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General Information
Symbol
Dmel\NECN.UAS.cGa
Species
D. melanogaster
Name
FlyBase ID
FBal0090564
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-NDN, UAS-NEXT, UAS-NotchDN, Necd, UAS-dnNotch
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a dominant negative form of N in which most of the intracellular domain is deleted.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Third instar larval eye-antennal discs expressing NECN.Scer\UAS.cGa under the control of Scer\GAL4hth-GETDB show a disruption of epithelial folding at the A1 fold, and abnormal mixing of cells on either side of the A1 field boundary, as compared to controls.

Third instar larval eye-antennal disc clones expressing NECN.Scer\UAS.cGa under the control of Scer\GAL4Act5C.PI, and not spanning the A1 fold, do not show abnormal mixing of cells on either side of the A1 field boundary, but cells show a larger apical area than those normally found in the fold.

Larval eye-antennal discs expressing NECN.Scer\UAS.cGa under the control of Scer\GAL4dpp.blk1 exhibit decreased cell proliferation, but no significant change in apoptosis levels, as compared to controls.

Expression of NECN.Scer\UAS.cGa under the control of Scer\GAL4E(spl)m6-BFM.PF has no significant effect on the number of abdominal lateral adult muscle precursor cells in mid-third instar larvae, as compared to controls.

Expression of NECN.Scer\UAS.cGa in the eye disc under the control of Scer\GAL4Act5C.PP results in clones that overproliferate compared to wild type cells.

Expression of NECN.Scer\UAS.cGa under the control of Scer\GAL4ey.PH results in a dramatic loss of the eye.

NECN.Scer\UAS.cGa; Scer\GAL41151 pupae have normal numbers of dorso-ventral and medio-lateral indirect flight muscle founder myoblasts and the resulting adults have the correct number of dorso-ventral and medio-lateral indirect flight muscle fibres.

Lymph glands that express (driven by Scer\GAL4Scer\FRT.Act5C) are smaller than wild-type. This phenotype is not due to increased apoptosis.

When NECN.Scer\UAS.cGa expressing clones (driven by Scer\GAL4Act5C.PP) are made in the ventral part of the wing disc, they are not significantly rounder than wild-type clone.

Neuronal patterning in the ommatidial preclusters is maintained in flies expressing NECN.Scer\UAS.cGa under the control of Scer\GAL4lz-gal4.

Expression of NECN.Scer\UAS.cGa driven by Scer\GAL4ey.PH leads to a severely reduced eye.

Over expression of the dominant negative form of N, NECN.Scer\UAS.cGa in the posterior compartment of the wing disc by Scer\GAL4en-e16E, produces a ragged dorsal/ventral boundary.

Flies expressing NECN.Scer\UAS.cGa under the control of Scer\GAL4vg.int2.1 have wing defects ranging from wing margin notches to rudimentary wings.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of bansponge.Scer\UAS.T:Disc\RFP suppresses the disrupted A1 epithelial folding and cell mixing at the A1 field boundary seen in eye-antennal discs expressing NECN.Scer\UAS.cGa under the control of Scer\GAL4hth-GETDB.

Expression of NECN.Scer\UAS.cGa does not suppress the dendrite pruning defects seen in ddaC neurons expressing lin19GD9650 under the control of Scer\GAL4ppk.PG at 16 hours after pupariation.

Expression of NECN.Scer\UAS.cGa under the control of Scer\GAL4Act5C.PU partially suppresses the oocyte polarity defect seen when the posterior follicle cells are mutant for flwFP41.

Eye disc clones expressing NECN.Scer\UAS.cGa under the control of Scer\GAL4Act5C.PP in a Vha55j2E9 mutant background do not proliferate as in a wild-type background and instead create only small clones, of a similar size to Vha55j2E9 mutant clones.

The eye loss phenotype caused by expression of NECN.Scer\UAS.cGa under the control of Scer\GAL4ey.PH is partially suppressed by co-expression of hipkScer\UAS.T:Ivir\HA1.

Eye development in NECN.Scer\UAS.cGa; Scer\GAL4ey.PH flies is partially rescued by eygScer\UAS.lune. The resulting eyes are almost as big as wild-type.

Flies expressing NECN.Scer\UAS.cGa and either toyScer\UAS.cCa or eyScer\UAS.cHa driven by Scer\GAL4dpp.blk1 exhibit ectopic eyes on legs and wings in all flies.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
NECN.Scer\UAS.cGa
NECN.UAS.cGa
Name Synonyms
Secondary FlyBase IDs
    References (23)