FlyBase Allele Report: Dmel\Atg5[RNAi.UAS.cUa]

General Information

Symbol

Dmel\Atg5^RNAi.UAS.cUa

Species

D. melanogaster

Name

FlyBase ID

FBal0407424

Feature type

allele

Associated gene

Dmel\Atg5

Associated Insertion(s)

Carried in Construct

P{UAS-Atg5.RNAi.U}

Also Known As

Atg5 RNAi, UAS-Atg5^RNAi, UAS-Atg5 RNAi

Key Links

Transgenic product class

RNAi_reagent

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

(FlyBase Curators, 2025-)

Progenitor genotype

Carried in construct

P{UAS-Atg5.RNAi.U}

Cytology

Description

UAS regulatory sequences drive expression of an RNAi line that targets Atg5.

(FlyBase Curators, 2025-)

FlyBase curator comment: this entry represents a transgenic construct where the particular construct used cannot be determined.

(FlyBase Curators, 2025-)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Atg5

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Maurya and Tapadia, 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^ple.PU

(Babcock et al., 2015)

abnormal oxidative stress response | adult stage, with Scer\GAL4^Tub.PU

(Wang et al., 2026)

increased mortality, with Scer\GAL4^Tub.PU

(Wang et al., 2026)

Phenotype Manifest In

dopaminergic PPL1 neuron, with Scer\GAL4^ple.PU

(Babcock et al., 2015)

lipid droplet | adult stage, with Scer\GAL4^Mhc.PK

(Yan et al., 2017)

mesothoracic tergotrochanter muscle cell, with Scer\GAL4^Mhc.PK

(Yan et al., 2017)

Detailed Description

Statement

Reference

Expression of Atg5^TRiP.cUa under the control of Scer\GAL4^ple.PU (in combination with a Dicer-2 transgene to enhance RNAi efficiency) causes significantly decreased numbers of PPL1 dopaminergic neurons after 48h at 29[o]C, as compared with controls.

(Babcock et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Atg5^RNAi.UAS.cUa, Scer\GAL4^GMR.PFa is a non-enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS

(Morón-Oset et al., 2023)

Atg5^RNAi.UAS.cUa, Scer\GAL4^GMR.PFa is a non-enhancer of decreased size | adult stage phenotype of Scer\GAL4^GMR.PFa, Zzzz\poly-GA^400.UAS