FlyBase Allele Report: Dmel\amos[Tft]

General Information

Symbol

Dmel\amos^Tft

Species

D. melanogaster

Name

Tufted

FlyBase ID

FBal0016764

Feature type

allele

Associated gene

Dmel\amos

Associated Insertion(s)

Carried in Construct

Also Known As

Tft¹, Tft

Key Links

Allele class

gain of function allele

Mutagen

X ray

Nature of the Allele

Allele class

gain of function allele

(Villa-Cuesta et al., 2003)

Mutagen

X ray

Progenitor genotype

Caused by aberration

Dp(2;2)amos^Tft

Cytology

Description

Duplication and translocation of sequences from 36F3-36F7 to 37A. The proximal limit of the duplication has not been determined, but extends a minimum of 75kb upstream of amos. The distal limit of the duplication terminates in the vicinity of amos but its structure is complex.

(Lai, 2003)

Small rearrangement of the amos chromosomal region, most probably a duplication of several kilobases including the amos gene.

(Villa-Cuesta et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible | dominant

(Lai, 2003, Villa-Cuesta et al., 2003, Sur et al., 1995)

visible | recessive

(Held, 1990)

Phenotype Manifest In

abdominal dorsal bipolar neuron dbp | ectopic

(Villa-Cuesta et al., 2003)

adult mesothoracic segment

(Villa-Cuesta et al., 2003)

antennal sense organ | ectopic

(Villa-Cuesta et al., 2003)

chaeta | ectopic

(Villa-Cuesta et al., 2003)

dorsocentral bristle | increased number

(Sur et al., 1995)

embryonic abdominal dorsal sensory organ precursor cluster

(Villa-Cuesta et al., 2003)

macrochaeta | ectopic

(Lai, 2003, Villa-Cuesta et al., 2003)

mesonotum

(Lai, 2003)

mesothoracic metatarsus

(Held, 1990)

mesothoracic tarsal bristle

(Held, 1990)

metathoracic laterotergite

(Lai, 2003, Villa-Cuesta et al., 2003)

microchaeta | ectopic

(Lai, 2003)

postalar bristle | increased number

(Sur et al., 1995)

scutellar bristle | increased number

(Sur et al., 1995)

scutellum

(Lai, 2003, Villa-Cuesta et al., 2003)

sensory mother cell | ectopic

(Lai, 2003, Villa-Cuesta et al., 2003)

tormogen cell

(Villa-Cuesta et al., 2003)

wing disc

(Lai, 2003, Villa-Cuesta et al., 2003)

Detailed Description

Statement

Reference

Heterozygotes have many ectopic macrochaetae and microchaetae. Ectopic bristles are particularly seen in the postalar, dorsocentral and scutellar regions of the notum. Tufts of closely spaced or even adjacent bristles are seen. Ectopic bristles are also seen in the anterior-central portion of the scutellum and the metathoracic notum. Ectopic sensory organ precursor cells form in the presumptive posterior notum and scutellum of heterozygous wing discs.

(Lai, 2003)

Heterozygotes and homozygotes have extra bristles mainly in the postalar, dorsocentral and scutellar regions of the dorsal mesothorax. Ectopic bristles and other sensory organs are also formed in the metanotum, dorsal to the halteres, in heterozygotes. The extra bristles seen in heterozygotes and homozygotes can develop very close together, forming tufts, and in some cases several tormogens can be fused together. Heterozygotes and homozygotes have a reduced scutellum. The average number of ectopic macrochaetae per heminotum is 23 +/- 3 in heterozygous flies. Ectopic sensory bristles are occasionally seen in the antennae of heterozygous flies, while the arrangement of the olfactory sensilla is essentially normal. A large number of extra sensory organ precursor cells (SOPs) form in the presumptive posterior notum in the wing disc in heterozygotes. These extra SOPs form very close together and their appearance is sequential and occurs concomitantly with the normal SOPs. amos^Tft embryos show an increase in the number of neurons in the dorsal cluster of the peripheral nervous system. Occasionally the dorsal bipolar neuron is duplicated.

(Villa-Cuesta et al., 2003)

Heterozygotes have an increased number of bristles in the postalar (13-18), dorsocentral (5-10) and scutellar (7-10) regions of the thorax (normal numbers are 4-5, 3-4 and 4-5 respectively).

(Sur et al., 1995)

The total number of bristles on the basitarsi of the second legs is slightly increased.

(Held, 1990)

Extra bristles often very close together; underlying neurons make functional contacts with the CNS (Ghysen and Richelle, 1979). Innervated bristles also found in the metanotum, which has no bristles in wild-type flies (Garcia-Bellido and Deak). Dominant mutation characterized by an increased number of bristles in the postalar, dorsocentral and scutellar regions; tufts of bristles formed on mesothorax in both homo- and heterozygotes; bristles shorter in homozygotes. Extra bristles located dorsal to halteres at junction of thorax and abdomen. Posterior part of mesonotum appears wider than normal. Homo- and heterozygous females have a greatly reduced scutellum; scutoscutellar suture almost absent; heterozygous males have a nearly normal scutellum (Arnheim, 1967). Small to moderate amounts of fluid tend to remain between the epithelial layers of the wing. Penetrance of extra-bristle character 100%. amos not suppressed by Df(1)sc19 but suppressed by Df(1)260-1 (Garcia-Bellido, communicated to Campuzano, Carramolino, Cabrera, Ruiz-Gomez, Villares, Boronat and Modolell, 1985). Cell autonomous in mosaics (Arnheim, 1967). Viability and fertility low.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

amos^Tft has visible | dominant phenotype, enhanceable by Brd[+]/Brd¹

(Lai, 2003)

amos^Tft has visible | dominant phenotype, enhanceable by Delta^M1/Dl[+]

(Villa-Cuesta et al., 2003)

amos^Tft has visible | dominant phenotype, enhanceable by emc¹/emc¹¹

(Villa-Cuesta et al., 2003)

Suppressed by

Statement

Reference

amos^Tft has visible | dominant phenotype, suppressible by da[+]/da¹⁰

(Lai, 2003)

amos^Tft has visible | dominant phenotype, suppressible by emc^D/emc^D

(Villa-Cuesta et al., 2003)

amos^Tft has visible | dominant phenotype, suppressible by Df(1)ase-1

(Villa-Cuesta et al., 2003)

amos^Tft has visible | dominant phenotype, suppressible by Scer\GAL4^C-765/E(spl)m8-HLH^UAS.cSa

(Villa-Cuesta et al., 2003)

amos^Tft has visible | dominant phenotype, suppressible by H²/H[+]

(Villa-Cuesta et al., 2003)

amos^Tft has visible | dominant phenotype, suppressible by N^Ax-M1/N[+]

(Villa-Cuesta et al., 2003)

NOT suppressed by

Statement

Reference

amos^Tft has visible | dominant phenotype, non-suppressible by Df(1)sc10-1

(Lai, 2003, Villa-Cuesta et al., 2003)

Enhancer of

Statement

Reference

amos^Tft/amos[+] is an enhancer of visible | adult stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

Phenotype Manifest In

Enhanced by

Statement

Reference

amos^Tft has macrochaeta | ectopic phenotype, enhanceable by Brd[+]/Brd¹

(Lai, 2003)

amos^Tft has macrochaeta | ectopic phenotype, enhanceable by Delta^M1/Dl[+]

(Villa-Cuesta et al., 2003)

amos^Tft has macrochaeta | ectopic phenotype, enhanceable by emc¹/emc¹¹

(Villa-Cuesta et al., 2003)

NOT Enhanced by

Statement

Reference

amos^Tft has phenotype, non-enhanceable by ac^T52.2

(Lai, 2003)

Suppressed by

Statement

Reference

amos^Tft has macrochaeta | ectopic phenotype, suppressible by da[+]/da¹⁰

(Lai, 2003)

amos^Tft has macrochaeta | ectopic phenotype, suppressible by Df(1)ase-1

(Villa-Cuesta et al., 2003)

amos^Tft has macrochaeta | ectopic phenotype, suppressible by Scer\GAL4^C-765/E(spl)m8-HLH^UAS.cSa

(Villa-Cuesta et al., 2003)

amos^Tft has macrochaeta | ectopic phenotype, suppressible by H²/H[+]

(Villa-Cuesta et al., 2003)

amos^Tft has macrochaeta | ectopic phenotype, suppressible by N^Ax-M1/N[+]

(Villa-Cuesta et al., 2003)

amos^Tft has macrochaeta | ectopic phenotype, suppressible by emc^D/emc^D

(Villa-Cuesta et al., 2003)

NOT suppressed by

Statement

Reference

amos^Tft has macrochaeta | ectopic phenotype, non-suppressible by Df(1)sc10-1

(Lai, 2003, Villa-Cuesta et al., 2003)

Enhancer of

Statement

Reference

amos^Tft/amos[+] is an enhancer of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

Suppressor of

Statement

Reference

amos^Tft is a suppressor of microchaeta | ectopic & scutellum phenotype of hry¹

(Villa-Cuesta et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The amos^Tft/+ phenotype ectopic bristle phenotype is enhanced by Brd¹/+. amos^Tft is epistatic to Df(1)sc10-1/Y - Df(1)sc10-1/Y ; amos^Tft/+ flies have ectopic sensory organs in the regions seen in amos^Tft/+ animals.

(Lai, 2003)

The average number of ectopic macrochaetae per heminotum in amos^Tft/+ flies is increased to 35 +/- 6 if they are also carrying emc¹/emc¹¹. The average number of ectopic macrochaetae per heminotum in amos^Tft/+ flies is increased to 33 +/- 5 if they are also carrying Dl^M1/+. The average number of ectopic macrochaetae per heminotum in amos^Tft/+ flies is reduced to 16 +/- 4 if they are also carrying N^Ax-M1/+. The average number of ectopic macrochaetae per heminotum in amos^Tft/+ flies is reduced to 19 +/- 2 if they are also carrying H²/+. The average number of ectopic macrochaetae per heminotum in amos^Tft/+ flies is reduced to 6 +/- 2 if they are also expressing E(spl)^Scer\UAS.cSa under the control of Scer\GAL4^C-765. The average number of ectopic macrochaetae per heminotum in amos^Tft/+ flies is reduced to 4 +/- 2 if they are also carrying emc^D/emc^D. The ectopic microchaetae seen on the scutellum in h¹ flies are suppressed by amos^Tft. The tufts of ectopic bristles on the notum that are seen in amos^Tft/+ flies are suppressed by Df(1)ase-1, but are unaffected by Df(1)sc10-1.