FlyBase Allele Report: Dmel\numb[796]

General Information

Symbol

Dmel\numb⁷⁹⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0091225

Feature type

allele

Associated gene

Dmel\numb

Associated Insertion(s)

Carried in Construct

Also Known As

nb⁷⁹⁶

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Buescher et al., 1998)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Yedvobnick et al., 2004)

abnormal neuroanatomy | embryonic stage

(Zhu and Bhat, 2011, Gaziova and Bhat, 2009)

lethal | recessive

(Buescher et al., 1998)

Phenotype Manifest In

Detailed Description

Statement

Reference

The GMC-1 cells in numb⁷⁹⁶ mutant embryos symmetrically divide into two sib cells rather than a sib and an RP2 cell.

(Zhu and Bhat, 2011)

In numb⁷⁹⁶ mutants, the GMC-1 of the RP2/sib lineage symmetrically divides into two sib cells instead of an RP2 and a sib. Penetrance is restricted to ~50% of hemisegments.

(Gaziova and Bhat, 2009)

GMC-1 of the RP2/sib lineage divides symmetrically to produce two RP2sib cells in mutant embryos.

(Yedvobnick et al., 2004)

The Malpighian tubules do not take up their normal positions in numb⁷⁹⁶ mutant embryos and the tubules lack tip cells.

(Ainsworth et al., 2000)

In mutant embryos Malpighian tubule tip cells are missing except in about 8% embryos, where one or two of the four tubules are allocated normally. However, there is no change in the final number of tubule cells. The distinction between the number of cells in the anterior and posterior tubules is also unaffected.

(Wan et al., 2000)

In about 27% of hemisegments in mutant embryos, a RP2 to RP2sib transformation is seen. This division produces two cells of unequal size, but both cells behave like sib neurons.

(Wai et al., 1999)

27% of mutant hemisegments show an RP2 to RP2sib cell fate transformation. The number of eve-positive CQ neurons is increased. 11% of hemisegments show an RP2 to RP2sib cell fate transformation in numb¹/numb⁷⁹⁶ embryos. 62% of hemisegments show an RP2 to RP2sib cell fate transformation in numb⁷⁹⁶/Df(2L)30A-C embryos.

(Buescher et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Hem^J4-48, numb⁷⁹⁶ has abnormal neuroanatomy | embryonic stage phenotype

(Zhu and Bhat, 2011)

mid^los1, numb⁷⁹⁶ has abnormal neuroanatomy | embryonic stage phenotype

(Gaziova and Bhat, 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

numb⁷⁹⁶ has ganglion mother cell GMC4-2a phenotype, enhanceable by CycA⁰⁴³³⁵

(Wai et al., 1999)

numb⁷⁹⁶ has larval RP2 motor neuron phenotype, enhanceable by CycA⁰⁴³³⁵

(Wai et al., 1999)

numb⁷⁹⁶ has larval DA1 motor neuron | precursor phenotype, enhanceable by CycA⁰⁴³³⁵

(Wai et al., 1999)

numb⁷⁹⁶ has pCC neuron | precursor phenotype, enhanceable by CycA⁰⁴³³⁵

(Wai et al., 1999)

Enhancer of

Statement

Reference

numb⁷⁹⁶ is an enhancer of eo neuron phenotype of fz³⁷/fz³⁰

(Bellaiche et al., 2001)

numb⁷⁹⁶ is an enhancer of thecogen cell phenotype of fz³⁷/fz³⁰

(Bellaiche et al., 2001)

Suppressor of

Statement

Reference

numb⁷⁹⁶/numb⁷⁹⁶ is a suppressor of larval RP2 motor neuron | ectopic phenotype of pdm2^hs.P

(Bhat and Apsel, 2004)

Other

Statement

Reference

Hem^J4-48, numb⁷⁹⁶ has larval RP2 motor neuron | embryonic stage phenotype

(Zhu and Bhat, 2011)

Hem^J4-48, numb⁷⁹⁶ has RP2sib neuron | embryonic stage phenotype

(Zhu and Bhat, 2011)

N^l1N-ts1, numb⁷⁹⁶ has larval RP2 motor neuron phenotype

(Wai et al., 1999)

N^l1N-ts1, numb⁷⁹⁶ has RP2sib neuron phenotype

(Wai et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Embryos double mutant for insc²² and Hem^J4-48 display hemisegments containing two sib neurons and no RP2 neurons.

(Zhu and Bhat, 2011)

In mid^los1, numb⁷⁹⁶ mutants, two ectopic RP2 sib cells are observed with complete penetrance.

(Gaziova and Bhat, 2009)

Heat-shocked, stage 14 pdm2^hs.P; numb⁷⁹⁶/numb⁷⁹⁶ embryos have extra RP2 sibs, but not extra RP2 neurons.

(Bhat and Apsel, 2004)

Both the daughter cells of the GMC-1 of the RP2/sib lineage adopt an RP2 fate in mam⁹ numb⁷⁹⁶ double mutant embryos, indicating that mam is epistatic to numb.

(Yedvobnick et al., 2004)

The addition of numb⁷⁹⁶ to fz³⁷/fz³⁰, leads to pupae that have sense organs composed of four cells with two socket cells but devoid of either sheath or neuronal cells.

(Bellaiche et al., 2001)

In a numb⁷⁹⁶; N^l1N-ts1 double mutant the sib cell is usually transformed into an RP2 neuron, as in N^l1N-ts1 alone. However, this phenotype is only partially penetrant, as some hemisegments are seen with the numb transformation (RP2 to sib). In about 35% of hemisegments in numb⁷⁹⁶; CycA⁰⁴³³⁵ double mutants, the GMC of the RP2/sib lineage fails to divide, and assumes the identity of the sib neuron. However, the GMC of the aCC/pCC lineage fails to divide and assumes the identity of the aCC, as in CycA⁰⁴³³⁵ mutants alone.

(Wai et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

numb⁷⁹⁶/numb¹ is rescued by numb^+t19.5

(Rebeiz et al., 2011)

Df(2L)30A-C/numb⁷⁹⁶ is rescued by numb^+t19.5

(Rebeiz et al., 2011)

Partially rescued by

numb⁷⁹⁶/numb¹ is partially rescued by numb^2del

(Rebeiz et al., 2011)

Df(2L)30A-C/numb⁷⁹⁶ is partially rescued by numb^2del

(Rebeiz et al., 2011)

Comments

numb^+t19.5 efficiently rescues the lethality associated with numb¹/numb⁷⁹⁶ and numb⁷⁹⁶/Df(2L)30A-C. Adult mechanosensory organ development is almost completely normal in the rescued flies.

numb¹/numb⁷⁹⁶ and numb⁷⁹⁶/Df(2L)30A-C flies rescued by numb^2del show a widespread "double socket" phenotype, in which the shaft cell is transformed to a second socket cell.

(Rebeiz et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

nb⁷⁹⁶

(Zhu and Bhat, 2011, Yedvobnick et al., 2004, Udolph et al., 2001, Wan et al., 2000, Wai et al., 1999, Buescher et al., 1998)

numb⁷⁹⁶

(Shan et al., 2018, Rebeiz et al., 2011, Gaziova and Bhat, 2009)

Name Synonyms

Secondary FlyBase IDs

References (15)

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