FlyBase Allele Report: Dmel\Tl[10b.UAS.cUa]

General Information

Symbol

Dmel\Tl^10b.UAS.cUa

Species

D. melanogaster

Name

FlyBase ID

FBal0294192

Feature type

allele

Associated gene

Dmel\Tl

Associated Insertion(s)

Carried in Construct

P{UAS-Tl.10b.U}

Also Known As

UAS-Toll^10b, UAS-Tl^10b

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(FlyBase Curators, 2025-)

Progenitor genotype

Carried in construct

P{UAS-Tl.10b.U}

Cytology

Description

UAS regulatory sequences drive expression of a constitutively active form of Tl.

(FlyBase Curators, 2025-)

FlyBase curator comment: this entry represents a transgenic construct where the particular construct used cannot be determined.

(FlyBase Curators, 2025-)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of glucose metabolism disease

CEA

(Roth et al., 2018)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of glucose metabolism disease

is ameliorated by Akt^{T342D.UAS.Tag:HA}

(Roth et al., 2018)

is ameliorated by Akt^{UAS.Tag:Myr(Unk)}

(Roth et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell growth | cell autonomous | larval stage | somatic clone, with Scer\GAL4^Act5C.PP

(Roth et al., 2018)

abnormal developmental rate, with Scer\GAL4^r4

(Diangelo et al., 2009)

abnormal size | cell autonomous | larval stage | somatic clone, with Scer\GAL4^Act5C.PP

(Roth et al., 2018)

decreased body size | adult stage, with Scer\GAL4^r4

(Diangelo et al., 2009)

decreased body size | larval stage, with Scer\GAL4^r4

(Diangelo et al., 2009)

decreased body size | pupal stage, with Scer\GAL4^r4

(Roth et al., 2018)

decreased cell size | cell autonomous | larval stage | somatic clone, with Scer\GAL4^Act5C.PP

(Roth et al., 2018)

majority die during P-stage, with Scer\GAL4^r4

(Roth et al., 2018)

partially lethal, with Scer\GAL4^r4

(Diangelo et al., 2009)

partially lethal - majority die, with Scer\GAL4^r4

(Roth et al., 2018)

Phenotype Manifest In

fat cell | cell autonomous | larval stage | somatic clone, with Scer\GAL4^Act5C.PP

(Roth et al., 2018)

nucleus | cell autonomous | larval stage | somatic clone, with Scer\GAL4^Act5C.PP

(Roth et al., 2018)

pupa, with Scer\GAL4^r4

(Roth et al., 2018)

Detailed Description

Statement

Reference

Clonal expression of Tl^10b.UAS.cUa under the control of Scer\GAL4^Act5C.PP in the larval fat body induces smaller cells with smaller nuclei, as compared to neighboring control cells.

The expression of Tl^10b.UAS.cUa under the control of Scer\GAL4^r4 leads to considerable smaller pupae that have severely lower weight, as compared to controls.

(Roth et al., 2018)

Third instar larvae expressing Tl^{10b.Scer\UAS.cUa} under the control of Scer\GAL4^r4 have a significantly reduced triglyceride/protein ratio compared to controls.

Larvae expressing Tl^{10b.Scer\UAS.cUa} under the control of Scer\GAL4^r4 are significantly reduced in size compared to controls. They show a developmental delay of approximately 24-36 hours and have reduced viability. Adult escapers are small than wild type.

(Diangelo et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^r4, Tl^10b.UAS.cUa has increased mortality during development phenotype, non-enhanceable by Akt^UAS.Tag:HA, Scer\GAL4^r4

(Roth et al., 2018)

Scer\GAL4^r4, Tl^10b.UAS.cUa has increased mortality during development phenotype, non-enhanceable by Akt^{T342A.UAS.Tag:HA}, Scer\GAL4^r4

(Roth et al., 2018)

Scer\GAL4^r4, Tl^10b.UAS.cUa has increased mortality during development phenotype, non-enhanceable by Akt^{S505A.UAS.Tag:HA}, Scer\GAL4^r4

(Roth et al., 2018)

Scer\GAL4^r4, Tl^10b.UAS.cUa has increased mortality during development phenotype, non-enhanceable by Akt^{S505D.UAS.Tag:HA}, Scer\GAL4^r4

(Roth et al., 2018)

Suppressed by

Statement

Reference

Scer\GAL4^Act5C.PP, Tl^10b.UAS.cUa has abnormal size | somatic clone | cell autonomous | larval stage phenotype, suppressible | somatic clone by InR^A1325D.UAS, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Scer\GAL4^Act5C.PP, Tl^10b.UAS.cUa has decreased cell size | somatic clone | cell autonomous | larval stage phenotype, suppressible | somatic clone by Akt^{UAS.Tag:Myr(Unk)}, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Scer\GAL4^Act5C.PP, Tl^10b.UAS.cUa has abnormal cell growth | somatic clone | cell autonomous | larval stage phenotype, suppressible | somatic clone by Akt^{UAS.Tag:Myr(Unk)}, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Scer\GAL4^Act5C.PP, Tl^10b.UAS.cUa has abnormal size | somatic clone | cell autonomous | larval stage phenotype, suppressible | somatic clone by Akt^{UAS.Tag:Myr(Unk)}, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Scer\GAL4^Act5C.PP, Tl^10b.UAS.cUa has decreased cell size | somatic clone | cell autonomous | larval stage phenotype, suppressible | partially | somatic clone by Akt^{T342D.UAS.Tag:HA}, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Scer\GAL4^Act5C.PP, Tl^10b.UAS.cUa has abnormal cell growth | somatic clone | cell autonomous | larval stage phenotype, suppressible | partially | somatic clone by Akt^{T342D.UAS.Tag:HA}, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Scer\GAL4^r4, Tl^10b.UAS.cUa has decreased body size | pupal stage phenotype, suppressible by Akt^{T342D.UAS.Tag:HA}, Scer\GAL4^r4

(Roth et al., 2018)

Scer\GAL4^r4, Tl^10b.UAS.cUa has increased mortality during development phenotype, suppressible by Dif^HM05257, Scer\GAL4^r4

(Roth et al., 2018)

Scer\GAL4^r4, Tl^10b.UAS.cUa has increased mortality during development phenotype, suppressible by Akt^{T342D.UAS.Tag:HA}, Scer\GAL4^r4

(Roth et al., 2018)

Scer\GAL4^r4, Tl^10b.UAS.cUa has some die during P-stage phenotype, suppressible by Akt^{T342D.UAS.Tag:HA}, Scer\GAL4^r4

(Roth et al., 2018)

Scer\GAL4^r4, Tl^10b.UAS.cUa has decreased body size | larval stage phenotype, suppressible by Akt^{UAS.Tag:Myr(Unk)}, Scer\GAL4^r4

(Diangelo et al., 2009)

Scer\GAL4^r4, Tl^10b.UAS.cUa has decreased body size | adult stage phenotype, suppressible by Akt^{UAS.Tag:Myr(Unk)}, Scer\GAL4^r4

(Diangelo et al., 2009)

NOT suppressed by

Statement

Reference

Scer\GAL4^Act5C.PP, Tl^10b.UAS.cUa has decreased cell size | somatic clone | cell autonomous | larval stage phenotype, non-suppressible | somatic clone by InR^A1325D.UAS, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Scer\GAL4^Act5C.PP, Tl^10b.UAS.cUa has abnormal cell growth | somatic clone | cell autonomous | larval stage phenotype, non-suppressible | somatic clone by InR^A1325D.UAS, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Scer\GAL4^r4, Tl^10b.UAS.cUa has increased mortality during development phenotype, non-suppressible by Akt^{S505A.UAS.Tag:HA}, Scer\GAL4^r4

(Roth et al., 2018)

Scer\GAL4^r4, Tl^10b.UAS.cUa has increased mortality during development phenotype, non-suppressible by Akt^{S505D.UAS.Tag:HA}, Scer\GAL4^r4

(Roth et al., 2018)

Scer\GAL4^Act5C.PP, Tl^10b.UAS.cUa has decreased cell size | somatic clone | cell autonomous | larval stage phenotype, non-suppressible by Pdk1^UAS.Tag:MYC, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Scer\GAL4^Act5C.PP, Tl^10b.UAS.cUa has abnormal cell growth | somatic clone | cell autonomous | larval stage phenotype, non-suppressible by Pdk1^UAS.Tag:MYC, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Scer\GAL4^Act5C.PP, Tl^10b.UAS.cUa has abnormal size | somatic clone | cell autonomous | larval stage phenotype, non-suppressible | somatic clone by Pdk1^UAS.Tag:MYC, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Scer\GAL4^r4, Tl^10b.UAS.cUa has decreased body size | pupal stage phenotype, non-suppressible by Pdk1^UAS.Tag:MYC, Scer\GAL4^r4

(Roth et al., 2018)

Scer\GAL4^r4, Tl^10b.UAS.cUa has increased mortality during development phenotype, non-suppressible by Akt^UAS.Tag:HA, Scer\GAL4^r4

(Roth et al., 2018)

Scer\GAL4^r4, Tl^10b.UAS.cUa has increased mortality during development phenotype, non-suppressible by Akt^{T342A.UAS.Tag:HA}, Scer\GAL4^r4

(Roth et al., 2018)

Suppressor of

Statement

Reference

Tl^10b.UAS.cUa, Scer\GAL4^Act5C.PP is a suppressor | somatic clone of abnormal size | somatic clone | cell autonomous | larval stage phenotype of InR^A1325D.UAS, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Tl^10b.UAS.cUa, Scer\GAL4^Act5C.PP is a suppressor | partially | somatic clone of abnormal size | somatic clone | cell autonomous | larval stage phenotype of Akt^{UAS.Tag:Myr(Unk)}, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Tl^10b.UAS.cUa, Scer\GAL4^Act5C.PP is a suppressor | somatic clone of increased cell size | somatic clone | cell autonomous | larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Tl^10b.UAS.cUa, Scer\GAL4^Act5C.PP is a suppressor | somatic clone of abnormal cell growth | somatic clone | cell autonomous | larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Tl^10b.UAS.cUa, Scer\GAL4^Act5C.PP is a suppressor | somatic clone of abnormal size | somatic clone | cell autonomous | larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Tl^10b.UAS.cUa, Scer\GAL4^Act5C.PP is a suppressor | somatic clone of increased cell size | somatic clone | cell autonomous | larval stage phenotype of Pdk1^UAS.Tag:MYC, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Tl^10b.UAS.cUa, Scer\GAL4^Act5C.PP is a suppressor | somatic clone of abnormal cell growth | somatic clone | cell autonomous | larval stage phenotype of Pdk1^UAS.Tag:MYC, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Tl^10b.UAS.cUa, Scer\GAL4^Act5C.PP is a suppressor | somatic clone of abnormal size | somatic clone | cell autonomous | larval stage phenotype of Pdk1^UAS.Tag:MYC, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Other

Statement

Reference

Tl^10b.UAS.cUa, rictor^Δ2 has decreased body size | pupal stage phenotype

(Roth et al., 2018)

InR^A1325D.UAS, Scer\GAL4^Act5C.PP, Tl^10b.UAS.cUa has decreased cell size | somatic clone | cell autonomous | larval stage phenotype

(Roth et al., 2018)

InR^A1325D.UAS, Scer\GAL4^Act5C.PP, Tl^10b.UAS.cUa has abnormal cell growth | somatic clone | cell autonomous | larval stage phenotype

(Roth et al., 2018)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Act5C.PP, Tl^10b.UAS.cUa has nucleus | somatic clone | cell autonomous | larval stage phenotype, suppressible | somatic clone by InR^A1325D.UAS, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

(Roth et al., 2018)

Scer\GAL4^Act5C.PP, Tl^10b.UAS.cUa has nucleus | somatic clone | cell autonomous | larval stage phenotype, suppressible | somatic clone by Akt^{UAS.Tag:Myr(Unk)}, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Scer\GAL4^Act5C.PP, Tl^10b.UAS.cUa has fat cell | somatic clone | cell autonomous | larval stage phenotype, suppressible | somatic clone by Akt^{UAS.Tag:Myr(Unk)}, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Scer\GAL4^Act5C.PP, Tl^10b.UAS.cUa has fat cell | somatic clone | cell autonomous | larval stage phenotype, suppressible | partially | somatic clone by Akt^{T342D.UAS.Tag:HA}, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Scer\GAL4^r4, Tl^10b.UAS.cUa has pupa phenotype, suppressible by Akt^{T342D.UAS.Tag:HA}, Scer\GAL4^r4

(Roth et al., 2018)

NOT suppressed by

Statement

Reference

Scer\GAL4^r4, Tl^10b.UAS.cUa has pupa phenotype, non-suppressible by Pdk1^UAS.Tag:MYC, Scer\GAL4^r4

(Roth et al., 2018)

Scer\GAL4^Act5C.PP, Tl^10b.UAS.cUa has nucleus | somatic clone | cell autonomous | larval stage phenotype, non-suppressible | somatic clone by Pdk1^UAS.Tag:MYC, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Scer\GAL4^Act5C.PP, Tl^10b.UAS.cUa has fat cell | somatic clone | cell autonomous | larval stage phenotype, non-suppressible | somatic clone by Pdk1^UAS.Tag:MYC, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Suppressor of

Statement

Reference

Tl^10b.UAS.cUa, Scer\GAL4^Act5C.PP is a suppressor | somatic clone of nucleus | somatic clone | cell autonomous | larval stage phenotype of InR^A1325D.UAS, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Tl^10b.UAS.cUa, Scer\GAL4^Act5C.PP is a suppressor | somatic clone of fat cell | somatic clone | cell autonomous | larval stage phenotype of InR^A1325D.UAS, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Tl^10b.UAS.cUa, Scer\GAL4^Act5C.PP is a suppressor | partially | somatic clone of nucleus | somatic clone | cell autonomous | larval stage phenotype of Akt^{UAS.Tag:Myr(Unk)}, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Tl^10b.UAS.cUa, Scer\GAL4^Act5C.PP is a suppressor | partially | somatic clone of fat cell | somatic clone | cell autonomous | larval stage phenotype of Akt^{UAS.Tag:Myr(Unk)}, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Tl^10b.UAS.cUa, Scer\GAL4^Act5C.PP is a suppressor | somatic clone of nucleus | somatic clone | cell autonomous | larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Tl^10b.UAS.cUa, Scer\GAL4^Act5C.PP is a suppressor | somatic clone of fat cell | somatic clone | cell autonomous | larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Tl^10b.UAS.cUa, Scer\GAL4^Act5C.PP is a suppressor | somatic clone of nucleus | somatic clone | cell autonomous | larval stage phenotype of Pdk1^UAS.Tag:MYC, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Tl^10b.UAS.cUa, Scer\GAL4^Act5C.PP is a suppressor | somatic clone of fat cell | somatic clone | cell autonomous | larval stage phenotype of Pdk1^UAS.Tag:MYC, Scer\GAL4^Act5C.PP

(Roth et al., 2018)

Other

Statement

Reference

Tl^10b.UAS.cUa, rictor^Δ2 has pupa phenotype

(Roth et al., 2018)

Pdk1³³, Scer\GAL4^r4, Tl^10b.UAS.cUa has pupa phenotype

(Roth et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The expression of Tl^10b.UAS.cUa under the control of Scer\GAL4^r4 leads to smaller pupae with lower weight, whereas clonal expression under the control of Scer\GAL4^Act5C.PP in the larval fat body induces induces smaller cells; both cases are substantially suppressed by the co-expression of Akt1^{T342D.UAS.Tag:HA}. However, the smaller size and decreased weight of pupae expressing Tl^10b.UAS.cUa under the control of Scer\GAL4^r4 is still observed upon co-expression of Pdk1^UAS.Tag:MYC.

Larval fat body clones co-expressing Tl^10b.UAS.cUa and Akt1^{UAS.Tag:Myr(Unk)} under the control of Scer\GAL4^Act5C.PP exhibit the same cell and nucleus size increases as observed upon Akt1^{UAS.Tag:Myr(Unk)} single expression (although the increased nucleus size is partially suppressed), instead of the cell and nucleus size decreases observed upon Tl^10b.UAS.cUa single expression. Conversely larval fat body clones co-expressing Tl^10b.UAS.cUa and Pdk1^UAS.Tag:MYC under the control of Scer\GAL4^Act5C.PP exhibit the same cell and nucleus size decreases as observed upon Tl^10b.UAS.cUa single expression, instead of the cell and nucleus size increases observed upon Pdk1^UAS.Tag:MYC single expression. Larval fat body clones co-expressing Tl^10b.UAS.cUa and InR^A1325D.UAS under the control of Scer\GAL4^Act5C.PP, however, do not show exhibit the nucleus sizes increases and decreases respectively observed upon single expressions of Akt1^{UAS.Tag:Myr(Unk)} and Tl^10b.UAS.cUa.

Clonal expression of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)} under the control of Scer\GAL4^Act5C.PP in the larval fat body induces extremely large cells with extremely large nuclei, both of which are suppressed by clonal co-expression of Tl^10b.UAS.cUa.

rictor^Δ2-homozygous or heterozygous pupae show a significant decrease in weight upon the expression of Tl^10b.UAS.cUa under the control of Scer\GAL4^r4; there is also apparently smaller pupae only in the rictor^Δ2-homozygous background.

Pdk1³³ heterozygous, but not of homozygous, pupae show a signifcan decrease in weight upon expression of Tl^10b.UAS.cUa under the control of Scer\GAL4^r4.

(Roth et al., 2018)

Co-expression of Akt1^{Scer\UAS.T:Uuuu\Myr5} rescues the reduced size of larvae and adult escapers expressing Tl^{10b.Scer\UAS.cUa} under the control of Scer\GAL4^r4.

(Diangelo et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Tl^{10b.Scer\UAS.cUa}

Tl^10b.UAS.cUa

Name Synonyms

Secondary FlyBase IDs

References (10)

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