FlyBase Allele Report: Dmel\Appl[UAS.cCSa]

General Information

Symbol

Dmel\Appl^UAS.cCSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Carmine-Simmen

FlyBase ID

FBal0221088

Feature type

allele

Associated gene

Dmel\Appl

Associated Insertion(s)

Carried in Construct

P{UAS-Appl.CS}

Also Known As

UAS-APPL

Key Links

Transgenic product class

wild_type

(Carmine-Simmen et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Carmine-Simmen et al., 2009)

Mutagen

in vitro construct

(Carmine-Simmen et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-Appl.CS}

Cytology

Description

UASt regulatory sequences drive expression of full-length Appl.

(Carmine-Simmen et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Appl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Alzheimer's disease

CEA

(Carmine-Simmen et al., 2009)

CEA with Psn^UAS.cUa

(Ramaker et al., 2016)

model of Alzheimer's disease 1

CEA

(Rieche et al., 2018, Ramaker et al., 2016, Blake et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Alzheimer's disease

is exacerbated by Bace^UAS.cCSa

(Carmine-Simmen et al., 2009)

is ameliorated by Psn^UAS.cUa

(Ramaker et al., 2016)

model of Alzheimer's disease 1

is ameliorated by Fas2^UAS.cLa

(Rieche et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^GMR.PU

(Bolkan et al., 2012)

abnormal phototaxis | adult stage | progressive, with Scer\GAL4^elav.PU

(Bolkan et al., 2012)

Phenotype Manifest In

adult brain, with Scer\GAL4^Appl.G1a

(Carmine-Simmen et al., 2009)

adult brain, with Scer\GAL4^elav-C155

(Carmine-Simmen et al., 2009)

cell body rind of adult lamina, with Scer\GAL4^GMR.PU

(Bolkan et al., 2012)

retina, with Scer\GAL4^GMR.PF

(Carmine-Simmen et al., 2009)

Detailed Description

Statement

Reference

28 day old flies expressing Appl^{Scer\UAS.cCSa} under the control of Scer\GAL4^GMR.PU show some vacuoles in the lamina cortex.

Flies expressing Appl^{Scer\UAS.cCSa} under the control of Scer\GAL4^elav.PU show a dramatic age-dependent decline in their performance index in a fast phototaxis assay.

(Bolkan et al., 2012)

30 day old flies expressing Appl^{Scer\UAS.cCSa} under the control of Scer\GAL4^GMR.PF have dense aggregates in the retina (these aggregates are not seen in these flies at 1 or 5 days after eclosion or in wild-type controls). These aggregates have characteristics of amyloid deposits.

Expression of Appl^{Scer\UAS.cCSa} under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^Appl.G1a results in the formation of deposits (that stain with toluidine blue) in the brains of aged flies, although the deposits are quite rare.

Vacuoles are seen in the retina of flies expressing Appl^{Scer\UAS.cCSa} under the control of Scer\GAL4^GMR.PF; they are not seen in 1-2 day old flies, but by 10 days after eclosion, an increasing number of small vacuoles scattered throughout the retina are seen, and by 30 days after eclosion, the vacuoles have increased in size and number.

20 day old flies expressing Appl^{Scer\UAS.cCSa} under the control of Scer\GAL4^elav-C155 have a significantly lower performance index in a fast phototaxis assay than young flies or age-matched control flies.

(Carmine-Simmen et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Appl^UAS.cCSa, Scer\GAL4^elav.PU has abnormal phototaxis | adult stage | progressive phenotype, suppressible by Bace⁵²⁴³/Bace[+]

(Bolkan et al., 2012)

NOT Enhancer of

Statement

Reference

Appl^UAS.cCSa, Scer\GAL4^GMR.PU is a non-enhancer of abnormal neuroanatomy | adult stage | progressive phenotype of Bace^GD5366, Scer\GAL4^GMR.PU

(Bolkan et al., 2012)

Suppressor of

Statement

Reference

Scer\GAL4^Appl.G1a/Appl^UAS.cCSa is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of Appl^d, SNF4Aγ^loe

(Wentzell et al., 2012)

Scer\GAL4^elav-C155/Appl^UAS.cCSa is a suppressor of abnormal neuroanatomy | adult stage phenotype of sws¹

(Wentzell et al., 2012)

Scer\GAL4^Appl.G1a/Appl^UAS.cCSa is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of SNF4Aγ^loe

(Wentzell et al., 2012)

NOT Suppressor of

Statement

Reference

Appl^UAS.cCSa, Scer\GAL4^GMR.PU is a non-suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of Bace^GD5366, Scer\GAL4^GMR.PU

(Bolkan et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

Appl^UAS.cCSa, Scer\GAL4^Appl.G1a has adult brain phenotype, enhanceable by Bace^UAS.cCSa, Scer\GAL4^Appl.G1a

(Carmine-Simmen et al., 2009)

Suppressor of

Statement

Reference

Scer\GAL4^Appl.G1a/Appl^UAS.cCSa is a suppressor | partially of larval brain | third instar larval stage phenotype of Appl^d, SNF4Aγ^loe

(Wentzell et al., 2012)

Scer\GAL4^elav-C155/Appl^UAS.cCSa is a suppressor of adult brain phenotype of sws¹

(Wentzell et al., 2012)

Scer\GAL4^Appl.G1a/Appl^UAS.cCSa is a suppressor of larval brain | third instar larval stage phenotype of SNF4Aγ^loe

(Wentzell et al., 2012)

Other

Statement

Reference

Appl^UAS.cCSa, Bace^GD5366, Scer\GAL4^GMR.PU has lamina phenotype

(Bolkan et al., 2012)

Appl^UAS.cCSa, Bace^UAS.cCSa, Scer\GAL4^Appl.G1a has adult brain phenotype

(Carmine-Simmen et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Appl^{Scer\UAS.cCSa} does not increase in general the lamina degeneration phenotype seen in 28 day old flies expressing Bace^GD5366 under the control of Scer\GAL4^GMR.PU, although vacuoles are occasionally seen in the lamina neuropil in these flies.

The age-dependent decline in performance index in a fast phototaxis assay of flies expressing Appl^{Scer\UAS.cCSa} under the control of Scer\GAL4^elav.PU is suppressed if they are also heterozygous for Bace⁵²⁴³. The flies also show an increase in survival.

(Bolkan et al., 2012)

Expression of Appl^{Scer\UAS.cCSa} under the control of Scer\GAL4^Appl.G1a substantially suppresses the increase in vacuolisation seen in the brains of SNF4Aγ^loe mutant third instar larvae.

Expression of Appl^{Scer\UAS.cCSa} under the control of Scer\GAL4^Appl.G1a partially suppresses the vacuolisation seen in 4 day old Appl^d SNF4Aγ^loe double mutant males, returning the phenotype to the level seen in SNF4Aγ^loe single mutants.

Expression of Appl^{Scer\UAS.cCSa} under the control of Scer\GAL4^elav-C155 fails to suppress the vacuolisation seen in the brains of sws¹ mutant 14 day old males.

(Wentzell et al., 2012)

Co-expression of Bace^{Scer\UAS.cCSa} results in a marked increase in the amyloid deposits seen in flies expressing Appl^{Scer\UAS.cCSa} under the control of Scer\GAL4^GMR.PF.

Co-expression of Bace^{Scer\UAS.cCSa} and Appl^{Scer\UAS.cCSa} under the control of Scer\GAL4^Appl.G1a results in the formation of numerous toluidine blue-positive deposits in the brain. These flies also have spongiform lesions scattered throughout the brain.

20 day old flies co-expressing Bace^{Scer\UAS.cCSa} and Appl^{Scer\UAS.cCSa} under the control of Scer\GAL4^Appl.G1a have fibrillar structures and dense bodies in the axons.

(Carmine-Simmen et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Appl^{Scer\UAS.cCSa}

Appl^UAS.cCSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Carmine-Simmen

Secondary FlyBase IDs

References (6)

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