FlyBase Allele Report: Dmel\Appl[UAS.cTa]

General Information

Symbol

Dmel\Appl^UAS.cTa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Torroja

FlyBase ID

FBal0097416

Feature type

allele

Associated gene

Dmel\Appl

Associated Insertion(s)

Carried in Construct

P{UAS-Appl.T}

Also Known As

UAS-APPL

Key Links

Transgenic product class

cDNA

(Torroja et al., 1999)

wild_type

(Torroja et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Torroja et al., 1999)

wild_type

(Torroja et al., 1999)

Mutagen

in vitro construct

(Torroja et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-Appl.T}

Cytology

Description

Expression of a full length Appl cDNA is governed by UAS regulatory sequences.

(Torroja et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Appl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon | larval stage, with Scer\GAL4^ppk.PU

(Pizzano et al., 2023)

bouton | ectopic, with Scer\GAL4^unspecified

(Torroja et al., 1999)

crossvein, with Scer\GAL4^Bx-MS1096

(Kim et al., 2007)

dense core granule | decreased number | male limited, with Scer\GAL4^dsx.KI

(Singh et al., 2025)

larval multidendritic class IV neuron, with Scer\GAL4^ppk.PU

(Pizzano et al., 2023)

macrochaeta, with Scer\GAL4^ap-md544

(Kim et al., 2007)

male accessory gland secondary cell, with Scer\GAL4^dsx.KI

(Singh et al., 2025)

ventral adult lateral neuron & dendritic tree, with Scer\GAL4^P2.4.Pdf

(Leyssen et al., 2005)

wing, with Scer\GAL4^Bx-MS1096

(Kim et al., 2007)

Detailed Description

Statement

Reference

Expressing Appl^UAS.cTa under the control of Scer\GAL4^GMR91H05 does not lead to pruning or synaptic transmission defects (10 trains of 10 stimulations at 100 Hz) in adult giant fibers.

(Penserga et al., 2019)

Expression of Appl^Scer\UAS.cTa under the control of Scer\GAL4^VT064246 (using tub-Gal80[ts] to limit the expression to adulthood) does not impair either middle- or long-term memory compared to controls (tested in an aversive olfactory memory assay 2hr and 24hr post-training, respectively).

(Turrel et al., 2017)

Expression of wild-type Appl^Scer\UAS.cTa in the mushroom bodies, under the RU-fed control of Scer\GAL4^{Mef2.247.Switch} does not alter the STM (2hour) of wild-type flies.

(Bourdet et al., 2015)

Expression of one copy of Appl^Scer\UAS.cTa under the control of Scer\GAL4^ap-md544 does not result in a mutant thorax phenotype in adults.

Expression of two copies of Appl^Scer\UAS.cTa under the control of Scer\GAL4^ap-md544 results in loss of thoracic microchaetae.

Expression of one copy of Appl^Scer\UAS.cTa under the control of Scer\GAL4^Bx-MS1096 has no effect on the size of the wing. Expression of two copies of Appl^Scer\UAS.cTa under the control of Scer\GAL4^Bx-MS1096 results in reduced wing size and loss of crossveins.

(Kim et al., 2007)

Expressing Appl^Scer\UAS.cTa, under the control of Scer\GAL4^P2.4.Pdf, has no effect on the early development of the axonal pattern of sLNv neurons. In contrast, this expression results in a large increase in the area of the axonal arbour in adult flies.

(Leyssen et al., 2005)

When Appl^Scer\UAS.cTa is driven by Scer\GAL4^sca-537.4 no phenotype is seen.

(Merdes et al., 2004)

Appl^Scer\UAS.cTa alone, when driven by Scer\GAL4^Appl.G1a, causes 67% viability. The inviable flies do not show the infantile phenotype, in general. Similar phenotypes occur with the Scer\GAL4^elav-C155 driver.

(Torroja et al., 1999)

When expression is driven in the motoneurons extra small boutons of abnormal morphology develop. Many small satellite boutons bud off from a central bouton of normal appearance. Molecular markers suggest that the extra boutons are sufficiently normal to form functional synapses. When expression is driven in the motoneurons muscles 6 and 7 (abdominal segment 3) total number of synaptic boutons is increased, percentage of satellite boutons is increased and the number of normal boutons is increased.

(Torroja et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Appl^UAS.cTa, Scer\GAL4^ap-md544 has increased cell death phenotype, enhanceable by APP-BP1[+]/APP-BP1^Ex62

(Kim et al., 2007)

Appl^UAS.cTa, Scer\GAL4^Appl.G1a has partially lethal - majority die phenotype, enhanceable by Btau\MAPT^UAS.cTa, Scer\GAL4^Appl.G1a

(Torroja et al., 1999)

Appl^UAS.cTa, Scer\GAL4^elav-C155 has partially lethal - majority die phenotype, enhanceable by Btau\MAPT^UAS.cTa, Scer\GAL4^elav-C155

(Torroja et al., 1999)

Appl^UAS.cTa, Scer\GAL4^Appl.G1a has partially lethal - majority die phenotype, enhanceable by Khc⁸

(Torroja et al., 1999)

Appl^UAS.cTa, Scer\GAL4^Appl.G1a has partially lethal - majority die phenotype, enhanceable by Df(2R)Jp6

(Torroja et al., 1999)

NOT Enhanced by

Statement

Reference

Appl^UAS.cTa, Scer\GAL4^ppk.PU has abnormal heat stress response | larval stage phenotype, non-enhanceable by Dscam1^{4.3-6.36-9.25-17.2}, Scer\GAL4^ppk.PU

(Pizzano et al., 2023)

Suppressed by

Statement

Reference

Appl^UAS.cTa, Bace^UAS.cCSa, Scer\GAL4^VT064246 has abnormal memory | adult stage phenotype, suppressible by Nep1^EY21255, Scer\GAL4^VT064246

(Turrel et al., 2017)

APP-BP1[+]/APP-BP1^Ex62, Appl^UAS.cTa, Scer\GAL4^ap-md544 has visible phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^ap-md544

(Kim et al., 2007)

NOT suppressed by

Statement

Reference

Appl^UAS.cTa, Scer\GAL4^ppk.PU has abnormal heat stress response | larval stage phenotype, non-suppressible by Dscam1^{4.3-6.36-9.25-17.2}, Scer\GAL4^ppk.PU

(Pizzano et al., 2023)

Enhancer of

Statement

Reference

Appl^UAS.cTa, Scer\GAL4^ninaE.PT is an enhancer of decreased cell number | adult stage | progressive phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

Appl^UAS.cTa, Scer\GAL4^Appl.G1a is an enhancer of partially lethal - majority die phenotype of Btau\MAPT^UAS.cTa, Scer\GAL4^Appl.G1a

(Torroja et al., 1999)

Appl^UAS.cTa, Scer\GAL4^elav-C155 is an enhancer of partially lethal - majority die phenotype of Btau\MAPT^UAS.cTa, Scer\GAL4^elav-C155

(Torroja et al., 1999)

Other

Statement

Reference

Appl^UAS.cTa, Bace^UAS.cCSa, Scer\GAL4^VT064246 has abnormal memory | adult stage phenotype

(Turrel et al., 2017)

APP-BP1[+]/APP-BP1^Ex62, Appl^UAS.cTa, Scer\GAL4^ap-md544 has visible phenotype

(Kim et al., 2007)

Appl^UAS.cTa, Cul1^EX/lin19[+], Scer\GAL4^ap-md544 has visible phenotype

(Kim et al., 2007)

Phenotype Manifest In

Suppressed by

Statement

Reference

APP-BP1[+]/APP-BP1^Ex62, Appl^UAS.cTa, Scer\GAL4^ap-md544 has scutellum phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^ap-md544

(Kim et al., 2007)

APP-BP1[+]/APP-BP1^Ex62, Appl^UAS.cTa, Scer\GAL4^ap-md544 has macrochaeta phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^ap-md544

(Kim et al., 2007)

Appl^UAS.cTa, Scer\GAL4^Appl.G1a has larval nervous system phenotype, suppressible by robl^K

(Gunawardena and Goldstein, 2001)

Appl^UAS.cTa, Scer\GAL4^Appl.G1a has larval nervous system phenotype, suppressible by Df(3L)GN24

(Gunawardena and Goldstein, 2001)

Enhancer of

Statement

Reference

Appl^UAS.cTa, Scer\GAL4^ninaE.PT is an enhancer of outer photoreceptor cell | adult stage | decreased number phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

Scer\GAL4^Appl.G1a/Appl^UAS.cTa is an enhancer of larval nervous system phenotype of Khc⁸

(Gunawardena and Goldstein, 2001)

Scer\GAL4^Appl.G1a/Appl^UAS.cTa is an enhancer of larval nervous system phenotype of Klc^8ex94

(Gunawardena and Goldstein, 2001)

Suppressor of

Statement

Reference

Scer\GAL4^Appl.G1a/Appl^UAS.cTa is a suppressor of phenotype of Sh¹⁴

(Torroja et al., 1999)

Scer\GAL4^elav-C155/Appl^UAS.cTa is a suppressor of phenotype of Sh¹⁴

(Torroja et al., 1999)

Other

Statement

Reference

APP-BP1[+]/APP-BP1^Ex62, Appl^UAS.cTa, Scer\GAL4^ap-md544 has macrochaeta phenotype

(Kim et al., 2007)

APP-BP1[+]/APP-BP1^Ex62, Appl^UAS.cTa, Scer\GAL4^ap-md544 has scutellum phenotype

(Kim et al., 2007)

Appl^UAS.cTa, Cul1^EX/lin19[+], Scer\GAL4^ap-md544 has macrochaeta phenotype

(Kim et al., 2007)

Appl^UAS.cTa, Cul1^EX/lin19[+], Scer\GAL4^ap-md544 has scutellum phenotype

(Kim et al., 2007)

Appl^UAS.cTa, Scer\GAL4^sca-537.4, numb¹⁵ has eo neuron | somatic clone phenotype

(Merdes et al., 2004)

Appl^UAS.cTa, Scer\GAL4^sca-537.4, numb¹⁵ has trichogen cell | somatic clone phenotype

(Merdes et al., 2004)

Appl^UAS.cTa, Scer\GAL4^sca-537.4, numb¹⁵ has thecogen cell | somatic clone phenotype

(Merdes et al., 2004)

Appl^UAS.cTa, Scer\GAL4^sca-537.4, numb¹⁵ has tormogen cell | ectopic | somatic clone phenotype

(Merdes et al., 2004)

Appl^UAS.cTa, Scer\GAL4^sca-537.4, numb¹⁵ has mechanosensory sensory organ | somatic clone phenotype

(Merdes et al., 2004)

Appl^UAS.cTa, Btau\MAPT^UAS.cTa, Scer\GAL4^Appl.G1a has wing phenotype

(Torroja et al., 1999)

Appl^UAS.cTa, Btau\MAPT^UAS.cTa, Scer\GAL4^Appl.G1a has adult cuticle phenotype

(Torroja et al., 1999)

Appl^UAS.cTa, Btau\MAPT^UAS.cTa, Scer\GAL4^elav-C155 has wing phenotype

(Torroja et al., 1999)

Appl^UAS.cTa, Btau\MAPT^UAS.cTa, Scer\GAL4^elav-C155 has axon phenotype

(Torroja et al., 1999)

Appl^UAS.cTa, Btau\MAPT^UAS.cTa, Scer\GAL4^elav-C155 has adult cuticle phenotype

(Torroja et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Flies simultaneously expressing Appl^Scer\UAS.cTa and Bace^{Scer\UAS.cCSa} under the control of Scer\GAL4^VT064246 display deficit in both middle- and long-term memory and this can be rescued by co-expression of Nep1^EY21255.

The impaired middle- or long-term term memory, MTM or LTM, tested in an aversive olfactory memory assay 2hr or 24hr post-training, respectively, characteristic for adult flies expressing Nep1^GD11797 under the control of either Scer\GAL4^c739 or Scer\GAL4^VT064246 (combined with tub-Gal80[ts] to limit expression to adulthood) is not by enhanced by co-expression of Appl^{Aβ.Scer\UAS.T:Ivir\HA1} with the Scer\GAL4^c739 driver, but significantly worsens the MTM phenotype with the Scer\GAL4^VT064246 driver. Flies simultaneously expressing Nep1^GD11797 and Appl^{Aβ.Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^VT064246 do not show any learning ability defects. Both the MTM and the LTM deficit observed in flies expressing Nep1^GD11797 under Scer\GAL4^VT064246 are exacerbated further by co-expression of Bace^{Scer\UAS.cCSa}.

(Turrel et al., 2017)

Expression of one copy of Appl^Scer\UAS.cTa under the control of Scer\GAL4^ap-md544 in a APP-BP1^Ex62/+ background results in loss of thoracic microchaetae and a reduction in scutellum size. This phenotype is more severe at 29[o]C than at 25[o]C.

Expression of one copy of Appl^Scer\UAS.cTa under the control of Scer\GAL4^ap-md544 in a lin19^EX/+ background results in loss of thoracic microchaetae.

(Kim et al., 2007)

Coexpression of Appl^Scer\UAS.cTa and Psn^+14.Scer\UAS under the control of Scer\GAL4^Act5C.PI does not cause lethality or affect wing patterning.

(Greeve et al., 2004)

When numb¹⁵ somatic mutant clones are made in the thorax of animals expressing Appl^Scer\UAS.cTa (driven by Scer\GAL4^sca-537.4), mechanosensory organs are seen that are made up of four socket cells.

(Merdes et al., 2004)

The organelle clogging phenotype seen in mutant larvae (driven by Scer\GAL4^Appl.G1a) is suppressed by the addition of Df(3L)GN24 or robl^K/+.

The organelle clogging phenotype seen in mutant larvae (Driven by Scer\GAL4^Appl.G1a) is suppressed by the addition of Df(3L)GN24. The organelle clogging phenotype seen in mutant larvae (Driven by Scer\GAL4^Appl.G1a) is suppressed by the addition of robl^K/+.

(Gunawardena and Goldstein, 2001)

Suppresses the leg shaking phenotype of Sh¹⁴ when expression is driven by either Scer\GAL4^Appl.G1a or Scer\GAL4^elav-C155. Suppression is initially complete and starts to decrease on the fourth day after eclosion. Older flies exhibit the non-shaking phenotype for a short time (2-3 minutes) immediately after anesthesia, after which they start to shake at intervals.

(Torroja et al., 1999)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa suppresses the loss of thoracic microchaetae and reduction in scutellum size that is seen in animals expressing one copy of Appl^Scer\UAS.cTa under the control of Scer\GAL4^ap-md544 in a APP-BP1^Ex62/+ background.

(Kim et al., 2007)

37.9% viability when Btau\MAPT^Scer\UAS.cTa is driven by Scer\GAL4^Appl.G1a in combination with Appl^Scer\UAS.cTa. In all cases lethality acts mostly during the pharate adult stage. Eclosing adults show an infantile phenotype where wing expansion and cuticle hardening did not occur. Transport of axonal cargo is reduced in the Btau\MAPT^Scer\UAS.cTa, Appl^Scer\UAS.cTa double mutant.

(Torroja et al., 1999)

Complementation and Rescue Data

Rescues

Scer\GAL4^{Mef2.247.Switch}/Appl^UAS.cTa rescues Appl^d/Appl[+]

(Bourdet et al., 2015)

Partially rescues

Scer\GAL4^sca-537.4/Appl^UAS.cTa partially rescues Appl^d

(Merdes et al., 2004)

Comments

Expression of wild-type Appl^Scer\UAS.cTa in the mushroom bodies, under the RU-fed control of Scer\GAL4^{Mef2.247.Switch} rescues the STM and LTM deficits found in Appl^d/+ ; Scer\GAL4^{Mef2.247.Switch} flies.

(Bourdet et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

38403

w^*; P{UAS-Appl.T}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Appl^Scer\UAS.cTa

Appl^UAS.cTa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Torroja

(Torroja et al., 1999)

Secondary FlyBase IDs

References (18)

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