FlyBase Allele Report: Dmel\toy[UAS.cCa]

General Information

Symbol

Dmel\toy^UAS.cCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Czerny

FlyBase ID

FBal0096657

Feature type

allele

Associated gene

Dmel\toy

Associated Insertion(s)

Carried in Construct

P{UAS-toy.C}

Also Known As

UAS-toy

Key Links

Transgenic product class

cDNA

(Czerny et al., 1999)

wild_type

(Czerny et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Czerny et al., 1999)

wild_type

(Czerny et al., 1999)

Mutagen

in vitro construct

(Czerny et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-toy.C}

Cytology

Description

Expression of a 1.7kb toy cDNA is governed by UASt regulatory sequences.

(Czerny et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

toy

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^ey-OK107, toy^hdl

(Furukubo-Tokunaga et al., 2009)

lethal - all die before end of larval stage, with Scer\GAL4^ey-OK107

(Furukubo-Tokunaga et al., 2009)

some die during larval stage, with Scer\GAL4^ey-OK107

(Furukubo-Tokunaga et al., 2009)

visible, with Scer\GAL4^dpp.blk1

(Czerny et al., 1999)

Phenotype Manifest In

adult mushroom body, with Scer\GAL4^ey-OK107

(Furukubo-Tokunaga et al., 2009)

antenna, with Scer\GAL4^dpp.blk1

(Punzo et al., 2004)

eye | ectopic, with Scer\GAL4^dpp.blk1

(Czerny et al., 1999)

eye | ectopic | heat sensitive, with Scer\GAL4^dpp.blk1

(Punzo et al., 2004)

larval brain, with Scer\GAL4^ey-OK107, toy^hdl

(Furukubo-Tokunaga et al., 2009)

leg, with Scer\GAL4^dpp.blk1

(Punzo et al., 2004, Czerny et al., 1999)

leg & eye | ectopic, with Scer\GAL4^dpp.blk1

(Czerny et al., 1999)

symmetrical commissure, with Scer\GAL4^ey-OK107, toy^hdl

(Furukubo-Tokunaga et al., 2009)

vertical lobe of adult mushroom body, with Scer\GAL4^ey-OK107

(Furukubo-Tokunaga et al., 2009)

wing, with Scer\GAL4^dpp.blk1

(Czerny et al., 1999)

wing & eye | ectopic, with Scer\GAL4^dpp.blk1

(Czerny et al., 1999)

Detailed Description

Statement

Reference

Expression of toy^Scer\UAS.cCa driven by Scer\GAL4^so.7 brings about no significant effect on ocellus development.

(Blanco et al., 2010)

Overexpression of toy^UAS.cCa under the control of Scer\GAL4^ey-OK107 results in larval lethality. The larval mushroom bodies of these animals exhibit marked abnormalities, with half exhibiting either degeneration or absence of the dorsal lobes.

(Furukubo-Tokunaga et al., 2009)

Expression of toy^Scer\UAS.cCa via Scer\GAL4^cb41, Scer\GAL4^cb49 or Scer\GAL4^sd.PU fails to induce ectopic eyes.

(Weasner et al., 2007)

toy^Scer\UAS.cCa; Scer\GAL4^dpp.blk1 flies have ectopic eyes on various appendages if raised at 25'C. However, if raised at 18'C appendage truncation is seen, but not ectopic eyes.

(Punzo et al., 2004)

Expression of toy^Scer\UAS.cCa when driven by Scer\GAL4^sca-537.4, Scer\GAL4¹⁴⁰⁷ or Scer\GAL4^elav-C155 does not lead to any noticeable embryonic CNS defects.

(Kammermeier et al., 2001)

When expression is driven by Scer\GAL4^dpp.blk1 ectopic eyes form on the legs and wing.

(Czerny et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

toy^UAS.cCa/Scer\GAL4^toy2 is a suppressor of lethal | pupal stage phenotype of ey^D

(Jacobsson et al., 2009)

toy^UAS.cCa/Scer\GAL4^toy1 is a suppressor of lethal | pupal stage phenotype of ey^J5.71

(Jacobsson et al., 2009)

Other

Statement

Reference

Scer\GAL4^dpp.blk1, so¹, toy^UAS.cCa has visible phenotype

(Niimi et al., 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^dpp.blk1, toy^UAS.cCa has eye | ectopic phenotype, suppressible by ey²

(Czerny et al., 1999)

NOT suppressed by

Statement

Reference

toy^UAS.cCa has eye | ectopic phenotype, non-suppressible by Scer\GAL4^dpp.blk1, ey²/ey²

(Punzo et al., 2004)

toy^UAS.cCa has eye | ectopic phenotype, non-suppressible by Scer\GAL4^dpp.blk1, ey^J5.71/ey^J5.71

(Punzo et al., 2004)

Suppressor of

Statement

Reference

toy^UAS.cCa/Scer\GAL4^toy1 is a suppressor of eye phenotype of ey^J5.71

(Jacobsson et al., 2009)

toy^UAS.cCa/Scer\GAL4^toy2 is a suppressor of adult head phenotype of ey^D

(Jacobsson et al., 2009)

toy^UAS.cCa/Scer\GAL4^toy2 is a suppressor of eye-antennal disc phenotype of ey^D

(Jacobsson et al., 2009)

toy^UAS.cCa/Scer\GAL4^toy2 is a suppressor of eye phenotype of ey^D

(Jacobsson et al., 2009)

toy^UAS.cCa/Scer\GAL4^ey.PH is a suppressor of eye phenotype of ey^J5.71

(Punzo et al., 2002)

NOT Suppressor of

Statement

Reference

toy^UAS.cCa/Scer\GAL4^ey.PH is a non-suppressor of eye phenotype of Scer\GAL4^ey.PH, fng^UAS.cKa

(Dominguez et al., 2004)

Other

Statement

Reference

N^ECN.UAS.cGa, Scer\GAL4^dpp.blk1, toy^UAS.cCa has eye | ectopic phenotype

(Kurata et al., 2000)

Scer\GAL4^dpp.blk1, so¹, toy^UAS.cCa has eye | ectopic phenotype

(Niimi et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Simultaneous overexpression of toy^Scer\UAS.cCa and oc^Scer\UAS.cBa under the control of Scer\GAL4^so.7 gives rise to adult flies with bigger ocelli and, occasionally, ectopic ocelli.

(Blanco et al., 2010)

Expression of toy^Scer\UAS.cCa under the control of Scer\GAL4^toy1 significantly suppresses the lethality seen in ey^D mutants, raising the fraction of homozygous adults from 0.27% to 7.3%. The morphology of compound eyes in these flies is also improved by targeted expression of toy^Scer\UAS.cCa, but never to wild-type proportions. A small eye phenotype is found in 76% of rescued flies.

Expression of toy^Scer\UAS.cCa under the control of Scer\GAL4^toy1 significantly suppresses the lethality seen in ey^J5.71 mutants, raising the fraction of homozygous adults from 12% to 26.8% at 18[o]C. The compound eyes of ey^J5.71 homozygotes are fully restored by one copy of toy^Scer\UAS.cCa.

(Jacobsson et al., 2009)

Scer\GAL4^ey.PH-mediated expression of toy^Scer\UAS.cCa has no effect on the small eye phenotype seen when fng^Scer\UAS.cKa is expressed under the control of Scer\GAL4^ey.PH.

(Dominguez et al., 2004)

The ectopic eyes seen in toy^Scer\UAS.cCa; Scer\GAL4^dpp.blk1 flies are not suppressed by ey²/ey² or ey^J5.71/ey^J5.71.

(Punzo et al., 2004)

Flies expressing N^{ECN.Scer\UAS.cGa} and toy^Scer\UAS.cCa driven by Scer\GAL4^dpp.blk1 exhibit ectopic eyes on legs and wings in all flies.

(Kurata et al., 2000)

The ectopic eye phenotype caused when toy^Scer\UAS.cCa expression is driven by Scer\GAL4^dpp.blk1 is suppressed in an ey² mutant background, though the leg is truncated and deformed to a similar extent as when ectopic eyes do form.

(Czerny et al., 1999)

Expression of toy^Scer\UAS.cCa using Scer\GAL4^dpp.blk1 in a so¹/so¹ background leads to the formation of ectopic eyes.

(Niimi et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

toy^UAS.cCa/Scer\GAL4^toy1 rescues toy^G7.39

(Jacobsson et al., 2009)

toy^UAS.cCa/Scer\GAL4^toy2 rescues toy^G7.39

(Jacobsson et al., 2009)

Partially rescues

toy^UAS.cCa/Scer\GAL4^toy.EEP partially rescues toy^hdl

(Blanco et al., 2010)

toy^UAS.cCa/Scer\GAL4^toy1 partially rescues toy^hdl

(Jacobsson et al., 2009)

toy^UAS.cCa/Scer\GAL4^toy2 partially rescues toy^hdl

(Jacobsson et al., 2009)

Comments

Expression of toy^Scer\UAS.cCa under the control of Scer\GAL4^toy.EEP fully rescues the pupal lethality phenotype and partially rescues the embryonic eye developmental defect associated with toy^hdl. The rescued adult flies display variable ocelliless phenotypes.

(Blanco et al., 2010)

Expression of toy^Scer\UAS.cCa under the control of Scer\GAL4^toy1 or Scer\GAL4^toy2 does not rescue toy^hdl homozygous mutants raised at 29[o]C. However, at lower temperatures (such as 15-25[o]C) rescue is significant. Unexpectedly, equally high rescue is seen when only the toy^Scer\UAS.cCa or Scer\GAL4^toy1/Scer\GAL4^toy2 transgenes alone are present.

Expression of toy^Scer\UAS.cCa under the control of Scer\GAL4^toy1 or Scer\GAL4^toy2 rescues the ocellar phenotype found in toy^hdl mutants in 50% of flies.

The presence of toy^Scer\UAS.cCa, without a Scer\GAL4 driver can rescue toy^hdl at high temperatures (e.g. >25[o]C).

Expression of toy^Scer\UAS.cCa under the control of Scer\GAL4^toy1 or Scer\GAL4^toy2 is able to rescue homozygous mutant toy^G7.39 flies.

Expression of toy^Scer\UAS.cCa under the control of Scer\GAL4^toy1 or Scer\GAL4^toy2 rescues the ocellar phenotype found in toy^G7.39 mutants in 90% of flies.

The presence of toy^Scer\UAS.cCa, without a Scer\GAL4 driver can rescue toy^G7.39 at high temperatures (e.g. >25[o]C).

(Jacobsson et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

26805

y¹ w^*; P{UAS-toy.C}8

26799

y¹ w^*; P{UAS-toy.C}11

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

toy^Scer\UAS.cCa

toy^UAS.cCa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Czerny

(Czerny et al., 1999)

Secondary FlyBase IDs

References (19)

Report Sections

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