FlyBase Allele Report: Dmel\N[Ax-M1]

General Information

Symbol

Dmel\N^Ax-M1

Species

D. melanogaster

Name

FlyBase ID

FBal0029940

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

Also Known As

Ax^M1, N^AxM1, Abruptex^M1

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

antimorphic allele - genetic evidence

gain of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(de Celis and Garcia-Bellido, 1994, Mari-Beffa et al., 1991)

gain of function allele

(Dominguez et al., 2004, de Celis et al., 1996, Mari-Beffa et al., 1991)

hypomorphic allele - genetic evidence

(Brennan et al., 1997)

Mutagen

ethyl methanesulfonate

(de Celis et al., 1991)

Progenitor genotype

Cytology

Description

Carries a mutation in the 25th EGF like repeat of the extracellular domain of N.

(Brennan et al., 1999)

Amino acid replacement: C999Y. C999Y falls in EGF repeat 25.

(Brennan et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:3,165,624..3,165,624 [+]

Nucleotide change:

G3165624A

Amino acid change:

C999Y | N-PA; C999Y | N-PB

Reported amino acid change:

C999Y

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Brennan et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

anterior supraalar bristle

(Escudero et al., 2005)

dorsocentral bristle

(Escudero et al., 2005)

macrochaeta

(Escudero et al., 2003, de Celis and Garcia-Bellido, 1994, de Celis et al., 1991)

macrochaeta | ectopic

(de Celis and Garcia-Bellido, 1994)

microchaeta

(de Celis and Garcia-Bellido, 1994, de Celis et al., 1991)

posterior notopleural bristle

(Escudero et al., 2005)

posterior postalar bristle

(Escudero et al., 2005)

posterior supraalar bristle

(Escudero et al., 2005)

presutural bristle

(Escudero et al., 2005)

proneural cluster & dorsal mesothoracic disc

(Brennan et al., 1999)

scutellar bristle

(Escudero et al., 2005)

wing

(de Celis and Garcia-Bellido, 1994)

wing & macrochaeta

(de Celis and Garcia-Bellido, 1994)

wing vein

(Herranz et al., 2006, Lai and Rubin, 2001, de Celis and Garcia-Bellido, 1994, de Celis and Garcia-Bellido, 1994)

wing vein L5

(Lai and Rubin, 2001)

Detailed Description

Statement

Reference

Heterozygotes show loss of wing vein tissue.

(Herranz et al., 2006)

Heterozygotes lack several notal macrochaetae.

(Escudero et al., 2005)

Heterozygotes show loss of macrochaetae on the notum.

(Escudero et al., 2003)

Mutants show a small amount of wing vein truncation mostly at the tip of wing vein L5.

(Lai and Rubin, 2001)

The proneural clusters of the wing disc are generally smaller than wild-type.

(Brennan et al., 1999)

Heterozygotes appear wild-type.

(Brennan et al., 1997)

Rare escapers show length of microchaetae, macrochaetae and longitudinal wing veins is reduced with respect to that of wild type. Heterozygotes with N^Ax-16 N^Ax-71d, N^Ax-E2, N^Ax-9 or N^Ax-1 are lethal with escapers having less severe phenotypes than that of the N^Ax-M1 escapers. Th lethal phase is earlier than for N^Ax-M1, imaginal discs are larger than wild type and appear deformed. Rare pharate adults show failures in wing and leg evagination and morphological abnormalities that are never seen for N^Ax-M1 hemizygotes. The Abruptex phenotype of heteroallelic combinations with viable Abruptex alleles is reduced at 29^oC as compared to 25^oC.

(de Celis and Garcia-Bellido, 1994)

Heterozygotes have larger than normal wings and incomplete vein differentiation, specially in veins IV and V. Mitotic clones can appear anywhere in the wing. Elimination of the vein occurs only when a clone occupying vein territories is present in the dorsal and ventral surfaces. Clones along the wing margin do not cause nicks but are associated with loss of sensory elements in the anterior wing margin and with the differentiation of ectopic wing vein bristles in distal regions of the wing close to the margin.

(de Celis and Garcia-Bellido, 1994)

Phenotypes have topographic pattern specificities. Males have a homogeneous decrease in chaetae number.

(Mari-Beffa et al., 1991)

Escapers lack almost all macrochaetae and microchaetae. Homozygous clones produced during the last stages of the formation of the adult sensory organs do not enter the sensory organ mother cell pathway.

(de Celis et al., 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

N^Ax-M1 has visible phenotype, enhanceable by neur^UAS.cLa/Scer\GAL4^VMQ

(Lai and Rubin, 2001)

N^Ax-M1 has visible phenotype, enhanceable by Scer\GAL4^VMQ/neur^αRING.UAS

(Lai and Rubin, 2001)

Suppressed by

Statement

Reference

N^Ax-M1 has visible phenotype, suppressible by Hsap\PTOV1^UAS.Tag:HA/Scer\GAL4^nub.PU

(Alaña et al., 2014)

NOT suppressed by

Statement

Reference

N^Ax-M1 has visible | dominant phenotype, non-suppressible by ed^slH8/ed^1X5

(Escudero et al., 2003)

Enhancer of

Statement

Reference

N^Ax-M1 is an enhancer of decreased cell number | larval stage | somatic clone phenotype of l(2)gd1^d7

(Klein, 2003)

Suppressor of

Statement

Reference

N^Ax-M1/N[+] is a suppressor of visible | dominant phenotype of Bx¹

(Bejarano et al., 2008)

N^Ax-M1/N[+] is a suppressor of visible phenotype of Scer\GAL4^en-e16E, pzg^RNAi.UAS

(Kugler and Nagel, 2007)

N^Ax-M1/N[+] is a suppressor of visible | dominant phenotype of amos^Tft

(Villa-Cuesta et al., 2003)

N^Ax-M1 is a suppressor of visible | dominant phenotype of fng^D4

(de Celis and Bray, 2000)

Other

Statement

Reference

N^Ax-M1, wg^S107 has visible | dominant phenotype

(Brennan et al., 1997)

Df(3R)Delta-FX3, N^Ax-M1 has visible | dominant phenotype

(Brennan et al., 1997)

Phenotype Manifest In

Enhanced by

Statement

Reference

N^Ax-M1 has dorsocentral bristle phenotype, enhanceable by Scer\GAL4^ap-md544/chn^RNAi.UAS

(Escudero et al., 2005)

N^Ax-M1 has posterior postalar bristle phenotype, enhanceable by Scer\GAL4^ap-md544/chn^RNAi.UAS

(Escudero et al., 2005)

N^Ax-M1 has scutellar bristle phenotype, enhanceable by Scer\GAL4^ap-md544/chn^RNAi.UAS

(Escudero et al., 2005)

N^Ax-M1 has posterior notopleural bristle phenotype, enhanceable by Scer\GAL4^ap-md544/chn^RNAi.UAS

(Escudero et al., 2005)

N^Ax-M1 has wing disc phenotype, enhanceable by l(2)gd1^d7

(Klein, 2003)

N^Ax-M1 has wing vein phenotype, enhanceable by Scer\GAL4^VMQ/neur^αRING.UAS

(Lai and Rubin, 2001)

N^Ax-M1 has wing vein L5 phenotype, enhanceable by neur^UAS.cLa/Scer\GAL4^VMQ

(Lai and Rubin, 2001)

N^Ax-M1 has wing vein phenotype, enhanceable by neur^UAS.cLa/Scer\GAL4^VMQ

(Lai and Rubin, 2001)

N^Ax-M1 has wing vein L5 phenotype, enhanceable by Scer\GAL4^VMQ/neur^αRING.UAS

(Lai and Rubin, 2001)

N^Ax-M1 has phenotype, enhanceable by Egfr^t1/Egfr^f11

(Diaz-Benjumea and Garcia-Bellido, 1990)

NOT Enhanced by

Statement

Reference

N^Ax-M1 has phenotype, non-enhanceable by Su(H)²

(Brennan et al., 1999)

Suppressed by

Statement

Reference

N^Ax-M1 has wing phenotype, suppressible by Hsap\PTOV1^UAS.Tag:HA/Scer\GAL4^nub.PU

(Alaña et al., 2014)

N^Ax-M1 has wing vein L5 phenotype, suppressible by Hsap\PTOV1^UAS.Tag:HA/Scer\GAL4^nub.PU

(Alaña et al., 2014)

NOT suppressed by

Statement

Reference

N^Ax-M1 has macrochaeta phenotype, non-suppressible by ed^slH8/ed^1X5

(Escudero et al., 2003)

N^Ax-M1 has phenotype, non-suppressible by Su(H)²

(Brennan et al., 1999)

Enhancer of

Statement

Reference

N^Ax-M1/N[+] is an enhancer of phenotype of Egfr^t1/Egfr^f11

(Diaz-Benjumea and Garcia-Bellido, 1990)

Suppressor of

Statement

Reference

N^Ax-M1 is a suppressor of wing vein L3 phenotype of Hsap\PRDX1^UAS.cWa, Hsap\PRDX2^UAS.cWa, Psn^UAS.cYa, Scer\GAL4^Bx-MS1096

(Wangler et al., 2011)

N^Ax-M1 is a suppressor of wing vein L5 phenotype of Hsap\PRDX1^UAS.cWa, Hsap\PRDX2^UAS.cWa, Psn^UAS.cYa, Scer\GAL4^Bx-MS1096

(Wangler et al., 2011)

N^Ax-M1 is a suppressor of wing phenotype of Hsap\PRDX1^UAS.cWa, Hsap\PRDX2^UAS.cWa, Psn^UAS.cYa, Scer\GAL4^Bx-MS1096

(Wangler et al., 2011)

N^Ax-M1 is a suppressor of scutellar bristle | ectopic phenotype of Hsap\PRDX1^UAS.cWa, Hsap\PRDX2^UAS.cWa, Psn^UAS.cYa, Scer\GAL4^Bx-MS1096

(Wangler et al., 2011)

N^Ax-M1/N[+] is a suppressor of wing margin phenotype of Bx¹

(Bejarano et al., 2008)

N^Ax-M1/N[+] is a suppressor of wing phenotype of Scer\GAL4^en-e16E, pzg^RNAi.UAS

(Kugler and Nagel, 2007)

N^Ax-M1/N[+] is a suppressor of eye phenotype of Scer\GAL4^ey.PH, fng^UAS.cKa

(Dominguez et al., 2004)

N^Ax-M1/N[+] is a suppressor of macrochaeta | ectopic phenotype of amos^Tft

(Villa-Cuesta et al., 2003)

N^Ax-M1 is a suppressor of wing phenotype of fng^D4

(de Celis and Bray, 2000)

Other

Statement

Reference

N^Ax-M1, wg^S107 has adult thoracic sensillum phenotype

(Brennan et al., 1997)

Df(3R)Delta-FX3, N^Ax-M1 has adult thoracic sensillum phenotype

(Brennan et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

The reduction in wing area seen in flies expressing pzg^{dsRNA.Scer\UAS} under the control of Scer\GAL4^en-e16E is suppressed by N^Ax-M1/+.

(Kugler and Nagel, 2007)

One copy of N^Ax-M1 enhances the small eye phenotype of flies in which fng^Scer\UAS.cKa is expressed under the control of Scer\GAL4^ey.PH.

(Dominguez et al., 2004)

The loss of macrochaetae on the notum seen in N^Ax-M1/+ flies is not rescued by ed^1X5/ed^slH8.

(Escudero et al., 2003)

N^Ax-M1, l(2)gd1^d7 mutant wing discs show an extreme phenotype where the disc loses all visible organisation and forms a rounded ball of cells.

(Klein, 2003)

The average number of ectopic macrochaetae per heminotum in amos^Tft/+ flies is reduced to 16 +/- 4 if they are also carrying N^Ax-M1/+.

(Villa-Cuesta et al., 2003)

Dominantly suppresses the fng^D4 wing scalloping phenotype.

(de Celis and Bray, 2000)

The addition of Su(H)² has no effect on the N^Ax-M1 proneural cluster phenotypes.

(Brennan et al., 1999)

N^Ax-M1 ; wg^S107 double heterozygotes have a reduced number of thoracic bristles compared to wild-type. N^Ax-M1 ; Df(3R)Dl-FX3 double heterozygotes have an increased number of thoracic bristles compared to wild-type.

(Brennan et al., 1997)

N^Ax-M1/+ Egfr^E3/Egfr^E3 flies show a simple additive phenotype. Egfr^t1/Egfr^f11 N^Ax-M1/+ double mutants have a superadditive phenotype.

(Diaz-Benjumea and Garcia-Bellido, 1990)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\PTOV1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^nub.PU suppresses the distal vein L5 loss and increase in wing size seen in N^55e11 mutant flies. There is a significant increase in the number of notches per wing compared with N^55e11 alone.

(Alaña et al., 2014)

Co-expression of Psn^Scer\UAS.cYa, Hsap\PRDX1^Scer\UAS.cWa, and Hsap\PRDX2^Scer\UAS.cWa with Scer\GAL4^Bx-MS1096 in flies carrying N^Ax-M1 only results in residual thickening of the L3 and L5 wing veins, which is significantly less severe than that observed in response to expression of Psn^Scer\UAS.cYa, Hsap\PRDX1^Scer\UAS.cWa, and Hsap\PRDX2^Scer\UAS.cWa in a wild-type background.

The ectopic scutellar macrochaetae of flies co-expressing Psn^Scer\UAS.cYa, Hsap\PRDX1^Scer\UAS.cWa, and Hsap\PRDX2^Scer\UAS.cWa under the control of Scer\GAL4^Bx-MS1096 is nearly completely suppressed by N^Ax-M1.

(Wangler et al., 2011)

Complementation and Rescue Data

Complements

N^su42c

(Brennan et al., 1997)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Abolishes the fng-N interaction through EGF22-36.

(Ju et al., 2000)

Cell autonomous in mosaic analysis carried out in the last stages of the formation of the adult sensory organs.

(de Celis et al., 1991)

See de Celis et al. 1992, Roux's Arch Dev Biol 200:64--76 .

(de Celis et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Abruptex^M1

(Herranz et al., 2006, Pérez et al., 2005)

N^Abx

(Wangler et al., 2011)

N^Ax-M1

(Alaña et al., 2014)

N^AxM1

(Kugler and Nagel, 2007, Dominguez et al., 2004, Apidianakis et al., 1999)

Name Synonyms

Abruptex

(Wangler et al., 2011)

Secondary FlyBase IDs

References (26)

Report Sections

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