FlyBase Allele Report: Dmel\Psn[UAS.cYa]

General Information

Symbol

Dmel\Psn^UAS.cYa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Ye

FlyBase ID

FBal0094649

Feature type

allele

Associated gene

Dmel\Psn

Associated Insertion(s)

Carried in Construct

P{UAS-PS.Y}

Key Links

Transgenic product class

wild_type

(Ye and Fortini, 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ye and Fortini, 1998)

Mutagen

in vitro construct

(Hu and Fortini, 2003, Ye and Fortini, 1998)

Progenitor genotype

Carried in construct

P{UAS-PS.Y}

Cytology

Description

UAS regulatory sequences drive Psn expression.

(Ye and Fortini, 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Psn

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult head bristle | ectopic, with Scer\GAL4^A9

(Wangler et al., 2011)

campaniform sensillum L3-1 of wing vein L3 | ectopic, with Scer\GAL4^71B

(Wangler et al., 2011)

campaniform sensillum L3-2 of wing vein L3 | ectopic, with Scer\GAL4^71B

(Wangler et al., 2011)

campaniform sensillum L3-3 of wing vein L3 | ectopic, with Scer\GAL4^71B

(Wangler et al., 2011)

scutellar bristle | ectopic, with Scer\GAL4^Bx-MS1096

(Wangler et al., 2011)

sternopleural bristle | ectopic, with Scer\GAL4^71B

(Wangler et al., 2011)

wing, with Scer\GAL4^71B

(Wangler et al., 2011)

wing, with Scer\GAL4^Bx-MS1096

(Wangler et al., 2011)

wing margin bristle | increased number, with Scer\GAL4^71B

(Wangler et al., 2011)

wing vein L3, with Scer\GAL4^71B

(Wangler et al., 2011)

wing vein L3, with Scer\GAL4^Bx-MS1096

(Wangler et al., 2011)

wing vein L5, with Scer\GAL4^71B

(Wangler et al., 2011)

wing vein L5, with Scer\GAL4^Bx-MS1096

(Wangler et al., 2011)

Detailed Description

Statement

Reference

Expression of Psn^Scer\UAS.cYa in the dorsal region of the wing pouch, with weaker expression ventrally, under the control of Scer\GAL4^Bx-MS1096 leads to thickening of the L3 and L5 wing veins and a significant reduction in the size of the wing surface resulting in dorsal curling of the wings.

Expression of Psn^Scer\UAS.cYa under the control of Scer\GAL4^71B results in a modest number of ectopic sensory organs in a fraction of progeny, such as supernumerary double row bristles along the wing margin at the junction with the L3 wing vein and extra campaniform sensilla along the L3 vein.

Expression of Psn^Scer\UAS.cYa under the control of Scer\GAL4^A9 results in a few ectopic bristles anterior to the eye.

Psn^Scer\UAS.cYa expression under the control of Scer\GAL4^71B produces a number of thick ectopic sternopleural bristles.

Psn^Scer\UAS.cYa expression under the control of Scer\GAL4^Bx-MS1096 produces a number of ectopic scutellar macrochaetae.

(Wangler et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Psn^UAS.cYa, Scer\GAL4^Bx-MS1096 has wing vein L3 phenotype, enhanceable by Hsap\PRDX1^UAS.cWa, Scer\GAL4^Bx-MS1096

(Wangler et al., 2011)

Psn^UAS.cYa, Scer\GAL4^Bx-MS1096 has wing vein L5 phenotype, enhanceable by Hsap\PRDX1^UAS.cWa, Scer\GAL4^Bx-MS1096

(Wangler et al., 2011)

Psn^UAS.cYa, Scer\GAL4^A9 has adult head bristle | ectopic phenotype, enhanceable by Hsap\PRDX2^UAS.cWa/Scer\GAL4^Bx-MS1096

(Wangler et al., 2011)

Psn^UAS.cYa, Scer\GAL4^A9 has adult head bristle | ectopic phenotype, enhanceable by Scer\GAL4^Bx-MS1096/Hsap\PRDX1^UAS.cWa

(Wangler et al., 2011)

Psn^UAS.cYa, Scer\GAL4^A9 has adult head bristle | ectopic phenotype, enhanceable by Hsap\PRDX2^UAS.cWa/Scer\GAL4^Bx-MS1096/Hsap\PRDX1^UAS.cWa

(Wangler et al., 2011)

Psn^UAS.cYa, Scer\GAL4^Bx-MS1096 has wing phenotype, enhanceable by Hsap\PRDX1^UAS.cWa, Scer\GAL4^Bx-MS1096

(Wangler et al., 2011)

Psn^UAS.cYa, Scer\GAL4^Bx-MS1096 has wing vein L3 phenotype, enhanceable by Hsap\PRDX2^UAS.cWa, Scer\GAL4^Bx-MS1096

(Wangler et al., 2011)

Psn^UAS.cYa, Scer\GAL4^Bx-MS1096 has wing vein L5 phenotype, enhanceable by Hsap\PRDX2^UAS.cWa, Scer\GAL4^Bx-MS1096

(Wangler et al., 2011)

Psn^UAS.cYa, Scer\GAL4^Bx-MS1096 has wing phenotype, enhanceable by Hsap\PRDX2^UAS.cWa, Scer\GAL4^Bx-MS1096

(Wangler et al., 2011)

Psn^UAS.cYa, Scer\GAL4^Bx-MS1096 has wing vein L3 phenotype, enhanceable by Hsap\PRDX2^UAS.cWa/Hsap\PRDX1^UAS.cWa, Scer\GAL4^Bx-MS1096

(Wangler et al., 2011)

Psn^UAS.cYa, Scer\GAL4^Bx-MS1096 has wing vein L5 phenotype, enhanceable by Hsap\PRDX2^UAS.cWa/Hsap\PRDX1^UAS.cWa, Scer\GAL4^Bx-MS1096

(Wangler et al., 2011)

Psn^UAS.cYa, Scer\GAL4^Bx-MS1096 has wing phenotype, enhanceable by Hsap\PRDX2^UAS.cWa/Hsap\PRDX1^UAS.cWa, Scer\GAL4^Bx-MS1096

(Wangler et al., 2011)

Psn^UAS.cYa, Scer\GAL4^Bx-MS1096 has scutellar bristle | ectopic phenotype, enhanceable by Hsap\PRDX2^UAS.cWa/Hsap\PRDX1^UAS.cWa, Scer\GAL4^Bx-MS1096

(Wangler et al., 2011)

Suppressed by

Statement

Reference

Hsap\PRDX1^UAS.cWa, Hsap\PRDX2^UAS.cWa, Psn^UAS.cYa, Scer\GAL4^Bx-MS1096 has wing vein L3 phenotype, suppressible by N^Ax-M1

(Wangler et al., 2011)

Hsap\PRDX1^UAS.cWa, Hsap\PRDX2^UAS.cWa, Psn^UAS.cYa, Scer\GAL4^Bx-MS1096 has wing vein L5 phenotype, suppressible by N^Ax-M1

(Wangler et al., 2011)

Hsap\PRDX1^UAS.cWa, Hsap\PRDX2^UAS.cWa, Psn^UAS.cYa, Scer\GAL4^Bx-MS1096 has wing phenotype, suppressible by N^Ax-M1

(Wangler et al., 2011)

Hsap\PRDX1^UAS.cWa, Hsap\PRDX2^UAS.cWa, Psn^UAS.cYa, Scer\GAL4^Bx-MS1096 has scutellar bristle | ectopic phenotype, suppressible by N^Ax-M1

(Wangler et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of either Hsap\PRDX1^Scer\UAS.cWa, Hsap\PRDX2^Scer\UAS.cWa or both, in the developing wing under the control of Scer\GAL4^Bx-MS1096, increases the severity of the vein thickening phenotypes seen upon expression of Psn^Scer\UAS.cYa. The wings are also reduced in size and exhibit stronger dorsal curling.

Co-expression of either Hsap\PRDX1^Scer\UAS.cWa, Hsap\PRDX2^Scer\UAS.cWa or both with Psn^Scer\UAS.cYa, under the control of Scer\GAL4^71B greatly increases the severity of ectopic clusters of both double-row bristles and campaniform sensilla and markedly enhances the number of thick ectopic sternopleural bristles.

Co-expression of both Hsap\PRDX1^Scer\UAS.cWa, Hsap\PRDX2^Scer\UAS.cWa with Psn^Scer\UAS.cYa, under the control of Scer\GAL4^A9 increases the number of ectopic bristles anterior to the eye, compared to expression of Psn^Scer\UAS.cYa alone.

Co-expression of both Hsap\PRDX1^Scer\UAS.cWa, Hsap\PRDX2^Scer\UAS.cWa enhances the ectopic scutellar macrochaetae phenotype found upon expression of Psn^Scer\UAS.cYa under the control of Scer\GAL4^Bx-MS1096.

Co-expression of Psn^Scer\UAS.cYa, Hsap\PRDX1^Scer\UAS.cWa, and Hsap\PRDX2^Scer\UAS.cWa with Scer\GAL4^Bx-MS1096 in flies carrying N^Ax-M1 only results in residual thickening of the L3 and L5 wing veins, which is significantly less severe than that observed in response to expression of Psn^Scer\UAS.cYa, Hsap\PRDX1^Scer\UAS.cWa, and Hsap\PRDX2^Scer\UAS.cWa in a wild-type background.

The ectopic scutellar macrochaetae of flies co-expressing Psn^Scer\UAS.cYa, Hsap\PRDX1^Scer\UAS.cWa, and Hsap\PRDX2^Scer\UAS.cWa under the control of Scer\GAL4^Bx-MS1096 is nearly completely suppressed by N^Ax-M1.

(Wangler et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in the plasmid "pIZPsnV5His" and transfected into S2 cells to assess γ-secretase assembly and activity.

(Hu and Fortini, 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

PS^Scer\UAS.cYa

PS^UAS.cYa

Psn^Scer\UAS.cYa

Psn^UAS.cYa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Ye

(Ye and Fortini, 1998)

Secondary FlyBase IDs

References (3)

Report Sections

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