FlyBase Allele Report: Dmel\gig[GD1454]

General Information

Symbol

Dmel\gig^GD1454

Species

D. melanogaster

Name

FlyBase ID

FBal0209637

Feature type

allele

Associated gene

Dmel\gig

Associated Insertion(s)

Carried in Construct

P{GD1454}

Also Known As

UAS-TSC2^RNAi, UAS-tsc2 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1454}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1454

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult midgut, with Scer\GAL4^NP5130

(Nie et al., 2015)

chaeta | increased number, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

intestinal stem cell | adult stage, with Scer\GAL4^NP5130

(Nie et al., 2015)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

spermatocyte, with Scer\GAL4^VP16.bam

(Azuma et al., 2021)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^nub-AC-62

(Resnik-Docampo and de Celis, 2011)

Detailed Description

Statement

Reference

Expression of gig^GD1454 under the control of Scer\GAL4^esg-NP5130 (in combination with a Gal80[ts] transgene to restrict expression to the adult stage) in the midgut results in a significant increase in size of intestinal stem cells.

(Nie et al., 2015)

Expression of gig^GD1454 using Scer\GAL4^nub-AC-62 results in abnormally large wings in which cell size and cell number are increased.

(Resnik-Docampo and de Celis, 2011)

Adults expressing gig^GD1454 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in a weak flyer phenotype.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in extra bristles on the notum in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 0% or 30-40% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 0% or 50-60% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 10-20% or 50-60% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 10-20% or 40-50% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in an enlarged notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1454

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^NP5130, gig^GD1454 has increased cell size | adult stage phenotype, suppressible by bun^GD1392/Scer\GAL4^NP5130

(Nie et al., 2015)

Scer\GAL4^NP5130, gig^GD1454 has increased cell size | adult stage phenotype, suppressible by Madm^GD7155, Scer\GAL4^NP5130

(Nie et al., 2015)

Scer\GAL4^nub-AC-62, gig^GD1454 has abnormal size phenotype, suppressible by Scer\GAL4^nub-AC-62/hppy^UAS.cMa

(Resnik-Docampo and de Celis, 2011)

Suppressor of

Statement

Reference

gig^GD1454/Scer\GAL4^mirr-DE is a suppressor of decreased occurrence of cell division | somatic clone | third instar larval stage phenotype of smo³

(Kim et al., 2017)

NOT Suppressor of

Statement

Reference

gig^GD1454, Scer\GAL4^NP0001 is a non-suppressor of increased cell size phenotype of Atg9^HMS01246, Scer\GAL4^NP0001

(Wen et al., 2017)

Scer\GAL4^salm.EPv/gig^GD1454 is a non-suppressor of visible phenotype of Scer\GAL4^salm.EPv, Tcs5^RNAi.UAS.462

(Ibar et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^NP5130, gig^GD1454 has intestinal stem cell | adult stage phenotype, suppressible by Madm^GD7155, Scer\GAL4^NP5130

(Nie et al., 2015)

Scer\GAL4^NP5130, gig^GD1454 has adult midgut phenotype, suppressible by bun^GD1392/Scer\GAL4^NP5130

(Nie et al., 2015)

Scer\GAL4^NP5130, gig^GD1454 has intestinal stem cell | adult stage phenotype, suppressible by bun^GD1392/Scer\GAL4^NP5130

(Nie et al., 2015)

Scer\GAL4^NP5130, gig^GD1454 has adult midgut phenotype, suppressible by Madm^GD7155, Scer\GAL4^NP5130

(Nie et al., 2015)

Suppressor of

Statement

Reference

gig^GD1454/Scer\GAL4^mirr-DE is a suppressor of eye disc | somatic clone | third instar larval stage phenotype of smo³

(Kim et al., 2017)

NOT Suppressor of

Statement

Reference

gig^GD1454, Scer\GAL4^NP0001 is a non-suppressor of adult midgut epithelium phenotype of Atg9^HMS01246, Scer\GAL4^NP0001

(Wen et al., 2017)

Scer\GAL4^salm.EPv/gig^GD1454 is a non-suppressor of first posterior wing cell phenotype of Scer\GAL4^salm.EPv, Tcs5^RNAi.UAS.462

(Ibar et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The expression of gig^GD1454 (and Dicer-2 transgene, for efficient RNAi) under the control of Scer\GAL4^mirr-DE suppresses the defects of smo³ homozygous somatic clones in third instar larval eye discs to enter S transition during the second mitotic wave.

(Kim et al., 2017)

The co-expression of gig^GD1454 does not suppress the increased midgut cell size resulting from the expression of Atg9^HMS01246 under the control of Scer\GAL4^{Myo31DF-NP0001}.

(Wen et al., 2017)

Co-expression of bun^GD1392 or Madm^GD7155 suppresses the increased intestinal stem cell size seen in flies expressing gig^GD1454 under the control of Scer\GAL4^esg-NP5130.

(Nie et al., 2015)

The reduction in the distance between wing veins 3 and 4 that is seen in animals expressing Prpk^{dsRNA.Scer\UAS.462} under the control of Scer\GAL4^salm.EPv is not suppressed if they are also co-expressing gig^GD1454.

(Ibar et al., 2013)

Co-expression of hppy^Scer\UAS.cMa rescues the large wing phenotype of gig^GD1454, Scer\GAL4^nub-AC-62 flies. Wing size is similar to over-expression of hppy^Scer\UAS.cMa alone.

(Resnik-Docampo and de Celis, 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v6314