PS, Dps, dPsn, γ-secretase, dPresenilin
transmembrane protein involved in proteolysis of Notch - also processes βAPP - Presenilin and Nicastrin are essential components of γ-secretase, a multi-subunit protease that cleaves Type I transmembrane proteins
Gene model reviewed during 5.41
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.46
Homodimer (By similarity). Component of the gamma-secretase complex, a complex composed of a presenilin (Psn) homodimer, nicastrin (Nct), Aph-1 and Pen-2. Interacts with Metl. Isoform 2 shows a better interaction with Metl than isoform 1.
Cleaved. The cleavage, which probably takes place between the 6th and the 7th transmembrane regions, may be required for activation of the gamma-secretase activity.
The PAL motif is required for normal active site conformation.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Psn using the Feature Mapper tool.
RT-PCR analysis of Psn transcript supports the expression of three isoforms at all developmental stages.
The Psn transcript is detected in nurse cells and the oocyte during oogenesis. In larvae the Psn transcript is detected in eye-antennal discs in regions that give rise to the occellar and interoccellar bristles, and in the posterior region of the leg disc which gives rise to the tibia and femur.
PS protein is expressed in most cells in the developing embryo and during oogenesis. Higher levels of expression are detected in the somatic cells during oogenesis, as well as in the ectoderm durning embryogenesis. During larval and pupal stages PS is expressed in most tissues. Expression in the wing disc and the eye disc is detacted associated with apical cell membranes and in the photoreceptors.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Psn in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Loss of Psn function increases levels of apoptosis in developing tissues. Overexpression of Psn causes apoptotic and neurogenic phenotypes resembling those of Psn loss-of-function mutants, suggesting that Psn exerts a dominant negative effect when expressed at high levels. Psn-induced apoptosis is cell autonomous and can be blocked by constitutive N activation.
Symbol Psn adopted, rather than 'PS', to avoid confusion with Position Specific integrin genes.
Isolation and characterisation of the homolog of the presenilin genes.