FlyBase Allele Report: Dmel\upd1[UAS.cCa]

General Information

Symbol

Dmel\upd1^UAS.cCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Chen

FlyBase ID

FBal0134042

Feature type

allele

Associated gene

Dmel\upd1

Associated Insertion(s)

Carried in Construct

P{UAS-upd1.C}

Also Known As

UAS-Upd

Key Links

Transgenic product class

cDNA

(Chen et al., 2002)

wild_type

(Chen et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Chen et al., 2002)

wild_type

(Chen et al., 2002)

Mutagen

in vitro construct

(Chen et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-upd1.C}

Cytology

Description

UASt regulatory sequences drive expression of a upd1 cDNA.

(Chen et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

upd1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

(Wang et al., 2006)

increased cell number | oogenesis, with Scer\GAL4^{VP16.nanos.UTR}

(Wang et al., 2006)

increased occurrence of cell division | adult stage, with Scer\GAL4^esg.PU

(Zhao et al., 2022)

increased occurrence of cell division | adult stage, with Scer\GAL4^NP0001

(Wang et al., 2015)

increased size | adult stage, with Scer\GAL4^ey.PU

(Harsh et al., 2020)

visible, with Scer\GAL4^GMR.PF

(Baeg et al., 2005, Chen et al., 2002)

visible | adult stage, with Scer\GAL4^ey.PU

(Harsh et al., 2020)

Phenotype Manifest In

adult gut, with Scer\GAL4^NP0001

(Wang et al., 2015)

adult posterior midgut epithelium, with Scer\GAL4^esg.PU

(Zhao et al., 2022)

border follicle cell | ectopic | somatic clone, with Scer\GAL4^Act5C.PI

(Silver et al., 2005)

cuticle & adult external head, with Scer\GAL4^ey.PH

(Ekas et al., 2006)

eye, with Scer\GAL4^ey.PH

(Ekas et al., 2006)

eye, with Scer\GAL4^ey.PU

(Harsh et al., 2020)

eye, with Scer\GAL4^GMR.PF

(Baeg et al., 2005, Chen et al., 2003, Chen et al., 2002)

eye disc, with Scer\GAL4^GMR.PF

(Chen et al., 2003)

eye disc morphogenetic furrow, with Scer\GAL4^ey.PH

(Ekas et al., 2006)

germline stem cell | increased number, with Scer\GAL4^{VP16.nanos.UTR}

(Wang et al., 2006)

male germline stem cell | increased number, with Scer\GAL4^nanos.PG

(Singh et al., 2006)

ommatidium, with Scer\GAL4^GMR.PF

(Chen et al., 2003)

ommatidium | increased number, with Scer\GAL4^GMR.PF

(Chen et al., 2002)

primary gonial cell | increased number, with Scer\GAL4^nanos.PG

(Singh et al., 2006)

stalk follicle cell, with Scer\GAL4^neur-P72

(Assa-Kunik et al., 2007)

testis, with Scer\GAL4^nanos.PG

(Singh et al., 2006)

testis, with Scer\GAL4^{VP16.nanos.UTR}

(Wang et al., 2006)

Detailed Description

Statement

Reference

Adulthood-only expression of upd1^UAS.cCa under the control of Scer\GAL4^esg.PU leads to an increase in mitotic index in the gut.

(Zhao et al., 2022)

Expression of upd1^UAS.cCa under the control of Scer\GAL4^ey.PU leads to an increase in the size of eye.

(Harsh et al., 2020)

Expression of upd1^Scer\UAS.cCa under the control of Scer\GAL4^{Myo31DF-NP0001} (using tub-gal80[ts] to limit the time of expression) increases cell proliferation in the adult gut.

(Wang et al., 2015)

Expression of upd1^Scer\UAS.cCa under the control of Scer\GAL4^neur-P72 results in abnormally long stalks between egg chambers.

(Assa-Kunik et al., 2007)

Adults that express upd1^Scer\UAS.cCa under the control of Scer\GAL4^ey.PH show overgrowth of the eye field and replacement of the lateral head cuticle by photoreceptors, causing a reduction in the distance between the eyes. Eye discs of this genotype show precocious furrow formation from the lateral margins.

(Ekas et al., 2006)

Expression of upd1^Scer\UAS.cCa under the control of Scer\GAL4^nos.PG results in ectopic cells with germline stem cell and gonialblast features in the testis and ballooning of the whole testis.

(Singh et al., 2006)

The testes of upd1^Scer\UAS.cCa; Scer\GAL4^{nos.UTR.T:Hsim\VP16} animals are greatly enlarged and filled with many germline stem cells (identifiable by their unbranched fusomes).

(Wang et al., 2006)

Expression of upd1^Scer\UAS.cCa, under the control of Scer\GAL4^GMR.PF results in the dramatic overgrowth and deformation of the adult eye.

(Baeg et al., 2005)

Anterior follicle cell clones that express upd1^Scer\UAS.cCa, driven by Scer\GAL4^Act5C.PI, recruit ectopic border cells. These clones recruit an average of 1.1 border cell per upd1^Scer\UAS.cCa-expressing cluster, while polar cells recruit four to eight border cells to surround them.

(Silver et al., 2005)

Expression of upd1^Scer\UAS.cCa suppresses the border cell migration defect caused by expression of Rac1^N17.Scer\UAS under the control of Scer\GAL4^slbo.2.6.

(Silver, 2005)

upd1^Scer\UAS.cCa; Scer\GAL4^GMR.PF animals have overgrowth of the eye (and of the eye disc in third instar larvae) due to an increase in the number of ommatidia.

(Chen et al., 2003)

Expression of upd1^Scer\UAS.cCa under the control of Scer\GAL4^GMR.PF results in a dramatic overgrowth of the eye, due to an increase in the number of ommatidia; the eyes contain an average of 978 +/- 10 ommatidia compared to 745 +/- 7 in wild-type flies. Most of the ommatidia contain the normal number of photoreceptor cells and have regular cell size (although a few have one extra R7 photoreceptor cell). The ommatidia look more crowded than normal and have irregular space and arrangement, with several large vacuoles in the ommatidial lattice.

(Chen et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, upd1^UAS.cCa has visible phenotype, enhanceable by Df(3L)ED4238/+

(Baeg et al., 2005)

NOT Enhanced by

Statement

Reference

Scer\GAL4^ey.PU, upd1^UAS.cCa has increased size | adult stage phenotype, non-enhanceable by ZIKV\NS4A^UAS.Tag:FLAG/Scer\GAL4^ey.PU

(Harsh et al., 2020)

Scer\GAL4^ey.PU, upd1^UAS.cCa has visible | adult stage phenotype, non-enhanceable by ZIKV\NS4A^UAS.Tag:FLAG/Scer\GAL4^ey.PU

(Harsh et al., 2020)

Suppressed by

Statement

Reference

Scer\GAL4^esg.PU, upd1^UAS.cCa has increased occurrence of cell division | adult stage phenotype, suppressible by dind^{RNAi.UAS.RNAi}/Scer\GAL4^esg.PU

(Zhao et al., 2022)

Scer\GAL4^esg.PU, upd1^UAS.cCa has increased occurrence of cell division | adult stage phenotype, suppressible by dind^NIG.7705R/Scer\GAL4^esg.PU

(Zhao et al., 2022)

Scer\GAL4^esg.PU, upd1^UAS.cCa has increased occurrence of cell division | adult stage phenotype, suppressible by Phb1^HMS00399, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Scer\GAL4^esg.PU, upd1^UAS.cCa has increased occurrence of cell division | adult stage phenotype, suppressible by Phb1^HMS00702, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Scer\GAL4^esg.PU, upd1^UAS.cCa has increased occurrence of cell division | adult stage phenotype, suppressible by Phb2^HMS02001, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Scer\GAL4^esg.PU, upd1^UAS.cCa has increased occurrence of cell division | adult stage phenotype, suppressible by Phb2^GD8538, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Scer\GAL4^GMR.PF, upd1^UAS.cCa has visible phenotype, suppressible by dome[+]/dome^G0264

(Chen et al., 2002)

Scer\GAL4^GMR.PF, upd1^UAS.cCa has visible phenotype, suppressible by dome[+]/dome^G0367

(Chen et al., 2002)

Scer\GAL4^GMR.PF, upd1^UAS.cCa has visible phenotype, suppressible by dome^G0441/dome[+]

(Chen et al., 2002)

Scer\GAL4^GMR.PF, upd1^UAS.cCa has visible phenotype, suppressible by hop²/hop[+]

(Chen et al., 2002)

NOT suppressed by

Statement

Reference

Scer\GAL4^ey.PU, upd1^UAS.cCa has increased size | adult stage phenotype, non-suppressible by ZIKV\NS4A^UAS.Tag:FLAG/Scer\GAL4^ey.PU

(Harsh et al., 2020)

Scer\GAL4^ey.PU, upd1^UAS.cCa has visible | adult stage phenotype, non-suppressible by ZIKV\NS4A^UAS.Tag:FLAG/Scer\GAL4^ey.PU

(Harsh et al., 2020)

Suppressor of

Statement

Reference

upd1^UAS.cCa/Scer\GAL4^esg.PU is a suppressor of increased cell number | adult stage phenotype of Scer\GAL4^esg.PU, hop^Tum.UAS

(Zhao et al., 2022)

Other

Statement

Reference

Df(3L)ED4238/+, Scer\GAL4^GMR.PF, upd1^UAS.cCa has partially lethal phenotype

(Baeg et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, upd1^UAS.cCa has eye phenotype, enhanceable by Df(3L)ED4238/+

(Baeg et al., 2005)

Scer\GAL4^GMR.PF, upd1^UAS.cCa has eye disc phenotype, enhanceable by CycD^UAS.cDa/Cdk4^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Chen et al., 2003)

Scer\GAL4^GMR.PF, upd1^UAS.cCa has ommatidium phenotype, enhanceable by CycD^UAS.cDa/Cdk4^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Chen et al., 2003)

Scer\GAL4^GMR.PF, upd1^UAS.cCa has eye phenotype, enhanceable by CycD^UAS.cDa/Cdk4^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Chen et al., 2003)

NOT Enhanced by

Statement

Reference

Scer\GAL4^ey.PU, upd1^UAS.cCa has eye phenotype, non-enhanceable by ZIKV\NS4A^UAS.Tag:FLAG/Scer\GAL4^ey.PU

(Harsh et al., 2020)

Suppressed by

Statement

Reference

Scer\GAL4^esg.PU, upd1^UAS.cCa has adult posterior midgut epithelium phenotype, suppressible by dind^{RNAi.UAS.RNAi}/Scer\GAL4^esg.PU

(Zhao et al., 2022)

Scer\GAL4^esg.PU, upd1^UAS.cCa has adult posterior midgut epithelium phenotype, suppressible by dind^NIG.7705R/Scer\GAL4^esg.PU

(Zhao et al., 2022)

Scer\GAL4^esg.PU, upd1^UAS.cCa has adult posterior midgut epithelium phenotype, suppressible by Phb1^HMS00399, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Scer\GAL4^esg.PU, upd1^UAS.cCa has adult posterior midgut epithelium phenotype, suppressible by Phb2^HMS02001, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Scer\GAL4^esg.PU, upd1^UAS.cCa has adult posterior midgut epithelium phenotype, suppressible by Phb2^GD8538, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Scer\GAL4^esg.PU, upd1^UAS.cCa has adult posterior midgut epithelium phenotype, suppressible by Phb1^HMS00702, Scer\GAL4^esg.PU

(Zhao et al., 2022)

Scer\GAL4^{VP16.nanos.UTR}, upd1^UAS.cCa has phenotype, suppressible by Socs36E^EY06665

(Singh et al., 2010)

Scer\GAL4^nanos.PG, upd1^UAS.cCa has testis phenotype, suppressible | partially by BHD^RNAi.Sym.UAS, Scer\GAL4^nanos.PG

(Singh et al., 2006)

Scer\GAL4^nanos.PG, upd1^UAS.cCa has male germline stem cell | increased number phenotype, suppressible | partially by BHD^RNAi.Sym.UAS, Scer\GAL4^nanos.PG

(Singh et al., 2006)

Scer\GAL4^nanos.PG, upd1^UAS.cCa has testis phenotype, suppressible | partially by Df(3L)Exel6111/+

(Singh et al., 2006)

Scer\GAL4^GMR.PF, upd1^UAS.cCa has eye phenotype, suppressible by dome[+]/dome^G0264

(Chen et al., 2002)

Scer\GAL4^GMR.PF, upd1^UAS.cCa has eye phenotype, suppressible by dome[+]/dome^G0367

(Chen et al., 2002)

Scer\GAL4^GMR.PF, upd1^UAS.cCa has eye phenotype, suppressible by dome^G0441/dome[+]

(Chen et al., 2002)

Scer\GAL4^GMR.PF, upd1^UAS.cCa has eye phenotype, suppressible by hop²/hop[+]

(Chen et al., 2002)

Scer\GAL4^GMR.PF, upd1^UAS.cCa has ommatidium | increased number phenotype, suppressible by dome[+]/dome^G0264

(Chen et al., 2002)

Scer\GAL4^GMR.PF, upd1^UAS.cCa has ommatidium | increased number phenotype, suppressible by dome[+]/dome^G0367

(Chen et al., 2002)

Scer\GAL4^GMR.PF, upd1^UAS.cCa has ommatidium | increased number phenotype, suppressible by dome^G0441/dome[+]

(Chen et al., 2002)

Scer\GAL4^GMR.PF, upd1^UAS.cCa has ommatidium | increased number phenotype, suppressible by hop²/hop[+]

(Chen et al., 2002)

NOT suppressed by

Statement

Reference

Scer\GAL4^ey.PU, upd1^UAS.cCa has eye phenotype, non-suppressible by ZIKV\NS4A^UAS.Tag:FLAG/Scer\GAL4^ey.PU

(Harsh et al., 2020)

Scer\GAL4^nanos.PG, upd1^UAS.cCa has male germline stem cell | increased number phenotype, non-suppressible by Scer\GAL4^nanos.PG/Stat92E^UAS.cSa

(Singh et al., 2006)

Suppressor of

Statement

Reference

upd1^UAS.cCa/Scer\GAL4^esg.PU is a suppressor of adult posterior midgut epithelium phenotype of Scer\GAL4^esg.PU, hop^Tum.UAS

(Zhao et al., 2022)

upd1^UAS.cCa, Scer\GAL4^slbo.2.6 is a suppressor of border follicle cell phenotype of Rac1^N17.UAS, Scer\GAL4^slbo.2.6

(Silver, 2005)

NOT Suppressor of

Statement

Reference

Scer\GAL4^nanos.PG/upd1^UAS.cCa is a non-suppressor of spermatogonium | heat sensitive phenotype of Stat92E^F/Stat92E^j6C8

(Singh et al., 2006)

Scer\GAL4^nanos.PG/upd1^UAS.cCa is a non-suppressor of male germline stem cell | heat sensitive phenotype of Stat92E^F/Stat92E^j6C8

(Singh et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of BHD^{dsRNA.Sym.Scer\UAS} significantly suppresses the testis phenotype caused by expression of upd1^Scer\UAS.cCa under the control of Scer\GAL4^nos.PG; the number of germline stem cells is significantly reduced, and the overall shape of the testis is closer to that of wild type.

Df(3L)Exel6111/+ significantly suppresses the testis phenotype caused by expression of upd1^Scer\UAS.cCa under the control of Scer\GAL4^nos.PG.

Co-expression of Stat92E^Scer\UAS.cSa has no effect on the germline stem cell expansion phenotype seen in the testis of males expressing upd1^Scer\UAS.cCa under the control of Scer\GAL4^nos.PG.

Expression of upd1^Scer\UAS.cCa under the control of Scer\GAL4^nos.PG does not suppress the loss of germ cell phenotype that is seen in Stat92E^F/Stat92E^j6C8 testes at 29[o]C.

(Singh et al., 2006)

PDZ-GEF³/PDZ-GEF⁴ has no effect on the male germline stem cell over-proliferation phenotype due to upd1^Scer\UAS.cCa; Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Wang et al., 2006)

Df(3L)ED4238/+ enhances the eye defects caused by expression of upd1^Scer\UAS.cCa, under the control of Scer\GAL4^GMR.PF.

(Baeg et al., 2005)

Expression of os^Scer\UAS.cCa suppresses the border cell migration defect caused by expression of Rac1^N17.Scer\UAS under the control of Scer\GAL4^slbo.2.6.

(Silver, 2005)

The eye and eye disc overgrowth phenotypes seen in upd1^Scer\UAS.cCa; Scer\GAL4^GMR.PF flies, are dramatically enhanced by CycD^Scer\UAS.cDa with Cdk4^{Scer\UAS.T:Hsap\MYC} : All ommatidia are dramatically enlarged in these eyes, and the entire eye bulges out of the head. The increase in ommatidial numbers seen in upd1^Scer\UAS.cCa; Scer\GAL4^GMR.PF eye discs is also enhanced. The block in photoreceptor differentiation seen in third instar CycD^Scer\UAS.cDa; Cdk4^{Scer\UAS.T:Hsap\MYC}; Scer\GAL4^GMR.PF eye discs, is suppressed by upd1^Scer\UAS.cCa. However, the similar phenotype seen in third instar Cdk2^{Scer\UAS.T:Hsap\MYC}; CycE^Scer\UAS.cLa; Scer\GAL4^GMR.PF eye discs is largely unaffected by upd1^Scer\UAS.cCa.

(Chen et al., 2003)

The extra number of ommatidia caused by expression of upd1^Scer\UAS.cCa under the control of Scer\GAL4^GMR.PF is partially suppressed by hop²/+; the flies have an average of 854 +/- 9 ommatidia. The extra number of ommatidia caused by expression of upd1^Scer\UAS.cCa under the control of Scer\GAL4^GMR.PF is partially suppressed by dome^G0441/+; the flies have an average of 813 +/- 10 ommatidia.

(Chen et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

os^Scer\UAS.cCa

upd1^Scer\UAS.cCa

upd1^UAS.cCa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Chen

(Chen et al., 2002)

Secondary FlyBase IDs

References (20)

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