FlyBase Allele Report: Dmel\Tcs5[RNAi.UAS.462]

General Information

Symbol

Dmel\Tcs5^RNAi.UAS.462

Species

D. melanogaster

Name

FlyBase ID

FBal0284606

Feature type

allele

Associated gene

Dmel\Tcs5

Associated Insertion(s)

Carried in Construct

P{UAS-Tcs5.RNAi.462}

Key Links

Transgenic product class

RNAi_reagent

(Ibar et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ibar et al., 2013)

Mutagen

in vitro construct

(Ibar et al., 2013)

Progenitor genotype

Carried in construct

P{UAS-Tcs5.RNAi.462}

Cytology

Description

UAS regulatory sequences drive expression of a hairpin of an internal Prpk fragment (462bp).

(Ibar et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Tcs5

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | P-stage, with Scer\GAL4^Hml.Δ

(Molina et al., 2022)

abnormal cell shape, with Scer\GAL4^Cg.PU

(Molina et al., 2022)

abnormal wound healing | larval stage, with Scer\GAL4^Hml.Δ

(Molina et al., 2022)

decreased cell size, with Scer\GAL4^salm.EPv

(Ibar et al., 2013)

increased mortality, with Scer\GAL4^Hml.Δ

(Molina et al., 2022)

visible, with Df(3L)GN24, Scer\GAL4^salm.EPv

(Ibar et al., 2013)

visible, with Scer\GAL4^salm.EPv

(Ibar et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

Larvae expressing Prpk^{dsRNA.Scer\UAS.462} under the control of Scer\GAL4^da.G32 are reduced in size compared to controls. Pupation is inhibited in these animals, but adult escapers are seen, which do not have defects in patterning or differentiation.

Expression of Prpk^{dsRNA.Scer\UAS.462} under the control of Scer\GAL4^salm.EPv results in a clear reduction in the distance between wing veins 3 and 4, due to a reduction in cell size. The phenotype is enhanced if the flies are also carrying Df(3L)GN24.

Clones in the third larval instar wing disc expressing Prpk^{dsRNA.Scer\UAS.462} under the control of Scer\GAL4^Act5C.PU and induced at 60 +/- 12 hours after egg laying are recovered, but they are smaller than control clones, disaggregated and show apoptotic features.

Clones in the fat body expressing Prpk^{dsRNA.Scer\UAS.462} under the control of Scer\GAL4^Act5C.PU and induced 36 +/- 12 hours after egg laying are smaller than control clones at the larval instar stage.

(Ibar et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Cg.PU, Tcs5^RNAi.UAS.462 has abnormal cell shape phenotype, suppressible | partially by Rab35^UASp.YFP, Scer\GAL4^Cg.PU

(Molina et al., 2022)

Scer\GAL4^Cg.PU, Tcs5^RNAi.UAS.462 has abnormal cell shape phenotype, suppressible | partially by Rac1^UAS.cLa, Scer\GAL4^Cg.PU

(Molina et al., 2022)

Scer\GAL4^Cg.PU, Tcs5^RNAi.UAS.462 has abnormal cell shape phenotype, suppressible by Cdc42^V12.UAS, Scer\GAL4^Cg.PU

(Molina et al., 2022)

Scer\GAL4^Hml.Δ, Tcs5^RNAi.UAS.462 has abnormal cell migration | P-stage phenotype, suppressible by Rab35^UASp.YFP, Scer\GAL4^Hml.Δ

(Molina et al., 2022)

Scer\GAL4^Hml.Δ, Tcs5^RNAi.UAS.462 has increased mortality phenotype, suppressible | partially by Rab35^UASp.YFP, Scer\GAL4^Hml.Δ

(Molina et al., 2022)

Scer\GAL4^Hml.Δ, Tcs5^RNAi.UAS.462 has increased mortality phenotype, suppressible | partially by Rac1^UAS.cLa, Scer\GAL4^Hml.Δ

(Molina et al., 2022)

Scer\GAL4^Cg.PU, Tcs5^RNAi.UAS.462 has abnormal cell shape phenotype, suppressible by Mmus\Trp53rka^WT.UAS, Scer\GAL4^Cg.PU

(Molina et al., 2022)

Scer\GAL4^Cg.PU, Tcs5^RNAi.UAS.462 has abnormal cell shape phenotype, suppressible | partially by Mmus\Trp53rka^KD.UAS, Scer\GAL4^Cg.PU

(Molina et al., 2022)

Scer\GAL4^salm.EPv, Tcs5^RNAi.UAS.462 has visible phenotype, suppressible by S6k^STDETE.UAS, Scer\GAL4^salm.EPv

(Ibar et al., 2013)

NOT suppressed by

Statement

Reference

Scer\GAL4^salm.EPv, Tcs5^RNAi.UAS.462 has visible phenotype, non-suppressible by Rheb^{EP50.084-loxP}, Scer\GAL4^salm.EPv

(Ibar et al., 2013)

Scer\GAL4^salm.EPv, Tcs5^RNAi.UAS.462 has visible phenotype, non-suppressible by Myc^UAS.cZa, Scer\GAL4^salm.EPv

(Ibar et al., 2013)

Scer\GAL4^salm.EPv, Tcs5^RNAi.UAS.462 has visible phenotype, non-suppressible by Atg1^JF02273, Scer\GAL4^salm.EPv

(Ibar et al., 2013)

Scer\GAL4^salm.EPv, Tcs5^RNAi.UAS.462 has visible phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^salm.EPv

(Ibar et al., 2013)

Scer\GAL4^salm.EPv, Tcs5^RNAi.UAS.462 has visible phenotype, non-suppressible by Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^salm.EPv

(Ibar et al., 2013)

Scer\GAL4^salm.EPv, Tcs5^RNAi.UAS.462 has visible phenotype, non-suppressible by Akt^UAS.Exel, Scer\GAL4^salm.EPv

(Ibar et al., 2013)

Scer\GAL4^salm.EPv, Tcs5^RNAi.UAS.462 has visible phenotype, non-suppressible by Scer\GAL4^salm.EPv/gig^GD1454

(Ibar et al., 2013)

Suppressor of

Statement

Reference

Tcs5^RNAi.UAS.462, Scer\GAL4^Cg.PU is a suppressor of abnormal cell shape phenotype of Rac1^UAS.cLa, Scer\GAL4^Cg.PU

(Molina et al., 2022)

Other

Statement

Reference

Rab35^EYFP, Scer\GAL4^Hml.Δ, Tcs5^RNAi.UAS.462 has abnormal cell migration | P-stage phenotype

(Molina et al., 2022)

Rab35^S22N.UAS.YFP, Scer\GAL4^Cg.PU, Tcs5^RNAi.UAS.462 has abnormal cell shape phenotype

(Molina et al., 2022)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Cg.PU, Tcs5^RNAi.UAS.462 has hemocyte phenotype, suppressible by Mmus\Trp53rka^WT.UAS, Scer\GAL4^Cg.PU

(Molina et al., 2022)

Scer\GAL4^Cg.PU, Tcs5^RNAi.UAS.462 has hemocyte phenotype, suppressible | partially by Mmus\Trp53rka^KD.UAS, Scer\GAL4^Cg.PU

(Molina et al., 2022)

Scer\GAL4^Cg.PU, Tcs5^RNAi.UAS.462 has hemocyte phenotype, suppressible | partially by Rab35^UASp.YFP, Scer\GAL4^Cg.PU

(Molina et al., 2022)

Scer\GAL4^Cg.PU, Tcs5^RNAi.UAS.462 has hemocyte phenotype, suppressible | partially by Rac1^UAS.cLa, Scer\GAL4^Cg.PU

(Molina et al., 2022)

Scer\GAL4^Cg.PU, Tcs5^RNAi.UAS.462 has hemocyte phenotype, suppressible by Cdc42^V12.UAS, Scer\GAL4^Cg.PU

(Molina et al., 2022)

Scer\GAL4^salm.EPv, Tcs5^RNAi.UAS.462 has first posterior wing cell phenotype, suppressible by S6k^STDETE.UAS, Scer\GAL4^salm.EPv

(Ibar et al., 2013)

Scer\GAL4^Act5C.PU, Tcs5^RNAi.UAS.462 has wing disc | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by S6k^STDETE.UAS, Scer\GAL4^Act5C.PU

(Ibar et al., 2013)

Scer\GAL4^Act5C.PU, Tcs5^RNAi.UAS.462 has embryonic/larval fat body | somatic clone | third instar larval stage phenotype, suppressible | somatic clone by S6k^STDETE.UAS, Scer\GAL4^Act5C.PU

(Ibar et al., 2013)

NOT suppressed by

Statement

Reference

Scer\GAL4^salm.EPv, Tcs5^RNAi.UAS.462 has first posterior wing cell phenotype, non-suppressible by Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^salm.EPv

(Ibar et al., 2013)

Scer\GAL4^salm.EPv, Tcs5^RNAi.UAS.462 has first posterior wing cell phenotype, non-suppressible by Akt^UAS.Exel, Scer\GAL4^salm.EPv

(Ibar et al., 2013)

Scer\GAL4^salm.EPv, Tcs5^RNAi.UAS.462 has first posterior wing cell phenotype, non-suppressible by Scer\GAL4^salm.EPv/gig^GD1454

(Ibar et al., 2013)

Scer\GAL4^salm.EPv, Tcs5^RNAi.UAS.462 has first posterior wing cell phenotype, non-suppressible by Rheb^{EP50.084-loxP}, Scer\GAL4^salm.EPv

(Ibar et al., 2013)

Scer\GAL4^salm.EPv, Tcs5^RNAi.UAS.462 has first posterior wing cell phenotype, non-suppressible by Myc^UAS.cZa, Scer\GAL4^salm.EPv

(Ibar et al., 2013)

Scer\GAL4^salm.EPv, Tcs5^RNAi.UAS.462 has first posterior wing cell phenotype, non-suppressible by Atg1^JF02273, Scer\GAL4^salm.EPv

(Ibar et al., 2013)

Scer\GAL4^salm.EPv, Tcs5^RNAi.UAS.462 has first posterior wing cell phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^salm.EPv

(Ibar et al., 2013)

Suppressor of

Statement

Reference

Tcs5^RNAi.UAS.462, Scer\GAL4^Cg.PU is a suppressor of hemocyte phenotype of Rac1^UAS.cLa, Scer\GAL4^Cg.PU

(Molina et al., 2022)

Other

Statement

Reference

Rab35^EYFP, Scer\GAL4^Hml.Δ, Tcs5^RNAi.UAS.462 has hemocyte | P-stage phenotype

(Molina et al., 2022)

Rab35^S22N.UAS.YFP, Scer\GAL4^Cg.PU, Tcs5^RNAi.UAS.462 has hemocyte phenotype

(Molina et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

The reduction in the distance between wing veins 3 and 4 that is seen in animals expressing Prpk^{dsRNA.Scer\UAS.462} under the control of Scer\GAL4^salm.EPv is not suppressed if they are also co-expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX}, Akt1^{Scer\UAS.Exel}, gig^GD1454, Rheb^{EP50.084-loxP}, Atg1^JF02273 or dm^Scer\UAS.cZa.

The reduction in the distance between wing veins 3 and 4 and the reduction in cell size in the wing that is seen in animals expressing Prpk^{dsRNA.Scer\UAS.462} under the control of Scer\GAL4^salm.EPv is suppressed if they are also co-expressing S6k^{STDETE.Scer\UAS}.

Clones expressing Prpk^{dsRNA.Scer\UAS.462} and also co-expressing one of Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX}, Akt1^{Scer\UAS.Exel} or Rheb^{EP50.084-loxP} under the control of Scer\GAL4^Act5C.PU in the third larval instar wing disc and fat body are small and apoptotic, showing the same phenotype as clones expressing Prpk^{dsRNA.Scer\UAS.462} alone under the control of Scer\GAL4^Act5C.PU.

Co-expression of S6k^{STDETE.Scer\UAS} rescues the reduced size of clones in third larval instar wing disc and fat body expressing Prpk^{dsRNA.Scer\UAS.462} under the control of Scer\GAL4^Act5C.PU.

(Ibar et al., 2013)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa does not suppress the reduction in distance between wing veins 3 and 4 of caused by expression of Prpk^{dsRNA.Scer\UAS.462} under the control of Scer\GAL4^salm.EPv.

Co-expression of BacA\p35^Scer\UAS.cHa does not rescue the small size of clones in the third larval instar wing disc expressing Prpk^{dsRNA.Scer\UAS.462} under the control of Scer\GAL4^Act5C.PU (clones induced at 60 +/- 12 hours after egg laying), although the clones are no longer disaggregated.

(Ibar et al., 2013)

Complementation and Rescue Data

Rescued by

Tcs5^RNAi.UAS.462 is rescued by Scer\GAL4^salm.EPv/Tcs5^{UAS.C.Tag:MYC}

(Ibar et al., 2013)

Tcs5^RNAi.UAS.462 is rescued by Scer\GAL4^Act5C.PU/Tcs5^{UAS.C.Tag:MYC}

(Ibar et al., 2013)

Tcs5^RNAi.UAS.462 is rescued by Scer\GAL4^salm.EPv/Tcs5^{UAS.N.Tag:FLAG}

(Ibar et al., 2013)

Tcs5^RNAi.UAS.462 is rescued by Scer\GAL4^salm.EPv/Tcs5^{T221D.UAS.Tag:FLAG}

(Ibar et al., 2013)

Tcs5^RNAi.UAS.462 is rescued by Scer\GAL4^salm.EPv/Tcs5^{T221E.UAS.Tag:FLAG}

(Ibar et al., 2013)

Tcs5^RNAi.UAS.462 is rescued by Scer\GAL4^salm.EPv/Tcs5^{T221A.UAS.Tag:FLAG}

(Ibar et al., 2013)

Partially rescued by

Tcs5^RNAi.UAS.462 is partially rescued by Scer\GAL4^salm.EPv/Tcs5^{KD.UAS.Tag:V5}

(Ibar et al., 2013)

Comments

Co-expression of Prpk^{Scer\UAS.C.T:Hsap\MYC} rescues the reduction in larval size caused by expression of Prpk^{dsRNA.Scer\UAS.462} under the control of Scer\GAL4^da.G32.

Co-expression of Prpk^{Scer\UAS.C.T:Hsap\MYC} rescues the reduction in distance between wing veins 3 and 4 caused by expression of Prpk^{dsRNA.Scer\UAS.462} under the control of Scer\GAL4^salm.EPv.

Co-expression of Prpk^{Scer\UAS.C.T:Hsap\MYC} rescues the small and apoptotic nature of clones in the third larval instar wing disc expressing Prpk^{dsRNA.Scer\UAS.462} under the control of Scer\GAL4^Act5C.PU (clones induced at 60 +/- 12 hours after egg laying).

Co-expression of Prpk^{Scer\UAS.C.T:Hsap\MYC} rescues the small size of clones in the third larval instar fat body expressing Prpk^{dsRNA.Scer\UAS.462} under the control of Scer\GAL4^Act5C.PU (clones induced 36 +/- 12 hours after egg laying).

Co-expression of either Prpk^{T221D.Scer\UAS.T:Zzzz\FLAG}, Prpk^{T221E.Scer\UAS.T:Zzzz\FLAG} or Prpk^{T221A.Scer\UAS.T:Zzzz\FLAG} rescues the reduction in cell size seen in the wings of animals expressing Prpk^{dsRNA.Scer\UAS.462} under the control of Scer\GAL4^salm.EPv.

Co-expression of Prpk^{Scer\UAS.N.T:Zzzz\FLAG} rescues the reduction in cell size and the reduction in the distance between wing veins 3 and 4 that is seen in the wings of animals expressing Prpk^{dsRNA.Scer\UAS.462} under the control of Scer\GAL4^salm.EPv.

Co-expression of Prpk^{KD.Scer\UAS.T:SV5\V5} partially rescues the reduction in cell size and the reduction in the distance between wing veins 3 and 4 that is seen in the wings of animals expressing Prpk^{dsRNA.Scer\UAS.462} under the control of Scer\GAL4^salm.EPv.

(Ibar et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Prpk^{dsRNA.Scer\UAS.462}

Prpk^{dsRNA.UAS.462}

Tcs5^RNAi.UAS.462

Tcs5^{dsRNA.UAS.462}

Name Synonyms

Secondary FlyBase IDs

References (3)

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