FlyBase Allele Report: Dmel\ap[ts78j]

General Information

Symbol

Dmel\ap^ts78j

Species

D. melanogaster

Name

FlyBase ID

FBal0030507

Feature type

allele

Associated gene

Dmel\ap

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

spontaneous

Nature of the Allele

Allele class

Mutagen

spontaneous

(Ringo et al., 1991)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible (with ap^md544)

(O'Keefe and Thomas, 2001, O'Keefe et al., 1998)

visible (with ap^md544), with Scer\GAL4^ap-md544, ap^ΔHD.UAS

(O'Keefe et al., 1998)

visible | heat sensitive

(Ringo et al., 1991)

Phenotype Manifest In

wing (with ap^md544)

(Rincon-Limas et al., 2000, O'Keefe et al., 1998)

wing (with ap^md544), with Scer\GAL4^ap-md544, ap^HA-B.UAS

(Rincon-Limas et al., 2000)

wing (with ap^md544), with Scer\GAL4^ap-md544, ap^ΔHD.UAS

(O'Keefe et al., 1998)

wing | heat sensitive

(Ringo et al., 1991)

wing | heat sensitive (with ap^md544)

(O'Keefe and Thomas, 2001)

wing margin (with ap^md544)

(Rincon-Limas et al., 2000, O'Keefe et al., 1998)

Detailed Description

Statement

Reference

ap^ts78j/ap^md544 flies show a nearly complete loss of wings at 25^oC.

(O'Keefe and Thomas, 2001)

ap^md544/ap^ts78j flies grown at 25^oC have wings that lack most of the wing margin but have good proximo-distal growth. The addition of ap^{HA-B.Scer\UAS} severely reduces proximo-distal growth and wing size.

(Rincon-Limas et al., 2000)

ap^md544/ap^ts78j flies have defective wing margins and unfused wing surfaces at 18^oC. Introduction of ap^{ΔHD.Scer\UAS} into this background (expressed under the control of Scer\GAL4^ap-md544) eliminates the wing blade altogether.

(O'Keefe et al., 1998)

Homozygous ap^ts78j flies were reared at the permissive temperature, 18^oC, wings develop in a quasinormal way, and at the restrictive 25^oC, temperature, wings fail to develop.

(Ringo et al., 1991)

Temperature-sensitive mutation. Heterozygous or homozygous females show reduced levels of ornithine decarboxylase activity relative to wild-type at 29^oC. Heterozygous females are vitellogenic and homozygous females are non-vitellogenic at 29^oC.

(Birnbaum and Gilbert, 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ap^md544/ap^ts78j has visible phenotype, suppressible | partially by Scer\GAL4^ap-md544/mew^UAS.cWa/fng^UAS.cKa

(O'Keefe and Thomas, 2001)

Phenotype Manifest In

Suppressed by

Statement

Reference

ap^md544/ap^ts78j has wing phenotype, suppressible | partially by Scer\GAL4^ap-md544/mew^UAS.cWa/fng^UAS.cKa

(O'Keefe and Thomas, 2001)

Other

Statement

Reference

Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^UAS.cKa, mew^UAS.cWa has wing vein phenotype

(O'Keefe and Thomas, 2001)

Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^UAS.cKa, mew^UAS.cWa has dorsal row of wing sensilla phenotype

(O'Keefe and Thomas, 2001)

Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^UAS.cKa, mew^UAS.cWa has wing margin phenotype

(O'Keefe and Thomas, 2001)

Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^UAS.cKa, mew^UAS.cWa has alula & macrochaeta | supernumerary phenotype

(O'Keefe and Thomas, 2001)

Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^UAS.cKa, mew^UAS.cWa has sensillum campaniformium | supernumerary & wing vein L3 | ventral phenotype

(O'Keefe and Thomas, 2001)

Scer\GAL4^ap-md544, ap^md544/ap^ts78j, fng^UAS.cKa, mew^UAS.cWa has ventral row of wing sensilla | increased number phenotype

(O'Keefe and Thomas, 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Coexpression of mew^Scer\UAS.cWa and fng^Scer\UAS.cKa under the control of Scer\GAL4^ap-md544 partially rescues the ap^ts78j/ap^md544 wing phenotype; the overall size and shape, apposition of the two surfaces, position of the wing margin and pattern of the wing veins are remarkably normal. However, these wings consist entirely of ventral cell types. Firstly, the rescued wing margins lack the dorsal-specific bristles types and instead have ventral bristle types on both surfaces. Secondly, the wings have many more alula bristles than normal (which in wild-type are derived only from the ventral surface). Thirdly, a mechanosensory campaniform sensillum that is normally located only on the ventral surface of vein L3 is duplicated and present on both surfaces of the rescued wing. Finally, the morphology of the wing veins indicate that they entirely of ventral identity (the veins show a ventral corrugation pattern on both surfaces of the wing).

(O'Keefe and Thomas, 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

3498