtsGMR.PS

Cskj1D8 is a suppressor of eye phenotype of wtsGMR.PS

NOT Suppressor of
Statement
Reference

Cskj1D8/Csk[+] is a non-suppressor of eye | adult stage phenotype of MagiUAS.GFP, Scer\GAL4GMR.PU

Cskj1D8/Csk[+] is a non-suppressor of eye phenotype of RetMEN2A.GMR

Cskj1D8/Csk[+] is a non-suppressor of eye phenotype of EgfrE1

Other
Additional Comments
Genetic Interactions
Statement
Reference

ASPPd/ASPP8, Cskj1D8/+ adults very frequently show wing notches, which are never observed in controls; this phenotype is fully rescued by ASPPUbi-p63E.GFP, but only partially by ASPPFA.Ubi-p63E.GFP.

Third instar larval GluRIIASP16/GluRIIASP16;Cskc04256/Cskj1D8 double mutants show significant decreases in mEPSP (decreasing quantal size) but do not show significant increases in quantal content (as seen in GluRIIASP16/GluRIIASP16 mutants alone) at the NMJ. Additional presence Cskj1D8/+ does not alter differences in quantal size and content seen in GluRIIASP16/GluRIIASP16 larvae. GluRIIASP16/GluRIIASP16 cacS/+ Cskj1D8/+ third instar larvae show significant decreases in mEPSP and quantal content at the NMJ.

GluRIIASP16/GluRIIASP16 Cskc04256/Cskj1D8 Src64Bko/+ third instar larvae show significant decreases in mEPSP (decreased quantal size) partially restores significant compensatory homeostatic increases in quantal content (unlike GluRIIASP16/GluRIIASP16 Cskc04256/Cskj1D8 double mutants, which do not show significant increases in quantal content) at the NMJ.

GluRIIASP16/GluRIIASP16;Cskc04256/Cskj1D8 third instar larvae show significant decreases in mEPSP (decreasing quantal size) but do not show significant compensatory homeostatic increases in quantal content (as seen in GluRIIASP16/GluRIIASP16 mutants alone) at the NMJ; additional expression of CskScer\UAS.T:Avic\GFP-YFP driven by Scer\GAL4nau.PM or Scer\GAL4VGlut-OK371 restores phenotypes to those seen in GluRIIASP16/GluRIIASP16 mutants alone.

GluRIIASP16/GluRIIASP16;Cskc04256/Cskj1D8 third instar larvae show significant decreases in mEPSP (decreasing quantal size) but do not show significant compensatory homeostatic increases in quantal content (as seen in GluRIIASP16/GluRIIASP16 mutants alone) at the NMJ; additional presence of Fas2e76 restores phenotypes to those seen in GluRIIASP16/GluRIIASP16 mutants alone. GluRIIASP16/GluRIIASP16;Cskc04256/Cskj1D8 Fas2e76 third instar larvae also show a small significant increase in bouton number per muscle area (segment A2, synapse 6/7).

The rough eye phenotype observed in adults expressing MagiScer\UAS.T:Avic\GFP under the control of Scer\GAL4GMR.PU is not modified by combination with single copy of Cskj1D8.

The tumor overgrowth of somatic clones (induced specifically in the eye disc) expressing Ras85DV12.Scer\UAS under the control of Scer\GAL4Act.PU is suppressed when the clones are also homozygous for Cskj1D8.

A Cskj1D8/+ background enhances the visual system degeneration seen in Scer\GAL4GMR.PF, Ppt1Scer\UAS.cKa flies.

68% of ASPPd ; Cskj1D8/+ flies have notched wings.

ASPP8/ASPP8 Cskj1D8/+ adults show a notched wing phenotype. Wing discs of ASPP8/ASPP8 Cskj1D8/+ third instar larvae show considerable apoptosis. Neither phenotype is seen in ASPP8/ASPP8 or Cskj1D8/+ single mutants.

Btk29Ak00206/+ largely suppresses the ectopic apoptosis in wing discs and notched wing phenotype seen in ASPP8/ASPP8 Cskj1D8/+ animals.

pucE69/+ Cskj1D8/+ double heterozygotes have a notched wing phenotype, and third larval instar wing discs show ectopic apoptosis within the wing pouch.

The ectopic apoptosis seen in the wing discs of pucE69/+ Cskj1D8/+ double heterozygotes is enhanced if they are also heterozygous for ASPP8, resulting in adults with a severely reduced wing size. These adults also have leg deformities.

Cskj1D8, Stat92Ej6C8 mutant eyes are smaller than eyes mutant for Cskj1D8 or Stat92Ej6C8 alone. In addition, adult eyes are frequently fragmented, with scars and/or patches of eye tissue separated by patches of cuticle. Stat92E06346. Cskj1D8 mutant larval eye-antennal discs frequently show reduced sizes relative to the single mutants. Mutants also frequently show a reduction or absence of developing antennal tissues. Mutant larval eye tissue also contains many apoptotic cells.

Xenogenetic Interactions
Statement
Reference

Cskj1D8 dominantly suppresses the rough eye phenotype caused by expression of Zzzz\CTGi480.Scer\UAS.cGa under the control of Scer\GAL4sev.PU.

Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (12)
References (18)