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General Information
Symbol
Dmel\RhebEP50.084-loxP
Species
D. melanogaster
Name
FlyBase ID
FBal0159902
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Rheb
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The Scer\UAS sites at the 5' end of P{EP.Scer\UAS(loxP.Scer\UAS.y+).BN}RhebEP50.084 have been excised using P1\cre recombination to yield a single-headed EP element capable of driving Rheb expression.

Insertion components
P{EP.Scer\UAS.(-loxP).BN}RhebEP50.084-loxP
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Clones expressing RhebEP50.084-loxP under the control of Scer\GAL4Act5C.PU in the third larval instar wing disc and fat body are larger than control clones.

Ovarian follicle cells overexpressing RhebEP50.084-loxP (under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C) lack lysotracker staining even under starvation conditions, indicating that activation of IIS/TOR signaling is sufficient to suppress starvation-induced autophagy.

Cells in the fat body that are expressing RhebEP50.084-loxP show an increase in relative cell area compared to controls.

Overexpression of RhebEP50.084-loxP under the control of Scer\GAL4796 induces a significant increase in the synaptic domain.

Expression of RhebEP50.084-loxP under the control of Scer\GAL4GMR.PF results in a bulging eye phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT suppressed by
Suppressor of
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
NOT suppressed by
Suppressor of
Statement
Reference
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The reduction in the distance between wing veins 3 and 4 that is seen in animals expressing PrpkdsRNA.Scer\UAS.462 under the control of Scer\GAL4salm.EPv is not suppressed if they are also co-expressing RhebEP50.084-loxP.

Clones expressing PrpkdsRNA.Scer\UAS.462 and also co-expressing RhebEP50.084-loxP under the control of Scer\GAL4Act5C.PU in the third larval instar wing disc and fat body are small and apoptotic, showing the same phenotype as clones expressing PrpkdsRNA.Scer\UAS.462 alone under the control of Scer\GAL4Act5C.PU.

The increase in cell area relative to control cells that is seen in cells in the fat body that are expressing RhebEP50.084-loxP is enhanced if the animals are also homozygous for RagCEY11726.

The variable reduction in cell size and induction of autophagy in the fat body that is seen in animals expressing Atg1GS10797 under the control of Scer\GAL4Cg.PA is suppressed if the animals are also co-expressing RhebEP50.084-loxP.

The bulging eye phenotype caused by expression of RhebEP50.084-loxP under the control of Scer\GAL4GMR.PF is not suppressed by simultaneous coexpression of both scylEP22.1 and chrbEP1035.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
RhebEP50.084-loxP
Name Synonyms
Secondary FlyBase IDs
    References (11)