FlyBase Allele Report: Dmel\CycB[UAS.cWa]

General Information

Symbol

Dmel\CycB^UAS.cWa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Weiss

FlyBase ID

FBal0086881

Feature type

allele

Associated gene

Dmel\CycB

Associated Insertion(s)

Carried in Construct

P{UAS-CycB.W}

Also Known As

UAS-CycB

Key Links

Transgenic product class

cDNA

(Weiss et al., 1998)

wild_type

(Weiss et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Weiss et al., 1998)

wild_type

(Weiss et al., 1998)

Mutagen

in vitro construct

(Weiss et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-CycB.W}

Cytology

Description

UASt regulatory sequences drive expression of a cDNA fragment that encodes the complete CycB open reading frame.

(Weiss et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

CycB

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Sertoli cell-only syndrome

modeled by bel^neo30, bel⁶

(Kotov et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable | embryonic stage, with Scer\GAL4^da.G32

(Leismann et al., 2000)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of CycB^Scer\UAS.cWa driven by Scer\GAL4^nos.PG does not significantly affect size and architecture of the testes and germline stem cell maintenance and male fertility is normal.

(Kotov et al., 2016)

Scer\GAL4^F4-mediated expression does not affect endoreplication.

(Weiss et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

CycB^UAS.cWa, Scer\GAL4^nanos.PG is a suppressor | partially of male semi-sterile phenotype of Scer\GAL4^nanos.PG, bel^GD1324

(Kotov et al., 2016)

Phenotype Manifest In

Enhancer of

Statement

Reference

CycB^UAS.cWa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Khurana et al., 2006)

NOT Suppressor of

Statement

Reference

CycB^UAS.cWa, Scer\GAL4^insc-Mz1407 is a non-suppressor of neuroblast | third instar larval stage phenotype of Chro^KK105370, Scer\GAL4^insc-Mz1407

(Li et al., 2017)

Other

Statement

Reference

CycB^UAS.cWa, Scer\GAL4^nanos.PG, bel^GD1324 has male germline cell | adult stage phenotype

(Kotov et al., 2016)

CycB^UAS.cWa, Scer\GAL4^nanos.PG, bel^GD1324 has testis | adult stage phenotype

(Kotov et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The reactivation defects in central brain neuroblasts (high proportion of the cells retain the cellular extensions, one of the hallmarks of the quiescent state) observed in third instar larvae expressing Chro^KK105370 under the control of Scer\GAL4^insc-Mz1407 cannot be rescued by co-expression of CycB^Scer\UAS.cWa.

(Li et al., 2017)

Co-expression of CycB^Scer\UAS.cWa and bel^GD1324 driven by Scer\GAL4^nos.PG leads to two distinct phenotypes in testes: most (around 75%) are reduced in size and do not contain germ cells, while the remainder have normal testis architecture and germ cell content; male infertility is significantly partially suppressed by co-expression of CycB^Scer\UAS.cWa in Scer\GAL4^nos.PG>bel^GD1324 males.

(Kotov et al., 2016)

Xenogenetic Interactions

Statement

Reference

The rough-eye phenotype observed in animals expressing Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced by co-expression of CycB^Scer\UAS.cWa.

(Khurana et al., 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CycB^Scer\UAS.cWa

CycB^UAS.cWa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Weiss

(Weiss et al., 1998)

Secondary FlyBase IDs

References (8)

Report Sections

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