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General Information
Symbol
Dmel\SerUAS.cGa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Gu
FlyBase ID
FBal0042742
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Ser
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a Ser minigene comprised of cDNA sequence up to position 4037, fused to a 1.2kb genomic fragment encoding the remaining 3' region of the gene.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

anterior dorsocentral bristle & trichogen cell, with Scer\GAL4l(3)31-1-31-1

anterior notopleural bristle & trichogen cell, with Scer\GAL4l(3)31-1-31-1

anterior postalar bristle & trichogen cell, with Scer\GAL4l(3)31-1-31-1

anterior scutellar bristle & trichogen cell, with Scer\GAL4l(3)31-1-31-1

anterior supraalar bristle & trichogen cell, with Scer\GAL4l(3)31-1-31-1

posterior dorsocentral bristle & trichogen cell, with Scer\GAL4l(3)31-1-31-1

posterior notopleural bristle & trichogen cell, with Scer\GAL4l(3)31-1-31-1

posterior postalar bristle & trichogen cell, with Scer\GAL4l(3)31-1-31-1

posterior supraalar bristle & trichogen cell, with Scer\GAL4l(3)31-1-31-1

presutural bristle & trichogen cell, with Scer\GAL4l(3)31-1-31-1

scutum & macrochaeta & trichogen cell, with Scer\GAL4l(3)31-1-31-1

Detailed Description
Statement
Reference

Expression of SerScer\UAS.cGa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 results in an increase in the number of hub cells in the gonad in male larvae compared to controls.

SerScer\UAS.cGa third instar nota clones (under the regulation of Scer\GAL4mat.αTub67C.T:Hsim\VP16) do not affect sensory organ numbers.

Ubiquitous expression of SerScer\UAS.cGa under the control of Scer\GAL4arm.PS or Scer\GAL464B produces no change in the shape, size or pattern of denticles. Ubiquitous expression of SerScer\UAS.cGa under the control of Scer\GAL4arm.PS in a SerRX106/Df(3R)Bd background results in severe morphological defects; the mouth hooks develop additional sclerotic material at the middle and base and about a quarter of the embryos show duplication of the mouth hook tips.

Flies expressing SerScer\UAS.cGa under the control of Scer\GAL4l(3)31-1-31-1 show transformation of the trichogen to a tormogen fate in the notal macrochaetae. The transformation does not affect all notal macrochaetae equally. Some transformation of the microchaetae is seen in these flies.

Scer\GAL4ptc-559.1-mediated expression causes cell proliferation in the ventral compartment of the wing imaginal disc. Scer\GAL4Ser.PGF-mediated expression allows nearly normal wing development, adult wings are largely normal with the exception of the distal tip region that shows some double margin formation and ventral overgrowth.

When expressed via Scer\GAL4hs.PB in embryos, suppression of neuronal differentiation occurs due to excessive N activity. When expressed via Scer\GAL4e22c, animals die at approximately the first larval instar but with no gross morphological abnormalities.

Scer\GAL4sca-537.4-mediated expression (causing activation of N) leads to loss of R8 cells (inhibition R8 cell differentiation) and of other photoreceptor cells with incomplete penetrance.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Suppressor of
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has posterior notopleural bristle & trichogen cell phenotype, enhanceable by Df(3R)Dl-M2

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has anterior supraalar bristle & trichogen cell phenotype, enhanceable by Df(3R)Dl-M2

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has posterior supraalar bristle & trichogen cell phenotype, enhanceable by Df(3R)Dl-M2

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has anterior postalar bristle & trichogen cell phenotype, enhanceable by Df(3R)Dl-M2

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has anterior scutellar bristle & trichogen cell phenotype, enhanceable by Df(3R)Dl-M2

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has posterior scutellar bristle & trichogen cell phenotype, enhanceable by Df(3R)Dl-M2

Suppressed by
Statement
Reference

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has posterior scutellar bristle & trichogen cell phenotype, suppressible by Scer\GAL4l(3)31-1-31-1/fngUAS.cKa

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has presutural bristle & trichogen cell phenotype, suppressible by Tp(3;3)bxd110

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has anterior notopleural bristle & trichogen cell phenotype, suppressible by Tp(3;3)bxd110

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has posterior notopleural bristle & trichogen cell phenotype, suppressible by Tp(3;3)bxd110

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has anterior supraalar bristle & trichogen cell phenotype, suppressible by Tp(3;3)bxd110

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has posterior supraalar bristle & trichogen cell phenotype, suppressible by Tp(3;3)bxd110

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has anterior postalar bristle & trichogen cell phenotype, suppressible by Tp(3;3)bxd110

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has presutural bristle & trichogen cell phenotype, suppressible by Scer\GAL4l(3)31-1-31-1/fngUAS.cKa

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has posterior postalar bristle & trichogen cell phenotype, suppressible by Tp(3;3)bxd110

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has anterior dorsocentral bristle & trichogen cell phenotype, suppressible by Tp(3;3)bxd110

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has posterior dorsocentral bristle & trichogen cell phenotype, suppressible by Tp(3;3)bxd110

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has anterior scutellar bristle & trichogen cell phenotype, suppressible by Tp(3;3)bxd110

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has posterior scutellar bristle & trichogen cell phenotype, suppressible by Tp(3;3)bxd110

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has posterior notopleural bristle & trichogen cell phenotype, suppressible by Scer\GAL4l(3)31-1-31-1/fngUAS.cKa

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has anterior supraalar bristle & trichogen cell phenotype, suppressible by Scer\GAL4l(3)31-1-31-1/fngUAS.cKa

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has posterior supraalar bristle & trichogen cell phenotype, suppressible by Scer\GAL4l(3)31-1-31-1/fngUAS.cKa

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has anterior postalar bristle & trichogen cell phenotype, suppressible by Scer\GAL4l(3)31-1-31-1/fngUAS.cKa

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has anterior dorsocentral bristle & trichogen cell phenotype, suppressible by Scer\GAL4l(3)31-1-31-1/fngUAS.cKa

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has posterior dorsocentral bristle & trichogen cell phenotype, suppressible by Scer\GAL4l(3)31-1-31-1/fngUAS.cKa

Scer\GAL4l(3)31-1-31-1, SerUAS.cGa has anterior scutellar bristle & trichogen cell phenotype, suppressible by Scer\GAL4l(3)31-1-31-1/fngUAS.cKa

Enhancer of
Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

DlScer\UAS.cLa, SerScer\UAS.cGa double-mutant third instar nota clones (under the regulation of Scer\GAL4mat.αTub67C.T:Hsim\VP16) typically contain ten or more ectopic sensory organ precursors. Addition of the SerScer\UAS.cGa transgene, to DlRevF10 SerRX106 double mutant third instar nota clones, under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16, partially rescues the ectopic SOP phenotype, with approximately 26% of SOP positions exhibiting between one and four SOPs.

Weakly suppresses the wing nicking phenotype seen in mamN.Scer\UAS, Scer\GAL4C96 flies.

The transformation of the trichogen to a tormogen fate in the notal macrochaetae caused by SerScer\UAS.cGa expressed under the control of Scer\GAL4l(3)31-1-31-1 is enhanced by Df(3R)Dl-M2 and suppressed by Tp(3;3)bxd110. The bristle transformation phenotype seen when both SerScer\UAS.cGa and DlScer\UAS.cJa are expressed under the control of Scer\GAL4l(3)31-1-31-1 appears to be additive. The transformation of the trichogen to a tormogen fate in the notal macrochaetae caused by SerScer\UAS.cGa expressed under the control of Scer\GAL4l(3)31-1-31-1 is suppressed by co-expression of fngScer\UAS.cKa.

Expression in a fng- background causes dorsal cell proliferation (displacing the dorsal/ventral margin). Scer\GAL4hs.PB-mediated expression causes death prior to the end of embryogenesis. Mutants display severely reduced neuronal differentiation. Simultaneous expression with fngScer\UAS.cKa reduces lethality associated with ectopic expression of Ser and restores normal neuronal differentiation, flies develop into phenotypically wild type adults.

When expressed via Scer\GAL4hs.PB in a Df(3R)Dl-BX6 background, neuronal hyperplasia is reduced.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
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Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
SerScer\UAS.cGa
SerUAS.cGa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Gu
Secondary FlyBase IDs
    References (15)