Third instar larval eye-antennal discs expressing NECN.Scer\UAS.cGa under the control of Scer\GAL4hth-GETDB show a disruption of epithelial folding at the A1 fold, and abnormal mixing of cells on either side of the A1 field boundary, as compared to controls.
Third instar larval eye-antennal disc clones expressing NECN.Scer\UAS.cGa under the control of Scer\GAL4Act5C.PI, and not spanning the A1 fold, do not show abnormal mixing of cells on either side of the A1 field boundary, but cells show a larger apical area than those normally found in the fold.
Larval eye-antennal discs expressing NECN.Scer\UAS.cGa under the control of Scer\GAL4dpp.blk1 exhibit decreased cell proliferation, but no significant change in apoptosis levels, as compared to controls.
Expression of NECN.Scer\UAS.cGa under the control of Scer\GAL4E(spl)m6-BFM.PF has no significant effect on the number of abdominal lateral adult muscle precursor cells in mid-third instar larvae, as compared to controls.
NECN.Scer\UAS.cGa; Scer\GAL41151 pupae have normal numbers of dorso-ventral and medio-lateral indirect flight muscle founder myoblasts and the resulting adults have the correct number of dorso-ventral and medio-lateral indirect flight muscle fibres.
Lymph glands that express (driven by Scer\GAL4Scer\FRT.Act5C) are smaller than wild-type. This phenotype is not due to increased apoptosis.
When NECN.Scer\UAS.cGa expressing clones (driven by Scer\GAL4Act5C.PP) are made in the ventral part of the wing disc, they are not significantly rounder than wild-type clone.
Over expression of the dominant negative form of N, NECN.Scer\UAS.cGa in the posterior compartment of the wing disc by Scer\GAL4en-e16E, produces a ragged dorsal/ventral boundary.