FlyBase Allele Report: Dmel\Madm[GD7155]

Expression of Madm^GD7155 under the control of Scer\GAL4^esg-NP5130 (in combination with a Gal80[ts] transgene to restrict expression to the adult stage) results in defects in the adult midgut, including a reduction in the number of mitotic cells, decreased numbers of intestinal stem cells and enteroblasts and remaining cells were abnormally round-shaped; these flies also exhibit reduced numbers of enteroendocrine cells, but do not exhibit defects in the normal intestinal stem cell-enteroblast asymmetry, nor any significant change in the size of intestinal stem cells, as compared to controls.

Expression of Madm^GD7155 under the control of Scer\GAL4^Dl.PU (in combination with a Gal80[ts] transgene to restrict expression to the adult stage) results in a significant reduction in the number of mitotic cells, as compared with controls.

(Nie et al., 2015)

Adults expressing Madm^GD7155 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing Madm^GD7155 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in planar polarity defects in the orientation of bristles of the notum in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

7155

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Madm^GD7155, Scer\GAL4^NP5130 has decreased occurrence of cell division | adult stage phenotype, non-suppressible by BacA\p35^UAS.cUa, Scer\GAL4^NP5130

(Nie et al., 2015)

Suppressor of

Statement

Reference

Madm^GD7155, Scer\GAL4^NP5130 is a suppressor of increased cell size | adult stage phenotype of Scer\GAL4^NP5130, gig^GD1454

(Nie et al., 2015)

Madm^GD7155, Scer\GAL4^NP5130 is a suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP5130, hpo^JF02740

(Nie et al., 2015)

Madm^GD7155, Scer\GAL4^NP5130 is a suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP5130, msn^JF03219

(Nie et al., 2015)

Madm^GD7155, Scer\GAL4^NP5130 is a suppressor of increased occurrence of cell division | adult stage phenotype of InR^A1325D.UAS, Scer\GAL4^NP5130

(Nie et al., 2015)

Other

Statement

Reference

Dysb^e01028, Madm^GD7155, Scer\GAL4^elav-C155 has abnormal neurophysiology | larval stage phenotype

(Mushtaq et al., 2022)

Madm^GD7155, Scer\GAL4^NP5130, bun^{A.UAS.N.Tag:SBP} has increased occurrence of cell division | adult stage phenotype

(Nie et al., 2015)

Madm^GD7155, Scer\GAL4^NP5130, bun^A.UAS has increased occurrence of cell division | adult stage phenotype

(Nie et al., 2015)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Madm^GD7155, Scer\GAL4^NP5130 has adult midgut phenotype, non-suppressible by BacA\p35^UAS.cUa, Scer\GAL4^NP5130

(Nie et al., 2015)

Suppressor of

Statement

Reference

Madm^GD7155, Scer\GAL4^NP5130 is a suppressor of adult midgut phenotype of Scer\GAL4^NP5130, hpo^JF02740

(Nie et al., 2015)

Madm^GD7155, Scer\GAL4^NP5130 is a suppressor of adult midgut phenotype of Scer\GAL4^NP5130, msn^JF03219

(Nie et al., 2015)

Madm^GD7155, Scer\GAL4^NP5130 is a suppressor of adult midgut phenotype of Scer\GAL4^NP5130, gig^GD1454

(Nie et al., 2015)

Madm^GD7155, Scer\GAL4^NP5130 is a suppressor of intestinal stem cell | adult stage phenotype of Scer\GAL4^NP5130, gig^GD1454

(Nie et al., 2015)

Other

Statement

Reference

Dysb^e01028, Madm^GD7155, Scer\GAL4^elav-C155 has embryonic/larval neuromuscular junction | larval stage phenotype

(Mushtaq et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Madm^GD7155 suppresses the increased intestinal stem cell size seen in flies expressing gig^GD1454 under the control of Scer\GAL4^esg-NP5130.

Co-expression of Madm^GD7155 suppresses the increased number of mitotic cells seen in the midguts of flies expressing msn^JF03219, vn^Scer\UAS.cUa, upd3^{Scer\UAS.P\T.cZa}, hpo^JF02740, or Egfr^{U.A887T.Scer\UAS} under the control of Scer\GAL4^esg-NP5130.

(Nie et al., 2015)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cUa does not rescue the decreased cell proliferation observed in the midguts of flies expressing Madm^GD7155 under the control of Scer\GAL4^esg-NP5130.

(Nie et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v27347

w¹¹¹⁸; P{GD7155}v27347

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

7155

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Mad^RNAi

(Nie et al., 2015)

Madm^GD7155

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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