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General Information
Symbol
Dmel\emcAP6
Species
D. melanogaster
Name
FlyBase ID
FBal0051626
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Deletion of approximately 400bp of coding sequence and the Tirant element present in the emcD chromosome is lost.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

emcΔ1/emcAP6 mutants rescued by the expression of emcUAS.cdCa under the control of Scer\GAL4Act.PU survive to pharate adults, and a small proportion emerge as adults; both adults and pharate adults exhibit almost normal eye development, including the interommatidial bristles, an essentially normal pattern of thoracic macrochaetae, despite the lack of most microchaetae, a spaced pattern of some microchaetae, and a reduced by essentially normal pattern of wing margin bristles, as compared to controls.

Homozygous mutant emcAP6 cells survive in the larval and pupal stages if the mutant clones are induced in a background heterozygous for a minute mutation (RpS17unspecified). It is possible to obtain eye imaginal discs almost entirely comprised of emcAP6 mutant cells, when clones are generated using Scer\FLP1ey.PN induced recombination in a trans-heterozygous emcAP6-RpS17unspecified background.

emcAP6 mutant eye discs have an overall narrower shape. Morphogenetic furrow progression is accelerated in the ventral half compared to that of dorsal half. Patterning of the developing eye field is severely affected. The number of photoreceptor neurons per ommatidium is irregular as is the arrangement of photoreceptor neurons within ommatidial clusters. Occasionally, ectopic differentiating neurons are seen ahead of the morphogenetic furrow. The number of cone cells is significantly reduced. The apical nuclear migration typical of cone cells is delayed or absent in ommatidia. R7 photoreceptor cells are almost completely lost in eye imaginal discs almost entirely comprised of emcAP6 mutant cells. However, in smaller emcAP6 mutant clones, the presumptive R7 photoreceptor cells maintain their neuronal identity, but they fail to differentiate appropriately and maintain the R7 receptor fate. This effect is cell autonomous. emcAP6 mutant cells in the R7 position display R1/6-like properties in the form of ectopic svp expression. In emcAP6 mutant eye discs, ommatidia rotate normally in the dorsal half, whereas almost all ventral ommatidia rotate abnormally in the same direction as in the dorsal half. R4 photoreceptor specification is also affected in emcAP6 mutants.

The density of cells in homozygous follicle cell clones is higher than their wild-type neighbours.

Embryos exhibit defects of the Malpighian tubules and midgut.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Phenotype Manifest In
Suppressed by
Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

da3/emcAP6 double mutant eye disc clones survive in a non-minute background but lack neuronal differentiation. Neither ectopic neuronal differentiation ahead of the furrow nor ectopic morphogenetic furrow initiation from the eye margins is observed.

Homozygous emcAP6 clones only survive in imaginal discs when induced in a background heterozygous for RpS17unspecified. emcAP6 clones accelerate the morphogenetic furrow and often initiate ectopic morphogenetic furrows from the lateral eye margin. Clones are also associated with sporadic neuronal differentiation anterior to the furrow and in the peripodial membrane, although such ectopic neurons do not adopt the R8 photoreceptor cell fate.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Strong emc allele.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
References (11)