FlyBase Allele Report: Dmel\Bace[UAS.cCSa]

General Information

Symbol

Dmel\Bace^UAS.cCSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Carmine-Simmen

FlyBase ID

FBal0221090

Feature type

allele

Associated gene

Dmel\Bace

Associated Insertion(s)

Carried in Construct

P{UAS-Bace.CS}

Also Known As

UAS-dBACE

Key Links

Transgenic product class

cDNA

(Carmine-Simmen et al., 2009)

wild_type

(Carmine-Simmen et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Carmine-Simmen et al., 2009)

wild_type

(Carmine-Simmen et al., 2009)

Mutagen

in vitro construct

(Carmine-Simmen et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-Bace.CS}

Cytology

Description

UAS regulatory sequences drive expression of Bace cDNA GH11417.

(Carmine-Simmen et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Bace

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Alzheimer's disease

CEA

(Rieche et al., 2018)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alzheimer's disease 1

modeled by Appl^{UAS.mRFP.EGFP}

(Long et al., 2024)

exacerbates Alzheimer's disease

modeled by Appl^d

(Bourdet et al., 2015)

modeled by Appl^UAS.cCSa

(Carmine-Simmen et al., 2009)

ameliorates Alzheimer's disease

modeled by Appl^UAS.cCSa, Psn^UAS.cUa

(Ramaker et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^GMR.PU

(Bolkan et al., 2012)

Phenotype Manifest In

lamina, with Scer\GAL4^GMR.PU

(Bolkan et al., 2012)

Detailed Description

Statement

Reference

Expression of Bace^{Scer\UAS.cCSa} under the control of Scer\GAL4^VT064246 (using tub-Gal80[ts] to limit the expression to adulthood) does not impair either middle- or long-term memory tested in an aversive olfactory memory assay 2hr and 24hr post-training, respectively.

(Turrel et al., 2017)

Adult specific expression of Bace^{Scer\UAS.cCSa} driven by Scer\GAL4^{Mef2.247.Switch} (only activated when adult flies are fed RU486) or Scer\GAL4^c739 (using the TARGET system) does not significantly affect short term memory (2hrs after training) in an olfactory aversive conditioning paradigm (performance index is similar to controls).

(Bourdet et al., 2015)

28 day old flies expressing Bace^{Scer\UAS.cCSa} under the control of Scer\GAL4^GMR.PU show vacuole formation in the lamina and the retina. The vacuoles in the lamina are not restricted to the lamina cortex.

(Bolkan et al., 2012)

No increase in vacuolisation is seen in the brains of 5 days old flies expressing Bace^{Scer\UAS.cCSa} under the control of Scer\GAL4^Appl.G1a.

(Wentzell et al., 2012)

20 day old flies expressing Bace^{Scer\UAS.cCSa} under the control of Scer\GAL4^elav-C155 have a significantly lower performance index in a fast phototaxis assay than young flies or age-matched control flies.

(Carmine-Simmen et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Appl^UAS.cTa, Bace^UAS.cCSa, Scer\GAL4^VT064246 has abnormal memory | adult stage phenotype, suppressible by Nep1^EY21255, Scer\GAL4^VT064246

(Turrel et al., 2017)

Enhancer of

Statement

Reference

Bace^UAS.cCSa, Scer\GAL4^VT064246 is an enhancer of abnormal memory | adult stage phenotype of Nep1^GD11797, Scer\GAL4^VT064246

(Turrel et al., 2017)

Scer\GAL4^{Mef2.247.Switch}/Bace^UAS.cCSa is an enhancer of abnormal memory | adult stage phenotype of Appl^d

(Bourdet et al., 2015)

Scer\GAL4^c739/Bace^UAS.cCSa is an enhancer of abnormal memory | adult stage phenotype of Appl^d

(Bourdet et al., 2015)

Scer\GAL4^Appl.G1a/Bace^UAS.cCSa is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of SNF4Aγ^loe

(Wentzell et al., 2012)

Other

Statement

Reference

Appl^UAS.cTa, Bace^UAS.cCSa, Scer\GAL4^VT064246 has abnormal memory | adult stage phenotype

(Turrel et al., 2017)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^Appl.G1a/Bace^UAS.cCSa is an enhancer of larval brain | third instar larval stage phenotype of SNF4Aγ^loe

(Wentzell et al., 2012)

Bace^UAS.cCSa, Scer\GAL4^Appl.G1a is an enhancer of adult brain phenotype of Appl^UAS.cCSa, Scer\GAL4^Appl.G1a

(Carmine-Simmen et al., 2009)

Other

Statement

Reference

Appl^UAS.cCSa, Bace^UAS.cCSa, Scer\GAL4^Appl.G1a has adult brain phenotype

(Carmine-Simmen et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Flies simultaneously expressing Appl^Scer\UAS.cTa and Bace^{Scer\UAS.cCSa} under the control of Scer\GAL4^VT064246 display deficit in both middle- and long-term memory and this can be rescued by co-expression of Nep1^EY21255.

The impaired middle- or long-term term memory tested in an aversive olfactory memory assay 2hr or 24hr post-training, respectively, characteristic for adult flies expressing Nep1^GD11797 under the control of Scer\GAL4^VT064246 (combined with tub-Gal80[ts] to limit expression to adulthood) are exacerbated further by co-expression of Bace^{Scer\UAS.cCSa}.

(Turrel et al., 2017)

Adult specific expression of Bace^{Scer\UAS.cCSa} driven by Scer\GAL4^{Mef2.247.Switch} (activated in adult flies fed RU486) significantly enhances short term memory defects in an olfactory aversive conditioning paradigm compared to Appl^d/+ flies or to controls not fed RU486; there are no significant effects on learning, olfactory acuity or shock reactivity.

Adult specific expression of Bace^{Scer\UAS.cCSa} driven by Scer\GAL4^c739 (using the TARGET system) significantly enhances short term memory defects in an olfactory aversive conditioning paradigm compared to Appl^d/+ flies or to genetic or temperature controls; there are no significant effects on learning, olfactory acuity or shock reactivity.

(Bourdet et al., 2015)

Expression of Bace^{Scer\UAS.cCSa} under the control of Scer\GAL4^Appl.G1a enhances the increase in vacuolisation seen in the brains of SNF4Aγ^loe mutant third instar larvae.

(Wentzell et al., 2012)

Co-expression of Bace^{Scer\UAS.cCSa} results in a marked increase in the amyloid deposits seen in flies expressing Appl^{Scer\UAS.cCSa} under the control of Scer\GAL4^GMR.PF.

Co-expression of Bace^{Scer\UAS.cCSa} and Appl^{Scer\UAS.cCSa} under the control of Scer\GAL4^Appl.G1a results in the formation of numerous toluidine blue-positive deposits in the brain. These flies also have spongiform lesions scattered throughout the brain.

20 day old flies co-expressing Bace^{Scer\UAS.cCSa} and Appl^{Scer\UAS.cCSa} under the control of Scer\GAL4^Appl.G1a have fibrillar structures and dense bodies in the axons.

(Carmine-Simmen et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^GMR.PU/Bace^UAS.cCSa rescues Bace^GD5366

(Bolkan et al., 2012)

Scer\GAL4^elav.PU/Bace^UAS.cCSa rescues Bace²⁰⁴⁵/Df(2L)Exel7038

(Bolkan et al., 2012)

Scer\GAL4^Bace.5.4/Bace^UAS.cCSa rescues Bace²⁰⁴⁵/Df(2L)Exel7038

(Bolkan et al., 2012)

Partially rescues

Scer\GAL4^αTub84B.PL/Bace^UAS.cCSa partially rescues Bace^GD5366

(Bolkan et al., 2012)

Comments

Co-expression of Bace^{Scer\UAS.cCSa} partially rescues the lethality caused by expression of Bace^GD5366 under the control of Scer\GAL4^αTub84B.PL.

Co-expression of Bace^{Scer\UAS.cCSa} reduces the vacuolisation in the lamina neuropil and cortex of 28 day old flies expressing Bace^GD5366 under the control of Scer\GAL4^GMR.PU to the levels seen in controls.

Expression of Bace^{Scer\UAS.cCSa} under the control of Scer\GAL4^elav.PU significantly reduces the vacuolisation of the lamina neuropil and cortex which is seen in 28 day old Bace²⁰⁴⁵/Df(2L)Exel7038 flies.

Expression of Bace^{Scer\UAS.cCSa} under the control of Scer\GAL4^Bace.5.4 completely rescues the vacuolisation of the lamina neuropil and cortex which is seen in 28 day old Bace²⁰⁴⁵/Df(2L)Exel7038 flies.

(Bolkan et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Bace^{Scer\UAS.cCSa}

Bace^UAS.cCSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Carmine-Simmen

Secondary FlyBase IDs

References (9)

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