Df(3R)BSC469 was isolated as a FLP recombinase-induced recombination
event involving PBac{WH}CG17187[f03477] and P{XP}d03525. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; PBac{WH}CG17187[f03477]/P{XP}d03525 males. The males were 
heat shocked as larvae as described in Parks et al., Nature Genetics 
36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding 
and succeeding generations maintained the original genetic background 
of the Exelixis insertion stocks (Thibault et al., Nature Genetics 
36: 283-287, 2004; FBrf0175002). The recombination event generated 
the genetic element P+PBac{XP5.WH5}BSC469 from the segment of 
PBac{WH}CG17187[f03477] to the left of its FRT site and the segment 
of P{XP}d03525 to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(3R)BSC469 predicted from the 
Release 5 genomic coordinates of the transposable element insertions 
sites are 86D8;87A2. Df(3R)BSC469 failed to complement pros[10419] 
and Csk[j1D8].