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General Information
Symbol
Dmel\da10
Species
D. melanogaster
Name
FlyBase ID
FBal0002223
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
daKX136, daUX, daUX136
Key Links
Mutagen
Nature of the Allele
Mutagen
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

4 kb deletion

Caused by aberration
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

da10 mutant embryos lack the entire peripheral nervous system and defects in the central nervous system compared to wild type embryos.

Approximately 2% of da10 heterozygous flies lose their post-vertical head bristles.

Sensory organ precursors are absent in homozygous clones in the third instar wing disc.

Photoreceptor R8 cells are not seen in clones in the eye disc expressing daΔTADs.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Act.PU in a da10 background.

Photoreceptor R8 cells can form in clones in the eye disc expressing either daΔAD1.Scer\UAS.T:Hsap\MYC or daΔLH.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Act.PU in a da10 background.

da10 mutant eye disc clones grow in a non-minute background but fail to initiate neurogenesis.

The gl-positive corpora cardiaca precursor cells are missing in stage 12 mutant embryos.

Homozygous intestinal stem cell clones fail to grow and are composed of single or pairs of differentiated enterocyte cells.

da10 mutant clones generated in the wing disc produce bald patches on the adult thorax where sensory bristles are missing.

The ectopic neuron phenotype produced by expression of amosScer\UAS.cHa under the control of Scer\GAL4sca-537.4 is suppressed by one copy of da10.

Homozygous clonal tissue within the eye causes a narrow anterior-posterior scar. Larger clones in the anterior of the eye are observed, in the centre of the clone several mutant ommatidia surround an area lacking photoreceptor cells. Homozygous clone encompassing the morphogenetic furrow interrupts the progression of the furrow. Clones located anterior to and within the furrow exhibit normal cell division. Clones located posterior to the furrow exhibit suppression of cell proliferation.

SNSPs are reduced or absent. SNS pouches are irregular. Typically a single, large SNS pouch, which may be branched, invaginates from the stomodeal epithelium. No SNSPs appear posteriorly and the ventricular ganglion, which is derived from the posterior pouch, is absent.

Loss of neuron mutant.

Homozygous embryos lack all sensory organs, have hypoplasic defects and various defects in the somatic musculature and midgut.

The phenotype of the double mutant Df(1)sc-B57; da10 is more severe than either of the mutations alone: additive effects contribute to the phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Statement
Reference
Suppressor of
Statement
Reference

da[+]/da10 is a suppressor | partially of visible | dominant phenotype of amosTft

Phenotype Manifest In
Enhanced by
Statement
Reference

da10 has head bristle phenotype, enhanceable by sc[+]/sc10-1

Suppressed by
Enhancer of
Statement
Reference

da[+]/da10 is an enhancer of head bristle phenotype of sc10-1

da[+]/da10 is an enhancer of eye | heat sensitive phenotype of Df(3R)p13/ato1090

da[+]/da10 is an enhancer of photoreceptor cell | heat sensitive phenotype of Df(3R)p13/ato1090

Suppressor of
Statement
Reference

da[+]/da10 is a suppressor | partially of macrochaeta | ectopic phenotype of amosTft

da[+]/da10 is a suppressor of ommatidium phenotype of amosRoi-1

NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Approximately 95% of sc10-1/+; da10/+ trans-heterozygous flies exhibit missing post-vertical head bristles.

A da10 heterozygous background gives a dominant moderate enhancement to the ato1090/Df(3R)p13 eye phenotype at 25[o]C.

The presence of a da10 mutation within clones expressing nvyScer\UAS.T:Hsim\VP16 under the control of Scer\GAL4pnr-MD237 does not suppress the sensory organ phenotype.

da10/+ almost completely suppresses the amosRoi-1/+ retina phenotype.

Df(2L)M36F-S5/da10 or Df(2L)M36F-S6/da10 double heterozygous flies have a significantly reduced number of sensilla basiconica on the antenna compared to wild-type flies. The flies have only slightly fewer sensilla coeloconica than flies singly heterozygous for da10, Df(2L)M36F-S5 or Df(2L)M36F-S6. ato1/+ ; da10/+ flies have significantly fewer sensilla coeloconica on the antenna than wild-type flies.

Xenogenetic Interactions
Statement
Reference

Expression of Hsap\TCF4Scer\UAS.A under the control of Scer\GAL4da.G32 suppresses the neuronal phenotypes seen in homozygous da10 mutant embryos. The embryonic lethality is not rescued.

Expression of Hsap\TCF4Scer\UAS.B under the control of Scer\GAL4da.G32 suppresses the neuronal phenotypes seen in homozygous da10 mutant embryos.

Embryonic phenotype can be rescued by constructs carrying da::Brer\E12HLH or da::Brer\E12bHLH but no flies emerge.

Complementation and Rescue Data
Partially rescued by
Comments

Expression of daR564H.Scer\UAS under the control of Scer\GAL4da.G32 rescues the neuronal phenotypes seen in homozygous da10 mutant embryos.

Expression of daR566W.Scer\UAS under the control of Scer\GAL4da.G32 is unable to rescue the neuronal phenotypes seen in homozygous da10 mutant embryos.

Expression of daR568P.Scer\UAS under the control of Scer\GAL4da.G32 is unable to rescue the neuronal phenotypes seen in homozygous da10 mutant embryos.

Expression of daA600V.Scer\UAS under the control of Scer\GAL4da.G32 rescues the neuronal phenotypes seen in homozygous da10 mutant embryos. The embryonic lethality is not rescued.

Expression of daD501G.Scer\UAS under the control of Scer\GAL4da.G32 rescues the neuronal phenotypes seen in homozygous da10 mutant embryos. The embryonic lethality is not rescued.

Expression of daR566L.Scer\UAS under the control of Scer\GAL4da.G32 is unable to rescue the neuronal phenotypes seen in homozygous da10 mutant embryos.

Expression of daScer\UAS.cGa under the control of Scer\GAL4da.G32 rescues the neuronal phenotypes seen in homozygous da10 mutant embryos. The embryonic lethality is not rescued.

Expression of daΔTADs.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4tub.PU in da10 clones in the wing disc fails to rescue sensory organ precursor cell formation.

Expression of either daScer\UAS.cGa, daΔAD1.Scer\UAS.T:Hsap\MYC or daΔLH.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4tub.PU in da10 clones in the wing disc restores sensory organ precursor cell formation.

PNS and CNS defects are completely rescued by Scer\GAL4da.G32-mediated expression of daScer\UAS.cGa.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Expression of ase is reduced although the neuroblasts appear morphologically normal.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
References (34)