4 kb deletion
da10 mutant embryos lack the entire peripheral nervous system and defects in the central nervous system compared to wild type embryos.
Approximately 2% of da10 heterozygous flies lose their post-vertical head bristles.
Sensory organ precursors are absent in homozygous clones in the third instar wing disc.
Photoreceptor R8 cells are not seen in clones in the eye disc expressing daΔTADs.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Act.PU in a da10 background.
Photoreceptor R8 cells can form in clones in the eye disc expressing either daΔAD1.Scer\UAS.T:Hsap\MYC or daΔLH.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Act.PU in a da10 background.
da10 mutant eye disc clones grow in a non-minute background but fail to initiate neurogenesis.
The gl-positive corpora cardiaca precursor cells are missing in stage 12 mutant embryos.
Homozygous intestinal stem cell clones fail to grow and are composed of single or pairs of differentiated enterocyte cells.
da10 mutant clones generated in the wing disc produce bald patches on the adult thorax where sensory bristles are missing.
The ectopic neuron phenotype produced by expression of amosScer\UAS.cHa under the control of Scer\GAL4sca-537.4 is suppressed by one copy of da10.
Homozygous clonal tissue within the eye causes a narrow anterior-posterior scar. Larger clones in the anterior of the eye are observed, in the centre of the clone several mutant ommatidia surround an area lacking photoreceptor cells. Homozygous clone encompassing the morphogenetic furrow interrupts the progression of the furrow. Clones located anterior to and within the furrow exhibit normal cell division. Clones located posterior to the furrow exhibit suppression of cell proliferation.
SNSPs are reduced or absent. SNS pouches are irregular. Typically a single, large SNS pouch, which may be branched, invaginates from the stomodeal epithelium. No SNSPs appear posteriorly and the ventricular ganglion, which is derived from the posterior pouch, is absent.
Loss of neuron mutant.
Homozygous embryos lack all sensory organs, have hypoplasic defects and various defects in the somatic musculature and midgut.
The phenotype of the double mutant Df(1)sc-B57; da10 is more severe than either of the mutations alone: additive effects contribute to the phenotype.
da10 has abnormal neuroanatomy | embryonic stage phenotype, suppressible by Hsap\TCF4UAS.A/Scer\GAL4da.G32
da10 has abnormal neuroanatomy | embryonic stage phenotype, suppressible by Scer\GAL4da.G32/Hsap\TCF4UAS.B
da10 has lethal | recessive | embryonic stage phenotype, non-suppressible by da::Drer\tcf3abHLH
da10 has lethal | recessive | embryonic stage phenotype, non-suppressible by da::Drer\tcf3aHLH
da[+]/da10 is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4sca-537.4, amosUAS.cHa
da10 has head bristle phenotype, enhanceable by sc[+]/sc10-1
da10 has embryonic/larval peripheral nervous system | embryonic stage phenotype, suppressible by Hsap\TCF4UAS.A/Scer\GAL4da.G32
da10 has presumptive embryonic/larval central nervous system | embryonic stage phenotype, suppressible by Hsap\TCF4UAS.A/Scer\GAL4da.G32
da10 has embryonic/larval peripheral nervous system | embryonic stage phenotype, suppressible by Scer\GAL4da.G32/Hsap\TCF4UAS.B
da10 has presumptive embryonic/larval central nervous system | embryonic stage phenotype, suppressible by Scer\GAL4da.G32/Hsap\TCF4UAS.B
da10 has sense organ | embryonic stage phenotype, suppressible by da::Drer\tcf3abHLH
da10 has embryonic/larval midgut | embryonic stage phenotype, suppressible by da::Drer\tcf3abHLH
da10 has embryonic/larval somatic muscle cell phenotype, suppressible by da::Drer\tcf3aHLH
da10 has sense organ | embryonic stage phenotype, suppressible by da::Drer\tcf3aHLH
da10 has embryonic/larval midgut | embryonic stage phenotype, suppressible by da::Drer\tcf3aHLH
da10 has embryonic/larval somatic muscle cell phenotype, suppressible by da::Drer\tcf3abHLH
da[+]/da10 is an enhancer of head bristle phenotype of sc10-1
da[+]/da10 is an enhancer of photoreceptor neuron | heat sensitive phenotype of Df(3R)p13/ato1090
da[+]/da10 is a suppressor of ommatidium phenotype of amosRoi-1
da[+]/da10 is a suppressor of embryonic/larval neuron | ectopic phenotype of Scer\GAL4sca-537.4, amosUAS.cHa
da10 is a non-suppressor of mechanosensory sensory organ | somatic clone phenotype of Scer\GAL4pnr-MD237, nvyVP16.UAS
ato1, da10 has coeloconic sensillum of antennal segment 3 phenotype
ato1, da[+]/da10 has coeloconic sensillum of antennal segment 3 phenotype
The presence of a da10 mutation within clones expressing nvyScer\UAS.T:Hsim\VP16 under the control of Scer\GAL4pnr-MD237 does not suppress the sensory organ phenotype.
Df(2L)M36F-S5/da10 or Df(2L)M36F-S6/da10 double heterozygous flies have a significantly reduced number of sensilla basiconica on the antenna compared to wild-type flies. The flies have only slightly fewer sensilla coeloconica than flies singly heterozygous for da10, Df(2L)M36F-S5 or Df(2L)M36F-S6. ato1/+ ; da10/+ flies have significantly fewer sensilla coeloconica on the antenna than wild-type flies.
Expression of Hsap\TCF4Scer\UAS.A under the control of Scer\GAL4da.G32 suppresses the neuronal phenotypes seen in homozygous da10 mutant embryos. The embryonic lethality is not rescued.
Expression of Hsap\TCF4Scer\UAS.B under the control of Scer\GAL4da.G32 suppresses the neuronal phenotypes seen in homozygous da10 mutant embryos.
Embryonic phenotype can be rescued by constructs carrying da::Brer\E12HLH or da::Brer\E12bHLH but no flies emerge.
da10 is rescued by Scer\GAL4Tub.PU/daUAS.cGa
da10 is rescued by daΔAD1.UAS.Tag:MYC/Scer\GAL4Tub.PU
da10 is rescued by daΔLH.UAS.Tag:MYC/Scer\GAL4Tub.PU
da10 is rescued by Scer\GAL4da.G32/daUAS.cGa
da10 is partially rescued by Scer\GAL4da.G32/daUAS.cGa
da10 is partially rescued by daA600V.UAS/Scer\GAL4da.G32
da10 is partially rescued by Scer\GAL4da.G32/daD501G.UAS
da10 is not rescued by Scer\GAL4da.G32/daR566W.UAS
da10 is not rescued by daR568P.UAS/Scer\GAL4da.G32
da10 is not rescued by Scer\GAL4da.G32/daR566L.UAS
da10 is not rescued by daΔTADs.UAS.Tag:MYC/Scer\GAL4Tub.PU
Expression of daR564H.Scer\UAS under the control of Scer\GAL4da.G32 rescues the neuronal phenotypes seen in homozygous da10 mutant embryos.
Expression of daR566W.Scer\UAS under the control of Scer\GAL4da.G32 is unable to rescue the neuronal phenotypes seen in homozygous da10 mutant embryos.
Expression of daR568P.Scer\UAS under the control of Scer\GAL4da.G32 is unable to rescue the neuronal phenotypes seen in homozygous da10 mutant embryos.
Expression of daA600V.Scer\UAS under the control of Scer\GAL4da.G32 rescues the neuronal phenotypes seen in homozygous da10 mutant embryos. The embryonic lethality is not rescued.
Expression of daD501G.Scer\UAS under the control of Scer\GAL4da.G32 rescues the neuronal phenotypes seen in homozygous da10 mutant embryos. The embryonic lethality is not rescued.
Expression of daR566L.Scer\UAS under the control of Scer\GAL4da.G32 is unable to rescue the neuronal phenotypes seen in homozygous da10 mutant embryos.
Expression of daScer\UAS.cGa under the control of Scer\GAL4da.G32 rescues the neuronal phenotypes seen in homozygous da10 mutant embryos. The embryonic lethality is not rescued.
Expression of daΔTADs.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4tub.PU in da10 clones in the wing disc fails to rescue sensory organ precursor cell formation.
Expression of either daScer\UAS.cGa, daΔAD1.Scer\UAS.T:Hsap\MYC or daΔLH.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4tub.PU in da10 clones in the wing disc restores sensory organ precursor cell formation.
PNS and CNS defects are completely rescued by Scer\GAL4da.G32-mediated expression of daScer\UAS.cGa.
Expression of ase is reduced although the neuroblasts appear morphologically normal.