FlyBase Allele Report: Dmel\Appl[Aβ.UAS.Tag:HA]

General Information

Symbol

Dmel\Appl^{Aβ.UAS.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0221089

Feature type

allele

Associated gene

Dmel\Appl

Associated Insertion(s)

Carried in Construct

P{UAS-Appl.Aβ}

Also Known As

UAS-dAβ

Key Links

Transgenic product class

inframe_deletion

(Carmine-Simmen et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Carmine-Simmen et al., 2009)

Mutagen

in vitro construct

(Carmine-Simmen et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-Appl.Aβ}

Cytology

Description

UASt regulatory sequences drive expression of Appl amino acid residues 767-831, which are tagged with Tag:HA.

(Carmine-Simmen et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Appl

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Alzheimer's disease

CEA

(Turrel et al., 2017, Carmine-Simmen et al., 2009)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory | adult stage, with Scer\GAL4^c739

(Turrel et al., 2017)

abnormal memory | adult stage, with Scer\GAL4^VT064246

(Turrel et al., 2017)

abnormal memory | adult stage | RU486 conditional, with Appl^d, Scer\GAL4^{Mef2.247.Switch}

(Bourdet et al., 2015)

Phenotype Manifest In

adult brain, with Scer\GAL4^Appl.G1a

(Carmine-Simmen et al., 2009)

retina, with Scer\GAL4^GMR.PF

(Carmine-Simmen et al., 2009)

Detailed Description

Statement

Reference

Expression of Appl^{Aβ.Scer\UAS.T:Ivir\HA1} under the control of either Scer\GAL4^c739 or Scer\GAL4^VT064246 (using tub-Gal80[ts] to limit the expression to adulthood) does not impair middle-term memory (measured 2hr post-training) but strongly reduces long-term memory (measured 24hr after training) tested in an aversive olfactory memory assay.

(Turrel et al., 2017)

Adult specific expression of Appl^{Aβ.Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^{Mef2.247.Switch} (activated in adult flies fed RU486) does not significantly affect short term memory in an olfactory aversive conditioning paradigm, compared to controls.

Adult specific expression of Appl^{Aβ.Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^{Mef2.247.Switch} (activated in adult flies fed RU486) significantly enhances short term memory defects in Appl^d/+ flies (compared to Appl^d/+ flies or to controls not fed RU486); there are no significant effects on learning, olfactory acuity or shock reactivity.

(Bourdet et al., 2015)

30 day old flies expressing Appl^{Aβ.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF have aggregates in the retina (these aggregates are not seen in wild-type controls). These aggregates have characteristics of amyloid deposits. These 30 day old flies also show extensive vacuolisation of the retina.

Expression of Appl^{Aβ.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Appl.G1a results in the presence of toluidine blue-positive deposits, vacuoles and dark shrunken nuclei in the brains of 30 day old flies. No gross abnormalities are seen in these flies at 2 days after eclosion.

6 day old flies expressing Appl^{Aβ.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav-C155 have a significantly lower performance index in a fast phototaxis assay than control flies and the performance continues to decline with age in the mutant flies.

10 day old flies expressing Appl^{Aβ.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Appl.G1a have fibrillar structures within the axons.

(Carmine-Simmen et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Appl^{Aβ.UAS.Tag:HA}, Scer\GAL4^VT064246 has abnormal memory | adult stage phenotype, suppressible by Nep1^EY21255, Scer\GAL4^VT064246

(Turrel et al., 2017)

NOT suppressed by

Statement

Reference

Appl^{Aβ.UAS.Tag:HA}, Scer\GAL4^c739 has abnormal memory | adult stage phenotype, non-suppressible by Nep1^EY21255, Scer\GAL4^c739

(Turrel et al., 2017)

Appl^{Aβ.UAS.Tag:HA}, Scer\GAL4^VT064246 has abnormal memory | adult stage phenotype, non-suppressible by Nep1^mut.UAS, Scer\GAL4^VT064246

(Turrel et al., 2017)

Enhancer of

Statement

Reference

Appl^{Aβ.UAS.Tag:HA}, Scer\GAL4^VT064246 is an enhancer of abnormal memory | adult stage phenotype of Nep1^GD11797, Scer\GAL4^VT064246

(Turrel et al., 2017)

NOT Enhancer of

Statement

Reference

Appl^{Aβ.UAS.Tag:HA}, Scer\GAL4^c739 is a non-enhancer of abnormal memory | adult stage phenotype of Nep1^GD11797, Scer\GAL4^c739

(Turrel et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The impaired long-term memory tested in an aversive olfactory memory assay (measured 24hr post-training) characteristic for adult flies expressing Appl^{Aβ.Scer\UAS.T:Ivir\HA1} (combined with tub-Gal80[ts] to limit expression to adulthood) under the control of either Scer\GAL4^c739 or Scer\GAL4^VT064246 is not significantly affected by co-expression of Nep1^EY21255 with the Scer\GAL4^c739 driver but is effectively rescued with the Scer\GAL4^VT064246 driver, whereas co-expression of Nep1^mut.Scer\UAS (with Scer\GAL4^VT064246) has no effect.

Adult flies expressing both Nep1^KK101227 and Appl^{Aβ.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^c739 (combined with tub-Gal80[ts] to limit expression to adulthood) do not show any defects in middle-term memory (MTM) tested in an aversive olfactory memory assay 2hr post-training, whereas with the Scer\GAL4^VT064246 driver, the MTM is significantly reduced.

(Turrel et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Appl^{Aβ.Scer\UAS.T:Ivir\HA1}

Appl^{Aβ.UAS.Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (3)

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