FlyBase Allele Report: Dmel\da[10]

General Information

Symbol

Dmel\da¹⁰

Species

D. melanogaster

Name

FlyBase ID

FBal0002223

Feature type

allele

Associated gene

Dmel\da

Associated Insertion(s)

Carried in Construct

Also Known As

da^KX136, da^UX, da^UX136

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

X ray

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Wulbeck et al., 1994)

Mutagen

X ray

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Caused by aberration

Df(2L)da¹⁰

Cytology

Nature of the lesion

Statement

Reference

4 kb deletion

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

corpus cardiacum primordium

(Park et al., 2011)

embryonic/larval midgut | embryonic stage

(Wulbeck et al., 1994)

embryonic/larval peripheral nervous system | embryonic stage

(Tamberg et al., 2015)

embryonic/larval salivary gland

(Chandrasekaran and Beckendorf, 2003)

embryonic/larval somatic muscle cell

(Wulbeck et al., 1994)

embryonic/larval stomatogastric nervous system | precursor

(Hartenstein et al., 1996)

embryonic/larval ventricular ganglion

(Hartenstein et al., 1996)

eye disc | somatic clone

(Bhattacharya and Baker, 2011)

eye disc morphogenetic furrow | somatic clone

(Brown et al., 1996)

eye disc posterior compartment | somatic clone | third instar larval stage

(Brown et al., 1996)

eye photoreceptor cell | somatic clone

(Brown et al., 1996)

head bristle

(Hsiao et al., 2014)

intestinal stem cell | somatic clone

(Bardin et al., 2010)

mechanosensory sensory organ

(Wildonger and Mann, 2005)

neuron

(Sturtevant and Bier, 1995)

presumptive embryonic/larval central nervous system | embryonic stage

(Tamberg et al., 2015)

presumptive embryonic/larval nervous system

(Giebel et al., 1997)

sense organ | embryonic stage

(Wulbeck et al., 1994)

sensory mother cell | somatic clone | third instar larval stage

(Zarifi et al., 2012)

wing disc | somatic clone | third instar larval stage

(Zarifi et al., 2012)

Detailed Description

Statement

Reference

da¹⁰ mutant embryos lack the entire peripheral nervous system and defects in the central nervous system compared to wild type embryos.

(Tamberg et al., 2015)

Approximately 2% of da¹⁰ heterozygous flies lose their post-vertical head bristles.

(Hsiao et al., 2014)

Sensory organ precursors are absent in homozygous clones in the third instar wing disc.

Photoreceptor R8 cells are not seen in clones in the eye disc expressing da^{ΔTADs.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act.PU in a da¹⁰ background.

Photoreceptor R8 cells can form in clones in the eye disc expressing either da^{ΔAD1.Scer\UAS.T:Hsap\MYC} or da^{ΔLH.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Act.PU in a da¹⁰ background.

(Zarifi et al., 2012)

da¹⁰ mutant eye disc clones grow in a non-minute background but fail to initiate neurogenesis.

(Bhattacharya and Baker, 2011)

The gl-positive corpora cardiaca precursor cells are missing in stage 12 mutant embryos.

(Park et al., 2011)

Homozygous intestinal stem cell clones fail to grow and are composed of single or pairs of differentiated enterocyte cells.

(Bardin et al., 2010)

da¹⁰ mutant clones generated in the wing disc produce bald patches on the adult thorax where sensory bristles are missing.

(Wildonger and Mann, 2005)

The ectopic neuron phenotype produced by expression of amos^Scer\UAS.cHa under the control of Scer\GAL4^sca-537.4 is suppressed by one copy of da¹⁰.

(Huang et al., 2000)

Homozygous clonal tissue within the eye causes a narrow anterior-posterior scar. Larger clones in the anterior of the eye are observed, in the centre of the clone several mutant ommatidia surround an area lacking photoreceptor cells. Homozygous clone encompassing the morphogenetic furrow interrupts the progression of the furrow. Clones located anterior to and within the furrow exhibit normal cell division. Clones located posterior to the furrow exhibit suppression of cell proliferation.

(Brown et al., 1996)

SNSPs are reduced or absent. SNS pouches are irregular. Typically a single, large SNS pouch, which may be branched, invaginates from the stomodeal epithelium. No SNSPs appear posteriorly and the ventricular ganglion, which is derived from the posterior pouch, is absent.

(Hartenstein et al., 1996)

Loss of neuron mutant.

(Sturtevant and Bier, 1995)

Homozygous embryos lack all sensory organs, have hypoplasic defects and various defects in the somatic musculature and midgut.

(Wulbeck et al., 1994)

The phenotype of the double mutant Df(1)sc-B57; da¹⁰ is more severe than either of the mutations alone: additive effects contribute to the phenotype.

(Jimenez and Campos-Ortega, 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

da¹⁰ has abnormal neuroanatomy | embryonic stage phenotype, suppressible by Hsap\TCF4^UAS.A/Scer\GAL4^da.G32

(Tamberg et al., 2015)

da¹⁰ has abnormal neuroanatomy | embryonic stage phenotype, suppressible by Scer\GAL4^da.G32/Hsap\TCF4^UAS.B

(Tamberg et al., 2015)

NOT suppressed by

Statement

Reference

da¹⁰ has lethal | recessive | embryonic stage phenotype, non-suppressible by da::Drer\tcf3a^bHLH

(Wulbeck et al., 1994)

da¹⁰ has lethal | recessive | embryonic stage phenotype, non-suppressible by da::Drer\tcf3a^HLH

(Wulbeck et al., 1994)

Suppressor of

Statement

Reference

da[+]/da¹⁰ is a suppressor | partially of visible | dominant phenotype of amos^Tft

(Lai, 2003)

da[+]/da¹⁰ is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^sca-537.4, amos^UAS.cHa

(Huang et al., 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

da¹⁰ has head bristle phenotype, enhanceable by sc[+]/sc^10-1

(Hsiao et al., 2014)

Suppressed by

Statement

Reference

da¹⁰ has embryonic/larval peripheral nervous system | embryonic stage phenotype, suppressible by Hsap\TCF4^UAS.A/Scer\GAL4^da.G32

(Tamberg et al., 2015)

da¹⁰ has presumptive embryonic/larval central nervous system | embryonic stage phenotype, suppressible by Hsap\TCF4^UAS.A/Scer\GAL4^da.G32

(Tamberg et al., 2015)

da¹⁰ has embryonic/larval peripheral nervous system | embryonic stage phenotype, suppressible by Scer\GAL4^da.G32/Hsap\TCF4^UAS.B

(Tamberg et al., 2015)

da¹⁰ has presumptive embryonic/larval central nervous system | embryonic stage phenotype, suppressible by Scer\GAL4^da.G32/Hsap\TCF4^UAS.B

(Tamberg et al., 2015)

da¹⁰ has sense organ | embryonic stage phenotype, suppressible by da::Drer\tcf3a^bHLH

(Wulbeck et al., 1994)

da¹⁰ has embryonic/larval midgut | embryonic stage phenotype, suppressible by da::Drer\tcf3a^bHLH

(Wulbeck et al., 1994)

da¹⁰ has embryonic/larval somatic muscle cell phenotype, suppressible by da::Drer\tcf3a^HLH

(Wulbeck et al., 1994)

da¹⁰ has sense organ | embryonic stage phenotype, suppressible by da::Drer\tcf3a^HLH

(Wulbeck et al., 1994)

da¹⁰ has embryonic/larval midgut | embryonic stage phenotype, suppressible by da::Drer\tcf3a^HLH

(Wulbeck et al., 1994)

da¹⁰ has embryonic/larval somatic muscle cell phenotype, suppressible by da::Drer\tcf3a^bHLH

(Wulbeck et al., 1994)

Enhancer of

Statement

Reference

da[+]/da¹⁰ is an enhancer of head bristle phenotype of sc^10-1

(Hsiao et al., 2014)

da[+]/da¹⁰ is an enhancer of eye | heat sensitive phenotype of Df(3R)p13/ato¹⁰⁹⁰

(Melicharek et al., 2008)

da[+]/da¹⁰ is an enhancer of photoreceptor neuron | heat sensitive phenotype of Df(3R)p13/ato¹⁰⁹⁰

(Melicharek et al., 2008)

Suppressor of

Statement

Reference

da[+]/da¹⁰ is a suppressor | partially of macrochaeta | ectopic phenotype of amos^Tft

(Lai, 2003)

da[+]/da¹⁰ is a suppressor of ommatidium phenotype of amos^Roi-1

(Chanut et al., 2002)

da[+]/da¹⁰ is a suppressor of embryonic/larval neuron | ectopic phenotype of Scer\GAL4^sca-537.4, amos^UAS.cHa

(Huang et al., 2000)

NOT Suppressor of

Statement

Reference

da¹⁰ is a non-suppressor of mechanosensory sensory organ | somatic clone phenotype of Scer\GAL4^pnr-MD237, nvy^VP16.UAS

(Wildonger and Mann, 2005)

Other

Statement

Reference

Df(2L)M36F-S5, da¹⁰ has basiconic sensillum of antennal segment 3 phenotype

(Goulding et al., 2000)

Df(2L)M36F-S6, da¹⁰ has basiconic sensillum of antennal segment 3 phenotype

(Goulding et al., 2000)

ato¹, da¹⁰ has coeloconic sensillum of antennal segment 3 phenotype

(Goulding et al., 2000)

ato¹, da[+]/da¹⁰ has coeloconic sensillum of antennal segment 3 phenotype

(Goulding et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Approximately 95% of sc^10-1/+; da¹⁰/+ trans-heterozygous flies exhibit missing post-vertical head bristles.

(Hsiao et al., 2014)

A da¹⁰ heterozygous background gives a dominant moderate enhancement to the ato¹⁰⁹⁰/Df(3R)p13 eye phenotype at 25[o]C.

(Melicharek et al., 2008)

The presence of a da¹⁰ mutation within clones expressing nvy^{Scer\UAS.T:Hsim\VP16} under the control of Scer\GAL4^pnr-MD237 does not suppress the sensory organ phenotype.

(Wildonger and Mann, 2005)

da¹⁰/+ almost completely suppresses the amos^Roi-1/+ retina phenotype.

(Chanut et al., 2002)

Df(2L)M36F-S5/da¹⁰ or Df(2L)M36F-S6/da¹⁰ double heterozygous flies have a significantly reduced number of sensilla basiconica on the antenna compared to wild-type flies. The flies have only slightly fewer sensilla coeloconica than flies singly heterozygous for da¹⁰, Df(2L)M36F-S5 or Df(2L)M36F-S6. ato¹/+ ; da¹⁰/+ flies have significantly fewer sensilla coeloconica on the antenna than wild-type flies.

(Goulding et al., 2000)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\TCF4^Scer\UAS.A under the control of Scer\GAL4^da.G32 suppresses the neuronal phenotypes seen in homozygous da¹⁰ mutant embryos. The embryonic lethality is not rescued.

Expression of Hsap\TCF4^Scer\UAS.B under the control of Scer\GAL4^da.G32 suppresses the neuronal phenotypes seen in homozygous da¹⁰ mutant embryos.

(Tamberg et al., 2015)

Embryonic phenotype can be rescued by constructs carrying da::Brer\E12^HLH or da::Brer\E12^bHLH but no flies emerge.

(Wulbeck et al., 1994)

Complementation and Rescue Data

Fails to complement

da^AB12

(Melicharek et al., 2008)

da^IB21

(Melicharek et al., 2008)

Rescued by

da¹⁰ is rescued by Scer\GAL4^Tub.PU/da^UAS.cGa

(Zarifi et al., 2012)

da¹⁰ is rescued by da^{ΔAD1.UAS.Tag:MYC}/Scer\GAL4^Tub.PU

(Zarifi et al., 2012)

da¹⁰ is rescued by da^{ΔLH.UAS.Tag:MYC}/Scer\GAL4^Tub.PU

(Zarifi et al., 2012)

da¹⁰ is rescued by Scer\GAL4^da.G32/da^UAS.cGa

(Giebel et al., 1997)

Partially rescued by

da¹⁰ is partially rescued by Scer\GAL4^da.G32/da^UAS.cGa

(Tamberg et al., 2015)

da¹⁰ is partially rescued by da^A600V.UAS/Scer\GAL4^da.G32

(Tamberg et al., 2015)

da¹⁰ is partially rescued by Scer\GAL4^da.G32/da^D501G.UAS

(Tamberg et al., 2015)

Not rescued by

da¹⁰ is not rescued by Scer\GAL4^da.G32/da^R566W.UAS

(Tamberg et al., 2015)

da¹⁰ is not rescued by da^R568P.UAS/Scer\GAL4^da.G32

(Tamberg et al., 2015)

da¹⁰ is not rescued by Scer\GAL4^da.G32/da^R566L.UAS

(Tamberg et al., 2015)

da¹⁰ is not rescued by da^{ΔTADs.UAS.Tag:MYC}/Scer\GAL4^Tub.PU

(Zarifi et al., 2012)

Comments

Expression of da^{R564H.Scer\UAS} under the control of Scer\GAL4^da.G32 rescues the neuronal phenotypes seen in homozygous da¹⁰ mutant embryos.

Expression of da^{R566W.Scer\UAS} under the control of Scer\GAL4^da.G32 is unable to rescue the neuronal phenotypes seen in homozygous da¹⁰ mutant embryos.

Expression of da^{R568P.Scer\UAS} under the control of Scer\GAL4^da.G32 is unable to rescue the neuronal phenotypes seen in homozygous da¹⁰ mutant embryos.

Expression of da^{A600V.Scer\UAS} under the control of Scer\GAL4^da.G32 rescues the neuronal phenotypes seen in homozygous da¹⁰ mutant embryos. The embryonic lethality is not rescued.

Expression of da^{D501G.Scer\UAS} under the control of Scer\GAL4^da.G32 rescues the neuronal phenotypes seen in homozygous da¹⁰ mutant embryos. The embryonic lethality is not rescued.

Expression of da^{R566L.Scer\UAS} under the control of Scer\GAL4^da.G32 is unable to rescue the neuronal phenotypes seen in homozygous da¹⁰ mutant embryos.

Expression of da^Scer\UAS.cGa under the control of Scer\GAL4^da.G32 rescues the neuronal phenotypes seen in homozygous da¹⁰ mutant embryos. The embryonic lethality is not rescued.

(Tamberg et al., 2015)

Expression of da^{ΔTADs.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^tub.PU in da¹⁰ clones in the wing disc fails to rescue sensory organ precursor cell formation.

Expression of either da^Scer\UAS.cGa, da^{ΔAD1.Scer\UAS.T:Hsap\MYC} or da^{ΔLH.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^tub.PU in da¹⁰ clones in the wing disc restores sensory organ precursor cell formation.

(Zarifi et al., 2012)

PNS and CNS defects are completely rescued by Scer\GAL4^da.G32-mediated expression of da^Scer\UAS.cGa.

(Giebel et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5531

w¹¹¹⁸; Df(2L)da¹⁰, da¹⁰ P{white-un1}30C P{neoFRT}40A/In(2LR)Gla, wg^Gla-1 PPO1^Bc

Kyoto

108281

w¹¹¹⁸; Df(2L)da¹⁰, da¹⁰ P{white-un1}30C P{neoFRT}40A/In(2LR)Gla, wg^Gla-1 PPO1^Bc

Notes on Origin

Discoverer

Comments

Expression of ase is reduced although the neuroblasts appear morphologically normal.

(Brand et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Df(2L)da^KX136

(Giebel et al., 1997)

da¹⁰

(Tamberg et al., 2020, Ishibashi et al., 2019, Sato et al., 2016, Tamberg et al., 2015, Hsiao et al., 2014, Tanaka-Matakatsu et al., 2014, Zarifi et al., 2012, Bhattacharya and Baker, 2011, Park et al., 2011, Bardin et al., 2010, Melicharek et al., 2008)

da^KX136

(Lai, 2003, Giebel et al., 1997, Hassan and Vaessin, 1997, Hoch et al., 1994, Vassin et al., 1994, Wulbeck et al., 1994, Brand et al., 1993, Jimenez and Campos-Ortega, 1990)

da^UX136

(Chanut et al., 2002, Brown et al., 1996)

da^UX

(Distefano et al., 2012, Melicharek et al., 2008, Chanut et al., 2002)

da^X136

(Mackay and Fry, 1996)

da^kx136

(Huang et al., 2000, Chen and Chien, 1999, Sturtevant and Bier, 1995, Edgar et al., 1994, Ip et al., 1994)

Name Synonyms

Secondary FlyBase IDs

References (34)

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