FlyBase Allele Report: Dmel\fz[30]

fz¹⁵/fz³⁰ transheterozygous mutants exhibit 'rough' eyes. A Fourier transform of the mutant eye image reveals a set of concentric circles reflecting a non-ordered arrangement, instead of distinct reflexes up to the fourth order, as in wild-type. More intense regions arranged as apexes of hexagons, reflecting the existence of small regions of the eye surface with dense hexagonal ommatidial packing, can also be seen in the Fourier transform of these mutants. The diffraction pattern from the cornea of these transheterozygotes is noticeably smeared and only reflexes of the first order arranged in the hexagon apexes are observed. This confirms the limited periodicity of ommatidial arrangement and the existence of only small regions with dense hexagonal packing of ommatidia in the mutant flies.

While the borders of the wild-type ommatidia are tightly aligned to each other, irregular infiltrations of the lens material fill the gap between the lenses of fz¹⁵/fz³⁰ transheterozygous mutants. These infiltrations indicate that the packing of the mutant lenses is less compact, making them more loosely aligned to each other, explaining the 'rough' appearance and the lack of regularity.

The surface of the ommatidial lens at the nano-scale represents the array of nipples with a cross-section of 250nm and height of 30nm. fz¹⁵/fz³⁰ transheterozygous mutant nipples are shorter by 5nm but have the same broadness as those of wild-type flies.

(Kryuchkov et al., 2011)

The directional preferences of growing microtubules in fz³⁰/fz²³ wing cells is not significantly different from that seen in wild type at 24 hours after puparium formation.

(Harumoto et al., 2010)

fz²¹/fz³⁰ stage 16 embryos have longer dorsal trunks than normal.

(Chung et al., 2009)

The planar polarity defects seen in the eyes of animals expressing fz^Scer\UAS.cAa under the control of Scer\GAL4^hs.2sev are almost completely suppressed by fz³⁰ (76.0% of ommatidia are correctly rotated).

(Chung et al., 2007)

fz³⁷/fz³⁰ show defects in the orientation of the mitotic spindle in dividing pI sensory precursor cells.

(David et al., 2005)

In fz³⁰/fz³⁷ pupae, the mitotic spindles of dividing sensory organ precursor cells in the developing notum are not aligned with the anterior-posterior axis, but instead are randomly oriented.

(Bellaiche et al., 2004)

In fz³⁷/fz³⁰ mutant pupae, unlike wild-type, the pI cells exhibit almost no centrosome rotation before spindle formation. Complete rotation values for the spindle are similar to wild-type, however in 7% of mutant pI cells, the spindle rotates in one direction before rotating in the opposite direction, bidirectional movements are not seen in wild-type. The spindle does not line up properly with the pon crescent, leading to defective partitioning of pon. 5% of fz³⁷/fz³⁰ flies have at least one bristle with two shafts and two sockets on the notum.

(Bellaiche et al., 2001)

Wings have an abnormal planar tissue polarity pattern in fz³⁰/Df(3L)fz-D21 animals.

(Rulifson et al., 2000)

Embryos lacking maternal and zygotic fz function (derived from a cross between fz³⁰/fz²³ males and females) are missing the RP2/sib lineage in 1-3 hemisegments in 15% of cases. Row 6 neuroblasts are infrequently missing in a partially penetrant manner. Embryos lacking maternal and zygotic fz function (derived from a cross between fz¹/fz³⁰ males and females) are missing the RP2/sib lineage in 1-2 hemisegments in 10% of cases.

(Bhat, 1998)

In fz³/fz³⁷ transheterozygotes the socket, shaft and pIIb cells are always localised in the region of the cell in contact with pIIb. The socket cells always occupies an eccentric position.

(Gho and Schweisguth, 1998)

Clones in adult wings cause wild type hairs distal to and in part anterior and posterior to the clone displaying abnormal polarity have a strong bias to point towards, rather than away from the clone. Clones in differentiating pupal wings show the same phenotype. This is referred to as an 'attractive' non-cell-autonomous effect. Wild type hairs at the proximal edge of the clone are usually rescued.

(Adler et al., 1997)

fz³⁰/Df(3L)fz-D21 mutants carrying Dvir\fz^hs.PJ and raised at 29^oC are substantially rescued for the wing polarity and multiple hair defects. Dvir\fz^hs.PJ produces a gain of function phenotype similar to that caused by fz^I.hs.

(Jones et al., 1996)

Strong phenotype.

(Adler et al., 1990)

Strong thoracic bristle phenotype; strong wing-hair disorientation.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

fz[+]/fz³⁰ is a non-enhancer of abnormal neuroanatomy phenotype of DAAM^Ex1

(Gombos et al., 2015)

fz³⁰ is a non-enhancer of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

Suppressor of

Statement

Reference

fz²¹/fz³⁰ is a suppressor of abnormal planar polarity phenotype of Scer\GAL4^ptc.PU, fy^UAS.GFP, in^UAS.Tag:HA

(Wang et al., 2014)

fz³⁰ is a suppressor of abnormal cell polarity phenotype of pk^pk.sev

(Jenny et al., 2003)

fz³⁰ is a suppressor | partially of abnormal cell polarity phenotype of Scer\GAL4^hs.2sev, pk^sple.UAS

(Jenny et al., 2003)

NOT Suppressor of

Statement

Reference

fz³⁰ is a non-suppressor of visible phenotype of upd1^GMR.PB

(Bach et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

fz³⁷/fz³⁰ has eo neuron phenotype, enhanceable by numb⁷⁹⁶

(Bellaiche et al., 2001)

fz³⁷/fz³⁰ has thecogen cell phenotype, enhanceable by numb⁷⁹⁶

(Bellaiche et al., 2001)

Enhancer of

Statement

Reference

fz²³/fz³⁰ is an enhancer of wing hair phenotype of fy²/fy⁶

(Lee and Adler, 2002)

fz³⁰/fz²⁵ is an enhancer of wing hair phenotype of fy¹/fy⁶

(Lee and Adler, 2002)

fz[+]/fz³⁰ is an enhancer of first basal wing cell phenotype of Vang^A3

(Taylor et al., 1998)

fz[+]/fz³⁰ is an enhancer of first basal wing cell phenotype of Vang^Tbs42

(Taylor et al., 1998)

NOT Enhancer of

Statement

Reference

fz[+]/fz³⁰ is a non-enhancer of adult mushroom body alpha-lobe phenotype of DAAM^Ex1

(Gombos et al., 2015)

fz[+]/fz³⁰ is a non-enhancer of adult mushroom body beta-lobe phenotype of DAAM^Ex1

(Gombos et al., 2015)

fz³⁰ is a non-enhancer of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

fz³⁰ is a non-enhancer of ommatidium phenotype of Scer\GAL4^sev.PM181, stan^UAS.cUa

(Das et al., 2002)

fz³⁰ is a non-enhancer of photoreceptor cell R3 | ectopic phenotype of Scer\GAL4^sev.PM181, stan^UAS.cUa

(Das et al., 2002)

fz³⁰ is a non-enhancer of photoreceptor cell R4 phenotype of Scer\GAL4^sev.PM181, stan^UAS.cUa

(Das et al., 2002)

Suppressor of

Statement

Reference

fz³⁰ is a suppressor of ommatidium phenotype of pk^pk.sev

(Jenny et al., 2003)

fz³⁰ is a suppressor | partially of ommatidium phenotype of Scer\GAL4^hs.2sev, pk^sple.UAS

(Jenny et al., 2003)

fz³⁰ is a suppressor of phenotype of Vang^A3

(Taylor et al., 1998)

fz³⁰ is a suppressor of phenotype of Vang^Tbs42

(Taylor et al., 1998)

NOT Suppressor of

Statement

Reference

fz³⁰ is a non-suppressor of eye phenotype of upd1^GMR.PB

(Bach et al., 2003)

fz³⁰ is a non-suppressor of ommatidium phenotype of Scer\GAL4^sev.PM181, stan^UAS.cUa

(Das et al., 2002)

fz³⁰ is a non-suppressor of photoreceptor cell R3 | ectopic phenotype of Scer\GAL4^sev.PM181, stan^UAS.cUa

(Das et al., 2002)

fz³⁰ is a non-suppressor of photoreceptor cell R4 phenotype of Scer\GAL4^sev.PM181, stan^UAS.cUa

(Das et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of fz³⁰ does not enhance the axonal projection defects seen in the α and β lobes of DAAM^Ex1 mutant mushroom bodies.

(Gombos et al., 2015)

The characteristic swirl of wing hairs seen on the ventral surface of the wing in animals co-expressing fy^{Scer\UAS.T:Avic\GFP} and in^{Scer\UAS.T:Ivir\HA1} under the control of two copies of Scer\GAL4^ptc.PU is not seen in a fz²¹/fz³⁰ background.

(Wang et al., 2014)

The proportion of symmetrical (R3/R3) ommatidia in the eyes of stan^Scer\UAS.cUa; Scer\GAL4^sev.PM181 flies is unaffected by heterozygosity for fz³⁰.

(Das et al., 2002)

The addition of numb⁷⁹⁶ to fz³⁷/fz³⁰, leads to pupae that have sense organs composed of four cells with two socket cells but devoid of either sheath or neuronal cells.

(Bellaiche et al., 2001)

Acts as a dominant enhancer of the dominant wing basal cell 1 phenotype of Vang^Tbs42 and Vang^A3. In double mutant combinations between Vang^A3, Vang^A5 and Vang^Tbs42 with fz¹, fz²⁵ and fz³⁰, the fz-in type of polarity pattern was slightly less abnormal than in either single mutant. The domineering nonautonomy of Vang^A3 and Vang^Tbs42 clones can be suppressed by fz²⁵ and fz³⁰.