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General Information
Symbol
Dmel\Pdk133
Species
D. melanogaster
Name
FlyBase ID
FBal0265719
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the progenitor insertion. Approximately 600bp of the original insertion remain.

Insertion components
P{?SUPor-P}Pdk133
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Pdk133 homozygous pupae are considerable smaller and show a severely lower weight severely, as compared to heterozygous controls.

Homozygotes survive to the third instar larval stage and to the adult stage in reduced numbers. Homozygous adults show a severe reduction in body size compared to controls.

Motoneuron cell bodies are smaller in size than normal in homozygous larvae.

Absolute bouton number at the neuromuscular junction is normal in homozygous third instar larvae, while there is a 35% decrease in bouton size. The muscle fibres at the neuromuscular junction are 35% smaller than normal. As a result, normalised bouton number/muscle area at the mutant neuromuscular junction is increased by 55% compared to wild type. The number of active zones and the total synaptic area is significantly reduced at the mutant neuromuscular junction.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
NOT suppressed by
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

Expressing Tl10b.UAS.cUa under the control of Scer\GAL4r4 significantly decreases the weight of Pdk133 heterozygotes, but not of homozygotes.

Expression of S6kSTDETE.Scer\UAS under the control of Scer\GAL4VGlut-OK371 partially rescues the reduction in bouton size at the neuromuscular junction which is seen in Pdk133 larvae. In addition, the number of boutons at the neuromuscular junction is increased compared to wild type.

Expression of S6kScer\UAS.cCa under the control of Scer\GAL4VGlut-OK371 does not rescue the reduction in bouton size at the neuromuscular junction which is seen in Pdk133 larvae.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

Expression of Pdk1Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4VGlut-OK371 fully restores the defects in bouton size which are seen at the Pdk133 neuromuscular junction, but the defects in muscle size that are also seen at the Pdk133 neuromuscular junction are not rescued.

Expression of Pdk1Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4C57 fully restores the defects in muscle size which are seen at the Pdk133 neuromuscular junction, but the defects in bouton size that are also seen at the Pdk133 neuromuscular junction are not rescued.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Pdk133
Name Synonyms
Secondary FlyBase IDs
    References (3)