FlyBase Allele Report: Zzzz\poly-GA[400.UAS]

General Information

Symbol

Zzzz\poly-GA^400.UAS

Species

a. artificial

Name

FlyBase ID

FBal0393033

Feature type

allele

Associated gene

Zzzz\poly-GA

Associated Insertion(s)

Carried in Construct

P{UAS-GA400}

Transgenic product class

short_tandem_repeat_expansion

(Morón-Oset et al., 2023)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

short_tandem_repeat_expansion

(Morón-Oset et al., 2023)

Mutagen

in vitro construct

(Morón-Oset et al., 2023)

Progenitor genotype

Carried in construct

P{UAS-GA400}

Cytology

Description

UASt regulates expression of 400 Glycine-Alanine ("GA") dipeptide repeats, representing a translation product of the expanded GGGGCC hexanucleotide repeat from Hsap\C9orf72 that causes Frontotemporal Dementia (FTD) and Amyotrophic Lateral Sclerosis (ALS). The repeats are interrupted in two places restriction sites used in the cloning strategy (order of repeats is GA100 - SmaI site - GA200 - XbaI site - GA100).

(Morón-Oset et al., 2023)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Zzzz\poly-GA

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of C9orf72 frontotemporal dementia and/or amyotrophic lateral sclerosis

CEA

(Morón-Oset et al., 2023)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of C9orf72 frontotemporal dementia and/or amyotrophic lateral sclerosis

is ameliorated by InR^A1325D.UAS

(Morón-Oset et al., 2023)

is ameliorated by Tango1^HMS05801

(Morón-Oset et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

This allele represents a human variant implicated in disease.

(Morón-Oset et al., 2023)

C9orf72:n.intron1_p.[400GlyAla]

(FlyBase curators, 2019-)

Variants Synonym(s)

C9ORF72, (GGGGCC)n REPEAT EXPANSION

Associated human disease model(s)

frontotemporal dementia and/or amyotrophic lateral sclerosis 1

External database links

ClinVar: C9orf72_31151

Comments concerning this variant

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult brain | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Morón-Oset et al., 2023)

eye, with Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has visible | adult stage phenotype, enhanceable by ref(2)P^HMS00551/Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has decreased size | adult stage phenotype, enhanceable by ref(2)P^HMS00551/Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has visible | adult stage phenotype, enhanceable by ref(2)P^HMS00938/Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has decreased size | adult stage phenotype, enhanceable by ref(2)P^HMS00938/Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has visible | adult stage phenotype, enhanceable by Hsap\UNC119^UAS.Tag:HA, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has decreased size | adult stage phenotype, enhanceable by Hsap\UNC119^UAS.Tag:HA, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has short lived | RU486 conditional phenotype, enhanceable by Scer\GAL4^{elav.Switch.PO}/ref(2)P^HMS00551

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has short lived | RU486 conditional phenotype, enhanceable by Scer\GAL4^{elav.Switch.PO}/ref(2)P^HMS00938

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has short lived | RU486 conditional phenotype, enhanceable by Atg1^GS10797, Scer\GAL4^{elav.Switch.PO}

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has visible | adult stage phenotype, enhanceable by Hsp22^GLC01686, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has short lived | RU486 conditional phenotype, enhanceable by Scer\GAL4^{elav.Switch.PO}/unc-119^GD9180

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has short lived | RU486 conditional phenotype, enhanceable by Hsap\UNC119^UAS.Tag:HA, Scer\GAL4^{elav.Switch.PO}

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has decreased size | adult stage phenotype, enhanceable by Hsp22^GLC01686, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has visible | adult stage phenotype, enhanceable by Hsp83^HMS00899, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has decreased size | adult stage phenotype, enhanceable by Hsp83^HMS00899, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has visible | adult stage phenotype, non-enhanceable by PEK^e01744

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has visible | adult stage phenotype, non-enhanceable by Atg5^RNAi.UAS.cUa, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has decreased size | adult stage phenotype, non-enhanceable by Atg5^RNAi.UAS.cUa, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has visible | adult stage phenotype, non-enhanceable by Rpn11^UAS.cMa, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has decreased size | adult stage phenotype, non-enhanceable by PEK^e01744

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has decreased size | adult stage phenotype, non-enhanceable by Rpn11^UAS.cMa, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has visible | adult stage phenotype, non-enhanceable by Rpn6^UAS.cTa, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has decreased size | adult stage phenotype, non-enhanceable by Rpn6^UAS.cTa, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has visible | adult stage phenotype, non-enhanceable by unc-119^GD9180/Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has decreased size | adult stage phenotype, non-enhanceable by unc-119^GD9180/Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has short lived | RU486 conditional phenotype, non-enhanceable by PEK^e01744

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has short lived | RU486 conditional phenotype, non-enhanceable by Hsp22^GLC01686, Scer\GAL4^{elav.Switch.PO}

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has short lived | RU486 conditional phenotype, non-enhanceable by Hsp83^HMS00899, Scer\GAL4^{elav.Switch.PO}

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has short lived | RU486 conditional phenotype, non-enhanceable by Scer\GAL4^{elav.Switch.PO}/ref(2)P^UAS.Tag:HA

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has short lived | RU486 conditional phenotype, non-enhanceable by Atg5^RNAi.UAS.cUa, Scer\GAL4^{elav.Switch.PO}

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has short lived | RU486 conditional phenotype, non-enhanceable by Rpn11^UAS.cMa/Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has short lived | RU486 conditional phenotype, non-enhanceable by Rpn6^UAS.cTa, Scer\GAL4^{elav.Switch.PO}

(Morón-Oset et al., 2023)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has visible | adult stage phenotype, suppressible | partially by ref(2)P^UAS.Tag:HA/Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has decreased size | adult stage phenotype, suppressible | partially by ref(2)P^UAS.Tag:HA/Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has visible | adult stage phenotype, suppressible | partially by Atg1^GS10797, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has decreased size | adult stage phenotype, suppressible | partially by Atg1^GS10797, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has visible | adult stage phenotype, suppressible | partially by InR^A1325D.UAS, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has decreased size | adult stage phenotype, suppressible by InR^A1325D.UAS, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has decreased rate of adult locomotory behavior | RU486 conditional phenotype, suppressible | partially by InR^A1325D.UAS, Scer\GAL4^{elav.Switch.PO}

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has short lived | RU486 conditional phenotype, suppressible | partially by InR^A1325D.UAS, Scer\GAL4^{elav.Switch.PO}

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has decreased rate of adult locomotory behavior | RU486 conditional phenotype, suppressible by Scer\GAL4^{elav.Switch.PO}/Tango1^HMS05801

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has short lived | RU486 conditional phenotype, suppressible | partially by Scer\GAL4^{elav.Switch.PO}/Tango1^HMS05801

(Morón-Oset et al., 2023)

NOT suppressed by

Statement

Reference

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has short lived | RU486 conditional phenotype, non-suppressible by Scer\GAL4^{elav.Switch.PO}/ref(2)P^UAS.Tag:HA

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has visible | adult stage phenotype, non-suppressible by Atg5^RNAi.UAS.cUa, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has decreased size | adult stage phenotype, non-suppressible by Atg5^RNAi.UAS.cUa, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has short lived | RU486 conditional phenotype, non-suppressible by Atg5^RNAi.UAS.cUa, Scer\GAL4^{elav.Switch.PO}

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has visible | adult stage phenotype, non-suppressible by Rpn11^UAS.cMa, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has decreased size | adult stage phenotype, non-suppressible by Rpn11^UAS.cMa, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has short lived | RU486 conditional phenotype, non-suppressible by Rpn11^UAS.cMa/Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has visible | adult stage phenotype, non-suppressible by Rpn6^UAS.cTa, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has decreased size | adult stage phenotype, non-suppressible by Rpn6^UAS.cTa, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has short lived | RU486 conditional phenotype, non-suppressible by Rpn6^UAS.cTa, Scer\GAL4^{elav.Switch.PO}

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has visible | adult stage phenotype, non-suppressible by unc-119^GD9180/Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has decreased size | adult stage phenotype, non-suppressible by unc-119^GD9180/Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has decreased rate of adult locomotory behavior | RU486 conditional phenotype, non-suppressible by Sec22^HMS01238/Scer\GAL4^{elav.Switch.PO}

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has short lived | RU486 conditional phenotype, non-suppressible by Sec22^HMS01238/Scer\GAL4^{elav.Switch.PO}

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has visible | adult stage phenotype, non-suppressible by PEK^e01744

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has decreased size | adult stage phenotype, non-suppressible by PEK^e01744

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has short lived | RU486 conditional phenotype, non-suppressible by PEK^e01744

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has short lived | RU486 conditional phenotype, non-suppressible by Hsp22^GLC01686, Scer\GAL4^{elav.Switch.PO}

(Morón-Oset et al., 2023)

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GA^400.UAS has short lived | RU486 conditional phenotype, non-suppressible by Hsp83^HMS00899, Scer\GAL4^{elav.Switch.PO}

(Morón-Oset et al., 2023)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has eye phenotype, enhanceable by ref(2)P^HMS00938/Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has eye phenotype, enhanceable by Hsap\UNC119^UAS.Tag:HA, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has eye phenotype, enhanceable by Hsp22^GLC01686, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has eye phenotype, enhanceable by Hsp83^HMS00899, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has eye phenotype, enhanceable by ref(2)P^HMS00551/Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has eye phenotype, non-enhanceable by PEK^e01744

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has eye phenotype, non-enhanceable by Atg5^RNAi.UAS.cUa, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has eye phenotype, non-enhanceable by Rpn11^UAS.cMa, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has eye phenotype, non-enhanceable by Rpn6^UAS.cTa, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has eye phenotype, non-enhanceable by unc-119^GD9180/Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has eye phenotype, suppressible | partially by ref(2)P^UAS.Tag:HA/Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has eye phenotype, suppressible | partially by Atg1^GS10797, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has eye phenotype, suppressible | partially by InR^A1325D.UAS, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has eye phenotype, non-suppressible by PEK^e01744

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has eye phenotype, non-suppressible by Atg5^RNAi.UAS.cUa, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has eye phenotype, non-suppressible by Rpn11^UAS.cMa, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has eye phenotype, non-suppressible by Rpn6^UAS.cTa, Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS has eye phenotype, non-suppressible by unc-119^GD9180/Scer\GAL4^GMR.PFa

(Morón-Oset et al., 2023)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Zzzz\poly-GA^400.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

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