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Citation
Fisher, Y.E., Yang, H.H., Isaacman-Beck, J., Xie, M., Gohl, D.M., Clandinin, T.R. (2017). FlpStop, a tool for conditional gene control in Drosophila.  eLife 6(): e22279.
FlyBase ID
FBrf0235034
Publication Type
Research paper
Abstract

Manipulating gene function cell type-specifically is a common experimental goal in Drosophila research and has been central to studies of neural development, circuit computation, and behavior. However, current cell type-specific gene disruption techniques in flies often reduce gene activity incompletely or rely on cell division. Here we describe FlpStop, a generalizable tool for conditional gene disruption and rescue in post-mitotic cells. In proof-of-principle experiments, we manipulated apterous, a regulator of wing development. Next, we produced conditional null alleles of Glutamic acid decarboxylase 1 (Gad1) and Resistant to dieldrin (Rdl), genes vital for GABAergic neurotransmission, as well as cacophony (cac) and paralytic (para), voltage-gated ion channels central to neuronal excitability. To demonstrate the utility of this approach, we manipulated cac in a specific visual interneuron type and discovered differential regulation of calcium signals across subcellular compartments. Thus, FlpStop will facilitate investigations into the interactions between genes, circuits, and computation.

PubMed ID
PubMed Central ID
PMC5342825 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    eLife
    Title
    eLife
    ISBN/ISSN
    2050-084X
    Data From Reference
    Aberrations (4)
    Alleles (28)
    Genes (12)
    Natural transposons (1)
    Insertions (23)
    Experimental Tools (2)
    Transgenic Constructs (6)