FlyBase Allele Report: Dmel\Csk[UAS.YFP]

General Information

Symbol

Dmel\Csk^UAS.YFP

Species

D. melanogaster

Name

FlyBase ID

FBal0319314

Feature type

allele

Associated gene

Dmel\Csk

Associated Insertion(s)

Carried in Construct

P{UAS-Csk.YFP}

Key Links

Transgenic product class

cDNA

(Spring et al., 2016)

wild_type

(Spring et al., 2016)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Spring et al., 2016)

wild_type

(Spring et al., 2016)

Mutagen

in vitro construct

(Spring et al., 2016)

Progenitor genotype

Carried in construct

P{UAS-Csk.YFP}

Cytology

Description

UASt regulatory sequences drive expression of the PH isoform of Csk (derived from cDNA clone LP09923) which is tagged at the C-terminal end with YFP.

(Spring et al., 2016)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Csk

Tagged with

YFP

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^C57, Scer\GAL4^elav-C155, Scer\GAL4^sca.PC

(Spring et al., 2016)

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^C57, Scer\GAL4^elav-C155, Scer\GAL4^sca.PC

(Spring et al., 2016)

Detailed Description

Statement

Reference

Expression of Csk^{Scer\UAS.T:Avic\GFP-YFP} driven simultaneously by Scer\GAL4^elav-C155, Scer\GAL4^sca.PC and Scer\GAL4^C57 significantly increases miniature excitatory postsynaptic potential (mEPSP) amplitudes and significantly decreases evoked postsynaptic potentiation (EPSP) amplitude and quantal content at the neuromuscular junction (NMJ) of third instar larvae, compared to controls.

(Spring et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Csk^UAS.YFP, Fas2^EP1462, GluRIIA^SP16, Scer\GAL4^C57 has abnormal neurophysiology | third instar larval stage phenotype

(Spring et al., 2016)

Csk^UAS.YFP, Csk^j1D8/Csk^c04256, GluRIIA^SP16, Scer\GAL4^nau.PM has abnormal neurophysiology | third instar larval stage phenotype

(Spring et al., 2016)

Phenotype Manifest In

Other

Statement

Reference

Csk^UAS.YFP, Fas2^EP1462, GluRIIA^SP16, Scer\GAL4^C57 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Spring et al., 2016)

Csk^UAS.YFP, Csk^j1D8/Csk^c04256, GluRIIA^SP16, Scer\GAL4^nau.PM has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Spring et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

GluRIIA^SP16/GluRIIA^SP16;Csk^c04256/Csk^j1D8 third instar larvae show significant decreases in mEPSP (decreasing quantal size) but do not show significant compensatory homeostatic increases in quantal content (as seen in GluRIIA^SP16/GluRIIA^SP16 mutants alone) at the NMJ; additional expression of Csk^{Scer\UAS.T:Avic\GFP-YFP} driven by Scer\GAL4^nau.PM or Scer\GAL4^VGlut-OK371 restores phenotypes to those seen in GluRIIA^SP16/GluRIIA^SP16 mutants alone.

Third instar larvae with co-expression of GluRIIC^{dsRNA.Scer\UAS.cBa} and Csk^{Scer\UAS.T:Avic\GFP-YFP} driven simultaneously by Scer\GAL4^elav-C155, Scer\GAL4^sca.PC and Scer\GAL4^C57 show significant decreases in mEPSP (decreasing quantal size) and show significant compensatory homeostatic increases in quantal content (as seen with expression of GluRIIC^{dsRNA.Scer\UAS.cBa} alone) at the NMJ.

Third instar GluRIIA^SP16/GluRIIA^SP16 larvae with co-expression of Fas2^EP1462 and Csk^{Scer\UAS.T:Avic\GFP-YFP} driven by Scer\GAL4^C57 show significant decreases in mEPSP (decreasing quantal size) and show complete impairment of compensatory homeostatic increases in quantal content (compared to significant quantal content increases seen in GluRIIA^SP16/GluRIIA^SP16 mutants alone) at the NMJ.

(Spring et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Csk^{Scer\UAS.T:Avic\GFP-YFP}

Csk^UAS.YFP

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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