FlyBase Allele Report: Dmel\Tom[UAS.cLa]

General Information

Symbol

Dmel\Tom^UAS.cLa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Lai

FlyBase ID

FBal0103930

Feature type

allele

Associated gene

Dmel\Tom

Associated Insertion(s)

Carried in Construct

P{UAS-Tom.L}

Also Known As

UAS-Tom

Key Links

Transgenic product class

wild_type

(Lai et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Lai et al., 2000)

Mutagen

in vitro construct

(Lai et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-Tom.L}

Cytology

Description

UASt regulatory sequences drive expression of the coding region and a few nucleotides of 5' UTR sequence (to provide translational initiation context) of Tom.

(Lai et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tom

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^insc-Mz1407

(Song and Lu, 2012)

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^elav-C155

(Ulvklo et al., 2012)

lethal, with Scer\GAL4^sca-537.4

(Lai et al., 2000)

visible, with Scer\GAL4^69B

(Lai et al., 2000)

visible, with Scer\GAL4^Bx-MS1096

(Lai et al., 2000)

visible, with Scer\GAL4^GMR.PF

(Lai et al., 2000)

visible, with Scer\GAL4^pnr-MD237

(Hamel et al., 2010)

Phenotype Manifest In

apterous-expressing neuron of the lateral cluster of the thorax, with Scer\GAL4^elav-C155

(Ulvklo et al., 2012)

chaeta | increased number, with Scer\GAL4^pnr-MD237

(Hamel et al., 2010)

external sensory organ precursor cell & dorsal mesothoracic disc, with Scer\GAL4^sca-537.4

(Lai et al., 2000)

eye, with Scer\GAL4^GMR.PF

(Lai et al., 2000)

eye photoreceptor cell, with Scer\GAL4^GMR.PF

(Lai et al., 2000)

intermediate neuronal progenitor, with Scer\GAL4^insc-Mz1407

(Song and Lu, 2012)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Lai et al., 2000)

leg sensillum, with Scer\GAL4^sca-537.4

(Lai et al., 2000)

macrochaeta, with Scer\GAL4^sca-537.4

(Lai et al., 2000)

microchaeta, with Scer\GAL4^sca-537.4

(Lai et al., 2000)

microchaeta & leg, with Scer\GAL4^sca-537.4

(Lai et al., 2000)

ommatidium, with Scer\GAL4^GMR.PF

(Lai et al., 2000)

sensory mother cell | increased number, with Scer\GAL4^neur-P72

(Bardin and Schweisguth, 2006)

sensory neuron | increased number, with Scer\GAL4^neur-P72

(Bardin and Schweisguth, 2006)

wing margin bristle, with Scer\GAL4^69B

(Lai et al., 2000)

wing margin bristle, with Scer\GAL4^Bx-MS1096

(Lai et al., 2000)

wing sensillum & wing vein, with Scer\GAL4^69B

(Lai et al., 2000)

wing sensillum & wing vein, with Scer\GAL4^Bx-MS1096

(Lai et al., 2000)

Detailed Description

Statement

Reference

Overexpression of Tom^Scer\UAS.cLa in neuroblasts under the control of Scer\GAL4^insc-Mz1407 leads to a complete depletion of type II neuroblasts.

(Song and Lu, 2012)

Embryos expressing Tom^Scer\UAS.cLa under the control of Scer\GAL4^elav-C155 have an increased number of "Ap" neurons in the NB5-6T lineage.

(Ulvklo et al., 2012)

Expression of Tom^Scer\UAS.cLa under the control of Scer\GAL4^pnr-MD237 post-embryonically (animals are transferred to 28[o]C after embryogenesis to inactivate Scer\GAL80^ts.αTub84B which is present in the stock) results in a strong neurogenic phenotype with an excess of bristles.

(Hamel et al., 2010)

In Tom^Scer\UAS.cLa ; Scer\GAL4^neur-P72 animals have extra sensory organ precursors and neurons are specified at the expense of other cell-yypes in the sensory organ lineage.

No effect on the specification of wing margin cells or wing pouch growth is seen in Tom^Scer\UAS.cLa animals carrying Scer\GAL4^ptc-559.1, Scer\GAL4^dpp.PS or Scer\GAL4^Ser.PGF.

(Bardin and Schweisguth, 2006)

Flies expressing Tom^Scer\UAS.cLa under the control of Scer\GAL4^GMR.PF have strongly roughened eyes with defects that include tufting of interommatidial bristles and excess photoreceptors.

Expression of Tom^Scer\UAS.cLa under the control of either Scer\GAL4^Bx-MS1096 or Scer\GAL4^69B does not appear to alter wing vein fates or the overall integrity of the wing margin, although sensory organ fates in each of these territories are altered.

Supernumerary sensory organ precursor cells are seen in wing imaginal discs of animals expressing Tom^Scer\UAS.cLa under the control of Scer\GAL4^sca-537.4.

(Lai et al., 2000)

Expression of Tom^Scer\UAS.cLa under the control of Scer\GAL4^sca-537.4 results in a high percentage of lethality at late pupal/pharate adult stages. Adult escapers show strong tufting of nearly all macrochaetae and microchaetae, especially on the legs. Flies expressing Tom^Scer\UAS.cLa under the control of Scer\GAL4^GMR.PF have strongly roughened eyes with defects that include tufting of interommatidial bristles and excess photoreceptors. Expression of Tom^Scer\UAS.cLa under the control of either Scer\GAL4^Bx-MS1096 or Scer\GAL4^69B does not appear to alter wing vein fates or the overall integrity of the wing margin, although sensory organ fates in each of these territories are altered. Supernumerary sensory organ precursor cells are seen in wing imaginal discs of animals expressing Tom^Scer\UAS.cLa under the control of Scer\GAL4^sca-537.4.

(Zaffran and Frasch, 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^pnr-MD237, Tom^UAS.cLa has visible phenotype, suppressible by α4GT1^GS2078, Scer\GAL4^pnr-MD237

(Hamel et al., 2010)

Enhancer of

Statement

Reference

Tom^UAS.cLa/Scer\GAL4^ey.200.Exel is an enhancer of decreased size | adult stage phenotype of Scer\GAL4^ey.200.Exel, fng^UAS.cKa

(Lee et al., 2025)

Tom^UAS.cLa/Scer\GAL4^ey.200.Exel is an enhancer of decreased size | larval stage phenotype of Scer\GAL4^ey.200.Exel, fng^UAS.cKa

(Lee et al., 2025)

Other

Statement

Reference

Df(3R)ED5296/+, Scer\GAL4^insc.PU, Tom^UAS.cLa has abnormal neuroanatomy | embryonic stage phenotype

(Bivik et al., 2016)

Df(3R)ED5296/+, Scer\GAL4^insc.PU, Tom^UAS.cLa has increased cell number | embryonic stage phenotype

(Bivik et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^pnr-MD237, Tom^UAS.cLa has chaeta | increased number phenotype, suppressible by α4GT1^GS2078, Scer\GAL4^pnr-MD237

(Hamel et al., 2010)

Enhancer of

Statement

Reference

Tom^UAS.cLa/Scer\GAL4^ey.200.Exel is an enhancer of eye phenotype of Scer\GAL4^ey.200.Exel, fng^UAS.cKa

(Lee et al., 2025)

Tom^UAS.cLa/Scer\GAL4^ey.200.Exel is an enhancer of eye disc | larval stage phenotype of Scer\GAL4^ey.200.Exel, fng^UAS.cKa

(Lee et al., 2025)

Suppressor of

Statement

Reference

Tom^UAS.cLa is a suppressor of wing margin phenotype of Scer\GAL4^Ser.PGF, neur^C701S.UAS

(Bardin and Schweisguth, 2006)

NOT Suppressor of

Statement

Reference

Tom^UAS.cLa is a non-suppressor of wing margin phenotype of Scer\GAL4^Ser.PGF, mib1^C1205S.UAS

(Bardin and Schweisguth, 2006)

Other

Statement

Reference

Df(3R)ED5296/+, Scer\GAL4^insc.PU, Tom^UAS.cLa has apterous-expressing neuron of the lateral cluster of the thorax | increased number | embryonic stage phenotype

(Bivik et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Tom^Scer\UAS.cLa under the control of Scer\GAL4^insc.PU in Df(3R)ED5296/+ embryos results in a significant increase in the number of Ap neurons, as compared with controls.

(Bivik et al., 2016)

The excess bristles phenotype caused by expression of Tom^Scer\UAS.cLa under the control of Scer\GAL4^pnr-MD237 post-embryonically (animals are transferred to 28[o]C after embryogenesis to inactivate Scer\GAL80^ts.αTub84B which is present in the stock) is suppressed by co-expression of α4GT1^GS2078.

(Hamel et al., 2010)

The inhibition of wing margin formation due to neur^{C701S.Scer\UAS}; Scer\GAL4^Ser.PGF is suppressed by Tom^Scer\UAS.cLa. However, the inhibition of wing margin formation due to mib1^{C1205S.Scer\UAS}; Scer\GAL4^Ser.PGF is not.

(Bardin and Schweisguth, 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26685

w¹¹¹⁸; P{UAS-Tom.L}2 P{UAS-Tom.L}3/TM6C, Sb¹ Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Tom^Scer\UAS.cLa

Tom^UAS.cLa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Lai

Secondary FlyBase IDs

References (13)

Report Sections

Open Close