FlyBase Allele Report: Dmel\svp[1]

svp¹/svp^11C115 mutants exhibit Eya-positive Ap neuron loss in 30% thoracic hemisegments. In the hemisegments in which Ap neurons are generated an increase in Ap neuron number is observed, from 4 to approximately 6 cells. Extra Ap1/Nplp1 neurons are seen and there is loss of the Ap4/FMRFa cell fate.

(Benito-Sipos et al., 2011)

Homozygous clones in the retina have ommatidia which lack some out photoreceptor cells.

(Gambis et al., 2011)

svp¹ homozygous mutant animals show a significant increase in the numer of SE2 neurons specified.

(Losada-Pérez et al., 2010)

svp¹ mutants produce the normal number of fully developed and attaching alary muscles. Although occasioanl patterning defects are observed, these are not more prevalent in svp¹ mutants than in controls.

(LaBeau et al., 2009)

Individual svp¹ mutant R1 and R6 clone cells (generated using MARCM) terminate in the R8 target layer. When R7 photoreceptors are ablated using sev^V1, svp¹ mutant R1 and R6 axons terminate in the R7 and R8 layers of the medulla with approximately equal frequency.

50% of svp¹ mutant R1 and 53% of R6 clone cells have adopted characteristics usually seen in R8 cells: expression of R8 rhodopsins, small rhabdomeres (rather than the large ones usually seen), and nuclei that are located proximal to R1, R6 and R7 nuclei. A further 42% of svp¹ mutant R1s and 41% of R6s have adopted R7 characteristics, having small rhabdomeres that contain R7 rhodopsins. The nuclei are not displaced proximally in these cells. Few R1/R6 cells are seen that express both R7 and R8 rhodopsins, and there is a strong correlation between cells that express R7 or R8 rhodopsins and those that target the equivalent layer of the medulla, indicating that svp¹ mutant R1 and R6 cells take on either an R7 or an R8 cell fate, not a mixed fate. None of the mutant R1s and R6s form a regular spatial pattern. svp¹ mutant R1/R6 cells are directed towards R7 or R8 fates shortly after their recruitment during larval development.

76% of svp¹ mutant R4 clone cells in otherwise wild type ommatidia have small rhabdomeres, rather than the large ones usually seen, and also lack the R1-R6 specific rhodopsin ninaE. Approximately half express R7 rhodopsins and half express R8 rhodopsins.

77% of svp¹ mutant R3 clone cells in otherwise wild type ommatidia retain the usual large outer rhabdomere and continue to express the R1-R6 specific rhodopsin ninaE. The remainder adopting R7 or R8 fates with equal likelihood. A similar R7/R8 conversion is seen in svp¹ mutant mystery cells. The mutant R3s with large outer rhabdomeres are in ommatidia with reversed chirality, indicating that they have adopted an R4 cell fate.

Eyes wholly mutant for svp¹ (generated using W^GMR.PG) contain increased numbers of R7 and R8 cells (2.2 and 2.5 per ommatidium respectively).

(Miller et al., 2008)

The total number of photoreceptor cells in the Bolwig's organ of mutant embryos is normal. However, all the photoreceptors are of the Rh5-positive subtype and no Rh6-positive photoreceptors are seen.

(Sprecher et al., 2007)

svp¹ mutants exhibit a reduction in the number of NB6-4T-derived neurons in almost all hemineuromeres.

(Mettler et al., 2006)

Photoreceptor cells R3, R4, R1 and R6 are transformed into R7 cells.

(Iwanami et al., 2005)

svp¹/svp² embryos have extra U1 neurons at the expense of later born U neurons from the same (NB7-1) lineage. U4 and U5 neurons are particularly depleted. Similarly, there are increased numbers of early born cells from the NB 7-3 lineage (EW1 and GW) compared to later born cells from this lineage.

(Kanai et al., 2005)

In homozygous clones in the eye, most ommatidia have three to five cells with small rhabdomeres.

(Domingos et al., 2004)

svp¹ mutant embryos have an estimated 90 cells in each Malpighian tubule cell (as opposed to about 125 in wild-type).

(Sudarsan et al., 2002)

R1, R3, R4 and R6 are transformed to R7 cells. Targets chosen by R2 and R5 are invariably misorientated with respect to the equator.

(Clandinin and Zipursky, 2000)

Mosaic ommatidia at the border of homozygous clones in the eye show polarity defects, including misrotations, wrong chirality or symmetrical, non-chiral ommatidia. When one cell of the R3/R4 pair is mutant in mosaic ommatidia, the ommatidium adopts the wrong chiral form and the svp^- cell assumes the R4 position. Mosaic ommatidia with mutant R1 or R6 cells behave normally. Misrotated R3/R4 precursor pairs are seen in homozygous clones in the third larval instar eye disc. These cells are misrotated by approximately 45^o or 90^o from their normally oriented neighbours.

(Fanto et al., 1998)

Homozygous embryos have a reduced number of cells in both the anterior and posterior Malpighian tubules compared to wild-type embryos. Tip cell determination occurs normally in these embryos. BrdU incorporation appears relatively normal during the initial cell divisions of the Malpighian tubules, but subsequently it is strongly reduced indicating a failure of DNA replication.

(Kerber et al., 1998)

In svp mutant clones the R1 and R6 cells are missing.

(Hiromi et al., 1993)

embryonic lethal

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

svp¹/svp[+] is an enhancer of visible phenotype of Scer\GAL4^sev.PU, Zzzz\CTG^i480.UAS.cGa

(Garcia-Lopez et al., 2008)

Suppressor of

Statement

Reference

svp¹ is a suppressor of lethal | recessive phenotype of kay²

(Gritzan et al., 2002)

Other

Statement

Reference

sens^E2, svp¹ has lethal - all die during embryonic stage phenotype

(Mishra et al., 2013)

svp¹, tll¹ has visible | somatic clone phenotype

(Gritzan et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

svp²/svp¹ has EW2 neuron phenotype, enhanceable by Kr^mCD, Kr¹/Kr¹

(Kanai et al., 2005)

svp²/svp¹ has EW3 neuron phenotype, enhanceable by Kr^mCD, Kr¹/Kr¹

(Kanai et al., 2005)

svp²/svp¹ has larval DA3 motor neuron phenotype, enhanceable by Kr^mCD, Kr¹/Kr¹

(Kanai et al., 2005)

svp²/svp¹ has larval LL1 motor neuron phenotype, enhanceable by Kr^mCD, Kr¹/Kr¹

(Kanai et al., 2005)

Suppressed by

Statement

Reference

Kr¹, Kr^mCD, svp²/svp¹ has EW3 neuron phenotype, suppressible | partially by hb¹⁵/hb¹²

(Kanai et al., 2005)

Kr¹, Kr^mCD, svp²/svp¹ has larval DA3 motor neuron phenotype, suppressible | partially by hb¹⁵/hb¹²

(Kanai et al., 2005)

Kr¹, Kr^mCD, svp²/svp¹ has larval LL1 motor neuron phenotype, suppressible | partially by hb¹⁵/hb¹²

(Kanai et al., 2005)

Enhancer of

Statement

Reference

svp¹/svp[+] is an enhancer of eye phenotype of Scer\GAL4^sev.PU, Zzzz\CTG^i480.UAS.cGa

(Garcia-Lopez et al., 2008)

svp¹ is an enhancer of wing vein | ectopic phenotype of Scer\GAL4^32B, rl^Sem.C.UAS

(Gritzan et al., 2002)

Suppressor of

Statement

Reference

svp¹ is a suppressor of embryo | dorsal closure stage phenotype of kay¹/kay²

(Gritzan et al., 2002)

Other

Statement

Reference

sens^E2, svp¹ has Rh5 photoreceptor of Bolwig organ phenotype

(Mishra et al., 2013)

sens^E2, svp¹ has Rh6 photoreceptor of Bolwig organ | increased number phenotype

(Mishra et al., 2013)

N^act.sev, svp¹ has photoreceptor cell R7 | ectopic | somatic clone phenotype

(Miller et al., 2008)

svp¹, tll¹ has wing vein | ectopic | somatic clone | cell autonomous phenotype

(Gritzan et al., 2002)

svp¹, tll¹ has wing | somatic clone | cell autonomous phenotype

(Gritzan et al., 2002)

ro^x63, svp¹ has ommatidium phenotype

(Heberlein et al., 1991)

Additional Comments

Genetic Interactions

Statement

Reference

sens^E2 svp¹ double mutant embryos have the same number of photoreceptors as wild type, but none of them express Rh5 and an increased number express Rh6.

(Mishra et al., 2013)

N^act.sev expression in svp¹ mutant R1/R6 cells (generated using W^GMR.PG) results in all cells transforming to a R7 cell fate, as opposed to either an R7 or R8 fate as is seen in cells that are only mutant for svp¹.

(Miller et al., 2008)

Kr^mCD; Kr¹/Kr¹ enhances the reduction in later born neurons of the NB7-1 an NB2-3 lineages see in svp¹/svp² embryos so that U4 and U5 are almost completely eliminated, as are EW2 and EW3. This enhanced phenotype is partially suppressed if the embryos are also hb¹²/hb¹⁵.

(Kanai et al., 2005)

svp¹ Df(2L)32FP-5 double mutant ommatidia have the same phenotype as Df(2L)32FP-5 single mutant ommatidia in clones in the eye; small rhabdomeres are not seen.

(Domingos et al., 2004)

kay²/kay² svp¹ flies are viable, and sometimes have mild thorax closure defects. tll¹ svp¹ double mutant clones in the wing show formation of ectopic vein tissue. This phenotype is approximately 100% penetrant and is cell autonomous.

(Gritzan et al., 2002)

Double mutant clones with ro^x63, in otherwise wild type ommatidia, contain only central type receptor cells. The overall number of photoreceptor cells in each ommatidium is the same in svp^-,ro^- and svp⁺,ro^- clones.

(Heberlein et al., 1991)

Xenogenetic Interactions

Statement

Reference

svp¹ dominantly enhances the rough eye phenotype caused by expression of Zzzz\CTG^{i480.Scer\UAS.cGa} under the control of Scer\GAL4^sev.PU.

(Garcia-Lopez et al., 2008)

Complementation and Rescue Data

Fails to complement

svp^sd-22

(Babcock et al., 2003)

svp^sd-27

(Babcock et al., 2003)

svp^don1

(Gould, 2000.3.8)

svp^j2E2

(Hoshizaki et al., 1995)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

6190

108674

Notes on Origin

Discoverer

Comments

No interaction with P{sev-svp1} or P{sev-svp2} exists.

(Begemann et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

cK16^e22

ck16e022

(Parkes et al., 1993)

e22

(Mlodzik et al., 1990)

e²²

(Gausz et al., 1981)

l(3)ck16^e22

seven up^e22

(Gambis et al., 2011)

svp¹

(An et al., 2023, King et al., 2021, Ren et al., 2017, Stratmann et al., 2016, Trujillo et al., 2016, Berndt et al., 2015, Bivik et al., 2015, Dong et al., 2014, Chai et al., 2013, Losada-Pérez et al., 2013, Benito-Sipos et al., 2011, Kuzin et al., 2011, Losada-Pérez et al., 2010, LaBeau et al., 2009, Garcia-Lopez et al., 2008)

svp^E22

(Mishra et al., 2016, Mishra et al., 2013, Sprecher et al., 2007, Wernet et al., 2003)

Name Synonyms

Secondary FlyBase IDs

References (51)

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