FlyBase Allele Report: Dmel\Src42A[k10108]

(Hildebrand et al., 2021, O'Donnell and Bashaw, 2013, Tikhmyanova et al., 2010, Laberge et al., 2005, Takahashi et al., 2005, Lu and Li, 1999, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Kaltenbach et al., 2007)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult midgut | conditional

(Cordero et al., 2014)

embryo | dorsal closure stage

(Lu and Li, 1999)

intestinal stem cell of posterior adult midgut epithelium | conditional

(Cordero et al., 2014)

larval head

(Tikhmyanova et al., 2010)

larval tracheal system

(Tikhmyanova et al., 2010, Lu and Li, 1999)

Detailed Description

Statement

Reference

In Src42A^k10108/+ wandering third instar larvae CadN-immunopositive adherens junctions between photoreceptor precursor cells R3 and R4 in row 5 have similar lengths as wild-type controls.

(Pütz, 2019)

Src42A^k10108/+ adults show a significantly impaired intestinal stem cell proliferation (regeneration) response in midguts after feeding flies with pathogenic bacteria.

(Cordero et al., 2014)

Src42A^k10108 homozygous embryos hatch, but die as first instar larvae from defects in tail morphology, head involution, and tracheal necrosis.

(Tikhmyanova et al., 2010)

Homozygotes die as first instar larvae. Src42A^k10108/Df(2R)nap9 animals show defects in head involution, tail morphology and tracheal necrosis.

(Lu and Li, 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Src42A^k10108/Src42A^E1 has abnormal neuroanatomy phenotype, enhanceable by Src64B^ko

(O'Donnell and Bashaw, 2013)

Enhancer of

Statement

Reference

Src42A^k10108/Src42A[+] is an enhancer of visible | adult stage phenotype of Scer\GAL4^lz-gal4, Shrm^A.UAS

(Hildebrand et al., 2021)

Src42A^k10108/Src42A[+] is an enhancer of visible | adult stage phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

Src42A^k10108 is an enhancer of visible phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Kaltenbach et al., 2007)

Suppressor of

Statement

Reference

Src42A^k10108/Src42A[+] is a suppressor | partially of abnormal cell adhesion | wandering third instar larval stage phenotype of mbt^P1

(Pütz, 2019)

Src42A^k10108/Src42A[+] is a suppressor | partially of abnormal size | wandering third instar larval stage phenotype of mbt^P1

(Pütz, 2019)

Src42A^k10108/Src42A[+] is a suppressor | partially of abnormal neuroanatomy | wandering third instar larval stage phenotype of mbt^P1

(Pütz, 2019)

Src42A^k10108/Src42A[+] is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^eg-Mz360, fra^{ΔC.UAS.Tag:HA}

(O'Donnell and Bashaw, 2013)

Src42A^k10108, Src42A[+], Src64B[+], Src64B^ko is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^eg-Mz360, fra^{ΔC.UAS.Tag:HA}

(O'Donnell and Bashaw, 2013)

Src42A^k10108, Src42A[+], Src64B^ko is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^eg-Mz360, fra^{ΔC.UAS.Tag:HA}

(O'Donnell and Bashaw, 2013)

Src42A^k10108 is a suppressor of visible phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

Src42A^k10108 is a suppressor of visible phenotype of Ret^MEN2A.GMR

(Read et al., 2005)

NOT Suppressor of

Statement

Reference

Src42A^k10108 is a non-suppressor of lethal | pupal stage phenotype of Csk^j1D8/Csk^S030003

(Read et al., 2004)

Src42A^k10108 is a non-suppressor of lethal phenotype of Raf¹

(Lu and Li, 1999)

Other

Statement

Reference

Src42A^k10108/Src42A^E1, Src64B[+]/Src64B^ko has abnormal neuroanatomy phenotype

(O'Donnell and Bashaw, 2013)

Src42A^k10108/Src42A[+], p130CAS¹ has partially lethal - majority live | embryonic stage phenotype

(Tikhmyanova et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Src42A^k10108/Src42A^E1 has fascicle phenotype, enhanceable by Src64B^ko

(O'Donnell and Bashaw, 2013)

Src42A^k10108/Src42A^E1 has symmetrical commissure phenotype, enhanceable by Src64B^ko

(O'Donnell and Bashaw, 2013)

Enhancer of

Statement

Reference

Src42A^k10108/Src42A[+] is an enhancer of wing phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

Src42A^k10108/Src42A[+] is an enhancer of wing phenotype of Scer\GAL4^lz-gal4, Shrm^A.UAS

(Hildebrand et al., 2021)

Src42A^k10108 is an enhancer of eye phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Kaltenbach et al., 2007)

NOT Enhancer of

Statement

Reference

Src42A^k10108 is a non-enhancer of eye phenotype of Ras85D^V12.sev

(Laberge et al., 2005)

Src42A^k10108 is a non-enhancer of ommatidium phenotype of Ras85D^V12.sev

(Laberge et al., 2005)

Suppressor of

Statement

Reference

Src42A^k10108/Src42A[+] is a suppressor | partially of photoreceptor R3 precursor cell | wandering third instar larval stage phenotype of mbt^P1

(Pütz, 2019)

Src42A^k10108/Src42A[+] is a suppressor | partially of photoreceptor R4 precursor cell | wandering third instar larval stage phenotype of mbt^P1

(Pütz, 2019)

Src42A^k10108/Src42A[+] is a suppressor | partially of adherens junction | wandering third instar larval stage phenotype of mbt^P1

(Pütz, 2019)

Src42A^k10108/Src42A[+] is a suppressor of larval EW neuron phenotype of Scer\GAL4^eg-Mz360, fra^{ΔC.UAS.Tag:HA}

(O'Donnell and Bashaw, 2013)

Src42A^k10108/Src42A[+] is a suppressor of symmetrical commissure phenotype of Scer\GAL4^eg-Mz360, fra^{ΔC.UAS.Tag:HA}

(O'Donnell and Bashaw, 2013)

Src42A^k10108, Src42A[+], Src64B[+], Src64B^ko is a suppressor of larval EW neuron phenotype of Scer\GAL4^eg-Mz360, fra^{ΔC.UAS.Tag:HA}

(O'Donnell and Bashaw, 2013)

Src42A^k10108, Src42A[+], Src64B[+], Src64B^ko is a suppressor of symmetrical commissure phenotype of Scer\GAL4^eg-Mz360, fra^{ΔC.UAS.Tag:HA}

(O'Donnell and Bashaw, 2013)

Src42A^k10108, Src42A[+], Src64B^ko is a suppressor of larval EW neuron phenotype of Scer\GAL4^eg-Mz360, fra^{ΔC.UAS.Tag:HA}

(O'Donnell and Bashaw, 2013)

Src42A^k10108, Src42A[+], Src64B^ko is a suppressor of symmetrical commissure phenotype of Scer\GAL4^eg-Mz360, fra^{ΔC.UAS.Tag:HA}

(O'Donnell and Bashaw, 2013)

Src42A^k10108 is a suppressor of eye phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

Src42A^k10108 is a suppressor of eye phenotype of Ret^MEN2A.GMR

(Read et al., 2005)

Other

Statement

Reference

Src42A^k10108/Src42A^E1, Src64B[+]/Src64B^ko has symmetrical commissure phenotype

(O'Donnell and Bashaw, 2013)

Src42A^k10108/Src42A^E1, Src64B[+]/Src64B^ko has fascicle phenotype

(O'Donnell and Bashaw, 2013)

Src42A^k10108, p130CAS¹ has embryonic head phenotype

(Tikhmyanova et al., 2010)

Src42A^k10108, p130CAS¹ has embryo | late embryonic stage phenotype

(Tikhmyanova et al., 2010)

Src42A^k10108, p130CAS¹ has larva | first instar larval stage phenotype

(Tikhmyanova et al., 2010)

Src42A^k10108/Src42A[+], p130CAS¹ has wing phenotype

(Tikhmyanova et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

GluRIIA^SP16/GluRIIA^SP16 Csk^c04256/Csk^c04256 Src42A^k10108/+ third instar larvae show significant decreases in mEPSP (decreased quantal size) but do not show significant compensatory homeostatic increases in quantal content (similar to GluRIIA^SP16/GluRIIA^SP16 Csk^c04256/Csk^c04256 double mutants) at the NMJ. GluRIIA^SP16/GluRIIA^SP16 Src42A^k10108/+ third instar larvae look similar to GluRIIA^SP16/GluRIIA^SP16 larvae (significant decreases in mEPSP and compensatory homeostatic increases in quantal content) at the NMJ.

(Spring et al., 2016)

A Src42A^k10108 heterozygous background partially suppresses the eg-positive neuron midline crossing defects found upon expression of fra^{ΔC.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^eg-Mz360.

A Src42A^k10108/+ ; Src64B^ko/+ trans-heterozygous background partially suppresses the eg-positive neuron midline crossing defects found upon expression of fra^{ΔC.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^eg-Mz360.

Src42A^k10108/+;Src64B^ko heterozygous background suppresses the eg-positive neuron midline crossing defects found upon expression of fra^{ΔC.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^eg-Mz360.

Src42A^E1/Src42A^k10108 ; Src64B^ko/+ mutants display occasional wandering/defasciculation in commissural axon pathfinding but remain ipsilateral in the embryonic central nervous system.

Src42A^E1/Src42A^k10108 ; Src64B^ko/Src64B^ko double mutants exhibit severe defects in Fas2-positive axons, with Fas2-positive ipsilateral axons often crossing the midline inappropriately. eg-positive commissural neurons do not exhibit defects in these mutants.

(O'Donnell and Bashaw, 2013)

Synthetic lethality is found in p130CAS¹ homozygotes with a Src42A^k10108 heterozygous or homozygous background, with fewer than 46% of the double mutant embryos hatch. 90% of the cuticles of the double mutants have holes in or absence of the head cuticle and 10% of the double mutants exhibit additional germ band retraction defects.

Src42A^k10108/+; p130CAS¹/Df(3L)Exel6083 mutants are semi-viable, emerging at approximately 50% of the rate of phenotypically normal adult siblings from the same cross.

The low percentage of Src42A^k10108/+; p130CAS¹/p130CAS¹ adult escapers manifest wing blister defects.

Approximately 90% of Src42A^k10108; p130CAS¹ double mutants have holes in or completely absent head cuticles and 10% of these embryos exhibit germ band retraction cuticle defects.

(Tikhmyanova et al., 2010)

Does not suppress the lethality of phl¹.

(Lu and Li, 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Src42A^unspecified

(Tateno et al., 2000)

Src42A^Su(Raf)1-1

(Lu and Li, 1999)