mesothoracic tergum & chaeta | supernumerary, with Scer\GAL4sca-C253
Distal wing disc clones expressing vgScer\UAS.cKa under the control of Scer\GAL4tub.PU in a l(2)gl4 mutant background show rapid elimination and are rarely recovered. Proximal clones are more often recovered but also undergo cell death and basal extrusion. No neoplastic transformation is seen.
Expression of ykiScer\UAS.cHa does not suppress the poor recovery rate of distal wing clones expressing vgScer\UAS.cKa under the control of Scer\GAL4tub.PU in a l(2)gl4 mutant background. Extensive cell death is still seen. Survival of proximal clones is somewhat improved, but unlike in l(2)gl4 mutant clones alone, neoplastic transformation is still not observed until day four of clone induction and the clones display dense cell packing and compact nuclear morphology.
Cell clones expressing vgScer\UAS.cKa under the control of Scer\GAL4αTub84B.PL that are also mutant for fz15 and fz2C1 (generated using the MARCM technique) are present in the wing disc 48 hours after heat-shock, with increased clone size further away from the dorsal-ventral boundary. However, at 72 hours after heat shock these mutant clones disappear as a result of cell death. Nonautonomous effects on growth and patterning are also seen in these mutant wing discs.
Expression of vgScer\UAS.cKa under the control of Scer\GAL4αTub84B.PL in cpa43D mutant clones leads to the extrusion of the clones from all regions of the wing disc, rather than from just the wing blade primordium.
Coexpression of dppScer\UAS.cFa and vgScer\UAS.cKa, both under the control of Scer\GAL4bi-md653, leads to an enhancement of the haltere-to-wing transformation seen when either transgene is expressed alone. Almost all haltere cells are transformed to wing-type cells as seen by the large number of bristles and trichomes. The phenotype is fully penetrant in pharate adults and flies expressing both transgenes are lethal at the pharate adult stage.
The defective wing phenotypes seen when sdScer\UAS.cSa or sdΔ200.Scer\UAS are expressed under control of Scer\GAL4vg.boundary in sdETX4 and sd58d mutants is alleviated when vgScer\UAS.cKa is overexpressed.
The wing pouch phenotype seen in larvae expressing vgScer\UAS.cKa under the control of Scer\GAL4A9 is significantly rescued if the larvae are also carrying wgScer\UAS.cLa. Larvae expressing both NICN.Scer\UAS and vgScer\UAS.cKa under the control of Scer\GAL4T113 show striking overgrowth of the eye discs. Other imaginal discs also show overgrowth. This overgrowth phenotype is also seen if the Scer\GAL4dpp.blk1 driver is used instead of Scer\GAL4T113.
Scer\GAL4dpp.blk1-mediated expression in apUGO35 rescues loss of wing phenotype. Mediated expression in SerCS94/SerRX106 flies substantially rescues the wing blade phenotype. Mediated expression in wgl-16/wgl-17 flies restores the wing. Scer\GAL4dpp.blk1-mediated or Scer\GAL4ptc-559.1-mediated expression substantially rescues the wing blade phenotype of Su(H)2/Su(H)8 flies. Scer\GAL4dpp.blk1-mediated co-expression of vgScer\UAS.cKa and SerScer\UAS.cSa leads to significant induction of an ectopic wing margin in vg83b27-R mutants.
The overgrowth of wing tissue in the eye seen in flies expressing vgScer\UAS.cKa under the control of Scer\GAL4dpp.blk1 is partially suppressed if the flies are heterozygous for sd58d and completely suppressed if the flies are hemizygous for sd58d. The phenotype is also partially suppressed if the flies are also coexpressing sdScer\UAS.cSa. Partially suppresses the loss of wing tissue phenotype seen in flies expressing sdScer\UAS.cSa under the control of Scer\GAL4dpp.blk1 at 29oC.