FlyBase Allele Report: Dmel\ap[UGO35]

General Information

Symbol

Dmel\ap^UGO35

Species

D. melanogaster

Name

FlyBase ID

FBal0030502

Feature type

allele

Associated gene

Dmel\ap

Associated Insertion(s)

Carried in Construct

Also Known As

ap^UG035

Key Links

Allele class

loss of function allele

amorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Ng et al., 1996, Couso et al., 1995, Lundgren et al., 1995, Diaz-Benjumea and Cohen, 1993, Cohen et al., 1992)

loss of function allele

(Klein et al., 1998)

Mutagen

Progenitor genotype

ap^rK568

(Cohen et al., 1992)

Cytology

Description

Small deletion removing first exon of the ap gene.

(Diaz-Benjumea and Cohen, 1993)

P element has been imprecisely excised from the gene resulting in a deletion of 6 kb removing the first exon of the ap transcription unit.

(Cohen et al., 1992)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | embryonic stage 16

(Bertin et al., 2021)

lethal (with ap^md544)

(Rincon-Limas et al., 2000)

short lived (with ap^md544)

(Rincon-Limas et al., 2000, Rincon-Limas et al., 1999)

sterile (with ap^md544)

(Rincon-Limas et al., 2000, Rincon-Limas et al., 1999)

visible

(Gohl et al., 2008, Klein et al., 1998)

visible (with ap^md544)

(Milan et al., 2001, Weihe et al., 2001, Milan and Cohen, 1999, Rincon-Limas et al., 1999)

visible (with ap^MM-Mcp)

(Gohl et al., 2008)

visible (with ap^rK568)

(Bieli et al., 2015)

visible (with Df(2R)DG)

(Gohl et al., 2008)

visible (with Df(2R)DG-Mcp)

(Gohl et al., 2008)

visible (with Df(2R)nap1)

(Gohl et al., 2008)

visible | recessive

(Klein and Martinez Arias, 1998, Diaz-Benjumea and Cohen, 1993, Cohen et al., 1992)

Phenotype Manifest In

A1-7 lateral transverse muscle cell

(Müller et al., 2010)

dorsal-ventral compartment boundary of the wing disc (with ap^md544)

(Milan et al., 2001)

haltere

(Cohen et al., 1992)

haltere (with ap^md544)

(Rincon-Limas et al., 1999)

lateral transverse muscle cell | decreased number | embryonic stage 16

(Bertin et al., 2021)

lateral transverse muscle cell | embryonic stage 16

(Bertin et al., 2021)

lateral transverse muscle cell | embryonic stage 16 | increased number

(Bertin et al., 2021)

macrochaeta & thorax | dorsal

(Cohen et al., 1992)

ovary (with ap^md544)

(Rincon-Limas et al., 2000)

pretarsus (with ap^md544)

(Pueyo and Couso, 2004, Pueyo et al., 2000)

pretarsus (with Df(2R)nap1)

(Pueyo et al., 2000)

scutellum (with ap^md544)

(Rincon-Limas et al., 1999)

tarsal segment 4 (with ap^md544)

(Pueyo and Couso, 2004, Pueyo et al., 2000)

tarsal segment 4 (with Df(2R)nap1)

(Pueyo et al., 2000)

wing

(Gohl et al., 2008, Klein and Martinez Arias, 1998, Klein and Martinez Arias, 1998, Klein et al., 1998, Cohen et al., 1992)

wing (with ap^md544)

(Milan et al., 2001, Weihe et al., 2001, Rincon-Limas et al., 2000, Milan and Cohen, 1999, Rincon-Limas et al., 1999)

wing (with ap^MM-Mcp)

(Gohl et al., 2008)

wing (with ap^rK568)

(Bieli et al., 2015)

wing (with Df(2R)DG)

(Gohl et al., 2008)

wing (with Df(2R)DG-Mcp)

(Gohl et al., 2008)

wing (with Df(2R)nap1)

(Gohl et al., 2008)

wing disc

(Villa-Cuesta and Modolell, 2005, Klein et al., 2000)

wing disc (with ap^md544)

(Milan and Cohen, 2003, Milan and Cohen, 1999)

wing disc (with ap^rK568)

(Koelzer and Klein, 2006)

wing disc dorsal compartment | somatic clone

(Diaz-Benjumea and Cohen, 1993)

wing margin (with ap^md544)

(Milan et al., 2001)

wing margin bristle (with ap^md544)

(Milan and Cohen, 1999)

Detailed Description

Statement

Reference

ap^UGO35 stage 16 embryos show lateral transverse (LT) muscle defects, namely missing and split muscles.

(Bertin et al., 2021)

ap^UGO35/ap^rK568 mutant wing discs have no wing pouch and the adult flies do not develop any wings.

(Bieli et al., 2015)

ap^UGO35/+ flies have normal wings.

(Bronstein et al., 2010)

Mutants show occasional losses and misarrangements of individual embryonic abdominal lateral transverse muscles.

(Müller et al., 2010)

Homozygous ap^UGO35 mutant flies lack wings.

ap^UGO35/Df(2R)nap1 transheterozygotes completely lack wings.

Wing defects are seen in ap^{loxP.MCP-PRE.MM}/ap^UGO35 mutant flies, but they are significantly less severe than those seen in ap^{loxP.MCP-PRE.MM} homozygotes.

Very mild wing defects are seen in Df(2R)DG/ap^UGO35 mutant flies, but they are significantly less severe than those seen in Df(2R)DG homozygotes.

Very few wing defects are seen in ap^f00451/ap^UGO35 mutant flies.

While both homozygotes completely lack wings, the majority of ap^UGO35/Df(2R)DG-Mcp transheterozygotes have crumpled wings.

(Gohl et al., 2008)

The dorsal/ventral boundary fails to form correctly in ap^UGO35/ap^rK568 mutant wing discs.

(Koelzer and Klein, 2006)

Wing discs homozygous for ap^UGO35 have in general a profoundly altered morphology.

(Villa-Cuesta and Modolell, 2005)

In ap^UGO35/ap^md544 the fourth tarsal segment is almost completely lost and is fused to the fifth. Other segments are normal.

(Pueyo and Couso, 2004)

ap^md544/ap^UGO35 wing discs do not form a smooth boundary between dorsal and ventral cells.

(Milan et al., 2001)

The wing margin is reduced and the wing is considerably smaller than normal in ap^md544/ap^UGO35 flies. When present, wing margin bristles have ventral identity.

(Milan et al., 2001)

ap^md544/ap^UGO35 fail to develop normal wings.

(Weihe et al., 2001)

Wing pouch is missing.

(Klein et al., 2000)

Transheterozygotes with Df(2R)nap1 show fusion of tarsus 4 with 5, reduction and deformity or loss of tarsus 4. Tarsus four and five fusions appear with lower frequency and expressivity in ap^UGO35/ap^md544 transheterozygotes.

(Pueyo et al., 2000)

ap^md544/ap^UGO35 flies have immature ovaries, are sterile, die within 24-72 hours of eclosion and have no wings.

(Rincon-Limas et al., 2000)

ap^md544/ap^UGO35 flies show reduction of the wing and complete loss of wing margin. ap^md544/ap^UGO35 wing discs have small wing pouches.

(Milan and Cohen, 1999)

ap^md544/ap^UGO35 flies lack wings, halteres and the scutellum region of the notum. The flies are sterile and have a lifespan of 1-3 days after eclosion.

(Rincon-Limas et al., 1999)

The wing blade does not develop in homozygous flies.

(Klein and Martinez Arias, 1998)

Stumps are present in place of the wing.

(Klein et al., 1998)

Neurons of homozygous embryos carrying the P{apC-tau-lacZ} exhibit pathfinding and fasciculation defects: ap neurons that project their axons along abnormal pathways and fail to fasciculate with one another. The ap neurons still project anteriorly within the connectives.

(Lundgren et al., 1995)

Clonal analysis, in the wing disc, shows that clones in the dorsal compartment that lack ap function show a cell-autonomous transformation to a ventral identity, and the fate of wild type dorsal cells is altered as a consequence of their proximity to the ap^- cells in the clone.

(Diaz-Benjumea and Cohen, 1993)

Wings and halteres absent. Homozygous ap^UGO35 flies do not express the ap transcript in the embryo, in the larval brain, or in the imaginal discs. Lack some dorsal thoracic bristles.

(Cohen et al., 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

ap^UGO35/Df(2R)DG has visible phenotype, enhanceable by z^a

(Gohl et al., 2008)

NOT Enhanced by

Statement

Reference

ap^UGO35/Df(2R)DG has visible phenotype, non-enhanceable by z^a

(Gohl et al., 2008)

Suppressed by

Statement

Reference

ap^md544/ap^UGO35 has visible phenotype, suppressible | partially by fng^EP3082/Scer\GAL4^ap-md544

(Milan et al., 2001)

ap^md544/ap^UGO35 has visible phenotype, suppressible | partially by Scer\GAL4^ap-md544/Dr^UAS.cIa

(Milan et al., 2001)

ap^md544/ap^UGO35 has visible phenotype, suppressible by Scer\GAL4^ap-md544/ap::Bx^{dLAp.UAS.Tag:FLAG}

(Weihe et al., 2001)

ap^md544/ap^UGO35 has visible phenotype, suppressible by Scer\GAL4^ap-md544/Ser^UAS.cSa

(Milan and Cohen, 1999)

ap^md544/ap^UGO35 has visible phenotype, suppressible by fng^EP3082/Scer\GAL4^ap-md544

(Milan and Cohen, 1999)

ap^md544/ap^UGO35 has visible phenotype, suppressible by Scer\GAL4^ap-md544/Hsap\LHX2^UAS.cRa

(Rincon-Limas et al., 1999)

ap^md544/ap^UGO35 has sterile phenotype, suppressible by Scer\GAL4^ap-md544/Hsap\LHX2^UAS.cRa

(Rincon-Limas et al., 1999)

ap^md544/ap^UGO35 has short lived phenotype, suppressible by Scer\GAL4^ap-md544/Hsap\LHX2^UAS.cRa

(Rincon-Limas et al., 1999)

ap^UGO35 has visible | recessive phenotype, suppressible by Scer\GAL4^dpp.blk1/Ser^UAS.cSa

(Klein and Martinez Arias, 1998)

ap^UGO35 has visible | recessive phenotype, suppressible by Scer\GAL4^dpp.blk1/fng^UAS.cKa

(Klein and Martinez Arias, 1998)

ap^UGO35 has visible | recessive phenotype, suppressible by Delta^UAS.cHa/Scer\GAL4^dpp.blk1

(Klein and Martinez Arias, 1998)

Enhancer of

Statement

Reference

ap[+]/ap^UGO35 is an enhancer of visible | dominant phenotype of Bx¹

(Bejarano et al., 2008, Milan et al., 2004)

ap^UGO35 is an enhancer of visible | dominant phenotype of Bx¹

(Milan et al., 1998)

Suppressor of

Statement

Reference

ap^UGO35/ap^UGO35 is a suppressor | partially of visible phenotype of Scer\GAL4^dpp.blk1, Ser^UAS.cSa

(Klein and Martinez Arias, 1998)

Other

Statement

Reference

Scer\GAL4^ap-md544, Ser^UAS.cSa, ap^md544/ap^UGO35, fng^EP3082 has visible phenotype

(Milan and Cohen, 1999)

Hsap\LHX2^UAS.cRa, Scer\GAL4^ap-md544, ap^md544/ap^UGO35 has visible phenotype

(Rincon-Limas et al., 1999)

Hsap\LHX2^UAS.cRa, Scer\GAL4^ap-md544, ap^UGO35 has visible | recessive phenotype

(Rincon-Limas et al., 1999)

Scer\GAL4^dpp.blk1, Ser^UAS.cSa, ap^UGO35 has visible phenotype

(Klein et al., 1998)

Scer\GAL4^dpp.blk1, ap^UGO35, fng^UAS.cKa has visible phenotype

(Klein et al., 1998)

Phenotype Manifest In

Enhanced by

Statement

Reference

ap^UGO35/Df(2R)DG has wing phenotype, enhanceable by z^a

(Gohl et al., 2008)

NOT Enhanced by

Statement

Reference

ap^UGO35/Df(2R)DG has wing phenotype, non-enhanceable by z^a

(Gohl et al., 2008)

Suppressed by

Statement

Reference

ap^md544/ap^UGO35 has tarsal segment 4 phenotype, suppressible by Scer\GAL4^ap-md544/ap::Lim3^UAS.cOa

(Pueyo and Couso, 2004)

ap^md544/ap^UGO35 has pretarsus phenotype, suppressible by Scer\GAL4^ap-md544/ap::Chi^{ΔLID.ΔLIM.UAS.Tag:MYC}

(Pueyo and Couso, 2004)

ap^md544/ap^UGO35 has pretarsus phenotype, suppressible by Scer\GAL4^ap-md544/ap::Lim3^UAS.cOa

(Pueyo and Couso, 2004)

ap^md544/ap^UGO35 has tarsal segment 4 phenotype, suppressible by Scer\GAL4^ap-md544/ap::Chi^{ΔLID.ΔLIM.UAS.Tag:MYC}

(Pueyo and Couso, 2004)

ap^md544/ap^UGO35 has wing margin phenotype, suppressible | partially by Scer\GAL4^ap-md544/Dr^UAS.cIa

(Milan et al., 2001)

ap^md544/ap^UGO35 has wing margin phenotype, suppressible | partially by fng^EP3082/Scer\GAL4^ap-md544

(Milan et al., 2001)

ap^md544/ap^UGO35 has wing phenotype, suppressible | partially by fng^EP3082/Scer\GAL4^ap-md544

(Milan et al., 2001)

ap^md544/ap^UGO35 has wing phenotype, suppressible by Scer\GAL4^ap-md544/ap::Bx^{dLAp.UAS.Tag:FLAG}

(Weihe et al., 2001)

ap^UGO35 has wing disc phenotype, suppressible by H^E31

(Klein et al., 2000)

ap^md544/ap^UGO35 has wing phenotype, suppressible by Scer\GAL4^ap-md544/Ser^UAS.cSa

(Milan and Cohen, 1999)

ap^md544/ap^UGO35 has wing margin bristle phenotype, suppressible by Scer\GAL4^ap-md544/Ser^UAS.cSa

(Milan and Cohen, 1999)

ap^md544/ap^UGO35 has wing phenotype, suppressible by fng^EP3082/Scer\GAL4^ap-md544

(Milan and Cohen, 1999)

ap^md544/ap^UGO35 has wing margin bristle phenotype, suppressible by fng^EP3082/Scer\GAL4^ap-md544

(Milan and Cohen, 1999)

ap^md544/ap^UGO35 has wing phenotype, suppressible by Scer\GAL4^ap-md544/Hsap\LHX2^UAS.cRa

(Rincon-Limas et al., 1999)

ap^md544/ap^UGO35 has haltere phenotype, suppressible by Scer\GAL4^ap-md544/Hsap\LHX2^UAS.cRa

(Rincon-Limas et al., 1999)

ap^md544/ap^UGO35 has scutellum phenotype, suppressible by Scer\GAL4^ap-md544/Hsap\LHX2^UAS.cRa

(Rincon-Limas et al., 1999)

ap^UGO35 has wing phenotype, suppressible by Scer\GAL4^dpp.blk1/vg^UAS.cKa

(Klein and Martinez Arias, 1998)

ap^UGO35 has wing phenotype, suppressible by Scer\GAL4^dpp.blk1/Ser^UAS.cSa

(Klein and Martinez Arias, 1998)

ap^UGO35 has wing phenotype, suppressible by Scer\GAL4^dpp.blk1/fng^UAS.cKa

(Klein and Martinez Arias, 1998)

ap^UGO35 has wing phenotype, suppressible by Delta^UAS.cHa/Scer\GAL4^dpp.blk1

(Klein and Martinez Arias, 1998)

NOT suppressed by

Statement

Reference

ap^rK568/ap^UGO35 has wing disc phenotype, non-suppressible by H^E31/H^E31

(Koelzer and Klein, 2006)

ap^rK568/ap^UGO35 has wing disc phenotype, non-suppressible by Su(H)⁸/Su(H)^del47

(Koelzer and Klein, 2006)

ap^md544/ap^UGO35 has wing disc phenotype, non-suppressible by fng^{UAS.B.Tag:MYC}/Scer\GAL4^ap-md544

(Milan and Cohen, 2003)

ap^md544/ap^UGO35 has wing disc phenotype, non-suppressible by fng^EP3082/Scer\GAL4^ap-md544

(Milan and Cohen, 2003)

ap^md544/ap^UGO35 has dorsal-ventral compartment boundary of the wing disc phenotype, non-suppressible by Scer\GAL4^ap-md544/caps^UAS.cSa

(Milan et al., 2001)

ap^md544/ap^UGO35 has dorsal-ventral compartment boundary of the wing disc phenotype, non-suppressible by Scer\GAL4^ap-md544/Con^UAS.cRa

(Milan et al., 2001)

ap^md544/ap^UGO35 has dorsal-ventral compartment boundary of the wing disc phenotype, non-suppressible by fng^EP3082/Scer\GAL4^ap-md544

(Milan et al., 2001)

ap^md544/ap^UGO35 has wing phenotype, non-suppressible by Scer\GAL4^ap-md544/Dr^UAS.cIa

(Milan et al., 2001)

ap^md544/ap^UGO35 has pretarsus phenotype, non-suppressible by Chi^{FL.UAS.Tag:MYC}/Scer\GAL4^30A

(Pueyo et al., 2000)

ap^md544/ap^UGO35 has tarsal segment 4 phenotype, non-suppressible by Chi^{FL.UAS.Tag:MYC}/Scer\GAL4^30A

(Pueyo et al., 2000)

ap^UGO35 has wing phenotype, non-suppressible by wg^UAS.cGa/Scer\GAL4^dpp.blk1

(Klein and Martinez Arias, 1998)

Enhancer of

Statement

Reference

ap[+]/ap^UGO35 is an enhancer of wing margin phenotype of Bx²

(Bronstein et al., 2010)

ap[+]/ap^UGO35 is an enhancer of wing margin phenotype of Bx¹

(Bejarano et al., 2008, Milan et al., 2004)

ap[+]/ap^UGO35 is an enhancer of pretarsus phenotype of Bar^M2

(Pueyo and Couso, 2004)

ap[+]/ap^UGO35 is an enhancer of tarsal segment 4 phenotype of Bar^M2

(Pueyo and Couso, 2004)

ap^UGO35 is an enhancer of wing phenotype of Bx¹

(Milan et al., 1998)

Suppressor of

Statement

Reference

ap^UGO35 is a suppressor of scutellar bristle | ectopic phenotype of Chi^E

(Ramain et al., 2000)

ap^UGO35 is a suppressor of thorax phenotype of Chi^E

(Ramain et al., 2000)

ap^UGO35/ap^UGO35 is a suppressor | partially of wing phenotype of Scer\GAL4^dpp.blk1, Ser^UAS.cSa

(Klein and Martinez Arias, 1998)

Other

Statement

Reference

ap^UGO35, lms^S95 has A1-7 lateral transverse muscle cell | embryonic stage 16 phenotype

(Müller et al., 2010)

Scer\GAL4^ap-md544, ap^md544/ap^UGO35, fng^EP3082 has wing vein | dorsal phenotype

(Milan et al., 2001)

Scer\GAL4^ap-md544, ap^md544/ap^UGO35, fng^EP3082 has alar lobe | dorsal phenotype

(Milan et al., 2001)

Scer\GAL4^ap-md544, ap^md544/ap^UGO35, fng^EP3082 has wing margin | anterior | dorsal phenotype

(Milan et al., 2001)

H^E31, ap^UGO35 has wing margin phenotype

(Klein et al., 2000)

Scer\GAL4^ap-md544, ap^md544/ap^UGO35, fng^EP3082 has wing disc phenotype

(Milan and Cohen, 1999)

Scer\GAL4^ap-md544, ap^md544/ap^UGO35, fng^EP3082 has wing margin bristle | ectopic phenotype

(Milan and Cohen, 1999)

Scer\GAL4^ap-md544, Ser^UAS.cSa, ap^md544/ap^UGO35, fng^EP3082 has wing phenotype

(Milan and Cohen, 1999)

Scer\GAL4^ap-md544, Ser^UAS.cSa, ap^md544/ap^UGO35 has wing disc phenotype

(Milan and Cohen, 1999)

Scer\GAL4^ap-md544, Ser^UAS.cSa, ap^md544/ap^UGO35 has wing margin bristle | ectopic phenotype

(Milan and Cohen, 1999)

Hsap\LHX2^UAS.cRa, Scer\GAL4^ap-md544, ap^md544/ap^UGO35 has scutellar bristle phenotype

(Rincon-Limas et al., 1999)

Scer\GAL4^dpp.blk1, ap^UGO35, fng^UAS.cKa has wing | ectopic phenotype

(Klein et al., 1998)

Scer\GAL4^dpp.blk1, Ser^UAS.cSa, ap^UGO35 has wing | ectopic phenotype

(Klein et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

In lms^S95, ap^UGO35 double mutant embryos most of the lateral transverse (LT) muscles form, though the number of hemisegments with only three or two LT muscles present is significantly higher than for each of the single mutants. These effects appear additive rather than interactive.

(Müller et al., 2010)

z¹ does not enhance the mild wing phenotypes seen in ap^UGO35/Df(2R)DG mutant flies.

z^a slightly enhances the mild wing phenotypes in ap^UGO35/Df(2R)DG mutant flies.

(Gohl et al., 2008)

ap^UGO35/ap^rK568; H^E31 and ap^UGO35/ap^rK568; Su(H)^del47/Su(H)⁸ wing discs fail to form a dorsal/ventral compartment boundary.

(Koelzer and Klein, 2006)

The severity of the Bx¹/+ wing scalloping phenotype is enhanced by one copy of ap^UGO35.

(Milan et al., 2004)

ap^UGO35/+ does not cause any visible leg phenotypes in combination with Chi^g371/+, Chi^g96.1/+ or Chi^e5.5/+. ap::Chi^{ΔLID.ΔLIM.Scer\UAS.T:Hsap\MYC}; Scer\GAL4^ap-md544 suppresses fusion of tarsal segments 4 and 5 in ap^UGO35/ap^md544 flies. ap::Lim3^Scer\UAS.cOa; Scer\GAL4^ap-md544 suppresses fusion of tarsal segments 4 and 5 in ap^UGO35/ap^md544 flies. B^M2/+ enhances the tarsal phenotype of ap^UGO35/ap^md544 flies: tarsal segment 4 is completely absent and the joint between tarsal segments 3 and 5 is affected. The tarsal segment phenotype of B^M2 hemizygous males is enhanced by ap^UGO35/+: in the resulting flies tarsal segments 4 and 5 are shorter and are fused in 61% of the legs.

(Pueyo and Couso, 2004)

Expression of caps^Scer\UAS.cSa under the control of Scer\GAL4^ap-md544 restores the smooth interface between dorsal and ventral cells in ap^md544/ap^UGO35 wing discs. The boundary between the dorsal and ventral cells is still irregular in ap^md544/ap^UGO35 wing discs expressing trn^Scer\UAS.cMa under the control of Scer\GAL4^ap-md544. Expression of Con^Scer\UAS.cRa or fng^EP3082 under the control of Scer\GAL4^ap-md544 does not restore a smooth boundary between the dorsal and ventral cells in ap^md544/ap^UGO35 wing discs.

(Milan et al., 2001)

Expression of Dr^Scer\UAS.cIa under the control of Scer\GAL4^ap-md544 in ap^md544/ap^UGO35 flies does not restore outgrowth of the wing, although the few margin bristles seen in the dorsal surface of these wings have dorsal identity. Growth of the wing and wing margin formation can be restored in ap^md544/ap^UGO35 flies expressing fng^EP3082 under the control of Scer\GAL4^ap-md544. Both surfaces of the wing differentiate ventral structures; the anterior wing margin and alula differentiate ventral bristles on both surfaces and the pattern of vein corrugation is ventral. Co-expression of fng^EP3082 and Dr^Scer\UAS.cIa under the control of Scer\GAL4^ap-md544 in ap^md544/ap^UGO35 flies results in dorsal differentiation in bristles of the dorsal anterior wing margin.

(Milan et al., 2001)

ap^UGO35/ap^md544 flies expressing fng^Scer\UAS.cKa under the control of Scer\GAL4^ap-md544 do not have wing margin defects.

(O'Keefe and Thomas, 2001)

ap^UGO35,H^E31 double mutants have large wing pouches with no margin structures.

(Klein et al., 2000)

The addition of this mutant to Chi^E homozygotes suppresses the thoracic left and loss of bristle phenotype seen in these flies.

(Ramain et al., 2000)

Expression of Ser^Scer\UAS.cSa under the control of Scer\GAL4^ap-md544 in ap^md544/ap^UGO35 flies restores the wing margin and supports growth of the wing. Patches of ectopic wing margin are found in both surfaces of the rescued wings and overgrowth of the wing can be seen. Expression of fng^EP3082 under the control of Scer\GAL4^ap-md544 rescues the phenotype of ap^md544/ap^UGO35 flies. Patches of ectopic wing margin form and can be associated with outgrowth of the wing. Groups of cells appear to violate the dorsoventral boundary in ap^md544/ap^UGO35 wing discs which are also expressing Ser^Scer\UAS.cSa under the control of Scer\GAL4^ap-md544. The mispositioned patches of dorsal or ventral cells form relatively smooth interfaces with surrounding cells. Cells appear to violate the dorsoventral boundary in ap^md544/ap^UGO35 wing discs which are also expressing fng^EP3082 under the control of Scer\GAL4^ap-md544. Expression of both Ser^Scer\UAS.cSa and fng^EP3082 under the control of Scer\GAL4^ap-md544 in ap^md544/ap^UGO35 flies results in well-developed wings which differentiate ventral features on both surfaces; the wing margin, veins and sense organs on the topologically dorsal surface have exclusively ventral identity.

(Milan and Cohen, 1999)

Scer\GAL4^dpp.blk1-mediated expression of vg^Scer\UAS.cKa rescues loss of wing phenotype. Wing stem is displaced dorsally into the thorax. Scer\GAL4^dpp.blk1-mediated expression of wg^Scer\UAS.cGa does not rescue loss of wing tissue.

(Klein and Martinez Arias, 1998)

The wing phenotype of homozygous flies is partially rescued by Ser^Scer\UAS.cSa, fng^Scer\UAS.cKa or Dl^Scer\UAS.cHa expressed under the control of Scer\GAL4^dpp.blk1.

(Klein and Martinez Arias, 1998)

Scer\GAL4^dpp.blk1-mediated expression of fng^Scer\UAS.cKa elicits an ectopic wing. Wing exhibits DV asymmetries and the margin is on the dorsal side. Mediated expression of Ser^Scer\UAS.cSa elicits an ectopic wing. Wing consists of dorsal and ventral cells and the wing margin cells are on the dorsal side.

(Klein et al., 1998)

Xenogenetic Interactions

Statement

Reference

The ap^md544/ap^UGO35 phenotypes are rescued by Hsap\LHX2^Scer\UAS.cRa expressed under the control of Scer\GAL4^ap-md544. ap^md544/ap^UGO35 flies rescued by the expression of Hsap\LHX2^Scer\UAS.cRa under the control of Scer\GAL4^ap-md544 often develop one to two extra bristles in the scutellum.

(Rincon-Limas et al., 1999)

Complementation and Rescue Data

Fails to complement

ap⁴

(Gohl et al., 2008)

ap^56f

(Gohl et al., 2008)

ap^rK568

(Gohl et al., 2008)

Rescued by

ap^md544/ap^UGO35 is rescued by Scer\GAL4^ap-md544/ap^UAS.cRa

(Rincon-Limas et al., 2000)

ap^md544/ap^UGO35 is rescued by Scer\GAL4^ap-md544/ap^UAS.cRa

(Rincon-Limas et al., 1999)

Partially rescued by

ap^rK568/ap^UGO35 is partially rescued by ap^cDNA.E+P

(Bieli et al., 2015)

ap^rK568/ap^UGO35 is partially rescued by ap^cDNA.DV+E

(Bieli et al., 2015)

ap^rK568/ap^UGO35 is partially rescued by ap^cDNA.DV+E+P

(Bieli et al., 2015)

ap^md544/ap^UGO35 is partially rescued by Scer\GAL4^ap-md544/ap^L1.UAS

(Rincon-Limas et al., 2000)

Not rescued by

ap^rK568/ap^UGO35 is not rescued by ap^cDNA.DV+P

(Bieli et al., 2015)

Comments

Expression of two copies of ap^cDNA.DV+E partially rescues the wing loss seen in ap^UGO35/ap^rK568 mutant flies. The wing margin fails to form.

Expression of two copies of ap^cDNA.DV+E+P partially rescues the wing loss seen in ap^UGO35/ap^rK568 mutant flies. Wing rescue is not perfect: a clear dorsoventral margin is observed.

Expression of two copies of ap^cDNA.DV+P fails to rescue the wing loss seen in ap^UGO35/ap^rK568 mutant flies.

Expression of two copies of ap^cDNA.E+P partially rescues the wing loss seen in ap^UGO35/ap^rK568 mutant flies. The wing margin fails to form.

(Bieli et al., 2015)

Reduction and fusion of the fourth tarsal segment in ap^md544/Df(2R)nap1 animals is rescued by ap^Scer\UAS.cOa.

(Pueyo and Couso, 2004)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

ap^UG035

(Fisher et al., 2017, Koelzer and Klein, 2006, Wu and Cohen, 2002, Klein et al., 2000, Pueyo et al., 2000, Bachmann and Knust, 1998)

ap^UGO35

(Bertin et al., 2021, Inami et al., 2021, Bieli et al., 2015, Herrero et al., 2014, Bronstein et al., 2010, Müller et al., 2010, Gohl et al., 2008, Gohl et al., 2007, Herrero et al., 2007)

ap^null

(Inami et al., 2021)

ap^rv-UGO35

ap^ugo35

(Bejarano et al., 2008, Milan et al., 2004, Milan et al., 2001)

Name Synonyms

Secondary FlyBase IDs

FBal0030505

References (39)

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