FB2022_02, released March 29, 2022

Allele: Dmel\hopTum.UAS

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General Information
Symbol
Dmel\hopTum.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0043128
Feature type
allele
Associated gene
Dmel\hop
Associated Insertion(s)
Carried in Construct
P{UAS-hop.Tum}
Also Known As
UAS-hopTuml, UAS-hopTUM, UAS-hopTum-l, HopTuml
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - minigene
(Harrison et al., 1995)
in vitro construct - regulatory fusion
(Harrison et al., 1995)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{UAS-hop.Tum}
      Cytology
      Nature of the lesion
      Statement
      Reference

      UASt regulatory sequences drive expression of a hop cDNA fragment containing the entire coding region with the hopTum mutation.

      (Harrison et al., 1995)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UASt
      Encoded product / tool
      hop
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  disease of cellular proliferation
      CEA
      (Harrison et al., 1995)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      adult gut, with Scer\GAL4NP0001

      (Wang et al., 2015)

      border follicle cell, with Scer\GAL4c306

      (Silver et al., 2005)

      border follicle cell | ectopic, with Scer\GAL4c306

      (Silver and Montell, 2001)

      border follicle cell | ectopic, with Scer\GAL4slbo.2.6

      (Silver and Montell, 2001)

      cyst progenitor cell | increased number, with Scer\GAL4A3.eya

      (Leatherman and DiNardo, 2008)

      embryonic/larval hemocyte | increased number, with Scer\GAL4stop.Hml

      (Sinenko and Mathey-Prevot, 2004)

      embryonic/larval plasmatocyte | increased number, with Scer\GAL4Cg.PA

      (Asha et al., 2003)

      embryonic hindgut, with Scer\GAL4byn-Gal4

      (Wells et al., 2013, Johansen et al., 2003)

      eye, with Scer\GAL4elav.PLu

      (Bach et al., 2003)

      eye, with Scer\GAL4ey.PH

      (Bach et al., 2003)

      eye, with Scer\GAL4ey.PU

      (Harsh et al., 2020, Karsten et al., 2006)

      eye, with Scer\GAL4GMR.PF

      (Bach et al., 2003)

      germarium, with Scer\GAL4C587

      (Liu et al., 2015)

      germarium, with Scer\GAL4GMR25A11

      (Liu et al., 2015)

      lamellocyte, with Scer\GAL4He.PZ

      (Zettervall et al., 2004)

      lamellocyte | increased number, with Scer\GAL4Cg.PA

      (Asha et al., 2003)

      larval outer optic anlage, with Scer\GAL4C855a

      (Richter et al., 2011)

      male germline cell | increased number, with Scer\GAL4VP16.nos.UTR

      (Leatherman and DiNardo, 2008)

      male germline stem cell | increased number, with Scer\GAL4A3.eya

      (Leatherman and DiNardo, 2008)

      melanotic mass, with Scer\GAL4Cg.PA

      (Asha et al., 2003)

      melanotic mass, with Scer\GAL4He.PZ

      (Zettervall et al., 2004)

      melanotic mass, with Scer\GAL4Ugt36A1.PK

      (Kimbrell et al., 2002)

      testis, with Scer\GAL4A3.eya

      (Leatherman and DiNardo, 2008)

      testis, with Scer\GAL4VP16.nos.UTR

      (Leatherman and DiNardo, 2008)
      Detailed Description
      Statement
      Reference

      Expression of hopTum.UAS under the control of Scer\GAL4ey.PU leads to an increase in the size of eye.

      (Harsh et al., 2020)

      Expression of hopTum.Scer\UAS under the control of Scer\GAL4C587 results in germaria with an increase in the number of spectrosome-containing cells, as compared to controls.

      Expression of hopTum.Scer\UAS under the control of Scer\GAL4GMR25A11 results in germaria with an increase in the number of spectrosome-containing cells, as compared to controls.

      (Liu et al., 2015)

      Expression of hopTum.Scer\UAS under the control of Scer\GAL4Myo31DF-NP0001 (using tub-gal80[ts] to limit the time of expression) increases cell proliferation in the adult gut.

      (Wang et al., 2015)

      Expression of hopTum.Scer\UAS under the control of Scer\GAL4byn-Gal4 results in stage 15 embryonic hindguts having a lower of angle curvature, as compared to controls.

      (Wells et al., 2013)

      Expression of hopTum.Scer\UAS under the control of Scer\GAL4elav.Switch.PO in the presence of RU486 results in defects in locomotor activity rhythms (only 16.7% of flies show rhythmic behaviour under constant darkness conditions).

      (Luo and Sehgal, 2012)

      Expression of hopTum.Scer\UAS under the control of Scer\GAL4C855a results in strong overproliferation of the outer optic anlagen.

      (Richter et al., 2011)

      Expression of hopTum.Scer\UAS under the control of Scer\GAL4slbo.2.6 does not result in early border cell migration.

      (Jang et al., 2009)

      Expression of hopTum.Scer\UAS in germ cells under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 results in accumulation of germ cells away from the hub. However, the gonial region appears normal with no evidence of increased germline stem cell self renewal. Expression of hopTum.Scer\UAS in somatic cells under the control of Scer\GAL4A3.eya causes the testes to fill with early stage cells, including cyst progenitor cells and germline stem cells.

      (Leatherman and DiNardo, 2008)

      Expression of hopTum.Scer\UAS under the control of Scer\GAL4ey.PU results in overgrowth of the adult eye.

      (Karsten et al., 2006)

      Ectopic expression of hopTum.Scer\UAS, under the control of Scer\GAL4c306, induces large numbers of ectopic migrating border cells. These cells migrate in a variety of sizes of clusters, which lack polar cells, and can migrate as individual cells.

      (Silver et al., 2005)

      Expression of hopTum.Scer\UAS under the control of Scer\GAL4stop.Hml results in an elevation in the number of circulating larval hemocytes compared to wild type.

      (Sinenko and Mathey-Prevot, 2004)

      Expression of hopTum.Scer\UAS under the control of Scer\GAL4bi-omb-Gal4 results in an enlarged eye primarily along the dorsal-ventral axis.

      (Tsai and Sun, 2004)

      When expression is driven by Scer\GAL4He.PZ, hemocyte proliferation is unaffected, lamellocyte numbers are increased and crystal cells are unaffected. Melanotic masses form.

      (Zettervall et al., 2004)

      Larvae expressing hopTum.Scer\UAS under the control of Scer\GAL4Cg.PA have melanotic tumours and their hemolymph contains an increased number of plasmatocytes and lamellocytes compared to wild type. The number and morphology of hemocytes is similar to wild type in larvae expressing hopTum.Scer\UAS under the control of Scer\GAL4en-e16E.

      (Asha et al., 2003)

      Expression of hopTum.Scer\UAS under the control of Scer\GAL4ey.PH, Scer\GAL4elav.PLu or Scer\GAL4GMR.PF results in flies with enlarged eyes.

      (Bach et al., 2003)

      Expression of hopTum.Scer\UAS under the control of Scer\GAL4byn-Gal4 results in embryos with shorter and wider hindguts than normal.

      (Johansen et al., 2003)

      Expression of hopTum.Scer\UAS under the control of Scer\GAL4Dot.PK results in melanotic tumours in larvae. Many of the larvae die, although some survive to become tumorous adults (the degree of lethality depends on the P{Dot-GAL4.K} line used).

      (Kimbrell et al., 2002)

      When hopScer\UAS.cHa is driven by Scer\GAL4c306 or Scer\GAL4slbo.2.6, there is an increase in the number of border cells compared to wild-type.

      (Silver and Montell, 2001)

      Heat shock during second or third instar larval development is sufficient to produce melanotic masses within larvae and pupae and cause death.

      (Harrison et al., 1995)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Scer\GAL4ey.PU, hopTum.UAS has eye phenotype, suppressible | partially by ZIKV\NS4AUAS.Tag:FLAG/Scer\GAL4ey.PU

      (Harsh et al., 2020)
      Suppressor of
      Statement
      Reference

      hopTum.UAS, Scer\GAL4slbo.2.6 is a suppressor of border follicle cell phenotype of Rac1N17.UAS, Scer\GAL4slbo.2.6

      (Silver, 2005)

      Scer\GAL4ey.PH/hopTum.UAS is a suppressor of eye phenotype of upd11

      (Tsai and Sun, 2004)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of hopTum.Scer\UAS and taiΔB.Scer\UAS under the control of Scer\GAL4slbo.2.6 results in border cell migration 12 hours earlier than normal in 38% of egg chambers.

      (Jang et al., 2009)

      Expression of hopTum.Scer\UAS in the spiracles, driven by Scer\GAL4klu-G410, results in partial rescue of the Df(1)os1A2 spiracle defect.

      (Hombria et al., 2005)

      Expression of hopTum.Scer\UAS suppresses the border cell migration defect caused by expression of Rac1N17.Scer\UAS under the control of Scer\GAL4slbo.2.6.

      (Silver, 2005)

      Expression of hopTum.Scer\UAS under the control of Scer\GAL4ey.PH rescues the small eye phenotype of Df(1)os1 mutant flies.

      (Tsai and Sun, 2004)

      Expression of hopTum.Scer\UAS under the control of Scer\GAL4drm.7.1 fails to rescue the mutant hindgut phenotype of Df(1)os1A2 embryos.

      (Johansen et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      HopTuml
      (Karsten et al., 2006)
      hopTum-1
      (Krzemień et al., 2007)
      hopTum.Scer\UAS
      hopTum.UAS
      hopTuml
      (Bausek and Zeidler, 2014)
      Name Synonyms
      Secondary FlyBase IDs
        References (43)