FB2026_02 , released June 18, 2026
Allele: Dmel\His3.3AK27M.UASp
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General Information
Symbol
Dmel\His3.3AK27M.UASp
Species
D. melanogaster
Name
FlyBase ID
FBal0359443
Feature type
allele
Associated gene
Dmel\His3.3A
Associated Insertion(s)
Carried in Construct
P{UASp-His3.3A.K27M}
Key Links
Transgenic product class
missense_variant
(Ahmad and Spens, 2019)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Ahmad and Spens, 2019)
Mutagen
in vitro construct
(Ahmad and Spens, 2019)
Progenitor genotype
Carried in construct
P{UASp-His3.3A.K27M}
Cytology
Description

UASp regulatory sequences drive expression of His3.3A that carries a K27M amino acid replacement.

(Ahmad and Spens, 2019)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
His3.3A
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      model of  diffuse midline glioma, H3 K27M-mutant
      is exacerbated by vgUAS.cKa
      (Ahmad and Henikoff, 2021)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      This allele represents a human variant implicated in disease.
      (Ahmad and Henikoff, 2021)
      H3-3A:p.Lys28Met
      (FlyBase curators, 2019-)
      Variants Synonym(s)
      H3-3A:p.Lys27Met
      Associated human disease model(s)
      External database links
      ClinVar: H3-3A_376435
      Comments concerning this variant

      Alternate designation K27M: the histone 3 polypeptide undergoes co-translational N-terminal methionine cleavage, thus the functional protein is one amino acid shorter.

      (FlyBase curators, 2019-)
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      His3.3AK27M.UASp
      Name Synonyms
      Secondary FlyBase IDs
        References (3)