FlyBase Allele Report: Scer\GAL4[GMR54H02]

General Information

Symbol

Scer\GAL4^GMR54H02

Species

S. cerevisiae

Name

FlyBase ID

FBal0253514

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{GMR54H02-GAL4}

Also Known As

R54H02-Gal4, GMR54H02-Gal4, 54H02-GAL4, Wrapper-Gal4, GMR54H02

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Pfeiffer et al., 2011.2.9)

Progenitor genotype

Carried in construct

P{GMR54H02-GAL4}

Cytology

Description

A 932 base pair fragment from the flanking non-coding or intronic region of wrapper is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.

(Pfeiffer et al., 2011.2.9)

Allele components

Component

Use(s)

Regulatory region(s)

GMR54H02

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- larval stage

embryonic/larval cell body glial cell

(Kremer et al., 2017)

embryonic stage 16

midline glial cell

(Banerjee et al., 2017)

larval neuron | subset

(Banerjee et al., 2017)

third instar larval stage

embryonic/larval CNS subperineurial glial cell

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell

(Coutinho-Budd et al., 2017)

larval ventral nerve cord | restricted

(Coutinho-Budd et al., 2017)

embryonic/larval Malpighian tubule

(Coutinho-Budd et al., 2017)

optic-lobe-associated cortex glial cell

(Coutinho-Budd et al., 2017)

pupal stage -- adult stage

adult brain cell body glial cell

(Bajar et al., 2022)

adult stage

adult brain cell body glial cell

(Hill et al., 2019, Konstantinides et al., 2018, Coutinho-Budd et al., 2017, Kremer et al., 2017)

Additional Information

Statement

Reference

ScerGAL4^GMR54H02 drives expression in cortex glia from mid-pupal through adult stages.

(Bajar et al., 2022)

ScerGAL4^GMR54H02 drives expression in midline glial cells as well as unidentified neurons in the embryo.

(Banerjee et al., 2017)

ScerGAL4^GMR54H02 drives expression in adult cortex glial cells; expression is not observed in the lamina. ScerGAL4^GMR54H02 also drives expression in cortex glia in the embryonic and larval CNS.

(Kremer et al., 2017)

Drives expression in cell body glial cells (cortex glia) at larval through adult stages, and in midline glial cells in late embryos.

(FlyBase Curators, 2017)

Marker for

adult brain cell body glial cell

(Bajar et al., 2022)

Reflects expression of

wrapper

(Konstantinides et al., 2018, Banerjee et al., 2017)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal smell perception, with Hmgs^KK107372

(Silva et al., 2022)

decreased cell number | adult stage, with vex^HMC06376

(Davis et al., 2022)

decreased cell number | larval stage, with ct^HMS00924

(Yadav et al., 2024)

decreased efficacy of long-term memory | adult stage, with Akt^HMS00007

(de Tredern et al., 2021)

decreased efficacy of long-term memory | adult stage, with Alat^GD9174

(Rabah et al., 2023)

decreased efficacy of long-term memory | adult stage, with Alat^HMC05124

(Rabah et al., 2023)

decreased efficacy of long-term memory | adult stage, with Ctet\tetX^TNT-LC.UAS

(de Tredern et al., 2021)

decreased efficacy of long-term memory | adult stage, with Glug^GD2869

(Rabah et al., 2023)

decreased efficacy of long-term memory | adult stage, with Glug^NIG.31100R

(Rabah et al., 2023)

decreased efficacy of long-term memory | adult stage, with Glut1^GD4552

(de Tredern et al., 2021)

decreased efficacy of long-term memory | adult stage, with Glut1^KK108683

(de Tredern et al., 2021)

decreased efficacy of long-term memory | adult stage, with Ilp4^HMS00547

(de Tredern et al., 2021)

decreased efficacy of long-term memory | adult stage, with Ilp4^HMS02660

(de Tredern et al., 2021)

decreased efficacy of long-term memory | adult stage, with InR^GD104

(de Tredern et al., 2021)

decreased efficacy of long-term memory | adult stage, with InR^HMS03166

(de Tredern et al., 2021)

decreased efficacy of long-term memory | adult stage, with nAChRα7^JF02570

(de Tredern et al., 2021)

decreased efficacy of long-term memory | adult stage, with nAChRα7^KK108471

(de Tredern et al., 2021)

decreased efficacy of long-term memory | adult stage, with nebu^GD2444

(Rabah et al., 2023)

decreased efficacy of long-term memory | adult stage, with nebu^HMS01072

(Rabah et al., 2023)

decreased efficacy of long-term memory | adult stage, with Pfk^HMS01324

(Rabah et al., 2023)

decreased efficacy of long-term memory | adult stage, with Pfk^KK101887

(Rabah et al., 2023)

decreased efficacy of long-term memory | adult stage, with Pi3K92E^JF02770

(de Tredern et al., 2021)

decreased efficacy of long-term memory | adult stage | heat sensitive, with shi^1.UAS

(de Tredern et al., 2021)

decreased efficacy of medium-term memory | adult stage, with Alat^GD9174

(Rabah et al., 2023)

decreased efficacy of medium-term memory | adult stage, with Alat^HMC05124

(Rabah et al., 2023)

decreased efficacy of medium-term memory | adult stage, with Glug^GD2869

(Rabah et al., 2023)

decreased efficacy of medium-term memory | adult stage, with Glug^NIG.31100R

(Rabah et al., 2023)

decreased efficacy of medium-term memory | adult stage, with Pfk^KK101887

(Rabah et al., 2023)

decreased efficacy of memory | adult stage | calorie restriction conditional, with AMPKα^HMC04979

(Silva et al., 2022)

decreased efficacy of memory | adult stage | calorie restriction conditional, with AMPKα^JF01951

(Silva et al., 2022)

decreased efficacy of memory | adult stage | calorie restriction conditional, with bmm^GD5139

(Silva et al., 2022)

decreased efficacy of memory | adult stage | calorie restriction conditional, with bmm^JF01946

(Silva et al., 2022)

decreased efficacy of memory | adult stage | calorie restriction conditional, with chk^GD1829

(Silva et al., 2022)

decreased efficacy of memory | adult stage | calorie restriction conditional, with Hmgs^HMC04928

(Silva et al., 2022)

decreased efficacy of memory | adult stage | calorie restriction conditional, with Hmgs^KK107372

(Silva et al., 2022)

decreased efficacy of memory | adult stage | calorie restriction conditional, with whd^HMS00040

(Silva et al., 2022)

decreased efficacy of memory | adult stage | calorie restriction conditional, with whd^KK100935

(Silva et al., 2022)

decreased rate of adult locomotory behavior | recessive, with vex^HMC06376

(Davis et al., 2022)

partially lethal - majority die | male, with dths^HMS03377

(Zhang et al., 2024)

viable | female, with dths^HMS03377

(Zhang et al., 2024)

Phenotype Manifest In

adult brain | calorie restriction conditional, with AMPKα^JF01951

(Silva et al., 2022)

adult brain | calorie restriction conditional, with bmm^JF01946

(Silva et al., 2022)

adult brain | calorie restriction conditional, with chk^GD1829

(Silva et al., 2022)

adult brain | calorie restriction conditional, with Hmgs^KK107372

(Silva et al., 2022)

adult brain | calorie restriction conditional, with whd^HMS00040

(Silva et al., 2022)

adult dopaminergic neuron | decreased number, with vex^HMC06376

(Davis et al., 2022)

dopaminergic PPL1 neuron | adult stage | decreased number, with vex^HMC06376

(Davis et al., 2022)

embryonic/larval cell body glial cell | second instar larval stage, with αSnap^KK107910

(Coutinho-Budd et al., 2017)

lipid droplet | adult stage | calorie restriction conditional, with AMPKα^JF01951

(Silva et al., 2022)

lipid droplet | adult stage | calorie restriction conditional, with bmm^JF01946

(Silva et al., 2022)

lipid droplet | adult stage | calorie restriction conditional, with chk^GD1829

(Silva et al., 2022)

lipid droplet | adult stage | calorie restriction conditional, with Hmgs^KK107372

(Silva et al., 2022)

lipid droplet | adult stage | calorie restriction conditional, with whd^HMS00040

(Silva et al., 2022)

neuroblast | decreased number | larval stage, with ct^HMS00924

(Yadav et al., 2024)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ilp4^HMS02660, Scer\GAL4^GMR54H02 has decreased efficacy of long-term memory | adult stage phenotype, suppressible by Akt^{ΔPH.UAS.Tag:Myr(Unk)}, Scer\GAL4^GMR54H02

(de Tredern et al., 2021)

Suppressor of

Statement

Reference

Akt^{ΔPH.UAS.Tag:Myr(Unk)}, Scer\GAL4^GMR54H02 is a suppressor of decreased efficacy of long-term memory | adult stage phenotype of Ilp4^HMS02660, Scer\GAL4^GMR54H02

(de Tredern et al., 2021)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Scer\GAL4^GMR54H02/Cpes^UAS.Tag:V5 fails to rescue Cpes¹

(Chen et al., 2022)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

45784

w¹¹¹⁸; P{GMR54H02-GAL4}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^GMR54H02

Name Synonyms

Secondary FlyBase IDs

References (44)

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