FlyBase Allele Report: Dmel\RagC-D[EY11726]

General Information

Symbol

Dmel\RagC-D^EY11726

Species

D. melanogaster

Name

FlyBase ID

FBal0160899

Feature type

allele

Associated gene

Dmel\RagC-D

Associated Insertion(s)

P{EPgy2}RagC-D^EY11726

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{EPgy2}RagC-D^EY11726

Cytology

Description

Insertion in the 5' UTR.

(Kim et al., 2008)

Allele components

Component

Use(s)

Inserted element

P{EPgy2}

misexpression element

Regulatory region(s)

UASp

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:8,163,407..8,163,407 [+]

(Zhang et al., 2015, Kim et al., 2008, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage

(Wong et al., 2015)

lethal - all die before end of larval stage

(Kim et al., 2008)

some die during third instar larval stage

(Kim et al., 2008)

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage

(Wong et al., 2015)

NMJ bouton | third instar larval stage

(Wong et al., 2015)

Detailed Description

Statement

Reference

RagC-D^EY11726/RagC-D^EY11726 third instar larvae show fewer neuromuscular junction boutons compared to RagC-D^EY11726/+ controls.

Raising RagC-D^EY11726/RagC-D^EY11726 larvae on a protein-rich diet and/or supplementing their food with ALK inhibitor CH5424802 does not rescue the synaptic growth defect and the number of neuromuscular junction boutons remains low compared to wild-type.

(Wong et al., 2015)

Under normal fed conditions, RagC^EY11726 clones in the larval fat body show in a potent decrease in cell size compared with neighbouring control cells. Under starvation conditions, RagC^EY11726 clones in the larval fat body have no effect on cell size compared to controls.

(Kim et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

RagC-D^EY11726/RagC-D^EY11726 is an enhancer of increased cell size phenotype of Rheb^{EP50.084-loxP}

(Kim et al., 2008)

Suppressor of

Statement

Reference

RagC-D^EY11726, Scer\GAL4^dpp.blk1 is a suppressor | partially of visible | adult stage phenotype of Mitf^UAS.cZa, Scer\GAL4^dpp.blk1

(Zhang et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

RagC-D^EY11726, Scer\GAL4^dpp.blk1 is a suppressor | partially of wing | adult stage phenotype of Mitf^UAS.cZa, Scer\GAL4^dpp.blk1

(Zhang et al., 2015)

Other

Statement

Reference

RagC-D^EY11726, bsk¹/bsk[+] has neuromuscular junction | third instar larval stage phenotype

(Wong et al., 2015)

RagC-D^EY11726, bsk¹/bsk[+] has NMJ bouton | third instar larval stage phenotype

(Wong et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

RagC-D^EY11726/+,bsk¹/+ double heterozygotes show decreased number of NMJ boutons in third instar larvae compared to either RagC-D^EY11726/+ or bsk¹/+ single heterozygotes.

(Wong et al., 2015)

The small underdeveloped wing characteristic for adult flies expressing Mitf^Scer\UAS.cZa under the control of Scer\GAL4^dpp.blk1 is significantly suppressed by co-expression of RagC-D^EY11726.

(Zhang et al., 2015)

The increase in cell area relative to control cells that is seen in cells in the fat body that are expressing Rheb^{EP50.084-loxP} is enhanced if the animals are also homozygous for RagC^EY11726.

(Kim et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

21364

y¹ w^67c23; P{EPgy2}RagC-D^EY11726/CyO

Notes on Origin

Discoverer

Comments

Excision of the insertion can restore viability.

(Kim et al., 2008)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG8707^EY11726

RagC-D^EY11726

RagC^EY11726

(Kim et al., 2017)

dRagC^EY11726

(Kim et al., 2008)

ragC^Δ

(Wong et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

Open Close