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Citation
Gambis, A., Dourlen, P., Steller, H., Mollereau, B. (2011). Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila.  Dev. Biol. 351(1): 128--134.
FlyBase ID
FBrf0213000
Publication Type
Research paper
Abstract

We report a new two-color fluorescent imaging system to visualize the mosaic adult photoreceptor neurons (PRs) in real-time. Using this method, we examined a collection of 434 mutants and identified genes required for PR survival, planar cell polarity (PCP), patterning and differentiation. We could track the progression of PR degeneration in living flies. By introducing the expression of p35, a caspase inhibitor, we found mutations that specifically activate caspase-dependent death. Moreover, we showed that grh is required in R3 for correct PCP establishment. The "Tomato/GFP-FLP/FRT" method allows high-throughput, rapid and precise identification of survival and developmental pathways in living adult PRs at single-cell resolution.

PubMed ID
PubMed Central ID
PMC3051417 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Dev. Biol.
    Title
    Developmental Biology
    Publication Year
    1959-
    ISBN/ISSN
    0012-1606
    Data From Reference
    Aberrations (1)
    Alleles (16)
    Genes (18)
    Natural transposons (1)
    Experimental Tools (3)
    Transgenic Constructs (4)