FlyBase Allele Report: Dmel\Pyk[GD16178]

General Information

Symbol

Dmel\Pyk^GD16178

Species

D. melanogaster

Name

FlyBase ID

FBal0208189

Feature type

allele

Associated gene

Dmel\Pyk

Associated Insertion(s)

Carried in Construct

P{GD16178}

Also Known As

UAS-Pyk^RNAi, PK-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD16178}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD16178

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of disease of metabolism

CEA

(Devilliers et al., 2021)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | nutrition conditional, with Scer\GAL4^Cg.PA

(Garrido et al., 2015)

decreased cell size | somatic clone | nutrition conditional, with Scer\GAL4^Act5C.PP

(Garrido et al., 2015)

decreased size | adult stage, with Scer\GAL4^C-765

(Spannl et al., 2020)

lethal, with Scer\GAL4^btl.PS

(Hudson et al., 2023)

lethal | nutrition conditional, with Scer\GAL4^Cg.PA

(Garrido et al., 2015)

lethal - all die before end of second instar larval stage, with Scer\GAL4^da.PU

(Devilliers et al., 2021)

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

short lived, with Scer\GAL4^da.PU

(Devilliers et al., 2021)

short lived | RU486 conditional, with Scer\GAL4^{repo.Switch.2301}

(Martin et al., 2021)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

embryonic myoblast, with Scer\GAL4^kirre.PU

(Tixier et al., 2013)

larval somatic muscle cell, with Scer\GAL4^kirre.PU

(Tixier et al., 2013)

wing, with Scer\GAL4^C-765

(Spannl et al., 2020)

Detailed Description

Statement

Reference

The expression of PyK^GD16178 from the onset of adulthood, under the combined control of Scer\GAL4^da.PU and Gal80[ts], leads to decreased lifespan.

(Devilliers et al., 2021)

The expression of PyK^GD16178 under the control of Scer\GAL4^{repo.Switch.2301} from day 1 of adulthood (induced by feeding of RU486) leads to decreased lifespan.

(Martin et al., 2021)

When fed on a low carbohydrate diet (0% sucrose supplement), no developmental delay is observed for Scer\GAL4^Cg.PA PyK^GD16178 (plus Dcr-2^Scer\UAS.cDa) animals. In contrast, when fed a 10% or 20% sucrose supplemented diet, these animals exhibit a significant delay or most die as larvae, respectively.

The size of cells in flip-out Scer\GAL4^Act5C.PP PyK^GD16178 (plus Dcr-2^Scer\UAS.cDa) clones is almost normal in larvae fed a low carbohydrate diet, but is drastically reduced in larvae fed a 20% sucrose supplemented diet.

(Garrido et al., 2015)

Embryos expressing PyK^GD16178 under the control of Scer\GAL4^kirre.PU have thinner muscles than normal and unfused myoblasts are present.

(Tixier et al., 2013)

Expression under the control of Scer\GAL4^Mef2.PR results in early larval lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

16178

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^da.PU/Pyk^GD16178 is a suppressor | partially of increased cell size | third instar larval stage phenotype of Pten¹¹⁷

(Devilliers et al., 2021)

NOT Suppressor of

Statement

Reference

Scer\GAL4^da.PU/Pyk^GD16178 is a non-suppressor of increased cell size | third instar larval stage phenotype of Rheb^{EP50.084-loxP}, Scer\GAL4^Tub.PU

(Devilliers et al., 2021)

Pyk^GD16178, Scer\GAL4^Cg.PA is a non-suppressor of increased body size | pupal stage phenotype of Myc^UAS.Tag:HA, Scer\GAL4^Cg.PA

(Parisi et al., 2013)

Other

Statement

Reference

Df(2L)FASN^Δ24-23, Pyk^GD16178, Scer\GAL4^Act5C.PP has decreased cell size | somatic clone | nutrition conditional phenotype

(Garrido et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

Pyk^GD16178, Scer\GAL4^drm.7.1 is an enhancer of terminal tracheal cell | larval stage phenotype of Scer\GAL4^drm.7.1, trc^GL01127

(Hudson et al., 2023)

Suppressor of

Statement

Reference

Scer\GAL4^da.PU/Pyk^GD16178 is a suppressor | partially of larval fat cell | somatic clone | third instar larval stage phenotype of Pten¹¹⁷

(Devilliers et al., 2021)

NOT Suppressor of

Statement

Reference

Scer\GAL4^da.PU/Pyk^GD16178 is a non-suppressor of larval fat cell | somatic clone | third instar larval stage phenotype of Rheb^{EP50.084-loxP}, Scer\GAL4^Tub.PU

(Devilliers et al., 2021)

Pyk^GD16178, Scer\GAL4^Cg.PA is a non-suppressor of wing phenotype of Myc^UAS.Tag:HA, Scer\GAL4^Cg.PA

(Parisi et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The size of cells in Df(2L)FASN^Δ24-23 MARCM clones expressing PyK^GD16178 (plus Dcr-2^Scer\UAS.cDa) is hardly reduced in larvae fed a low carbohydrate diet. However, in larvae fed a 20% sucrose supplemented diet, the size of these cells is dramatically reduced.

(Garrido et al., 2015)

Co-expression of PyK^GD16178 does not prevent the Scer\GAL4^Cg.PA-mediated expression of Myc^{Scer\UAS.T:Ivir\HA1} resulting in abnormally large pupae and large wings.

(Parisi et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v49533

w¹¹¹⁸; P{GD16178}v49533

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

16178

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

PyK^GD16178

Pyk^GD16178

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

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