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General Information
Symbol
Dmel\Stat92Ej6C8
Species
D. melanogaster
Name
FlyBase ID
FBal0010984
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
l(3)J6C8
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Insertion components
P{lacW}Stat92Ej6C8
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

24% of Stat92Ej6C8/Df(3R)H-B79 animals die during embryogenesis and the remainder die during the first larval instar.

Marked homozygous somatic cyst progenitor cell clones in the testis are extremely unstable compared to wild-type control clones.

Somatic cyst progenitor cells (CPCs) and germline stem cells are completely lost and the hub moves to the inside in most testes after 4 days at 29[o]C in Stat92EF/Stat92Ej6C8 males (raised at 22[o]C). 70% of the testes retain some somatic cyst cells. Marker analysis indicates that the CPCs differentiate into daughter somatic cyst cells in the mutant testes at 29[o]C. When the flies are shifted from 29[o]C back to 18[o]C for 3 days, marker analysis suggests that the daughter somatic cyst cells are able to de-differentiate back into CPCs.

After shifting to the restrictive temperature of 29[o]C, germline stem cells, spermatogonia and spermatocytes are gradually lost over time in Stat92EF/Stat92Ej6C8 testes. After 4 days at 29[o]C, germline stem cells are completely lost and only a fraction of testes retain spermatogonia. After 7 days at 29[o]C, germline stem cells, spermatogonia and spermatocytes are completely lost in all Stat92EF/Stat92Ej6C8 testes.

Stat92Ej6C8 homozygous female germ line clones develop normally. Early oogenesis is unaffected when follicle stem cells are homozygous for Stat92Ej6C8 (as somatic clones) but cysts containing mutant follicle cells frerquently do not become encapsulated. In contarst, when escort cells are homozygous for Stat92Ej6C8 (as somatic clones), germaria become distorted close to the mutant cells, lack normal patterns of developing cysts, and are suurounded by an expanded muscle sheath and associated basement membrane. Numbers of germline stem cells are reduced in these germaria and mislocalised spectrosomes.

When homozygous mutant eyes are made using somatic clones, the resultant eyes are mostly normal showing a slight reduction in size, some misaligned ommatidia and infrequently, missing antennal structures.

Follicle cell clones of Stat92Ej6C8 cause a cell-autonomous loss-of-stalk fate. When such Stat92Ej6C8 clones prevent the formation of the anterior stalk of an cyst, this chamber fuses with the adjacent anterior cyst. The oocyte of the anterior egg chamber is then mispositioned. In contrast, when all of the epithelial follicle cells and the polar cells are Stat92Ej6C8 clones, but the stalk cells are wild-type, the oocyte is positioned correctly.

Females with homozygous clones in the follicle cells show egg chamber fusions.

Homozygous germ line stem cells are frequently detected in the testis two days after clone induction. However, the percentage of testes containing germ line stem cell clones decreases over time and they are absent by 5 days after clone induction.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

Stat92Ej6C8/Stat92E[+] is a suppressor of visible phenotype of upd1GMR.PB

Stat92Ej6C8/Stat92E[+] is a suppressor of size defective | adult stage phenotype of upd1GMR.PB

NOT Suppressor of
Phenotype Manifest In
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

Stat92EF/Stat92Ej6C8 has spermatogonium | heat sensitive phenotype, non-suppressible by Scer\GAL4nos.PG/upd1UAS.cCa

Stat92EF/Stat92Ej6C8 has male germline stem cell | heat sensitive phenotype, non-suppressible by Scer\GAL4nos.PG/upd1UAS.cCa

Enhancer of
Statement
Reference

Stat92Ej6C8 is an enhancer of eye | somatic clone phenotype of Cskj1D8

NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

Stat92Ej6C8 is a suppressor of eye phenotype of upd1GMR.PB

Stat92Ej6C8/Stat92E[+] is a suppressor of eye phenotype of upd1GMR.PB

NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

Stat92EF/Stat92Ej6C8 males expressing Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4C587 show complete loss of somatic cyst progenitor cells, somatic cyst cells, germline stem cells, spermatogonia and spermatocytes after 2 days at 29[o]C.

Hyperplasia in adult Malpighian tubule clones expressing Ras85DV12.Scer\UAS (using the MARCM system, under the control of Scer\GAL80αTub84B.PL and Scer\GAL4Scer\FRT.Act5C) is not suppressed by making them also mutant for Stat92Ej6C8.

Expression of BHDScer\UAS.cSa under the control of Scer\GAL4nos.PG significantly slows down the loss of germ cell phenotype that is seen in Stat92EF/Stat92Ej6C8 testes at 29[o]C. After 4 days at 29[o]C, 26% of the double mutant testes contain one or more germline stem cells and most of the testes retain spermatogonia. After 7 days at 29[o]C, 8% of the double mutant testes have one or more germline stem cells and 64% retain spermatogonia.

Expression of upd1Scer\UAS.cCa under the control of Scer\GAL4nos.PG does not suppress the loss of germ cell phenotype that is seen in Stat92EF/Stat92Ej6C8 testes at 29[o]C.

Expression of dppScer\UAS.cUa under the control of Scer\GAL4nos.PG significantly slows down the loss of germ cell phenotype that is seen in Stat92EF/Stat92Ej6C8 testes at 29[o]C. After 4 days at 29[o]C, 41% of the double mutant testes contain one or more germline stem cells and all of the testes retain spermatogonia. After 7 days at 29[o]C, 17% of the double mutant testes have one or more germline stem cells and 88% retain spermatogonia.

Heterozygosity for Stat92Ej6C8 fails to suppress the large eye phenotype of both Scer\GAL4ey.PH > SerScer\UAS.cSa and Scer\GAL4ey.PH > DlScer\UAS.cHa flies.

The enlarged eye phenotype seen in flies carrying one copy of upd1GMR.PB is strongly suppressed by one copy of Stat92Ej6C8.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

Expression of Stat92EScer\UAS.cSa under the control of Scer\GAL4C587 rescues the loss of somatic cyst progenitor cells that is seen after 4 days at 29[o]C in the testes of Stat92EF/Stat92Ej6C8 males (raised at 22[o]C). The rescued animals also have wild-type somatic cyst cells and most germline stem cells are also restored.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

L. and Y. Jan.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (17)