FlyBase Allele Report: Dmel\Nep1[EY21255]

General Information

Symbol

Dmel\Nep1^EY21255

Species

D. melanogaster

Name

FlyBase ID

FBal0192619

Feature type

allele

Associated gene

Dmel\Nep1

Associated Insertion(s)

P{EPgy2}Nep1^EY21255

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Bellen, 2006.8.3)

Progenitor genotype

Associated Insertion(s)

P{EPgy2}Nep1^EY21255

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{EPgy2}

misexpression element

Regulatory region(s)

UASp

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:6,093,848..6,093,848 [+]

(Turrel et al., 2017, Panz et al., 2012, Bellen, 2006.8.3)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Nep1^EY21255 under the control of either Scer\GAL4^c739 or Scer\GAL4^VT064246 (using tub-Gal80[ts] to limit the expression to adulthood) does not impair middle-term memory tested in an aversive olfactory memory assay 2hr post-training.

(Turrel et al., 2017)

Animals expressing Nep1^EY21255 under the control of Scer\GAL4^Mef2.5xCD develop into adults without any obvious defects.

(Panz et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Nep1^EY21255, Scer\GAL4^VT064246 is a suppressor of abnormal memory | adult stage phenotype of Appl^{Aβ.UAS.Tag:HA}, Scer\GAL4^VT064246

(Turrel et al., 2017)

Scer\GAL4^VT064246, Nep1^EY21255 is a suppressor of abnormal memory | adult stage phenotype of Appl^UAS.cTa, Bace^UAS.cCSa, Scer\GAL4^VT064246

(Turrel et al., 2017)

NOT Suppressor of

Statement

Reference

Nep1^EY21255, Scer\GAL4^c739 is a non-suppressor of abnormal memory | adult stage phenotype of Appl^{Aβ.UAS.Tag:HA}, Scer\GAL4^c739

(Turrel et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Flies simultaneously expressing Appl^Scer\UAS.cTa and Bace^{Scer\UAS.cCSa} under the control of Scer\GAL4^VT064246 display deficit in both middle- and long-term memory and this can be rescued by co-expression of Nep1^EY21255.

The impaired long-term memory tested in an aversive olfactory memory assay (measured 24hr post-training) characteristic for adult flies expressing Appl^{Aβ.Scer\UAS.T:Ivir\HA1} (combined with tub-Gal80[ts] to limit expression to adulthood) under the control of either Scer\GAL4^c739 or Scer\GAL4^VT064246 is not significantly affected by co-expression of Nep1^EY21255 with the Scer\GAL4^c739 driver but is effectively rescued with the Scer\GAL4^VT064246 driver.

(Turrel et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

22465

y¹ w^67c23 P{EPgy2}Nep1^EY21255

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Nep1^EY21255

(Turrel et al., 2017, Bellen, 2006.8.3)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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