Expression of fngScer\UAS.cKa under the control of Scer\GAL4twi.PG in a spdoG104 background increases eve-positive pericardial cell number and decreases DA1 muscle numbers compared to embryos mutant for spdoG104.
Expression of fngScer\UAS.cKa under the control of Scer\GAL4twi.PG in a numb2 and spdoG104 mutant background increases the number of eve-positive pericardial cells, and decreases the number of eve-positive DA1 muscle numbers compared to embryos mutant for spdoG104. However, there is no change observed in this increased number of eve-positive pericardial cells between spdoG104 embryos also expressing fngScer\UAS.cKa under the control of Scer\GAL4twi.PG alone, or in combination with numb2.
Flies expressing fngScer\UAS.cKa under the control of Scer\GAL4ap-md544 in a aprK568/apmd544 background have ventralised halteres. In addition, the halteres form dark-pigmented bristles that are similar to those formed at the wing margin.
DlScer\UAS.cLa, fngScer\UAS.cKa double-mutant third instar nota clones (under the regulation of Scer\GAL4mat.αTub67C.T:Hsim\VP16) display a significantly higher number of sensory organ precursors per cluster than single mutants. DlRevF10 fngScer\UAS.cKa third instar nota clones (under the regulation of Scer\GAL4mat.αTub67C.T:Hsim\VP16) exhibit at least eight sensory organ precursors (SOPs) in approximately 47% of SOP positions scored. Approximately 12.5% of SOP positions display between four and eight ectopic SOPs, while approximately 28% exhibit between one and four SOPs, with only 12.5% exhibiting one SOP, as in wild-type.
One copy of DlX enhances the small eye phenotype of flies in which fngScer\UAS.cKa is expressed under the control of Scer\GAL4ey.PH. NAx-M1 suppresses this phenotype. However, in flies heterozygous for SerRX82 or SerRX106, the small eye phenotype is not enhanced. Scer\GAL4ey.PH-mediated expression of eygScer\UAS.lune fully rescues the eye size defect caused by Scer\GAL4ey.PH-mediated expression of fngScer\UAS.cKa. This effect is also partially rescued by expression of toeScer\UAS.cAa under the control of Scer\GAL4ey.PH. Scer\GAL4ey.PH-mediated expression of toyScer\UAS.cCa has no effect on the small eye phenotype, while Scer\GAL4ey.PH-mediated expression of eyScer\UAS.cHa can partially rescue the small eye phenotype in some flies but most often enhances this phenotype and induces the expression of tiny eyes in different places of the head.
Co-expression of tshScer\UAS.cGa and fngScer\UAS.cKa under the control of Scer\GAL4bi-omb-Gal4 results in suppression of eye development on the ventral margin in the eye disc and adult eye. Clones in the ventral part of the eye that are co-expressing tshScer\UAS.cGa and fngScer\UAS.cKa under the control of Scer\GAL4Act5C.PI show suppression of eye development in the eye disc, whereas clones in the dorsal part of the eye disc that are co-expressing tshScer\UAS.cGa and fngScer\UAS.cKa under the control of Scer\GAL4Act5C.PI induce ectopic eye development.
A sharp boundary between dorsal and ventral cells is restored in aprK568/apmd544 wing discs expressing fngScer\UAS.cKa under the control of Scer\GAL4ap-md544. No violations of the compartment boundary are seen. The resulting adult flies have wings of roughly normal size with clearly demarcated wing margin. The rescued wing margin is ventralised; dorsal-specific sensory bristles are completely missing along the anterior margin and cells on both sides of the margin secrete ventral bristle types. The dorsal and ventral wing surfaces are not adhered to each other, resulting in a balloon-like structure. apUGO35/apmd544 flies expressing fngScer\UAS.cKa under the control of Scer\GAL4ap-md544 do not have wing margin defects. Coexpression of mewScer\UAS.cWa and fngScer\UAS.cKa under the control of Scer\GAL4ap-md544 partially rescues the apts78j/apmd544 wing phenotype; the overall size and shape, apposition of the two surfaces, position of the wing margin and pattern of the wing veins are remarkably normal. However, these wings consist entirely of ventral cell types. Firstly, the rescued wing margins lack the dorsal-specific bristles types and instead have ventral bristle types on both surfaces. Secondly, the wings have many more alula bristles than normal (which in wild-type are derived only from the ventral surface). Thirdly, a mechanosensory campaniform sensillum that is normally located only on the ventral surface of vein L3 is duplicated and present on both surfaces of the rescued wing. Finally, the morphology of the wing veins indicate that they entirely of ventral identity (the veins show a ventral corrugation pattern on both surfaces of the wing).
Flip-out somatic clones in the wing disc, in which there is clonal ectopic expression of both Dl::NΔECN.Scer\UAS and fngScer\UAS.cKa, show a slight but consistent extension into the dorsal compartment.
The eye-size effect can be partially rescued by simultaneous expression of Nint.SH.Scer\UAS, though ommatidia are abnormally arranged.
The transformation of the trichogen to a tormogen fate in the notal macrochaetae caused by SerScer\UAS.cGa expressed under the control of Scer\GAL4l(3)31-1-31-1 is suppressed by co-expression of fngScer\UAS.cKa. The transformation of the trichogen to a tormogen fate in the notal macrochaetae caused by DlScer\UAS.cJa expressed under the control of Scer\GAL4l(3)31-1-31-1 is suppressed by co-expression of fngScer\UAS.cKa.
The loss of wing seen in apUGO35 homozygotes is partially rescued by fngScer\UAS.cKa expressed under the control of Scer\GAL4dpp.blk1. Flies expressing SerScer\UAS.cSa under the control of Scer\GAL4ptc-559.1 have smaller wings than normal which have two ectopic wing margins. The ectopic wing margin structures are reduced to a posterior stripe with characteristics of the posterior compartment if the flies are also co-expressing fngScer\UAS.cKa. The phenotype caused by expressing fngScer\UAS.cKa under the control of Scer\GAL4klu-G410 is not altered if the flies are also mutant for SerCS94/SerRX106. Suppresses the extra vein phenotype produced when SerScer\UAS.cSa is expressed under the control of Scer\GAL4Bx-MS1096.
Scer\GAL4hs.PB-mediated expression of SerScer\UAS.cGa causes death prior to the end of embryogenesis, simultaneous expression with fngScer\UAS.cKa reduces the lethality and restores normal neuronal differentiation, flies develop into phenotypically wild type adults.
Coexpression of SerBd.Scer\UAS.T:Hsap\MYC and fngScer\UAS.cKa via Scer\GAL4hs.PB results in animals with apparently normal CNS development, and apparently improved viability when compared to SerBd.Scer\UAS.T:Hsap\MYC, Scer\GAL4hs.PB alone. Can suppress the early pupal lethality of SerBd.Scer\UAS.T:Hsap\MYC expressed via Scer\GAL4ptc-559.1. Flies survive longer, to late pupal stages, after disc eversion and as pharate adults. The flies cannot eclose but exhibit complete though shortened legs and no wing blades.
Co-expression of fngScer\UAS.cKa with SerScer\UAS.cSa in the wing (using Scer\GAL4ptc-559.1) stimulates ectopic wing margin formation. Ectopic wing margin is formed in ventral cells of the wing when DlScer\UAS.cDa and fngScer\UAS.cKa are co-expressed in the wing using Scer\GAL4ptc-559.1.