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General Information
Symbol
Dmel\fngUAS.cKa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Kim
FlyBase ID
FBal0045645
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
uas-Fng, UAS-Fringe, UAS-fng27
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a 1.38kb EcoRI-BamHI fragment of fng cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of fngScer\UAS.cKa under the control of Scer\GAL4elav-C155 leads to ectopic neuroblasts in the larval optic lobe medulla cortex.

Expression of fngScer\UAS.cKa under the control of Scer\GAL4bab1-Gal4 (limited to the adult stages using Scer\GAL80ts.αTub84B) produces a decrease in the number of germline stem cell and cap cells in one week old female flies compared to controls.

Expression of fngScer\UAS.cKa under the control of Scer\GAL4twi.PG has little effect on eve-positive pericardial cell or DA1 muscle cell number.

Expression of fngScer\UAS.cKa under the control of Scer\GAL4ey.PH results in a small eye phenotype.

Expression of fngScer\UAS.cKa under the control of Scer\GAL4dpp.blk1 results in notched wings.

Expression of fngScer\UAS.cKa, under the regulation of Scer\GAL4ptc-559.1 results in the formation of ectopic margin bristles in the adult wing.

In wing discs expressing fngScer\UAS.cKa under the control of Scer\GAL4ptc-559.1, ventral cells along the anterior-posterior boundary show a rounded cellular morphology and an accumulation of F-actin at the adherens junction characteristic of cells at the dorsoventral boundary.

fngScer\UAS.cKa mutant third instar nota clones (under the regulation of Scer\GAL4mat.αTub67C.T:Hsim\VP16) produce no defect in sensory organ precursor numbers.

Expression of fngScer\UAS.cKa under the control of Scer\GAL4ey.PH results in a reproducible small eye phenotype.

Expression of fngScer\UAS.cKa under the control of Scer\GAL4bi-omb-Gal4 does not significantly affect eye size.

Expression of fngScer\UAS.cKa under the control of Scer\GAL4byn-Gal4 does not have an effect on the formation of the hindgut boundary cells.

When expression is driven in clones by Scer\GAL4unspecified extra polar cells develop at the termini of the egg chamber, though not in the main body follicle cells.

When expression is driven by Scer\GAL4c311 eye size is dramatically reduced and ommatidia are square, though their array is regular.

Expression of fngScer\UAS.cKa in the tibia and tarsal segments under the control of Scer\GAL4Dll-md23 affects only the joints of the leg, resulting in their reduction or complete elimination. Expression of fngScer\UAS.cKa under the control of Scer\GAL4klu-G410 results in no phenotype in the leg.

Inhibition of normal joint formation and lengthwise fusion of the tibia and femur is seen in clones in the leg expressing fngScer\UAS.cKa under the control of Scer\GAL4Act5C.PI. The clones can also result in outgrowth of leg tissue. The outgrowth is composed of a mixture of wild-type cells and cells expressing fngScer\UAS.cKa under the control of Scer\GAL4Act5C.PI. Ectopic joint structures are also seen.

Flip-out somatic clones in the wing disc, in which there is clonal ectopic expression of fngScer\UAS.cKa, fail to respect the dorsal-ventral border.

When expression is driven by Scer\GAL469B at 18oC eyes are not affected. When expression is driven by Scer\GAL469B at 25oC eyes of all progeny are reduced or missing. Photoreceptors fail to develop.

When expression is driven by Scer\GAL4ey.PH, the eye is very much reduced.

Flies expressing fngScer\UAS.cKa under the control of Scer\GAL4l(3)31-1-31-1 do not show any bristle defects.

Flies expressing fngScer\UAS.cKa under the control of Scer\GAL4ptc-559.1 have a nick in the wings and a small ectopic wing margin. Flies expressing fngScer\UAS.cKa under the control of Scer\GAL4klu-G410 lack the anterior dorsocentral bristles of the thorax. Flies expressing fngScer\UAS.cKa under the control of Scer\GAL4Bx-MS1096 have nicked wings with normal veins and a reduction of bristles in the notum.

Overexpression of fngScer\UAS.cKa in a wild-type background under the control of Scer\GAL4ap-md544 produces no phenotype.

Flies expressing fngScer\UAS.cKa under the control of Scer\GAL4ey.PH show dramatic loss of eye tissue. Tissue loss is visible in the developing eye imaginal disc, before the morphogenetic furrow moves across the eye. Eye tissue loss is not seen in flies expressing fngScer\UAS.cKa under the control of Scer\GAL4GMR.PF.

Scer\GAL4hs.PB-mediated expression causes a reduction in viability.

Eliminates wing margin tissue at the tip of the wing and induces ectopic wing margin tissue in ventral cells when expressed using Scer\GAL4ptc-559.1.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Scer\GAL4ey.PH, fngUAS.cKa has eye phenotype, enhanceable by Hipk3/Hipk4

Scer\GAL4ey.PH, fngUAS.cKa has eye phenotype, enhanceable by Dl[+]/DlX

NOT Enhanced by
Statement
Reference

Scer\GAL4ey.PH, fngUAS.cKa has eye phenotype, non-enhanceable by Ser[+]/SerRX106

Scer\GAL4ey.PH, fngUAS.cKa has eye phenotype, non-enhanceable by Ser[+]/SerRX82

Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
Statement
Reference

fngUAS.cKa, Scer\GAL4l(3)31-1-31-1 is a suppressor of presutural bristle & trichogen cell phenotype of DlUAS.cJa, Scer\GAL4l(3)31-1-31-1

fngUAS.cKa, Scer\GAL4l(3)31-1-31-1 is a suppressor of anterior notopleural bristle & trichogen cell phenotype of DlUAS.cJa, Scer\GAL4l(3)31-1-31-1

Scer\GAL4l(3)31-1-31-1/fngUAS.cKa is a suppressor of presutural bristle & trichogen cell phenotype of Scer\GAL4l(3)31-1-31-1, SerUAS.cGa

Scer\GAL4l(3)31-1-31-1/fngUAS.cKa is a suppressor of posterior notopleural bristle & trichogen cell phenotype of Scer\GAL4l(3)31-1-31-1, SerUAS.cGa

Scer\GAL4l(3)31-1-31-1/fngUAS.cKa is a suppressor of anterior supraalar bristle & trichogen cell phenotype of Scer\GAL4l(3)31-1-31-1, SerUAS.cGa

fngUAS.cKa, Scer\GAL4l(3)31-1-31-1 is a suppressor of posterior notopleural bristle & trichogen cell phenotype of DlUAS.cJa, Scer\GAL4l(3)31-1-31-1

Scer\GAL4l(3)31-1-31-1/fngUAS.cKa is a suppressor of posterior supraalar bristle & trichogen cell phenotype of Scer\GAL4l(3)31-1-31-1, SerUAS.cGa

Scer\GAL4l(3)31-1-31-1/fngUAS.cKa is a suppressor of anterior postalar bristle & trichogen cell phenotype of Scer\GAL4l(3)31-1-31-1, SerUAS.cGa

Scer\GAL4l(3)31-1-31-1/fngUAS.cKa is a suppressor of anterior dorsocentral bristle & trichogen cell phenotype of Scer\GAL4l(3)31-1-31-1, SerUAS.cGa

Scer\GAL4l(3)31-1-31-1/fngUAS.cKa is a suppressor of posterior dorsocentral bristle & trichogen cell phenotype of Scer\GAL4l(3)31-1-31-1, SerUAS.cGa

Scer\GAL4l(3)31-1-31-1/fngUAS.cKa is a suppressor of anterior scutellar bristle & trichogen cell phenotype of Scer\GAL4l(3)31-1-31-1, SerUAS.cGa

Scer\GAL4l(3)31-1-31-1/fngUAS.cKa is a suppressor of posterior scutellar bristle & trichogen cell phenotype of Scer\GAL4l(3)31-1-31-1, SerUAS.cGa

fngUAS.cKa, Scer\GAL4l(3)31-1-31-1 is a suppressor of anterior supraalar bristle & trichogen cell phenotype of DlUAS.cJa, Scer\GAL4l(3)31-1-31-1

fngUAS.cKa, Scer\GAL4l(3)31-1-31-1 is a suppressor of anterior postalar bristle & trichogen cell phenotype of DlUAS.cJa, Scer\GAL4l(3)31-1-31-1

fngUAS.cKa, Scer\GAL4l(3)31-1-31-1 is a suppressor of posterior postalar bristle & trichogen cell phenotype of DlUAS.cJa, Scer\GAL4l(3)31-1-31-1

fngUAS.cKa, Scer\GAL4l(3)31-1-31-1 is a suppressor of posterior dorsocentral bristle & trichogen cell phenotype of DlUAS.cJa, Scer\GAL4l(3)31-1-31-1

fngUAS.cKa, Scer\GAL4l(3)31-1-31-1 is a suppressor of anterior scutellar bristle & trichogen cell phenotype of DlUAS.cJa, Scer\GAL4l(3)31-1-31-1

fngUAS.cKa, Scer\GAL4l(3)31-1-31-1 is a suppressor of posterior scutellar bristle & trichogen cell phenotype of DlUAS.cJa, Scer\GAL4l(3)31-1-31-1

Scer\GAL4ap-md544/fngUAS.cKa is a suppressor of wing phenotype of Bx1

Other
Statement
Reference

Scer\GAL4ap-md544, apmd544/apts78j, fngUAS.cKa, mewUAS.cWa has alula & macrochaeta | supernumerary phenotype

Scer\GAL4ap-md544, apmd544/apts78j, fngUAS.cKa, mewUAS.cWa has sensillum campaniformium | supernumerary & wing vein L3 | ventral phenotype

Additional Comments
Genetic Interactions
Statement
Reference

Expression of fngScer\UAS.cKa under the control of Scer\GAL4twi.PG in a spdoG104 background increases eve-positive pericardial cell number and decreases DA1 muscle numbers compared to embryos mutant for spdoG104.

Expression of fngScer\UAS.cKa under the control of Scer\GAL4pros.PMG in a spdoG104 mutant background restores vMP2 development in approximately 60% of hemisegments.

Expression of fngScer\UAS.cKa under the control of Scer\GAL4twi.PG in a numb2 and spdoG104 mutant background increases the number of eve-positive pericardial cells, and decreases the number of eve-positive DA1 muscle numbers compared to embryos mutant for spdoG104. However, there is no change observed in this increased number of eve-positive pericardial cells between spdoG104 embryos also expressing fngScer\UAS.cKa under the control of Scer\GAL4twi.PG alone, or in combination with numb2.

fngScer\UAS.cKa over expression by Scer\GAL4twi.PG in a spdoG104 mutant background results in both daughter cells to adopting a svp-positive pericardial cell fate almost all of the time.

Co-expression of either hipkScer\UAS.T:Ivir\HA1 or groEE.cLa.Scer\UAS partially rescues the small eye phenotype caused by expression of fngScer\UAS.cKa under the control of Scer\GAL4ey.PH.

The small eye phenotype caused by expression of fngScer\UAS.cKa under the control of Scer\GAL4ey.PH is enhanced by hipk3/hipk4.

Flies expressing fngScer\UAS.cKa under the control of Scer\GAL4ap-md544 in a aprK568/apmd544 background have ventralised halteres. In addition, the halteres form dark-pigmented bristles that are similar to those formed at the wing margin.

Adult thoraces of vgnull, Scer\GAL41151, fngScer\UAS.cKa flies show a significant rescue of muscle degeneration compared to thoraces of vgnull flies.

DlScer\UAS.cLa, fngScer\UAS.cKa double-mutant third instar nota clones (under the regulation of Scer\GAL4mat.αTub67C.T:Hsim\VP16) display a significantly higher number of sensory organ precursors per cluster than single mutants. DlRevF10 fngScer\UAS.cKa third instar nota clones (under the regulation of Scer\GAL4mat.αTub67C.T:Hsim\VP16) exhibit at least eight sensory organ precursors (SOPs) in approximately 47% of SOP positions scored. Approximately 12.5% of SOP positions display between four and eight ectopic SOPs, while approximately 28% exhibit between one and four SOPs, with only 12.5% exhibiting one SOP, as in wild-type.

One copy of DlX enhances the small eye phenotype of flies in which fngScer\UAS.cKa is expressed under the control of Scer\GAL4ey.PH. NAx-M1 suppresses this phenotype. However, in flies heterozygous for SerRX82 or SerRX106, the small eye phenotype is not enhanced. Scer\GAL4ey.PH-mediated expression of eygScer\UAS.lune fully rescues the eye size defect caused by Scer\GAL4ey.PH-mediated expression of fngScer\UAS.cKa. This effect is also partially rescued by expression of toeScer\UAS.cAa under the control of Scer\GAL4ey.PH. Scer\GAL4ey.PH-mediated expression of toyScer\UAS.cCa has no effect on the small eye phenotype, while Scer\GAL4ey.PH-mediated expression of eyScer\UAS.cHa can partially rescue the small eye phenotype in some flies but most often enhances this phenotype and induces the expression of tiny eyes in different places of the head.

The Bx1 scalloped wing phenotype is suppressed by expression of fngScer\UAS.cKa under the control of Scer\GAL4ap-md544.

Co-expression of tshScer\UAS.cGa and fngScer\UAS.cKa under the control of Scer\GAL4bi-omb-Gal4 results in suppression of eye development on the ventral margin in the eye disc and adult eye. Clones in the ventral part of the eye that are co-expressing tshScer\UAS.cGa and fngScer\UAS.cKa under the control of Scer\GAL4Act5C.PI show suppression of eye development in the eye disc, whereas clones in the dorsal part of the eye disc that are co-expressing tshScer\UAS.cGa and fngScer\UAS.cKa under the control of Scer\GAL4Act5C.PI induce ectopic eye development.

A sharp boundary between dorsal and ventral cells is restored in aprK568/apmd544 wing discs expressing fngScer\UAS.cKa under the control of Scer\GAL4ap-md544. No violations of the compartment boundary are seen. The resulting adult flies have wings of roughly normal size with clearly demarcated wing margin. The rescued wing margin is ventralised; dorsal-specific sensory bristles are completely missing along the anterior margin and cells on both sides of the margin secrete ventral bristle types. The dorsal and ventral wing surfaces are not adhered to each other, resulting in a balloon-like structure. apUGO35/apmd544 flies expressing fngScer\UAS.cKa under the control of Scer\GAL4ap-md544 do not have wing margin defects. Coexpression of mewScer\UAS.cWa and fngScer\UAS.cKa under the control of Scer\GAL4ap-md544 partially rescues the apts78j/apmd544 wing phenotype; the overall size and shape, apposition of the two surfaces, position of the wing margin and pattern of the wing veins are remarkably normal. However, these wings consist entirely of ventral cell types. Firstly, the rescued wing margins lack the dorsal-specific bristles types and instead have ventral bristle types on both surfaces. Secondly, the wings have many more alula bristles than normal (which in wild-type are derived only from the ventral surface). Thirdly, a mechanosensory campaniform sensillum that is normally located only on the ventral surface of vein L3 is duplicated and present on both surfaces of the rescued wing. Finally, the morphology of the wing veins indicate that they entirely of ventral identity (the veins show a ventral corrugation pattern on both surfaces of the wing).

Flip-out somatic clones in the wing disc, in which there is clonal ectopic expression of both Dl::NΔECN.Scer\UAS and fngScer\UAS.cKa, show a slight but consistent extension into the dorsal compartment.

The eye-size effect can be partially rescued by simultaneous expression of Nint.SH.Scer\UAS, though ommatidia are abnormally arranged.

The transformation of the trichogen to a tormogen fate in the notal macrochaetae caused by SerScer\UAS.cGa expressed under the control of Scer\GAL4l(3)31-1-31-1 is suppressed by co-expression of fngScer\UAS.cKa. The transformation of the trichogen to a tormogen fate in the notal macrochaetae caused by DlScer\UAS.cJa expressed under the control of Scer\GAL4l(3)31-1-31-1 is suppressed by co-expression of fngScer\UAS.cKa.

The loss of wing seen in apUGO35 homozygotes is partially rescued by fngScer\UAS.cKa expressed under the control of Scer\GAL4dpp.blk1. Flies expressing SerScer\UAS.cSa under the control of Scer\GAL4ptc-559.1 have smaller wings than normal which have two ectopic wing margins. The ectopic wing margin structures are reduced to a posterior stripe with characteristics of the posterior compartment if the flies are also co-expressing fngScer\UAS.cKa. The phenotype caused by expressing fngScer\UAS.cKa under the control of Scer\GAL4klu-G410 is not altered if the flies are also mutant for SerCS94/SerRX106. Suppresses the extra vein phenotype produced when SerScer\UAS.cSa is expressed under the control of Scer\GAL4Bx-MS1096.

Scer\GAL4dpp.blk1-mediated expression in apUGO35 mutants elicits an ectopic wing. Wing exhibits DV asymmetries and the margin is on the dorsal side.

The wing scalloping phenotype of Bx1 heterozygotes is fully rescued by fngScer\UAS.cKa expressed under the control of Scer\GAL4ap-md544, but the transformation phenotype of the alula is not.

Scer\GAL4hs.PB-mediated expression of SerScer\UAS.cGa causes death prior to the end of embryogenesis, simultaneous expression with fngScer\UAS.cKa reduces the lethality and restores normal neuronal differentiation, flies develop into phenotypically wild type adults.

Coexpression of SerBd.Scer\UAS.T:Hsap\MYC and fngScer\UAS.cKa via Scer\GAL4hs.PB results in animals with apparently normal CNS development, and apparently improved viability when compared to SerBd.Scer\UAS.T:Hsap\MYC, Scer\GAL4hs.PB alone. Can suppress the early pupal lethality of SerBd.Scer\UAS.T:Hsap\MYC expressed via Scer\GAL4ptc-559.1. Flies survive longer, to late pupal stages, after disc eversion and as pharate adults. The flies cannot eclose but exhibit complete though shortened legs and no wing blades.

Co-expression of fngScer\UAS.cKa with SerScer\UAS.cSa in the wing (using Scer\GAL4ptc-559.1) stimulates ectopic wing margin formation. Ectopic wing margin is formed in ventral cells of the wing when DlScer\UAS.cDa and fngScer\UAS.cKa are co-expressed in the wing using Scer\GAL4ptc-559.1.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
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Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
fngScer\UAS.c.Ga
fngScer\UAS.cKa
fngUAS.cKa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Kim
Secondary FlyBase IDs
    References (55)