FlyBase Allele Report: Dmel\sd[58d]

General Information

Symbol

Dmel\sd^58d

Species

D. melanogaster

Name

FlyBase ID

FBal0015364

Feature type

allele

Associated gene

Dmel\sd

Associated Insertion(s)

Carried in Construct

Also Known As

sd⁵⁸

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

gamma ray

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Williams et al., 1993)

Mutagen

gamma ray

(Campbell et al., 1991)

Progenitor genotype

Caused by aberration

In(1)sd^58d

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(Srivastava and Bell, 2003)

visible

(Srivastava et al., 2004)

visible, with Scer\GAL4^vg.int2.1, sd^UAS.cVa

(Legent et al., 2006)

visible (with sd^ETX4)

(Srivastava et al., 2004)

visible | recessive

(Williams et al., 1993)

Phenotype Manifest In

crossvein, with Scer\GAL4^vg.int2.1, sd^UAS.cVa

(Legent et al., 2006)

haltere

(Srivastava et al., 2002)

macrochaeta & wing

(Campbell et al., 1992)

wing

(Chow et al., 2004, Srivastava et al., 2004, Srivastava and Bell, 2003, Srivastava et al., 2002, Campbell et al., 1991)

wing, with Scer\GAL4^vg.boundary, sd^Δ88-123.UAS

(Chow et al., 2004)

wing, with Scer\GAL4^vg.boundary, sd^Δ88-159.UAS

(Chow et al., 2004)

wing, with Scer\GAL4^vg.boundary, sd^{Δ124-159.UAS}

(Chow et al., 2004)

wing, with Scer\GAL4^vg.boundary, sd^{Δ160-219.UAS}

(Chow et al., 2004)

wing, with Scer\GAL4^vg.boundary, sd^Δ200.UAS

(Chow et al., 2004)

wing, with Scer\GAL4^vg.int2.1, sd^UAS.cVa

(Legent et al., 2006)

wing (with sd^ETX4)

(Srivastava et al., 2004)

wing | heat sensitive, with Scer\GAL4^vg.boundary, sd^UAS.cSa

(Chow et al., 2004)

wing | heat sensitive, with Scer\GAL4^vg.boundary, sd^Δ87.UAS

(Chow et al., 2004)

wing blade, with Scer\GAL4^vg.int2.1, sd^UAS.cVa

(Legent et al., 2006)

wing disc

(Srivastava and Bell, 2003)

wing disc | somatic clone

(Liu et al., 2000)

wing hinge, with Scer\GAL4^vg.int2.1, sd^UAS.cVa

(Legent et al., 2006)

wing vein, with Scer\GAL4^vg.int2.1, sd^UAS.cVa

(Legent et al., 2006)

Detailed Description

Statement

Reference

The severity of wing defects caused by expression of sd^Scer\UAS.cVa under the control of Scer\GAL4^vg.int2.1 is suppressed by sd^58d/+; in the double mutant flies 61% have a "mild" phenotype (wings are significantly reduced, few veins and crossveins are still distinguishable), 34% have a "severe" phenotype (wing blade growth is severely impaired) and 5% have an "extreme" phenotype (thorax flight appendages are completely missing and no hinge structures are present).

(Legent et al., 2006)

Expression of one of sd^{Δ200.Scer\UAS}, sd^{Δ124-159.Scer\UAS}, sd^{Δ88-159.Scer\UAS}, sd^{Δ160-219.Scer\UAS} and sd^{Δ88-123.Scer\UAS} when driven by Scer\GAL4^vg.boundary, lead to an enhancement of the wing phenotype seen in sd^ETX4 mutants. Expression of either sd^{Δ87.Scer\UAS} or sd^Scer\UAS.cSa driven by Scer\GAL4^vg.boundary can either rescue the sd^58d defective wing phenotype, or can actually make this phenotype more severe, depending on the insertion lines used. The effect of these insertions is also temperature sensitive, with certain insertions suppressing the sd^58d phenotype at one temperature and enhancing it at another.

(Chow et al., 2004)

Dorsal medial muscles in sd^58d mutants display no obvious phenotype.

(Bernard et al., 2003)

Mutant wing discs show a lower number of mitotically active cells, as well as a smaller wing pouch. Mutant animals small malformed small wings.

(Srivastava and Bell, 2003)

Mutant animals show extensive loss of wing tissue and diminutive halteres.

(Srivastava et al., 2002)

In developing imaginal discs, sd^58d mutant clones can sometimes be recovered even 4 days after clone induction in proximal regions of the wing blade, but not in distal regions.

(Liu et al., 2000)

Strong wing reduction.

(Williams et al., 1993)

Ectopic bristles on the distal wing blade.

(Campbell et al., 1992)

Incomplete penetrance of the nibbled wing margin phenotype. Ectopic bristles are never seen on the wing veins.

(Campbell et al., 1991)

Wings reduced to vestiges, like vg. Halteres and bristles also like vg. sd^58d/sd has strap-shaped wing. Temperature sensitive; effects of temperature pulses at different developmental stages suggest that wing areas eliminated in a specific order (Simpson, Lawrence and Maschat, 1981). RK2A.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

sd^58d has abnormal mitotic cell cycle phenotype, suppressible by sd::vg^UAS.sdTEA/Scer\GAL4^vg.boundary

(Srivastava and Bell, 2003)

Suppressor of

Statement

Reference

sd^58d is a suppressor of visible phenotype of Scer\GAL4^dpp.blk1, vg^UAS.cKa

(Simmonds et al., 1998)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^vg.boundary, sd^Δ200.UAS, sd^58d has wing phenotype, suppressible by vg^UAS.cKa/Scer\GAL4^vg.boundary

(Chow et al., 2004)

Scer\GAL4^vg.boundary, sd^58d, sd^UAS.cSa has wing phenotype, suppressible by vg^UAS.cKa/Scer\GAL4^vg.boundary

(Chow et al., 2004)

sd^58d has wing phenotype, suppressible by wg^UAS.lacZ/Scer\GAL4^vg.boundary

(Srivastava and Bell, 2003)

sd^58d has wing disc phenotype, suppressible by sd::vg^UAS.sdTEA/Scer\GAL4^vg.boundary

(Srivastava and Bell, 2003)

sd^58d has haltere phenotype, suppressible by sd::vg^UAS.sdTEA/Scer\GAL4^vg.PM

(Srivastava et al., 2002)

sd^58d has wing phenotype, suppressible by sd::vg^UAS.sdTEA/Scer\GAL4^vg.PM

(Srivastava et al., 2002)

Suppressor of

Statement

Reference

sd^58d is a suppressor of macrochaeta | ectopic phenotype of Scer\GAL4^dpp.blk1, sens^UAS.cNa

(Srivastava and Bell, 2003)

sd^58d is a suppressor of wing | ectopic phenotype of Scer\GAL4^dpp.blk1, vg^UAS.cKa

(Simmonds et al., 1998)

sd^58d is a suppressor of eye phenotype of Scer\GAL4^dpp.blk1, vg^UAS.cKa

(Simmonds et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

The defective wing phenotypes seen when sd^Scer\UAS.cSa or sd^{Δ200.Scer\UAS} are expressed under control of Scer\GAL4^vg.boundary in sd^58d mutants is alleviated when vg^Scer\UAS.cKa is overexpressed.

(Chow et al., 2004)

sd^58d/+, vg^null/+ females display a wildtype wing phenotype.

(Delanoue et al., 2004)

In sens^Scer\UAS.cNa, Scer\GAL4^dpp.blk1, sd^58d animals no ectopic bristles are seen on the wing.

(Srivastava and Bell, 2003)

The addition of sd::vg^{Scer\UAS.sdTEA} (driven by Scer\GAL4^vg.PM) to sd^58d animals, almost completely rescues the wing and haltere phenotype. The wing margin bristles are almost completely restored and the size of the haltere is also restored.

(Srivastava et al., 2002)

Flies expressing vg^Scer\UAS.cKa under the control of Scer\GAL4^dpp.blk1 show massive outgrowth of wing tissue from the eye. This phenotype is partially suppressed if the flies are heterozygous for sd^58d and completely suppressed if the flies are hemizygous for sd^58d.

(Simmonds et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

sd^58d is rescued by sd^UAS.cSa/Scer\GAL4^vg.boundary

(Magico and Bell, 2011)

Not rescued by

sd^58d is not rescued by sd^{Δ282-344.UAS}/Scer\GAL4^vg.boundary

(Chow et al., 2004)

sd^58d is not rescued by sd^{Δ220-281.UAS}/Scer\GAL4^vg.boundary

(Chow et al., 2004)

sd^58d is not rescued by sd^{Δ220-344.UAS}/Scer\GAL4^vg.boundary

(Chow et al., 2004)

sd^58d is not rescued by sd^Δ96.UAS/Scer\GAL4^vg.boundary

(Chow et al., 2004)

Comments

Expression of sd^Scer\UAS.cSa under the control of Scer\GAL4^vg.boundary restores wing development in sd^58d mutants.

Expression of sd^{Scer\UAS.T:Zzzz\FLAG,T:Uuuu\MyrPalm2} under the control of Scer\GAL4^vg.boundary fails to restore wing development in sd^58d mutants.

Expression of sd^{mNLS.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^vg.boundary fails to restore wing development in sd^58d mutants.

(Magico and Bell, 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9370

In(1)sd^58d, w^* sd^58d P{neoFRT}18A/FM7a

Notes on Origin

Discoverer

Ives, 14th April 1958.

Comments

Cis acting control mutant.

(Williams et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

sd⁵⁸

(Legent et al., 2006, Cho and Irvine, 2004, Delanoue et al., 2004, Bernard et al., 2003, Lunde et al., 2003, Srivastava and Bell, 2003, Delanoue et al., 2002, Srivastava et al., 2002, Guss et al., 2001, Liu et al., 2000, Vaudin et al., 1999, Simmonds et al., 1998, Williams et al., 1993)

sd^58d

(Magico and Bell, 2011)

Name Synonyms

Secondary FlyBase IDs

References (20)

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