Larvae expressing Chro^KK105370 under the control of Scer\GAL4^insc-Mz1407 display central brain neural stem cell (NCS) reactivation defects: in contrast to wild-type, in the knockdown larvae at 96h after larval hatching (ALH) almost a third of the mira-positive central brain neural NSCs still extend their processes and only 1% of the dpn-positive cells are actively dividing. Expression of Chro^KK105370 for two days after all the neuroblast have already reactivated (using tub-gal80[ts] to limit the time of expression) is sufficient to induce re-entry to the quiescent state in vast majority of NSCs.

Scer\GAL4^insc-Mz1407-driven expression of Chro^KK105370 does not affect NCS size or specification and neither does it induce excessive apoptosis or premature differentiation in the central brain neuroblasts. However, in contrast to controls, almost half of the neuroblasts show only one centriole, suggesting that they are in either G0 or early G1 stage of the cell cycle.

No defect in exiting the quiescent state is observed in the mushroom body neuroblasts at 12h ALH (as in wild-type controls, the cells are mitotically active and do not have extensions) and at 36h ALH, just as in controls, they continue to proliferate in the absence of dietary amino acids. There are also no indications of any cell cycle defects in these neuroblasts.

Scer\GAL4^ey-OK107-driven expression of Chro^KK105370 leads to formation of shorter mitotic spindle in the mushroom body neuroblasts, but none of these display cellular mira-positive extensions and all are actively dividing cells. Expression controlled by any of the following drivers: Scer\GAL4^repo, Scer\GAL4^Cg.PA or Scer\GAL4^Lsp2.PH does not lead to any neuroblast reactivation defects.

Expression of Chro^KK105370 under the control of Scer\GAL4^Bx-MS1096 results in wings which are reduced in size, severely blistered and misshapen.

Chro^KK105370, Scer\GAL4^insc-Mz1407 has neuroblast | third instar larval stage phenotype, non-suppressible by CycB^UAS.cWa, Scer\GAL4^insc-Mz1407

Chro^KK105370, Scer\GAL4^insc-Mz1407 has neuroblast | third instar larval stage phenotype, non-suppressible by CycE^UAS.cUa, Scer\GAL4^insc-Mz1407

Chro^KK105370, Scer\GAL4^insc-Mz1407 is a suppressor of neuroblast | third instar larval stage | nutrition conditional phenotype of InR^A1325D.UAS, Scer\GAL4^insc-Mz1407

Chro^KK105370, Scer\GAL4^insc-Mz1407 is a suppressor of neuroblast | third instar larval stage | nutrition conditional phenotype of Rheb^UAS.cPa, Scer\GAL4^insc-Mz1407

Chro^KK105370, Scer\GAL4^insc-Mz1407 is a suppressor of neuroblast | third instar larval stage | nutrition conditional phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^insc-Mz1407