FlyBase Allele Report: Scer\GAL4[Mhc.PK]

General Information

Symbol

Scer\GAL4^Mhc.PK

Species

S. cerevisiae

Name

FlyBase ID

FBal0294941

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Mhc-GAL4.K}

Also Known As

Mhc-GAL4, MHCGal4, Mhc>Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Klein et al., 2014)

Progenitor genotype

Carried in construct

P{Mhc-GAL4.K}

Cytology

Description

A 2.5kb Mhc enhancer drives expression of GAL4.

(Klein et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

Mhc

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

larval muscle system

(Lin et al., 2015)

Additional Information

Statement

Reference

Drives expression in larval muscle.

(FlyBase Curators, 2017)

Marker for

Reflects expression of

Mhc

(Lin et al., 2015)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight | progressive, with myd^KK100114

(Sidisky et al., 2021)

abnormal locomotor behavior | third instar larval stage, with Rh50^GD3847

(Lecompte et al., 2020)

abnormal mating behavior, with Pdfr^HMJ02073

(Miao et al., 2025)

abnormal neuroanatomy | adult stage, with myd^3PM71, myd^UAS.Venus

(Sidisky et al., 2021)

abnormal neuroanatomy | adult stage, with Scer\GAL4^VGlut1-OK371, myd^3PM71, myd^UAS.Venus

(Sidisky et al., 2021)

abnormal neuroanatomy | adult stage | progressive, with myd^KK100114

(Sidisky et al., 2021)

abnormal neuroanatomy | larval stage, with mnb^{D401ter.UAS.Tag:FLAG}

(Lee et al., 2020)

abnormal neuroanatomy | larval stage, with mnb^GD13197

(Lee et al., 2020)

abnormal neuroanatomy | larval stage, with mnb^UAS.Tag:FLAG

(Lee et al., 2020)

abnormal neuroanatomy | third instar larval stage, with Vps35^GD11710

(Inoshita et al., 2017)

abnormal neurophysiology | third instar larval stage, with Vps35^GD11710

(Inoshita et al., 2017)

decreased rate of adult locomotory behavior, with Ance-3^KK106156

(Herrera and Cauchi, 2023)

decreased rate of flight behavior, with Ance-3^KK106156

(Herrera and Cauchi, 2023)

lethal, with Ret^MEN2B.UAS

(Klein et al., 2014)

lethal - all die during embryonic stage, with sls^JF01099

(Michael et al., 2024)

partially lethal, with Ance^NIG.8827R

(Herrera and Cauchi, 2023)

short lived, with ND-18^KK108847

(Foriel et al., 2018)

viable, with Ance-3^KK106156

(Herrera and Cauchi, 2023)

Phenotype Manifest In

adult fat body | adult stage | conditional, with ND-18^KK108847

(Foriel et al., 2018)

axon terminus | adult stage, with myd^KK100114

(Sidisky et al., 2021)

DLM motor neuron, with myd^KK100114

(Sidisky et al., 2021)

embryonic/larval neuromuscular junction | third instar larval stage, with Vps35^GD11710

(Inoshita et al., 2017)

indirect flight muscle cell, with ND-18^KK108847

(Foriel et al., 2018)

lipid droplet | adult stage, with Atg1^RNAi.UAS.cUa

(Yan et al., 2017)

lipid droplet | adult stage, with Atg5^RNAi.UAS.cUa

(Yan et al., 2017)

lipid droplet | adult stage, with Lsd-2^UAS.cUa

(Yan et al., 2017)

lipid droplet | adult stage, with Seipin^RNAi.UAS

(Yan et al., 2017)

lipid droplet | adult stage | progressive, with Hsc70-4^RNAi.UAS.cUa

(Yan et al., 2017)

lipid droplet | adult stage | progressive, with Lsd-2^HMS00629

(Yan et al., 2017)

mesothoracic tergotrochanter muscle cell, with Atg1^RNAi.UAS.cUa

(Yan et al., 2017)

mesothoracic tergotrochanter muscle cell, with Atg5^RNAi.UAS.cUa

(Yan et al., 2017)

mesothoracic tergotrochanter muscle cell, with Lsd-2^UAS.cUa

(Yan et al., 2017)

mesothoracic tergotrochanter muscle cell, with Seipin^RNAi.UAS

(Yan et al., 2017)

mesothoracic tergotrochanter muscle cell | progressive, with Hsc70-4^RNAi.UAS.cUa

(Yan et al., 2017)

mesothoracic tergotrochanter muscle cell | progressive, with Lsd-2^HMS00629

(Yan et al., 2017)

mitochondrial crista | adult stage, with ND-18^KK108847

(Foriel et al., 2018)

mitochondrial matrix | adult stage, with ND-18^KK108847

(Foriel et al., 2018)

mitochondrial membrane | adult stage, with ND-18^KK108847

(Foriel et al., 2018)

mitochondrion | adult stage, with ND-18^KK108847

(Foriel et al., 2018)

NMJ bouton | larval stage, with mnb^{D401ter.UAS.Tag:FLAG}

(Lee et al., 2020)

NMJ bouton | larval stage, with mnb^GD13197

(Lee et al., 2020)

NMJ bouton | larval stage, with mnb^UAS.Tag:FLAG

(Lee et al., 2020)

type I bouton | third instar larval stage, with Vps35^GD11710

(Inoshita et al., 2017)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^Mhc.PK/Akt^UAS.Exel is a suppressor | partially of atrophy | larval stage phenotype of Ncra\QF^QF2.Act5C.PL, Ras85D^V12.QUAS.cLa, scrib^{QUAS.HMS01490}

(Bakopoulos et al., 2023)

Scer\GAL4^Mhc.PK, Scer\GAL4^VGlut1-OK371, Hsap\SDF4^UAS.cSa is a suppressor | partially of abnormal flight | dominant | progressive phenotype of myd^3PM71

(Sidisky et al., 2021)

Scer\GAL4^Mhc.PK, Scer\GAL4^VGlut1-OK371, Hsap\SDF4^UAS.cSa is a suppressor | partially of abnormal neuroanatomy | adult stage | progressive phenotype of myd^3PM71

(Sidisky et al., 2021)

Scer\GAL4^Mhc.PK/Hsap\SDF4^UAS.cSa is a suppressor | partially of abnormal neuroanatomy | adult stage | progressive phenotype of myd^3PM71

(Sidisky et al., 2021)

Scer\GAL4^Mhc.PK/myo^{RNAi.2259-3503.UAS} is a suppressor of increased size | larval stage phenotype of Smox^F4

(Upadhyay et al., 2020)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Mhc.PK/Mad^JF01264 is a non-suppressor of atrophy | larval stage phenotype of Ncra\QF^QF2.Act5C.PL, Ras85D^V12.QUAS.cLa, scrib^{QUAS.HMS01490}

(Bakopoulos et al., 2023)

Scer\GAL4^Mhc.PK/Hsap\SDF4^UAS.cSa is a non-suppressor of abnormal flight | dominant | progressive phenotype of myd^3PM71

(Sidisky et al., 2021)

Phenotype Manifest In

Suppressed by

Statement

Reference

Lsd-2^UAS.cUa, Scer\GAL4^Mhc.PK has mesothoracic tergotrochanter muscle cell phenotype, suppressible | partially by HDAC6^UAS.cDa, Scer\GAL4^Mhc.PK

(Yan et al., 2017)

Lsd-2^UAS.cUa, Scer\GAL4^Mhc.PK has lipid droplet | adult stage phenotype, suppressible | partially by HDAC6^UAS.cDa, Scer\GAL4^Mhc.PK

(Yan et al., 2017)

Lsd-2^UAS.cUa, Scer\GAL4^Mhc.PK has mesothoracic tergotrochanter muscle cell phenotype, suppressible | partially by Hsc70-4^UAS.cEa

(Yan et al., 2017)

Lsd-2^UAS.cUa, Scer\GAL4^Mhc.PK has lipid droplet | adult stage phenotype, suppressible | partially by Hsc70-4^UAS.cEa

(Yan et al., 2017)

NOT suppressed by

Statement

Reference

Lsd-2^UAS.cUa, Scer\GAL4^Mhc.PK has mesothoracic tergotrochanter muscle cell phenotype, non-suppressible by HDAC6^N.UAS, Scer\GAL4^Mhc.PK

(Yan et al., 2017)

Lsd-2^UAS.cUa, Scer\GAL4^Mhc.PK has lipid droplet | adult stage phenotype, non-suppressible by HDAC6^N.UAS, Scer\GAL4^Mhc.PK

(Yan et al., 2017)

Enhancer of

Statement

Reference

Scer\GAL4^Mhc.PK/Akt^UAS.Exel is an enhancer of extracellular matrix | larval stage phenotype of Ncra\QF^QF2.Act5C.PL, Ras85D^V12.QUAS.cLa, scrib^{QUAS.HMS01490}

(Bakopoulos et al., 2023)

Suppressor of

Statement

Reference

Scer\GAL4^Mhc.PK/Akt^UAS.Exel is a suppressor | partially of A1-7 ventral longitudinal muscle 3 | larval stage phenotype of Ncra\QF^QF2.Act5C.PL, Ras85D^V12.QUAS.cLa, scrib^{QUAS.HMS01490}

(Bakopoulos et al., 2023)

Scer\GAL4^Mhc.PK/Akt^UAS.Exel is a suppressor | partially of muscle attachment site | larval stage phenotype of Ncra\QF^QF2.Act5C.PL, Ras85D^V12.QUAS.cLa, scrib^{QUAS.HMS01490}

(Bakopoulos et al., 2023)

Scer\GAL4^Mhc.PK, Scer\GAL4^VGlut1-OK371, Hsap\SDF4^UAS.cSa is a suppressor | partially of adult neuromuscular junction phenotype of myd^3PM71

(Sidisky et al., 2021)

Scer\GAL4^Mhc.PK, Scer\GAL4^VGlut1-OK371, Hsap\SDF4^UAS.cSa is a suppressor | partially of NMJ bouton | adult stage | decreased number phenotype of myd^3PM71

(Sidisky et al., 2021)

Scer\GAL4^Mhc.PK/Hsap\SDF4^UAS.cSa is a suppressor | partially of adult neuromuscular junction phenotype of myd^3PM71

(Sidisky et al., 2021)

Scer\GAL4^Mhc.PK/Hsap\SDF4^UAS.cSa is a suppressor | partially of NMJ bouton | adult stage | decreased number phenotype of myd^3PM71

(Sidisky et al., 2021)

Scer\GAL4^Mhc.PK/myo^{RNAi.2259-3503.UAS} is a suppressor of wing disc | larval stage phenotype of Smox^F4

(Upadhyay et al., 2020)

Scer\GAL4^Mhc.PK/Hsc70-4^UAS.cEa is a suppressor of mesothoracic tergotrochanter muscle cell | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

Scer\GAL4^Mhc.PK/Hsc70-4^UAS.cEa is a suppressor of lipid droplet | adult stage | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

Scer\GAL4^Mhc.PK/Lsd-2^HMS00629 is a suppressor of mesothoracic tergotrochanter muscle cell | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

Scer\GAL4^Mhc.PK/Lsd-2^HMS00629 is a suppressor of lipid droplet | adult stage | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

HDAC6^UAS.cDa, Scer\GAL4^Mhc.PK is a suppressor | partially of mesothoracic tergotrochanter muscle cell phenotype of Lsd-2^UAS.cUa, Scer\GAL4^Mhc.PK

(Yan et al., 2017)

HDAC6^UAS.cDa, Scer\GAL4^Mhc.PK is a suppressor | partially of lipid droplet | adult stage phenotype of Lsd-2^UAS.cUa, Scer\GAL4^Mhc.PK

(Yan et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Mhc.PK/Mad^JF01264 is a non-suppressor of A1-7 ventral longitudinal muscle 3 | larval stage phenotype of Ncra\QF^QF2.Act5C.PL, Ras85D^V12.QUAS.cLa, scrib^{QUAS.HMS01490}

(Bakopoulos et al., 2023)

Scer\GAL4^Mhc.PK/Mad^JF01264 is a non-suppressor of muscle attachment site | larval stage phenotype of Ncra\QF^QF2.Act5C.PL, Ras85D^V12.QUAS.cLa, scrib^{QUAS.HMS01490}

(Bakopoulos et al., 2023)

Scer\GAL4^Mhc.PK/Atg8a^{UASp.EGFP,mCherry} is a non-suppressor of mesothoracic tergotrochanter muscle cell | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

Scer\GAL4^Mhc.PK/Atg8a^{UASp.EGFP,mCherry} is a non-suppressor of lipid droplet | adult stage | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

Scer\GAL4^Mhc.PK/Hsl^UAS.EGFP is a non-suppressor of mesothoracic tergotrochanter muscle cell | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

Scer\GAL4^Mhc.PK/Hsl^UAS.EGFP is a non-suppressor of lipid droplet | adult stage | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

Scer\GAL4^Mhc.PK/bmm^UAS.cUa is a non-suppressor of mesothoracic tergotrochanter muscle cell | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

Scer\GAL4^Mhc.PK/bmm^UAS.cUa is a non-suppressor of lipid droplet | adult stage | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

Scer\GAL4^Mhc.PK/Lpin^HMJ30181 is a non-suppressor of mesothoracic tergotrochanter muscle cell | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

Scer\GAL4^Mhc.PK/Lpin^HMJ30181 is a non-suppressor of lipid droplet | adult stage | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

HDAC6^N.UAS, Scer\GAL4^Mhc.PK is a non-suppressor of mesothoracic tergotrochanter muscle cell phenotype of Lsd-2^UAS.cUa, Scer\GAL4^Mhc.PK

(Yan et al., 2017)

Hsc70-4^K71S.UAS/Scer\GAL4^Mhc.PK is a non-suppressor of mesothoracic tergotrochanter muscle cell | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

HDAC6^N.UAS, Scer\GAL4^Mhc.PK is a non-suppressor of lipid droplet | adult stage phenotype of Lsd-2^UAS.cUa, Scer\GAL4^Mhc.PK

(Yan et al., 2017)

Hsc70-4^K71S.UAS/Scer\GAL4^Mhc.PK is a non-suppressor of lipid droplet | adult stage | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

Scer\GAL4^Mhc.PK/Hsc70-4^D206S.UAS is a non-suppressor of mesothoracic tergotrochanter muscle cell | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

Scer\GAL4^Mhc.PK/Hsc70-4^D206S.UAS is a non-suppressor of lipid droplet | adult stage | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

Scer\GAL4^Mhc.PK/DnaJ-1^UAS.cKa is a non-suppressor of mesothoracic tergotrochanter muscle cell | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

Scer\GAL4^Mhc.PK/DnaJ-1^UAS.cKa is a non-suppressor of lipid droplet | adult stage | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

mTor^GL00156/Scer\GAL4^Mhc.PK is a non-suppressor of mesothoracic tergotrochanter muscle cell | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

mTor^GL00156/Scer\GAL4^Mhc.PK is a non-suppressor of lipid droplet | adult stage | progressive phenotype of HDAC6^KO

(Yan et al., 2017)

Scer\GAL4^Mhc.PK, Ret^MEN2B.UAS, Scer\GAL80^ts.αTub84B is a non-suppressor of indirect flight muscle cell phenotype of park¹/park²⁵

(Klein et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

myd^UAS.Venus, Scer\GAL4^Mhc.PK, Scer\GAL4^VGlut1-OK371 rescues myd^3PM71

(Sidisky et al., 2021)

myd^UAS.Venus/Scer\GAL4^Mhc.PK rescues myd^KK100114

(Sidisky et al., 2021)

Scer\GAL4^Mhc.PK/HDAC6^UAS.cDa rescues HDAC6^KO

(Yan et al., 2017)

Partially rescues

myd^UAS.Venus/Scer\GAL4^Mhc.PK partially rescues myd^3PM71

(Sidisky et al., 2021)

Scer\GAL4^Mhc.PK/HDAC6^N.UAS partially rescues HDAC6^KO

(Yan et al., 2017)

Fails to rescue

Scer\GAL4^Mhc.PK/sxc^UAS.cMa fails to rescue sxc^K872M

(Czajewski et al., 2024)

TBC1d7^UAS.cRa/Scer\GAL4^Mhc.PK fails to rescue TBC1d7^KO

(Ren et al., 2018)

Scer\GAL4^Mhc.PK/HDAC6^{H237A.H664A.UAS} fails to rescue HDAC6^KO

(Yan et al., 2017)

Scer\GAL4^Mhc.PK/path^A.UAS.GFP fails to rescue path^dg50/Df(3L)BSC773

(Lin et al., 2015)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

55132

P{Mhc-GAL4.K}1, w^*/FM7c

55133

w^*; P{Mhc-GAL4.K}2/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^Mhc.PK

Name Synonyms

Secondary FlyBase IDs

References (49)

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